Extract from the Register of European Patents

About this file: EP2404929

EP2404929 - Glucanases, nucleic acids encoding them and methods for making and using them [Right-click to bookmark this link]
StatusNo opposition filed within time limit
Status updated on  24.04.2015
Database last updated on 13.07.2017
Most recent event   Tooltip15.07.2016Lapse of the patent in a contracting state
New state(s): LU, TR		published on 17.08.2016  [2016/33]
Applicant(s)For all designated states
BP Corporation North America Inc.
501 Westlake Park Boulevard
Houston, TX 77079 / US
For all designated states
Syngenta Participations AG
Schwarzwaldallee 215
4058 Basel / CH
[N/P]
Former [2014/24]For all designated states
BP Corporation North America Inc.
501 Westlake Park Boulevard
Houston, TX 77079 / US
For all designated states
Syngenta Participations AG
Schwarzwaldallee 215
4058 Basel / CH
Former [2013/37]For all designated states
Verenium Corporation
3550 John Hopkins Ct.
San Diego, CA 92121 / US
For all designated states
Syngenta Participations AG
Schwarzwaldallee 215
4058 Basel / CH
For all designated states
BP Corporation North America Inc.
501 Westlake Park Boulevard
Houston, TX 77079 / US
Former [2013/12]For all designated states
Verenium Corporation
3550 John Hopkins Ct.
San Diego, CA 92121 / US
For all designated states
Syngenta Participations AG
Schwarzwaldallee 215
4058 Basel / CH
Former [2012/02]For all designated states
Verenium Corporation
4955 Directors Place
San Diego, CA 92121 / US
For all designated states
Syngenta Participations AG
Schwarzwaldallee 215
4058 Basel / CH
Inventor(s)01 / Steer, Brian
1136 Missouri Street
San Diego, CA California CA 92109 / US
02 / Callen, Walter
3469 Stetson Avenue
San Diego, CA California CA 92122 / US
03 / Healey, Shaun
3380 Harding Street, 7A
Carlsbad, CA California CA 92008 / US
04 / Pulliam, Derrick
Syngenta Biotechnology, Inc.
3054 Cornwallis Road
North Carolina, NC North Carolina NC 27709 / US
 [2012/02]
Representative(s)Wakerley, Helen Rachael
Reddie & Grose LLP The White Chapel Building
10 Whitechapel High Street
London E1 8QS / GB
[N/P]
Former [2014/25]Wakerley, Helen Rachael
Reddie & Grose LLP
16 Theobalds Road
London WC1X 8PL / GB
Former [2012/02]Wakerley, Helen Rachael
Reddie & Grose 16 Theobalds Road London
Greater London WC1X 8PL / GB
Application number, filing date11176104.502.07.2004
[2012/02]
Priority number, dateUS2003048472502.07.2003         Original published format: US 484725
[2012/02]
Filing languageEN
Procedural languageEN
PublicationType: A1 Application with search report 
No.:EP2404929
Date:11.01.2012
Language:EN
[2012/02]
Type: B1 Patent specification 
No.:EP2404929
Date:18.06.2014
Language:EN
[2014/25]
International and Supplementary
search report(s)		Supplementary European search report -
dispatched on:		EP14.12.2011
ClassificationInternational:C07H21/04, C12P21/06, C12N9/42
[2012/02]
Designated contracting statesAT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HU,   IE,   IT,   LI,   LU,   MC,   NL,   PL,   PT,   RO,   SE,   SI,   SK,   TR [2012/02]
TitleGerman:Glucanasen, Nukleinsäuren, die diese codieren, und Verfahren zu deren Herstellung und Verwendung[2012/02]
English:Glucanases, nucleic acids encoding them and methods for making and using them[2012/02]
French:Glucanases, acides nucléiques les codant et leurs procédés de fabrication et d'utilisation[2012/02]
Examination procedure25.08.2011Examination requested  [2012/02]
11.07.2012Amendment by applicant (claims and/or description)
15.10.2012Despatch of a communication from the examining division (Time limit: M04)
12.02.2013Reply to a communication from the examining division
25.07.2013Communication of intention to grant the patent
03.12.2013Disapproval of the communication of intention to grant the patent by the applicant or resumption of examination proceedings by the EPO
02.01.2014Communication of intention to grant the patent
06.05.2014Fee for grant paid
06.05.2014Fee for publishing/printing paid
06.05.2014Receipt of the translation of the claim(s)
Parent application(s)   TooltipEP04777548.1  / EP1641910
Divisional application(s)The date of the Examining Division's first communication in respect of the earliest application for which a communication has been issued (EP20040777548) is  30.07.2009
Opposition(s)19.03.2015No opposition filed within time limit [2015/22]
Fees paidRenewal fee
25.08.2011Renewal fee patent year 03
25.08.2011Renewal fee patent year 04
25.08.2011Renewal fee patent year 05
25.08.2011Renewal fee patent year 06
25.08.2011Renewal fee patent year 07
25.08.2011Renewal fee patent year 08
25.07.2012Renewal fee patent year 09
29.07.2013Renewal fee patent year 10
Lapses during opposition  TooltipHU02.07.2004
AT18.06.2014
BE18.06.2014
BG18.06.2014
CY18.06.2014
CZ18.06.2014
DK18.06.2014
EE18.06.2014
ES18.06.2014
FI18.06.2014
IT18.06.2014
MC18.06.2014
NL18.06.2014
PL18.06.2014
RO18.06.2014
SE18.06.2014
SI18.06.2014
SK18.06.2014
TR18.06.2014
IE02.07.2014
LU02.07.2014
CH31.07.2014
LI31.07.2014
GR19.09.2014
PT20.10.2014
[2016/33]
Former [2016/32]HU02.07.2004
AT18.06.2014
BE18.06.2014
BG18.06.2014
CY18.06.2014
CZ18.06.2014
DK18.06.2014
EE18.06.2014
ES18.06.2014
FI18.06.2014
IT18.06.2014
MC18.06.2014
NL18.06.2014
PL18.06.2014
RO18.06.2014
SE18.06.2014
SI18.06.2014
SK18.06.2014
IE02.07.2014
CH31.07.2014
LI31.07.2014
GR19.09.2014
PT20.10.2014
Former [2016/25]AT18.06.2014
BE18.06.2014
BG18.06.2014
CY18.06.2014
CZ18.06.2014
DK18.06.2014
EE18.06.2014
ES18.06.2014
FI18.06.2014
IT18.06.2014
MC18.06.2014
NL18.06.2014
PL18.06.2014
RO18.06.2014
SE18.06.2014
SI18.06.2014
SK18.06.2014
IE02.07.2014
CH31.07.2014
LI31.07.2014
GR19.09.2014
PT20.10.2014
Former [2015/37]AT18.06.2014
BE18.06.2014
CY18.06.2014
CZ18.06.2014
DK18.06.2014
EE18.06.2014
ES18.06.2014
FI18.06.2014
IT18.06.2014
MC18.06.2014
NL18.06.2014
PL18.06.2014
RO18.06.2014
SE18.06.2014
SI18.06.2014
SK18.06.2014
IE02.07.2014
CH31.07.2014
LI31.07.2014
GR19.09.2014
PT20.10.2014
Former [2015/36]AT18.06.2014
BE18.06.2014
CY18.06.2014
CZ18.06.2014
DK18.06.2014
EE18.06.2014
ES18.06.2014
FI18.06.2014
IT18.06.2014
MC18.06.2014
NL18.06.2014
PL18.06.2014
RO18.06.2014
SE18.06.2014
SI18.06.2014
SK18.06.2014
CH31.07.2014
LI31.07.2014
GR19.09.2014
PT20.10.2014
Former [2015/30]AT18.06.2014
BE18.06.2014
CY18.06.2014
CZ18.06.2014
DK18.06.2014
EE18.06.2014
ES18.06.2014
FI18.06.2014
IT18.06.2014
MC18.06.2014
NL18.06.2014
PL18.06.2014
RO18.06.2014
SE18.06.2014
SK18.06.2014
CH31.07.2014
LI31.07.2014
GR19.09.2014
PT20.10.2014
Former [2015/22]AT18.06.2014
CY18.06.2014
CZ18.06.2014
DK18.06.2014
EE18.06.2014
ES18.06.2014
FI18.06.2014
IT18.06.2014
MC18.06.2014
NL18.06.2014
PL18.06.2014
RO18.06.2014
SE18.06.2014
SK18.06.2014
CH31.07.2014
LI31.07.2014
GR19.09.2014
PT20.10.2014
Former [2015/20]AT18.06.2014
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CZ18.06.2014
EE18.06.2014
ES18.06.2014
FI18.06.2014
MC18.06.2014
NL18.06.2014
PL18.06.2014
RO18.06.2014
SE18.06.2014
SK18.06.2014
CH31.07.2014
LI31.07.2014
GR19.09.2014
PT20.10.2014
Former [2015/18]AT18.06.2014
CY18.06.2014
CZ18.06.2014
EE18.06.2014
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FI18.06.2014
MC18.06.2014
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PL18.06.2014
RO18.06.2014
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SK18.06.2014
GR19.09.2014
PT20.10.2014
Former [2015/12]AT18.06.2014
CY18.06.2014
CZ18.06.2014
EE18.06.2014
ES18.06.2014
FI18.06.2014
NL18.06.2014
PL18.06.2014
RO18.06.2014
SE18.06.2014
SK18.06.2014
GR19.09.2014
PT20.10.2014
Former [2015/11]CY18.06.2014
CZ18.06.2014
EE18.06.2014
ES18.06.2014
FI18.06.2014
PL18.06.2014
RO18.06.2014
SE18.06.2014
SK18.06.2014
GR19.09.2014
PT20.10.2014
Former [2015/10]CY18.06.2014
CZ18.06.2014
EE18.06.2014
ES18.06.2014
FI18.06.2014
SE18.06.2014
SK18.06.2014
GR19.09.2014
Former [2014/50]CY18.06.2014
FI18.06.2014
SE18.06.2014
GR19.09.2014
Former [2014/49]CY18.06.2014
FI18.06.2014
Former [2014/47]CY18.06.2014
Documents cited:SearchJPH07203960  [ ] (KAO CORP);
 [A]WO9824799  (DIVERSA CORP [US], et al) [A] 1-18;
 [A]WO02099091  (NOVOZYMES AS [DK], et al) [A] 1-18
 [I]  - "Full length Bacillus sp. alkaline cellulase.", DATABASE Geneseq, (19960410), Database accession no. AAR77395, URL: EBI, XP002664520 [I] 1-18 * abstract *
by applicantUS5217879
 US4458066
 US5721118
 US6025155
 US4962028
 US5633440
 US5608148
 US5602321
 US5589583
 US5750870
 EP0659215
 WO9403612
 US4683195
 US4683202
 WO9703211
 WO9639154
 US4987071
 US6506559
 US6511824
 US6515109
 US6489127
 US6361974
 US5830696
 US6291242
 US6287862
 US6287861
 US5955358
 US5830721
 US5824514
 US5811238
 US5605793
 US5834252
 US5837458
 WO9522625
 WO9633207
 WO9720078
 WO9735966
 WO9941402
 WO9941383
 WO9941369
 WO9941368
 EP0752008
 EP0932670
 WO9923107
 WO9921979
 WO9831837
 WO9827230
 WO0000632
 WO0009679
 WO9842832
 WO9929902
 WO9841653
 WO9841622
 WO9842727
 US6379964
 US6319714
 US6368861
 US6376246
 US6423542
 US6426224
 US6436675
 US6177263
 US6153410
 US6280926
 US5939250
 US5965408
 WO9116427
 US5795737
 US6211428
 US5387742
 US6187992
 US6309872
 US5015580
 US5681730
 US5712135
 US2003215812
 US2003119093
 US5661021
 US5795764
 US6376445
 US6420331
 US5693518
 US5885819
 US6200797
 US6586209
 US6682923
 US2002001809
 US2002080350
 WO0231203
 WO0244336
 US6277628
 US6277489
 US6261776
 US6258606
 US6054270
 US6048695
 US6045996
 US6022963
 US6013440
 US5965452
 US5959098
 US5856174
 US5830645
 US5770456
 US5632957
 US5556752
 US5143854
 US5807522
 US5800992
 US5744305
 US5700637
 US5434049
 WO9951773
 WO9909217
 WO9746313
 WO9617958
 US2001018642
 US2001019827
 US2001016322
 US2001014449
 US2001014448
 US2001012537
 US2001008765
 US4946778
 WO0229032
 WO0196551
 US6057103
 US6413928
 US6399561
 US6365561
 US6380147
 US6387690
 US6333301
 US6329333
 US6326341
 US6297038
 US6309871
 US6204232
 US6197070
 US5856164
 WO9701629
 US4404128
 US4261868
 US5204015
 US5786316
 US6261828
 US6077316
 US6024766
 US6021536
 US6017731
 US5980581
 US2002142438
 US6423524
 US6083733
 US6140095
 US6346407
 US5202249
 US5179021
 US5116746
 US5407827
 US5405769
 US5395765
 US5369024
 US5457045
 US5434071
 US5498534
 US5591304
 US5645686
 US5725732
 US5759840
 US5834301
 US5871730
 US6057438
 US5486468
 US5770012
 US6245546
 US6399123
 US5830732
 US5709796
 US6264925
 US5762991
 US5536650
 US5405624
 US5021246
 US4788066
 WO0049890
 WO9903497
 US5624678
 WO0043496
 WO8100857
 US5108765
 US5306633
 US5432074
 US5429828
 US5612055
 US5720971
 US5981233
 US5948667
 US6099844
 US6132727
 US6132716
 US5760211
 US5622738
 US4960705
 US5023176
 US4746517
 US4144354
 US5874274
 US6036981
 US4725544
 WO9946362
 WO0164830
 WO0130161
 WO9203541
 WO9613568
 WO9623579
 WO9732480
 WO9840721
 US6054267
 US6030779
 US6368798
 US6344328
    - KOHLMANN, ADV. SPACE RES., (1996), vol. 18, pages 251 - 265,
    - PEREZ, INT MICROBIOL., (2002), vol. 5, pages 53 - 63,
    - W.E. PAUL,, Fundamental Immunology, Third Edition,, RAVEN PRESS, (1993),
    - WILSON, J. IMMUNOL. METHODS, (1994), vol. 175, pages 267 - 273,
    - YARMUSH, J. BIOCHEM. BIOPHYS. METHODS, (1992), vol. 25, pages 85 - 97,
    - WARD ET AL., NATURE, (1989), vol. 341, pages 544 - 546,
    - MATA, TOXICOL. APPL. PHARMACOL., (1997), vol. 144, pages 189 - 197,
    - STRAUSS-SOUKUP, BIOCHEMISTRY, (1997), vol. 36, pages 8692 - 8698,
    - SAMSTAG, ANTISENSE NUCLEIC ACID DRUG DEV, (1996), vol. 6, pages 153 - 156,
    - CREIGHTON, T.E., Proteins -- Structure and Molecular Properties 2nd Ed.,, W.H. FREEMAN AND COMPANY, (1993),
    - B.C. JOHNSON,, Posttranslational Covalent Modification of Proteins, ACADEMIC PRESS, (1983), pages 1 - 12,
    - MERRIFIELD, R. B., J. AM. CHEM. SOC., (1963), vol. 85, pages 2149 - 2154,
    - STEWART, J. M., YOUNG, J. D., Solid Phase Peptide_Synthesis, 2nd Ed.,, PIERCE CHEMICAL CO., pages 11 - 12,
    - H. M. GEYSEN ET AL., PROC. NATL ACAD. SCI., (1984), vol. 81, page 3998,
    - B. LEWIN, Genes VI, OXFORD UNIVERSITY PRESS, (1997), page 214,
    - ADAMS, J. AM. CHEM. SOC., (1983), vol. 105, page 661,
    - BELOUSOV, NUCLEIC ACIDS RES., (1997), vol. 25, pages 3440 - 3444,
    - FRENKEL, FREE RADIO. BIOL. MED., (1995), vol. 19, pages 373 - 380,
    - BLOMMERS, BIOCHEMISTRY, (1994), vol. 33, pages 7886 - 7896,
    - NARANG, METH. ENZYMOL, (1979), vol. 68, page 90,
    - BROWN, METH. ENZYMCL., (1979), vol. 68, page 109,
    - BEAUCAGE, TETRA. LEIT., (1981), vol. 22, page 1859,
    - SAMBROOK,, MOLECULAR CLONING: A LABORATORY MANUAL, COLD SPRING HARBOR LABORATORY, (1989), vol. 1-3,
    - AUSUBEL,, CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, JOHN WILEY & SONS, INC., (1997),
    - "LABORATORY TECHNIQUES IN BIOCHEMISTRY AND MOLECULAR BIOLOGY: HYBRIDIZATION WITH NUCLEIC ACID PROBES", TIJSSEN,, Theory and Nucleic Acid Preparation, ELSEVIER, (1993),
    - ROSENFELD, NAT. GENET., (1997), vol. 15, pages 333 - 335,
    - WOON, GENOMICS, (1998), vol. 50, pages 306 - 316,
    - KERN, BIOTECHNIQUES, (1997), vol. 23, pages 120 - 124,
    - WILLIAMS, BIOCHEMISTRY, (1995), vol. 34, pages 1787 - 1797,
    - DOBELI, PROTEIN EXPR. PURIF., (1998), vol. 12, pages 404 - 414,
    - KROLL, DNA CELL. BIOL., (1993), vol. 12, pages 441 - 53,
    - HUANG, PLANT MOL, BIOL., (1996), vol. 33, pages 125 - 139,
    - ZHONG, MOL. GEN. GENET., (1996), vol. 251, pages 196 - 203,
    - SOLOCOMBE, PLANT PHYSIOL., (1994), vol. 104, pages 1167 - 1176,
    - MARTINEZ, J. MOL. BIOL, (1989), vol. 208, pages 551 - 561,
    - MANJUNATH, PLANT MOL. BIOL., (1997), vol. 33, pages 97 - 112,
    - KUMAGAI, PROC. NATL. ACAD. SCI. USA, (1995), vol. 92, pages 1679 - 1683,
    - VERDAGUER, PLANT MOL. BIOL., (1996), vol. 31, pages 1129 - 1139,
    - KIRCH, PLANT MOL. BIOL., (1997), vol. 33, pages 897 - 909,
    - BLAZQUEZ, PLANT CELL, (1998), vol. 10, pages 791 - 800,
    - CARDON, PLANT J, (1997), vol. 12, pages 367 - 77,
    - MANDEL, PLANT MOLECULAR BIOLOGY, (1995), vol. 29, pages 995 - 1004,
    - JOHN, PROC. NATL. ACAD. SCI. USA, (1997), vol. 89, pages 5769 - 5773,
    - DELISLE, INT. REV. CYTOL., (1990), vol. 123, pages 39 - 60,
    - BUSK, PLANT J., (1997), vol. 11, pages 1285 - 1295,
    - GUERRERO, MOL. GEN. GENET., (1990), vol. 224, pages 161 - 168,
    - BLUME, PLANT J., (1997), vol. 12, pages 731 - 746,
    - FICKER, PLANT MOL. BIOL., (1997), vol. 35, pages 425 - 431,
    - REISER, CELL, (1995), vol. 83, pages 735 - 742,
    - LIU, PLANT PHYSIOL., (1997), vol. 115, pages 397 - 407,
    - CHEN, PLANT J., (1996), vol. 10, pages 955 - 966,
    - STREIT, MOL. PLANT MICROBE INTERACT., (1997), vol. 10, pages 933 - 937,
    - SHEEN, SCIENCE, (1996), vol. 274, pages 1900 - 1902,
    - DE VEYLDER, PLANT CELL PHYSIOL., (1997), vol. 38, pages 568 - 577,
    - MASGRAU, PLANT J., (1997), vol. 11, pages 465 - 473,
    - STANGE, PLANT J., (1997), vol. 11, pages 1315 - 1324,
    - COVEY, PROC. NATL. ACAD. SCI. USA, (1990), vol. 87, pages 1633 - 1637,
    - ANGELL, EMBO J., (1997), vol. 16, pages 3675 - 3684,
    - CASPER, GENE, (1996), vol. 173, pages 69 - 73,
    - HILLMAN, VIROLOGY, (1989), vol. 169, pages 42 - 50,
    - DOLJA, VIROLOGY, (1997), vol. 234, pages 243 - 252,
    - MORINAGA, MICROBIOL IMMUNOL., (1993), vol. 37, pages 471 - 476,
    - CECCHINI, MOL. PLANT MICROBE INTERACT., (1997), vol. 10, pages 1094 - 1101,
    - RUBIN, MOL. CELL. BIOL., (1997), vol. 17, pages 6294 - 6302,
    - KUNZE, CURR. TOP. MICROBIOL. IMMUNOL., (1996), vol. 204, pages 161 - 194,
    - SCHLAPPI, PLANT MOL. BIOL., (1996), vol. 32, pages 717 - 725,
    - AUSUBEL ET AL., Current Protocols in Molecular Biology, JOHN WILEY 503 SONS, INC., (1997),
    - SAMBROOK ET AL., Molecular Cloning: A Laboratory Manual, 2nd Ed.,, COLD SPRING HARBOR LABORATORY PRESS, (1989),
    - SAMBROOK ET AL., Molecular Cloning: A Laboratory Manual 2nd Ed.., COLD SPRING HARBOR, (1989),
    - WEISING, ANN. REV. GENET., (1988), vol. 22, pages 421 - 477,
    - DAVIS, L., DIBNER, M., BATTEY, I., BASIC METHODS IN MOLECULAR BIOLOGY, (1986),
    - GURTU, BIOCHEM. BIOPHYS. RES, COMMUM., (1996), vol. 229, pages 295 - 8,
    - SANDERS, DEV. BIOL. STAND., (1987), vol. 66, pages 55 - 63,
    - MECHALY, A., SHOHAM, Y., "Overexpression and single-step purification of a thermostable glucanase from Bacillus stearothermophilus T-6", J. BIOTECHNOL., (1996), vol. 51, pages 259 - 64,
    - LÜTHI, E., JASMAT, N.B., BERGQUIST, P.L., "Endoglucanase from the extremely thermophilic bacterium Caldocellum saccharolyticum: overexpression of the gene in Escherichia coli and characterization of the gene product", APPL. ENVIRON. MICROBIOL., (1990), vol. 56, pages 2677 - 83,
    - SUNG, W.L., LUK, C.K., ZAHAB, D.M., WAKARCHUK, W., "Overexpression of the Bacillus subtilis and circulans endoglucanases in Escherichia coli", PROTEIN EXPR. PURIF., (1993), vol. 4, pages 200 - 6,
    - GLUZMAN, CELL, (1981), vol. 23, page 175,
    - INNIS,, PCR PROTOCOLS, A GUIDE TO METHODS AND APPLICATIONS, ACADEMIC PRESS, (1990),
    - INNIS., PCR STRATEGIES, ACADEMIC PRESS, INC., (1995),
    - WU, GENOMICS, (1989), vol. 4, page 560,
    - LANDEGREN, SCIENCE, (1988), vol. 241, page 1077,
    - BARRINGER, GENE, (1990), vol. 89, page 117,
    - KWOH, PROC. NATL. ACAD. SCI. USA, (1989), vol. 86, page 1173,
    - GUATELLI, PROC. NATL. ACAD. SCI. USA, (1990), vol. 87, page 1874,
    - SMITH, J. CLIN. MICROBIAL., (1997), vol. 35, pages 1477 - 1491,
    - BURG, MOL. CELL. PROBES, (1996), vol. 10, pages 257 - 271,
    - BERGER, METHODS ENZYMOL., (1987), vol. 152, pages 307 - 316,
    - SOOKNANAN, BIOTECHNOLOGY, (1995), vol. 13, pages 563 - 564,
    - PEARSON, LIPMAN, PROC. NATL. ACAD. SCI. USA, (1988), vol. 85, no. 8, pages 2444 - 2448,
    - ALTSCHUL ET AL., J. MOL. BIOL., (1990), vol. 215, no. 3, pages 403 - 410,
    - THOMPSON ET AL., NUCLEIC ACIDS RES., (1994), vol. 22, no. 2, pages 4673 - 4680,
    - HIGGINS ET AL., METHODS ENZYMOL., (1996), vol. 266, pages 383 - 402,
    - ALTSCHUL ET AL., NATURE GENETICS, (1993), vol. 3, pages 266 - 272,
    - SMITH, WATERMAN, ADV. APPL. MATH., (1981), vol. 2, page 482,
    - NEEDLEMAN, WUNSCH, J. MOL. BIOL, (1970), vol. 48, page 443,
    - LIPMAN, PROC. NAT'1. ACAD. SCI. USA, (1988), vol. 85, page 2444,
    - ALTSCHUL ET AL., NUC. ACIDS RES., (1977), vol. 25, pages 3389 - 3402,
    - ALTSCHUL ET AL., J. MOL. BIOL., (1990), vol. 215, pages 403 - 410,
    - HENIKOFF, HENIKOFF, PROC. NATL. ACAD. SCI. USA, (1989), vol. 89, page 10915,
    - KARLIN, ALTSCHUL, PROC. NATL. ACAD. SCI. USA, (1993), vol. 90, page 5873,
    - GONNET ET AL., SCIENCE, (1992), vol. 256, pages 1443 - 1445,
    - HENIKOFF, HENIKOFF, PROTEINS, (1993), vol. 17, pages 49 - 61,
    - SCHWARTZ AND DAYHOFF,, Matrices for Detecting Distance Relationships: Atlas of Protein Sequence and Structure, NATIONAL BIOMEDICAL RESEARCH FOUNDATION, (1978),
    - STRYER, LUBERT, Biochemistry. 3rd Ed., W. H FREEMAN & CO.,
    - ALTSCHUL ET AL., J. MOL. BIOL., (1990), vol. 215, page 403,
    - PEARSON, LIPMAN, PROC. NATL. ACAD. SCI. USA, (1988), vol. 85, page 2444,
    - BRUTLAG ET AL., COMP. APP. BIOSCI., (1990), vol. 6, pages 237 - 245,
    - AUSUBEL ET AL., Current Protopols in Molecular Biology, JOHN WILEY 503 SONS, INC., (1997),
    - BARANY, F., "The Ligase Chain Reaction in a PCR World", PCR METHODS AND APPLICATIONS, (1991), vol. 1, pages 5 - 16,
    - E. FAHY ET AL., "Self-sustained Sequence Replication (3SR): An Isothermal Transcription-based Amplification System Alternative to PCR", PCR METHODS AND APPLICATIONS, (1991), vol. 1, pages 25 - 33,
    - WALKER G.T. ET AL., "Strand Displacement Amplication-an Isothermal in vitro DNA Amplification Technique", NUCLEIC ACID RESEARCH, (1992), vol. 20, pages 1691 - 1696,
    - HO, METHODS ENZYMOL, (2000), vol. 314, pages 168 - 183,
    - SMITH, EUR. J. PHARM. SCI., (2000), vol. 11, pages 191 - 198,
    - MATA, TOXICOL APPL PHARMACOL, (1997), vol. 144, pages 189 - 497,
    - AGRAWAL, Antisense Therapeutics, HUMANA PRESS, (1996),
    - GOLD, J. OF BIOL. CHEM., (1995), vol. 270, pages 13581 - 13584,
    - ROSSI, AIDS RESEARCH AND HUMAN RETROVIRUSES, (1992), vol. 8, page 183,
    - HAMPEL, BIOCHEMISTRY, (1989), vol. 28, page 4929,
    - HAMPEL, NUC. ACIDS RES., (1990), vol. 18, page 299,
    - PERROTTA, BIOCHEMISTRY, (1992), vol. 31, page 16,
    - GUERRIER-TAKADA, CELL, (1983), vol. 35, page 849,
    - SHUEY, DRUG DISCOV. TODAY, (2002), vol. 7, pages 1040 - 1046,
    - RICE, PROC. NATL. ACAD. SCI. USA, (1992), vol. 89, pages 5467 - 5471,
    - CRAMERI, BIOTECHNIQUES, (1995), vol. 18, pages 194 - 196,
    - STEMMER, "Molecular breeding of viruses for targeting and other clinical properties", TUMOR TARGETING, (1999), vol. 4, pages 1 - 4,
    - NESS, NATURE BIOTECHNOLOGY, (1999), vol. 17, pages 893 - 896,
    - CHANG, "Evolution of a cytokine using DNA family shuffling", NATURE BIOTECHNOLOGY, (1999), vol. 17, pages 793 - 797,
    - MINSHULL, "Protein evolution by molecular breeding", CURRENT OPINION IN CHEMICAL BIOLOGY, (1999), vol. 3, pages 284 - 290,
    - CHRISTIANS, "Directed evolution of thymidme kinase for AZT phosphorylation using DNA family shuffling", NATURE BIOTECHNOLOGY, (1999), vol. 17, pages 259 - 264,
    - CRAMERI, "DNA shuffling of a family of genes from diverse species accelerates directed evolution", NATURE, (1998), vol. 391, pages 288 - 291,
    - CRAMERI, "Molecular evolution of an arsenate detoxification pathway by DNA shuffing", NATURE BIOTECHNOLOGY, (1997), vol. 15, pages 436 - 438,
    - ZHANG, "Directed evolution of an effective fucosidase from a galactosidase by DNA shuffling and screening", PROC. NATL. ACAD. SCI. USA, (1997), vol. 94, pages 4504 - 4509,
    - PATTEN ET AL., "Applications of DNA Shuffling to Pharmaceuticals and Vaccines", CURRENT OPINION IN BIOTECHNOLOGY, (1997), vol. 7, no. 2, pages 4 - 733,
    - CRAMERI ET AL., "Construction and evolution of antibody-phage libraries by DNA shuffling", NATURE MEDICINE, (1996), vol. 2, pages 100 - 103,
    - GATES ET AL., "Affnity selective isolation of ligands from peptide libraries through display on a lac repressor 'headpiece dimer", JOURNAL OF MOLECULAR BIOLOGY, (1996), vol. 255, pages 373 - 386,
    - "Sexual PCR and Assembly PCR", STEMMER, The Encyclopedia of Molecular Biology, VCH PUBLISHERS, (1996), pages 447 - 457,
    - CRAMERI, STEMMER, "Combinatorial multiple cassette mutagenesis creates all the permutations of mutant and wildtype cassettes", BIOTECHNIQUES, (1995), vol. 18, pages 194 - 19,
    - STEMMER ET AL., "Single-step assembly of a gene and entire plasmid form large numbers of oligodeoxyribonucleotides", GENE, (1995), vol. 164, pages 49 - 53,
    - STEMMER, "The Evolution of Molecular Computation", SCIENCE, (1995), vol. 270, page 1510,
    - STEMMER, "Searching Sequence Space", BIO/TECHNOLOGY, (1995), vol. 13, pages 549 - 553,
    - STEMMER, "Rapid evolution of a protein in vitro by DNA shuffling", NATURE, (1994), vol. 370, pages 389 - 391,
    - STEMMER, "DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution", PROC. NATL. ACAD. SCI. USA, (1994), vol. 91, pages 10747 - 10751,
    - LING ET AL., "Approaches to DNA mutagenesis: an overview", ANAL BIOCHEM., (1997), vol. 254, no. 2, pages 157 - 178,
    - DALE ET AL., "Oligonucleotide-directed random mutagenesis using the phosphorothioate method", METHODS MOL. BIOL., (1996), vol. 57, pages 369 - 374,
    - SMITH, "In vitro mutagenesis", ANN. REV. GENET., (1985), vol. 19, pages 423 - 462,
    - BOTSTEIN, SHORTLE, "Strategies and applications of in vitro mutagenesis", SCIENCE, (1985), vol. 229, pages 1193 - 1201,
    - CARTER, "Site-directed metagenesis", BIOCHEM. J., (1986), vol. 237, pages 1 - 7,
    - "The efficiency of oligonucleotide directed mutagenesis", ECKSTEIN, F. AND LILLEY, D. M. J., Nucleic Acids & Molecular Biology, SPRINGER VERLAG, (1987),
    - KUNKEL, "Rapid and efficient site-specific mutagenesis without phenotypic selection", PROC. NATL. ACAD. SCI. USA, (1985), vol. 82, pages 488 - 492,
    - KUNKEL ET AL., "Rapid and efficient site-specific mutagenesis without phenotypic selection", METHODS IN ENZYMOL., (1987), vol. 154, pages 367 - 382,
    - BASS ET AL., "Mutant Trp repressors with new DNA-binding specificities", SCIENCE, (1988), vol. 242, pages 240 - 245,
    - METHODS IN ENZYMOL., (1983), vol. 100, pages 468 - 500,
    - METHODS IN ENZYMOL., vol. 154, pages 329 - 350,
    - ZOLLER, "Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any DNA fragment", NUCLEIC ACIDS RES., (1982), vol. 10, pages 6487 - 6500,
    - ZOLLER, SMITH, "Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors", METHODS IN ENZYMOL., (1983), vol. 100, pages 468 - 500,
    - ZOLLER, "Oligonucleotide-directed mutagenesis: a simple method using two oligonucleotide primers and a single-stranded DNA template", METHODS IN ENZYMOL., (1987), vol. 154, pages 329 - 350,
    - TAYLOR, "The use of phosphorothioate-modified DNA in restriction enzyme reactions to prepare nicked DNA", NUCL. ACIDS RES., (1985), vol. 13, pages 8749 - 8764,
    - TAYLOR, "The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA", NUCL. ACIDS RES., (1985), vol. 13, pages 8765 - 8787,
    - NAKAMAYE, "Inhibition of restriction endonuclease Nci I cleavage by phosphorothioate groups and its application to oligonucleotide-directed mutagenesis", NUCL. ACIDS RES., (1986), vol. 14, pages 9679 - 9698,
    - SAYERS, "Y-T Exonucleases in phosphorothioate-based oligonucleotide-directed mutagenesis", NUCL. ACIDS RES., (1988), vol. 16, pages 791 - 802,
    - SAYERS ET AL., "Strand specific cleavage of phosphorothioate- containing DNA by reaction with restriction endonucleases in the presence of ethidium bromide", NUCL. ACIDS RES., (1988), vol. 16, pages 803 - 814,
    - KRAMER ET AL., "The gapped duplex DNA approach to oligonucleotide-directed mutation construction", NACL. ACIDS RES., (1984), vol. 12, pages 9441 - 9456,
    - KRAMER, FRITZ, "Oligonucleotide-directed construction of mutations via gapped duplex DNA", METHODS IN ENZYMOL., (1987), vol. 154, pages 350 - 367,
    - KRAMER, "Improved enzymatic in vitro reactions in the gapped duplex DNA approach to oligonucleotide-directed construction of mutations", NUCL. ACIDS RES., (1988), vol. 16, page 7207,
    - FRITZ, "Oligonucleotide-directed construction of mutations: a gapped duplex procedure without enzymatic reactions in vitro", NUCL. ACIDS RES., (1988), vol. 16, pages 6987 - 6999,
    - KRAMER, "Point Mismatch Repair", CELL, (1984), vol. 38, pages 879 - 887,
    - CARTER ET AL., "Improved oligonucleotide site-directed mutagenesis using M13 vectors", NUCL. ACIDS RES., (1985), vol. 13, pages 4431 - 4443,
    - CARTER, "Improved oligonucleotide-directed mutagenesis using M13 vectors", METHODS IN ENZYMOL., (1987), vol. 154, pages 382 - 403,
    - EGHTEDARZADEH, "Use of oligonucleotides to generate large deletions", NUCL. ACIDS RES., (1986), vol. 14, page 5115,
    - WELLS ET AL., "Importance of hydrogen-bond formation in stabilizing the transition state of subtilisin", PHIL. TRANS. R. SOC. LOND. A, (1986), vol. 317, pages 415 - 423,
    - NAMBIAR ET AL., "Total synthesis and cloning of a gene coding for the ribonuclease S protem", SCIENCE, (1984), vol. 223, pages 1299 - 1301,
    - NUCL. ACIDS RES., vol. 14, pages 6361 - 6372,
    - WELLS ET AL., "Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites", GENE, vol. 34, pages 315 - 323,
    - GRUNDSTROM ET AL., "Oligonucleotide-directed mutagenesis by microscale ''shot-gun'' gene synthesis", NUCL. ACIDS RES., (1985), vol. 13, pages 3305 - 3316,
    - ARNOLD, "Protein engineering for unusual environments", CURRENT OPIMON IN BIOTECHNOLOGY, (1993), vol. 4, pages 450 - 455,
    - "Oligonucleotide-directed double-strand break repair in plasmids of Escherichia coli: a method for site-specific mutagenesis", PROC. NATL. ACAD. SCI. USA, vol. 83, pages 7177 - 7181,
    - METHODS IN ENZYMOLOGY, vol. 154,
    - LEUNG, D.W. ET AL., TECHNIQUE, (1989), vol. 1, pages 11 - 15,
    - CALDWELL, R. C., JOYCE G.F., PCR METHODS APPLIC., (1992), vol. 2, pages 28 - 33,
    - REIDHAAR-OLSON, SCIENCE, (1988), vol. 241, pages 53 - 57,
    - STEMMER, PROC. NATL. ACAD. SCI. USA, (1994), vol. 91, pages 10747 - 10751,
    - ARKIN, A.P., YOUVAN, D.C., PNAS, USA, (1992), vol. 89, pages 7811 - 7815,
    - DELEGRAVE, S., YOUVAN, D.C., BIOTECHNOLOGY RESEARCH, (1993), vol. 11, pages 1548 - 1552,
    - ARNOLD, F.H., CURRENT OPINION IN BIOTECHNOLOGY, (1993), vol. 4, pages 450 - 455,
    - BACA, INT. J. PARASITOL., (2000), vol. 30, pages 113 - 118,
    - HALE, PROTEIN EXPR. PURIF., (1998), vol. 12, pages 185 - 188,
    - NARUM, INFECT. IMMUN., (2001), vol. 69, pages 7250 - 7253,
    - OUTCHKOUROV, PROTEIN EXPR. PURIF., (2002), vol. 24, pages 18 - 24,
    - FENG, BIOCHEMISTRY, (2000), vol. 39, pages 15399 - 15409,
    - HUMPHREYS, PROTEIN EXPR. PURIF., (2000), vol. 20, pages 252 - 264,
    - GAO, BIOTECHNOL PROG., (2004), vol. 20, pages 443 - 448,
    - STREPP, PROC NATL. ACAD. SCI. USA, (1998), vol. 95, pages 4368 - 4373,
    - MIAO, PLANT J, (1995), vol. 7, pages 359 - 365,
    - CHRISTOU, PLANT MOL. BIOL., (1997), vol. 35, pages 197 - 203,
    - PAWLOWSKI, MOL. BIOTECHNOL., (1996), vol. 6, pages 17 - 30,
    - KLEIN, NATURE, (1987), vol. 327, pages 70 - 73,
    - TAKUMI, GENES GENET. SYST., (1997), vol. 72, pages 63 - 69,
    - ROUWENDAL, PLANT MOL. BIOL., (1997), vol. 33, pages 989 - 999,
    - PORTA, "Use of viral replicons for the expression of genes in plants", MOL. BIOTECHNOL., (1996), vol. 5, pages 209 - 221,
    - HORSCH, SCIENCE, (1984), vol. 233, pages 496 - 498,
    - FRALEY, PROC. NATL. ACAD. SCI. USA, (1983), vol. 80, page 4803,
    - POTRYKUS,, Gene Transfer to Plants, Potrykus, SPRINGER.-VERLAG, (1995),
    - HIEI, PLANT MOL. BIOL., (1997), vol. 35, pages 203 - 218,
    - HORSCH, SCIENCE, (1984), vol. 233, page 496,
    - FRALEY, PROC. NATL. ACAD. SCI USA, (1983), vol. 80, page 4803,
    - PARK, PLANT MOL. BIOL., (1996), vol. 32, pages 1135 - 1148,
    - "Protoplasts Isolation and Culture", EVANS ET AL., Handbook of Plant Cell Culture, MACMILLILAN PUBLISHING COMPANY, (1983), pages 124 - 176,
    - Binding, Regeneration of Plants, Plant Protoplasts, CRC PRESS, (1985), pages 21 - 73,
    - KLEE, ANN. REV. OF PLANT PHYS., (1987), vol. 38, pages 467 - 486,
    - PALMGREN, TRENDS GENET., (1997), vol. 13, page 348,
    - CHONG, TRANSGENIC RES., (1997), vol. 6, pages 289 - 296,
    - HENRISSAT, BIOCHEM,. J., (1991), vol. 280, pages 309 - 316,
    - HENRISSAT, BIOCHEM. J., (1993), vol. 293, pages 781 - 788,
    - HENRISSAT, BIOCHEM. J., (1996), vol. 316, pages 695 - 696,
    - HENRISSAT, PLANT PHYSIOLOGY, (2000), vol. 124, pages 1515 - 1519,
    - VAN DE VEN, CRIT.REV. ONCOG., (1993), vol. 4, no. 2, pages 115 - 136,
    - CARUTHERS, NUCLEIC ACIDS RES. SYMP. SER., (1980), pages 215 - 223,
    - HORN, NUCLEIC ACIDS RES. SYMP. SER., (1980), pages 225 - 232,
    - BANGA, A.K., Therapeutic Peptides and Proteins, Formulation, Processing and Delivery Systems, TECHNOMIC PUBLISHING CO., (1995),
    - ROBORGE, SCIENCE, (1995), vol. 269, page 202,
    - MERRIFIELD, METHODS ENZYMOL., (1997), vol. 289, pages 3 - 13,
    - "Peptide Backbone Modifications", SPATOLA, Chemistry and Biochemistry of Amino Acids, Peptides and Proteins, MARCELL DEKKER, (1983), vol. 7, pages 267 - 357,
    - CREIGHTON, T.E., Proteins - Structure and Molecular Properties 2nd Ed.,, W.H. FREEMAN AND COMPANY, (1993),
    - STEWART, J. M., YOUNG, J. D., Solid Phase Peptide Synthesis, 2nd Ed.,, PIERCE CHEMICAL CO., pages 11 - 12,
    - H. M. GEYSEN ET AL., PROC. NATL. ACAD. SCI., USA, (1984), vol. 81, page 3998,
    - Molecular Cloning: A Laboratory Manual, 2nd Ed.,, COLD SPRING HARBOR LABORATORY PRESS, (1989),
    - NIELSEN, "Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites", PROTEIN ENGINEERING, (1997), vol. 10, no. 1, pages 1 - 6,
    - JOHNSTON, CURR. BIOL., (1998), vol. 8, pages R171 - R174,
    - SCHUMMER, BIOTECHNIQUES, (1997), vol. 23, pages 1087 - 1092,
    - SOLINAS-TOLDO, GENES, CHROMOSOMES & CANCER, (1997), vol. 20, pages 399 - 407,
    - BOWTELL, NATURE GENETICS SUPP., (1999), vol. 21, pages 25 - 32,
    - COLIGAN, CURRENT PROTOCOLS IN IMMUNOLOGY, WILEY/GREENE, (1991),
    - STITES, BASIC AND CLINICAL IMMUNOLOGY (7th ed.), LANGE MEDICAL PUBLICATIONS,
    - GODING, MONOCLONAL ANTIBODIES; PRINCIPLES AND PRACTICE(2d ed.), ACADEMIC PRESS, (1986),
    - KOHLER, NATURE, (1975),
    - HARLOW, ANTIBODIES, A LABORATORY MANUAL, COLD SPRING HARBOR PUBLICATIONS, (1988),
    - HOOGENBOOM, TRENDS BIOTECHNOL., (1997), vol. 15, pages 62 - 70,
    - KATZ, ANNU. REV. BIOPHYS. BIOMOL. STRUCT., (1997), vol. 26, pages 27 - 45,
    - KOHLER, MILSTEIN, NATURE, (1975), vol. 256, pages 495 - 497,
    - KOZBOR ET AL., IMMUNOLOGY TODAY, (1983), vol. 4, page 72,
    - COLE ET AL., Monoclonal Antibodies and Cancer Therapy, ALAN R. LISS, INC., (1985), pages 77 - 96,
    - "Methods for Measuring Cellulase Activities", METHODS IN ENZYMOLOGY, vol. 160, pages 87 - 116,
    - KREUZER, BR. J. HAEMATOL., (2001), vol. 114, pages 313 - 318,
    - XIA, TRANSPLANTATION, (2001), vol. 72, pages 907 - 914,
    - HOONDAL, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, (2002), vol. 59, pages 409 - 418,
    - BEDFORD ET AL., PROCEEDINGS OF THE 1ST SYMPOSIUM ON ENZYMES IN ANIMAL NUTRITION, (1993), pages 73 - 77,
    - KUNG, L. ET AL., J. DAIRY SCIENE, (200001), vol. 83, pages 115 - 122,
    - MEULEN, J. ET AL., ARCH. TIERERNAHR, (2001), vol. 54, pages 101 - 115,
    - JARONI, D. ET AL., POULT. SCI., (19990607), vol. 8, pages 841 - 847,
 US19990407800
 WO2000US01202
 US20000618579
 WO2000US01138
 US20000656549
 US19990332835
 WO1994US09174