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Extract from the Register of European Patents

EP About this file: EP3686272

EP3686272 - ORGANISMS FOR THE PRODUCTION OF 1,3-BUTANEDIOL [Right-click to bookmark this link]
StatusThe application is deemed to be withdrawn
Status updated on  04.06.2021
Database last updated on 23.04.2024
FormerThe application has been published
Status updated on  26.06.2020
Most recent event   Tooltip04.06.2021Application deemed to be withdrawnpublished on 07.07.2021  [2021/27]
Applicant(s)For all designated states
Genomatica, Inc.
4757 Nexus Center Drive
San Diego, CA 92121 / US
[2020/31]
Inventor(s)01 / Burgard, Anthony P.
10520 Waterridge Circle
San Diego, CA 92121 / US
02 / Burk, Mark J.
10520 Waterridge Circle
San Diego, CA 92121 / US
03 / Osterhout, Robin E.
10520 Waterridge Circle
San Diego, CA 92121 / US
04 / Pharkya, Priti
10520 Waterridge Circle
San Diego, CA 92121 / US
 [2020/31]
Representative(s)Hoffmann Eitle
Patent- und Rechtsanwälte PartmbB
Arabellastraße 30
81925 München / DE
[2020/31]
Application number, filing date19212136.630.04.2010
[2020/31]
Priority number, dateUS20090174473P30.04.2009         Original published format: US 174473 P
[2020/31]
Filing languageEN
Procedural languageEN
PublicationType: A1 Application with search report 
No.:EP3686272
Date:29.07.2020
Language:EN
[2020/31]
Search report(s)(Supplementary) European search report - dispatched on:EP09.06.2020
ClassificationIPC:C12N1/21, C12P7/18, C12N9/04
[2020/31]
CPC:
C12P7/18 (EP,CN,KR,US); C12N15/52 (EP,CN,KR,US); C12N9/0006 (EP,CN,KR,US);
C07C29/80 (US); C12N1/00 (KR); C12N1/02 (KR);
C12N1/20 (KR); C12N1/38 (KR); C12N9/0008 (EP,CN,US);
C12N9/1029 (EP,CN,US); C12N9/1096 (EP,CN,US); C12N9/88 (EP,CN,US);
C12P7/16 (KR); C12Y101/0101 (CN); C12Y101/01157 (EP,CN,KR,US);
C12Y102/01 (CN,US); C12Y206/01 (CN,US); C12Y401/01 (CN,US);
C12Y402/01 (CN,US); C12Y403/01 (CN,US); C12Y101/01 (US);
C12Y203/01 (US); Y02E50/10 (EP); Y02P20/52 (KR) (-)
Designated contracting statesAT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   LV,   MC,   MK,   MT,   NL,   NO,   PL,   PT,   RO,   SE,   SI,   SK,   SM,   TR [2020/31]
TitleGerman:ORGANISMEN ZUR HERSTELLUNG VON 1,3-BUTANDIOL[2020/31]
English:ORGANISMS FOR THE PRODUCTION OF 1,3-BUTANEDIOL[2020/31]
French:ORGANISMES DE PRODUCTION DE 1,3-BUTANEDIOL[2020/31]
Examination procedure30.01.2021Application deemed to be withdrawn, date of legal effect  [2021/27]
19.02.2021Despatch of communication that the application is deemed to be withdrawn, reason: examination fee not paid in time  [2021/27]
Parent application(s)   TooltipEP10770464.5  / EP2424975
EP16168278.6  / EP3135760
EP17188529.6  / EP3318626
Divisional application(s)EP21153963.0  / EP3865569
EP23201197.3
Fees paidRenewal fee
27.12.2019Renewal fee patent year 03
27.12.2019Renewal fee patent year 04
27.12.2019Renewal fee patent year 05
27.12.2019Renewal fee patent year 06
27.12.2019Renewal fee patent year 07
27.12.2019Renewal fee patent year 08
27.12.2019Renewal fee patent year 09
27.12.2019Renewal fee patent year 10
17.04.2020Renewal fee patent year 11
15.04.2021Renewal fee patent year 12
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Documents cited:Search[XY]US2003032152  (PORRO DANILO [IT], et al) [X] 1-10 * the whole document * * paragraph [0001] * * claims 1, 12, 13 * [Y] 11-15;
 [XY]WO2008080124  (GEVO [US], et al) [X] 1-10 * the whole document * * page 55; example 29 * [Y] 1-15;
 [Y]  - MATSUYAMA A ET AL, "Industrial production of (R)-1,3-butanediol by new biocatalysts", JOURNAL OF MOLECULAR CATALYSIS. B, ENZYMATIC, ELSEVIER, AMSTERDAM, NL, vol. 11, no. 4-6, ISSN 1381-1177, (20010122), pages 513 - 521, (20010122), XP027407301 [Y] 1-15 * the whole document * * abstract * * tables 1, 2 *
 [XA]  - YUAN-ZHENG QIU ET AL, "Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from Gluconate and Glucose by Recombinant Aeromonas hydrophila and Pseudomonas putida", BIOTECHNOLOGY LETTERS, SPRINGER NETHERLANDS, DORDRECHT, (20050901), vol. 27, no. 18, doi:10.1007/S10529-005-3685-6, ISSN 1573-6776, pages 1381 - 1386, XP019230960 [X] 1-10 * the whole document * * abstract * [A] 11-15

DOI:   http://dx.doi.org/10.1007/s10529-005-3685-6
 [T]  - Naoya Kataoka ET AL, "Improvement of (R)-1,3-butanediol production by engineered Escherichia coli", Journal of bioscience and bioengineering, Japan, doi:10.1016/j.jbiosc.2012.11.025, (20130104), pages 475 - 480, URL: http://www.ncbi.nlm.nih.gov/pubmed/23290993, (20131119), XP055089100 [T] 1-15 * the whole document * * figure 1 *

DOI:   http://dx.doi.org/10.1016/j.jbiosc.2012.11.025
 [T]  - YUDONG JIANG ET AL, "Microbial production of short chain diols", MICROBIAL CELL FACTORIES, BIOMED CENTRAL, GB, (20141210), vol. 13, no. 1, doi:10.1186/S12934-014-0165-5, ISSN 1475-2859, page 165, XP021207510 [T] 1-15 * the whole document *

DOI:   http://dx.doi.org/10.1186/s12934-014-0165-5
by applicantUS2002012939
 WO02055995
 US2002168654
 US2003059792
 US2003224363
 US2003233218
 WO03106998
 US2004009466
 US2004029149
 US2004072723
 US2005221466
 US7127379
 WO2008115840
 WO2009014437
 US2009047719
 US2009075351
    - ICHIKAWA et al., J. of Molecular Catalysis A-Chemical, (20060000), vol. 256, pages 106 - 112
    - ICHIKAWA et al., J. of Molecular Catalysis A-Chemical, (20050000), vol. 231, pages 181 - 189
    - TANI et al., Appl. Environ. Microbiol., (20000000), vol. 66, pages 5231 - 5235
    - ATSUMI et al., Nature, (20080000), vol. 451, pages 86 - 89
    - SULZENBACHER et al., J. of Molecular Biology, (20040000), vol. 342, pages 489 - 502
    - WALTER et al., J. of Bacteriology, (19920000), vol. 174, pages 7149 - 7158
    - PEREZ et al., J. Biol. Chem., (20080000), vol. 283, pages 7346 - 7353
    - KINOSHITA et al., Appl. Microbiol. Biotechnol, (19850000), vol. 22, pages 249 - 254
    - BRAVO et al., J. Forensic Sci., (20040000), vol. 49, pages 379 - 387
    - WOLFF et al., Protein Expr. Purif., (19950000), vol. 6, pages 206 - 212
    - BREITKREUZ et al., J. Biol. Chem., (20030000), vol. 278, pages 18588 - 18596
    - JEON et al., J. Biotechnol., (20080000), vol. 135, pages 127 - 133
    - LOKANATH et al., J. Mol. Biol., (20050000), vol. 352, pages 905 - 917
    - MANNING et al., Biochem J., (19850000), vol. 231, pages 481 - 484
    - HAWES et al., Methods Enzymol, (20000000), vol. 324, pages 218 - 228
    - CHOWDHURY et al., Biosci. Biotechnol Biochem., (19960000), vol. 60, pages 2043 - 2047
    - ABERHART et al., J. Chem. Soc., (19790000), vol. 6, pages 1404 - 1406
    - CHOWDHURY et al., Biosci. Biotechnol Biochem., (20030000), vol. 67, pages 438 - 441
    - STAMES et al., Biochemistry, (19730000), vol. 11, pages 677 - 687
    - SIBILLI et al., J. Biol. Chem., (19810000), vol. 256, pages 10228 - 10230
    - JAMES et al., Biochemistry, (20020000), vol. 41, pages 3720 - 3725
    - CAHYANTO et al., Microbiology, (20060000), vol. 152, pages 205 - 112
    - JACQUES et al., Biochem. Biophys. Acta, (20010000), vol. 1544, pages 28 - 41
    - JONES et al., Microbiol. Rev., (19860000), vol. 50, pages 484 - 524
    - YOUNGLESON et al., J. Bacteriol., (19890000), vol. 171, pages 6800 - 6807
    - BINSTOCK et al., Methods Enzymol., (19810000), vol. 71C, pages 403 - 411
    - PLOUX et al., Eur. J. Biochem., (19880000), vol. 174, pages 177 - 182
    - ALBER et al., Mol. Microbiol., (20060000), vol. 61, pages 297 - 309
    - PEOPLES et al., Mol. Microbiol., (19890000), vol. 3, pages 349 - 357
    - HILLMERGOTTSCHALK, Biochim. Biophys. Acta, (19740000), vol. 3334, pages 12 - 23
    - WAKIL et al., J. Biol. Chem., (19540000), vol. 207, pages 631 - 63 8
    - BERG et al., Archaea. Science, (20070000), vol. 318, pages 1782 - 1786
    - ISMAIEL et al., J. Bacteriol, (19930000), vol. 175, pages 5097 - 5105
    - LAMED et al., Biochem. J., (19810000), vol. 195, pages 183 - 190
    - PERETZ et al., Biochemistry, (19890000), vol. 28, pages 6549 - 6555
    - KILLENBERG-JABS et al., Eur. J. Biochem., (20010000), vol. 268, pages 1698 - 1704
    - EDEGGER et al., Chem. Commun. (Camb), (20060000), pages 2402 - 2404
    - KOSJEK et al., Biotechnol. Bioeng., (20040000), vol. 86, pages 55 - 62
    - STEINBUCHEL et al., Eur. J. Biochem., (19830000), vol. 130, pages 329 - 334
    - SUDA et al., Arch. Biochem. Biophys., (19760000), vol. 176, pages 610 - 620
    - SUDA et al., Biochem. Biophys. Res. Commun., (19770000), vol. 77, pages 586 - 591
    - MARKS et al., J. BioJ. Chem., (19920000), vol. 267, pages 15459 - 15463
    - KESSLER et al., FEBS. Lett., (19910000), vol. 281, pages 59 - 63
    - FONTAINE et al., J. Bacteriol., (20020000), vol. 184, pages 2404 - 2410
    - KAZAHAYA et al., J. Gen. Appl. Microbiol., (19720000), vol. 18, pages 43 - 55
    - KOO et al., Biotechnol. Lett., (20050000), vol. 27, pages 505 - 510
    - STRAUSS et al., Eur. J. Biochem., (19930000), vol. 215, pages 421 - 429
    - KLATT et al., Environ. Microbiol., (20070000), vol. 9, pages 2067 - 2078
    - METZ et al., Plant Physiology, (20000000), vol. 122, pages 635 - 644
    - REISER et al., J. of Bacteriology, (19970000), vol. 179, pages 2969 - 2975
    - ISHIGE et al., Appl. Environ. MiavbioL, (20020000), vol. 68, pages 1192 - 1195
    - BOYNTON et al., J. Bacteriol., (19960000), vol. 178, pages 3015 - 3024
    - TAKAHASHI et al., J. Bacteriol, (20000000), vol. 182, pages 4704 - 4710
    - POWLOWSKI et al., J. Bacteriol., (19930000), vol. 175, pages 377 - 385
    - KOSAKA et al., Biosci. Biotechnol. Biochem., (20070000), vol. 71, pages 58 - 61
    - THAUER, R.K., Science, (20070000), vol. 318, pages 1732 - 1733
    - ALBER et al., J. BacterioL, (20060000), vol. 188, pages 8551 - 8559
    - TOTH et al., Appl. Environ. Microbiol, (19990000), vol. 65, pages 4973 - 4980
    - MCPHERSON et al., Nucleic.Acids Res., (19830000), vol. 11, pages 5257 - 5266
    - KORBER et al., J.Mol.Biol., (19930000), vol. 234, pages 1270 - 1273
    - KORT et al., Extremophiles, (19970000), vol. 1, pages 52 - 60
    - LEBBINK et al., J.MolBiol., (19980000), vol. 280, pages 287 - 296
    - LEBBINK et al., J.Mol.Biol., (19990000), vol. 289, pages 357 - 369
    - INGOLDSBY et al., Gene, (20050000), vol. 349, pages 237 - 244
    - KHAN et al., Biosci.Biotechnol Biochem., (20050000), vol. 69, pages 1861 - 1870
    - PURNELL et al., Planta, (20050000), vol. 222, pages 167 - 180
    - ABIKO et al., Plant Cell Physiol, (20050000), vol. 46, pages 1724 - 1734
    - DIAZ et al., Extremophiles, (20060000), vol. 10, pages 105 - 115
    - HAYDEN et al., FEMS Microbiol Lett., (20020000), vol. 211, pages 37 - 41
    - ROCA et al., Appl Environ.Microbiol, (20030000), vol. 69, pages 4732 - 4736
    - STOYAN et al., JBioteehnol, (19970000), vol. 54, pages 77 - 80
    - ANSORGE et al., BiotechnolBioeng, (20000000), vol. 68, pages 557 - 562
    - YANG et al., J.Biol.Chem., (20030000), vol. 278, pages 8804 - 8808
    - KREIMEYER et al., JBiolChem., (20070000), vol. 282, pages 7191 - 7197
    - BAKER et al., JBiol.Chem., (19720000), vol. 247, pages 7724 - 7734
    - BAKER et al., Biochemistry, (19740000), vol. 13, pages 292 - 299
    - MISONO et al., JBacteriol., (19820000), vol. 150, pages 398 - 401
    - HEYDARI et al., Appl Environ.Microbiol, (20040000), vol. 70, pages 937 - 942
    - HASHIMOTO et al., JBiochem., (19890000), vol. 106, pages 76 - 80
    - MISONONAGASAKI, J Bacteri ., (19820000), vol. 150, pages 398 - 401
    - RULDEEKULTHAMRONG et al., BMB.Rep., (20080000), vol. 41, pages 790 - 795
    - JENG et al., A. Biochemistry, (19740000), vol. 13, pages 2898 - 2903
    - KENKLIES et al., Microbiology, (19990000), vol. 145, pages 819 - 826
    - YAGI et al., FEBS Lett., (19790000), vol. 100, pages 81 - 84
    - YAGI et al., Methods Enzymol., (19850000), vol. 133, pages 83 - 89
    - YAGI et al., J. Biochem., (19820000), vol. 92, pages 35 - 43
    - KWOK et al., J. Exp. Bot., (20040000), vol. 55, pages 595 - 604
    - DE LA et al., Plant J., (20060000), vol. 46, pages 414 - 425
    - WILKIE et al., Protein Expr. Purif., (19980000), vol. 12, pages 381 - 389
    - RECASENS et al., Biochemistry, (19800000), vol. 19, pages 4583 - 4589
    - WHALEN et al., J. Bacteriol., (19820000), vol. 150, pages 739 - 746
    - HUGHES et al., J. Bacteriol., (19840000), vol. 158, pages 571 - 574
    - MIYAZAKI et al., Microbiol., (20040000), vol. 150, pages 2327 - 2334
    - OKUNO et al., Enz. Prot., (19930000), vol. 47, pages 136 - 148
    - TANIZAWA et al., J. Biol. Chem., (19890000), vol. 264, pages 2450 - 2454
    - PEISACH et al., Biochemistry, (19980000), vol. 37, pages 9918 - 9930
    - GUTIERREZ et al., Eur. J. Biochem, (20000000), vol. 267, pages 7218 - 7223
    - GUTIERREZ et al., Protein Eng., (19980000), vol. 11, pages 53 - 58
    - TAYLOR et al., Biochim. Biophys. Acta, (19970000), vol. 1350, pages 38 - 40
    - BONNARME et al., J. Bacteriol., (19950000), vol. 177, pages 3573 - 3578
    - MARTINEZ-CARRION et al., J. Biol. Chem., (19650000), vol. 240, pages 3538 - 3546
    - CHANDRA et al., ARch. Microbiol., (20010000), vol. 176, pages 443 - 451
    - ICHIKAWA et al., J. Mol. Catalysis A-Chem., (20060000), vol. 256, pages 106 - 112
    - CHOPRA et al., Protein Expr. Purif., (20020000), vol. 25, pages 533 - 540
    - KUZNETSOVA et al., FEMSMicrobiol. Rev., (20050000), vol. 29, pages 263 - 279
    - KANAMASA et al., Appl. Microbiol Biotechnol., (20080000), vol. 80, pages 223 - 229
    - DRUMMOND et al., J. Biol. Chem., (19600000), vol. 235, pages 318 - 325
    - HAMMER et al., J Basic Microbiol, (19920000), vol. 32, pages 21 - 27
    - FUJII et al., JBiochem., (20000000), vol. 128, pages 391 - 397
    - ROMERO et al., J nd.Microbiol Biotechnol, (19970000), vol. 18, pages 241 - 246
    - TOBIN et al., J Bacteriol., (19910000), vol. 173, pages 6223 - 6229
    - SODA et al., Biochemistry, (19680000), vol. 7, pages 4110 - 4119
    - IKAI et al., J Bacteriol, (19970000), vol. 179, pages 5118 - 5125
    - LU et al., J Bacteriol, (20020000), vol. 184, pages 3765 - 3773
    - SAMSONOVA et al., BMC.Microbiol, (20030000), vol. 3, page 2
    - KIM, JBiolChem., (19640000), vol. 239, pages 783 - 786
    - LI et al., Biochemistry, (19990000), vol. 38, pages 10004 - 10012
    - SCHURE et al., Appl. Environ. Microbiol., (19980000), vol. 64, pages 1303 - 1307
    - SIEGERT et al., Protein Eng. Des. Sel., (20050000), vol. 18, pages 345 - 357
    - CHANDRA et al., Arch. Microbiol., (20010000), vol. 176, pages 443 - 451
    - KRIEGER et al., Eur. J. Biochem., (20020000), vol. 269, pages 3256 - 3263
    - POLOVNIKOVA et al., Biochemistry, (20030000), vol. 42, pages 1820 - 1830
    - LINGEN et al., Chembiochem, (20030000), vol. 4, pages 721 - 726
    - LINGEN et al., Protein Eng., (20020000), vol. 15, pages 585 - 593
    - BARROWMAN et al., FEMS Microbiology Letters, (19860000), vol. 34, pages 57 - 60
    - HENNING et al., Appl. Environ. Microbiol., (20060000), vol. 72, pages 7510 - 7517
    - TIAN et al., Proc. Natl. Acad. Sci. USA, (20050000), vol. 102, pages 10670 - 10675
    - GREEN et al., J. Bacteriol., (20000000), vol. 182, pages 2838 - 2844
    - SHIGEOKA et al., Arch. Biochem. Biophys., (19910000), vol. 288, pages 22 - 28
    - OKU et al., J Biol. Chem., (19880000), vol. 263, pages 18386 - 18396
    - SMIT et al., Appl. Environ. Microbiol., (20050000), vol. 71, pages 303 - 311
    - BERTHOLD et al., D. Biol. Crystallogr., (20070000), vol. 63, pages 1217 - 1224
    - MOMANY et al., D. BioL Crystallogr., (20020000), vol. 58, pages 549 - 552
    - KEFALA et al., Acta. Crystallogr. Sect. F. Struct. Biol. Cryst. Commun., (20050000), vol. 61, pages 782 - 784
    - ANDERSEN et al., Gene, (19930000), vol. 124, pages 105 - 109
    - TSUJIMOTO et al., J. Biotechnol, (20060000), vol. 124, pages 327 - 337
    - HU et al., J. BioL Chem., (20080000), vol. 283, pages 21284 - 21293
    - QU et al., Biochem. J., (20030000), vol. 375, pages 465 - 470
    - FITZGERALD et al., DNA, (19890000), vol. 8, pages 623 - 634
    - DUSCH et al., Appl. Environ. Microbiol., (19990000), vol. 65, pages 1530 - 1539
    - RAMJEE et al., Biochem. J., (19970000), vol. 323, pages 661 - 669
    - MERKEL et al., FEMS Microbiol. Lett., (19960000), vol. 143, pages 247 - 252
    - SCHMITZBERGER et al., EMBOJ., (20030000), vol. 22, pages 6193 - 6204
    - TANAKA et al., J. Appl. Microbiol., (20080000), vol. 104, pages 1283 - 1293
    - TAKATSUKA et al., Biosci. Biotechnol Biochem., (19990000), vol. 63, pages 1843 - 1846
    - LEE et al., Biochem. J., (20010000), vol. 360, pages 657 - 665
    - GUIRARD et al., JBiol.Chem., (19800000), vol. 255, pages 5960 - 5964
    - LEE et al., JBiol. Chem., (20070000), vol. 282, pages 27115 - 27125
    - WILLKE et al., Appl. Microbiol. Biotechnol., (20010000), vol. 56, pages 289 - 295
    - KANAMASA et al., Appl. Microbiol. BiotechnoL, (20080000), vol. 80, pages 223 - 229
    - SHINGLER et al., J. Bacteriol., (19920000), vol. 174, pages 711 - 724
    - KATO et al., Arch. Microbiol., (19970000), vol. 168, pages 457 - 463
    - IFFLAND et al., Biochemistry, (20000000), vol. 39, pages 10790 - 10798
    - LIAN et al., J. Am. Chem. Soc., (19940000), vol. 116, pages 10403 - 10411
    - CLAUSEN et al., Gene, (19940000), vol. 142, pages 107 - 112
    - BARTHELMEBS et al., Appl. Environ. Microbiol., (20010000), vol. 67, pages 1063 - 1069
    - RODRIGUEZ et al., J. Agric. Food Chem., (20080000), vol. 56, pages 3068 - 3072
    - QI et al., Biochem. J., (20070000), vol. 375, pages 465 - 470
    - UCHIYAMA et al., Biosci. Biotechnol. Biochem., (20080000), vol. 72, pages 116 - 123
    - HASHIDOKO et al., Biosci. Biotech. Biochem., (19940000), vol. 58, pages 217 - 218
    - CAVIN et al., Appl. Environ. Microbiol.., (19980000), vol. 64, pages 1466 - 1471
    - HUANG et al., J. Bacteriol., (19940000), vol. 176, pages 5912 - 5918
    - SARIASLANI, F.S., Annu. Rev. Microbiol., (20070000), vol. 61, pages 51 - 69
    - BENNER et al., J. Am. Chem. So., (19810000), vol. 103, pages 993 - 994
    - HIGHBARGER et al., Biochemistry, (19960000), vol. 35, pages 41 - 46
    - PETERSEN et al., Appl. Environ. Microbiol., (19900000), vol. 56, pages 3491 - 3498
    - ROZZEL et al., J. Am. Chem. Soc., (19840000), vol. 106, pages 4937 - 4941
    - KOSAKA et al., Biosci.Biotechnol Biochem., (20070000), vol. 71, pages 58 - 68
    - RAVAGNANI et al., Mol. Microbiol., (20000000), vol. 37, pages 1172 - 1185
    - MATIASEK et al., Curr. Microbiol., (20010000), vol. 42, pages 276 - 281
    - KALAPOS, Biochim. Biophys. Acta, (20030000), vol. 1621, pages 122 - 139
    - WEAVER, T., B. Biol. Crystallogr., (20050000), vol. 61, pages 1395 - 1401
    - TSENG et al., J Bacteriol, (20010000), vol. 183, pages 461 - 467
    - WOODS et al., Biochem. Biophys. Acta., (19880000), vol. 954, pages 14 - 26
    - GUEST et al., J. Gen. Microbiol., (19850000), vol. 131, pages 2971 - 2984
    - SMITH et al., Int. J. Biochem. Cell Bioi., (19990000), vol. 31, pages 961 - 975
    - MIZOBATA et al., Arch. Biochem. Biophys., (19980000), vol. 355, pages 49 - 55
    - KOBAYASHI et al., J. Biochem., (19810000), vol. 89, pages 1923 - 1931
    - SHIMOYAMA et al., FEMS Microbiol. Lett, (20070000), vol. 270, pages 207 - 213
    - ALHAPEL et al., Proc. Natl. Acad. Sci. USA, (20060000), vol. 103, pages 12341 - 12346
    - KOLLMANN-KOCH et al., Physiol. Chem., (19840000), vol. 365, pages 847 - 857
    - WANG et al., J. Biol. Chem., (19690000), vol. 244, pages 2516 - 2526
    - HILLMER et al., FEBS Lett., (19720000), vol. 21, pages 351 - 354
    - ROBERTS et al., Arch. Microbiol., (19780000), vol. 117, pages 99 - 108
    - AGNIHOTRI et al., Bioorg. Med. Chem., (20030000), vol. 11, pages 9 - 20
    - CONRAD et al., J. Bacteriol., (19740000), vol. 118, pages 103 - 111
    - OLIVERA et al., Proc. Natl. Acad. Sci USA, (19980000), vol. 95, pages 6419 - 6424
    - PARK et al., J Bacteriol., (20030000), vol. 185, pages 5391 - 5397
    - ISMAIL et al., Eur. J. Biochem., (20030000), vol. 270, pages 3047 - 3054
    - PARK et al., Appl. Biochem. Biotechnol., (20040000), vol. 113-116, pages 335 - 346
    - PARK et al., Biotechnol Bioeng., (20040000), vol. 86, pages 681 - 686
    - MATTHIES et al., Appl. Environ. Micriobiol., (19920000), vol. 58, pages 1435 - 1439
    - SCHERF et al., Arch. Microbiol.,, (19940000), vol. 161, pages 239 - 245
    - MA et al., Ann. N.Y. Acad. Sci., (19920000), vol. 672, pages 60 - 65
    - ASANO et al., Biomol. Eng., (20050000), vol. 22, pages 95 - 101
    - SJOSTROM et al., Biochem. Biophys. Acta., (19970000), vol. 1324, pages 182 - 190
    - TAKAGI et al., J. Biochem., (19840000), vol. 96, pages 545 - 552
    - TAKAGI et al., J. Bacteriol., (19850000), vol. 161, pages 1 - 6
    - ASUNCION et al., JBiol Chem., (20020000), vol. 277, pages 8306 - 8311
    - KATO et al., Arch.Microbiol, (19970000), vol. 168, pages 457 - 463
    - ASANO et al., FEMS Microbiol Lett, (19940000), vol. 118, pages 255 - 258
    - NOWICKI et al., Biochim. Biophys. Acta, (20010000), vol. 1546, pages 268 - 281
    - BREDWELL et al., Biotechnol Prog., (19990000), vol. 15, pages 834 - 844
    - HOFFINEISTER et al., J. Biol. Chem., (20050000), vol. 280, pages 4329 - 4338
    - LIN et al., Biotechnol. Bioeng., (20050000), vol. 90, pages 775 - 779
    - HANAI et al., Appl. Environ. Microbiol., (20070000), vol. 73, pages 7814 - 7818
    - SAKAI et al., JBiosci.Bioeng, (20050000), vol. 99, pages 252 - 258
    - DATAR et al., Biotechnol Bioeng, (20040000), vol. 86, pages 587 - 594
    - NAJAFPOUR et al., Enzyme and Microbial Technology, (20060000), vol. 38, no. 1-2, pages 223 - 228
    - AHMED et al., Biotechnol Bioeng, (20070000), vol. 97, pages 1080 - 1086
    - HIRANO et al., J. Biosci. Bioeng., (20050000), vol. 100, pages 318 - 322
    - PAXTON et al., Biochem. J., (19860000), vol. 234, pages 295 - 303
    - SINCLAIR et al., Biochem. Mol. BioI. Int., (19930000), vol. 31, pages 911 - 922
    - WARD et al., Archaea., (20020000), vol. 1, pages 133 - 141
    - VERNAL et al., FEMS Microbiol. Lett., (20030000), vol. 229, pages 217 - 222
    - VERNAL et al., Mol. Biochem. Parasitol., (19980000), vol. 96, pages 83 - 92
    - REETZ et al., Angew. Chem. Int. Ed Engl., (19970000), vol. 36, pages 2830 - 2832
    - REETZ et al., Angew. Chem. Int. Ed Engl., (20050000), vol. 44, pages 4192 - 4196
    - YANO et al., Proc. Natl. Acad. Sci U. S. A, (19980000), vol. 95, pages 5511 - 5515
    - JIANG et al., Science, (20080000), vol. 319, pages 1387 - 1391
    - COCO et al., Nat. Biotechnol., (20010000), vol. 19, pages 354 - 359
    - HERMES et al., Proc. Natl. Acad. Sci. U. S. A, (19900000), vol. 87, pages 696 - 700
    - FUJITA et al., Biosci. Biotechnol Biochem., (20010000), vol. 65, pages 2695 - 2700
    - JOHNSON et al., Plant J., (20010000), vol. 25, pages 325 - 333
    - DOYLE et al., Biochemistry, (20010000), vol. 40, pages 4234 - 4241
    - CHO et al., Arch. Biochem. Biophys., (20030000), vol. 419, pages 139 - 146
    - ZHANG et al., Proc Natl Acad Sci US. A, (19970000), vol. 94, pages 4504 - 4509
    - ONUFFER et al., Protein Sci., (19950000), vol. 4, pages 1750 - 1757
    - SIEGERT et al., Protein EngDes Sel, (20050000), vol. 18, pages 345 - 357
    - KUMAMARU et al., Nat. Biotechnol, (19980000), vol. 16, pages 663 - 666
    - KINO et al., Appl. Microbiol. Biotechnol., (20070000), vol. 73, pages 1299 - 1305
    - MENG et al., Biochemistry, (19940000), vol. 33, pages 12879 - 12885
    - LIN et al., Biotechnol. Prog., (19990000), vol. 15, page 467471
    - CLARKE et al., Biochem. Biophys. Res. Commun., (19870000), vol. 148, pages 15 - 23
    - WILKS et al., Biochemistry, (19900000), vol. 29, pages 8587 - 8591
    - REIDHAAR-OLSON, J. F.R. T. SAUER, "Combinatorial cassette mutagenesis as a probe of the informational content of protein sequences", Science, (19880000), vol. 241, pages 1541 - 1544
    - HOGAN et al., Biochemistry, (19950000), vol. 34, pages 4225 - 4230
    - WILKS et al., Biochemistry, (19920000), vol. 31, pages 7802 - 7806
    - HIBBERT, E. G.F. BAGANZH. C. HAILESJ. M. WARDG. J. LYEJ. M. WOODLEYP. A. DALBY, "Directed evolution of biocatalytic processes", Biomol.Eng, (20050000), vol. 22, doi:10.1016/j.bioeng.2004.09.003, pages 11 - 19, XP004862541

DOI:   http://dx.doi.org/10.1016/j.bioeng.2004.09.003
    - "Enzyme evolution for chemical process applications", HUISMAN, G. W.J. J. LALONDE, Biocatalysis in the pharmaceutical and biotechnology industries, CRC Press, (20070000), pages 717 - 742
    - OTTEN, L. G.W. J. QUAX, "Directed evolution: selecting today's biocatalysts", Biomol.Eng, (20050000), vol. 22, doi:10.1016/j.bioeng.2005.02.002, pages 1 - 9, XP004862540

DOI:   http://dx.doi.org/10.1016/j.bioeng.2005.02.002
    - SEN, S.D. VENKATA, VB. MANDAL, "Developments in directed evolution for improving enzyme functions", Appl Biochem.Biotechnol, (20070000), vol. 143, pages 212 - 223
    - PRITCHARD, L.D. COMED. KELLJ. ROWLANDM. WINSON, "A general model of error-prone PCR", J Theor.Biol, (20050000), vol. 234, doi:10.1016/j.jtbi.2004.12.005, pages 497 - 509, XP004820145

DOI:   http://dx.doi.org/10.1016/j.jtbi.2004.12.005
    - FUJII, R.M. KITAOKAK. HAYASHI, "One-step random mutagenesis by error-prone rolling circle amplification", Nucleic Acids Res, (20040000), vol. 32, page el45
    - FUJII, R.M. KITAOKAK. HAYASHI, "Error-prone rolling circle amplification: the simplest random mutagenesis protocol", NatProtoc., (20060000), vol. 1, doi:10.1038/nprot.2006.403, pages 2493 - 2497, XP001537727

DOI:   http://dx.doi.org/10.1038/nprot.2006.403
    - STEMMER, W. P., "DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution", Proc Natl Acad Sci U S.A, (19940000), vol. 91, doi:10.1073/pnas.91.22.10747, pages 10747 - 10751, XP002912215

DOI:   http://dx.doi.org/10.1073/pnas.91.22.10747
    - STEMMER, W. P., "Rapid evolution of a protein in vitro by DNA shuffling", Nature, (19940000), vol. 370, doi:10.1038/370389a0, pages 389 - 391, XP002082182

DOI:   http://dx.doi.org/10.1038/370389a0
    - ZHAO, H.L. GIVERZ. SHAOJ. A. AFTHOLTERF. H. ARNOLD, "Molecular evolution by staggered extension process (StEP) in vitro recombination", Nat.Biotechnol, (19980000), vol. 16, doi:10.1038/nbt0398-258, pages 258 - 261, XP000775867

DOI:   http://dx.doi.org/10.1038/nbt0398-258
    - SHAO, Z.H. ZHAOL. GIVERF. H. ARNOLD, "Random-priming in vitro recombination: an effective tool for directed evolution", Nucleic Acids Res, (19980000), vol. 26, doi:10.1093/nar/26.2.681, pages 681 - 683, XP002162556

DOI:   http://dx.doi.org/10.1093/nar/26.2.681
    - VOLKOV, A. A.Z. SHAOF. H. ARNOLD, "Recombination and chimeragenesis by in vitro heteroduplex formation and in vivo repair", Nucleic Acids Res, (19990000), vol. 27, page el8
    - VOLKOV, A. A.Z. SHAOF. H. ARNOLD, "Random chimeragenesis by heteroduplex recombination", Methods Enzymol., (20000000), vol. 328, doi:10.1016/S0076-6879(00)28412-7, pages 456 - 463, XP000990804

DOI:   http://dx.doi.org/10.1016/S0076-6879(00)28412-7
    - COCO, W. M.W. E. LEVINSONM. J. CRISTH. J. HEKTORA. DARZINSP. T. PIENKOSC. H. SQUIRESD. J. MONTICELLO, "DNA shuffling method for generating highly recombined genes and evolved enzymes", Nat.Biotechnol, (20010000), vol. 19, doi:10.1038/86744, pages 354 - 359, XP002185585

DOI:   http://dx.doi.org/10.1038/86744
    - LEE, S. H.E. J. RYUM. J. KANGE.-S. WANGZ. C. Y. PIAOK. J. J. JUNGY. SHIN, "A new approach to directed gene evolution by recombined extension on truncated templates (RETT", J.Molec.Catalysis, (20030000), vol. 26, pages 119 - 129
    - BERGQUIST, P. L.M. D. GIBBS, "Degenerate oligonucleotide gene shuffling", Methods MoLBiol, (20070000), vol. 352, pages 191 - 204
    - BERGQUIST, P. L.R. A. REEVESM. D. GIBBS, "Degenerate oligonucleotide gene shuffling (DOGS) and random drift mutagenesis (RNDM): two complementary techniques for enzyme evolution", Biomol.Eng, (20050000), vol. 22, doi:10.1016/j.bioeng.2004.10.002, pages 63 - 72, XP004862547

DOI:   http://dx.doi.org/10.1016/j.bioeng.2004.10.002
    - GIBBS, M. D.K. M NEVALAINENP. L. BERGQUIST, "Degenerate oligonucleotide gene shuffling (DOGS): a method for enhancing the frequency of recombination with family shuffling", Gene, (20010000), vol. 271, doi:10.1016/S0378-1119(01)00506-6, pages 13 - 20, XP004245489

DOI:   http://dx.doi.org/10.1016/S0378-1119(01)00506-6
    - OSTERMEIER et al., Proc NatlAcadSci US.A., (19990000), vol. 96, pages 3562 - 3567
    - OSTERMEIER et al., Nat.Biotechnol., (19990000), vol. 17, pages 1205 - 1209
    - LUTZ, S.M. OSTERMEIERS. J. BENKOVIC, "Rapid generation of incremental truncation libraries for protein engineering using alpha-phosphothioate nucleotides", Nucleic Acids Res, (20010000), vol. 29, doi:10.1093/nar/29.18.3873, page E16, XP002451644

DOI:   http://dx.doi.org/10.1093/nar/29.18.3873
    - LUTZ et al., Proc Natl Acad Sci U S.A., (20010000), vol. 98, pages 11248 - 11253
    - BERGQUIST et al., BiomolEng., (20050000), vol. 22, pages 63 - 72
    - WONG et al., Biotechnol J., (20080000), vol. 3, pages 74 - 82
    - MULLER et al., Nucleic Acids Res, (20050000), vol. 33, page el 17
    - WONG et al., Anal.Biochem., (20050000), vol. 341, pages 187 - 189
    - NESS et al., Nat.Biotechnol, (20020000), vol. 20, pages 1251 - 1255
    - SIEBER, V.C. A. MARTINEZF. H. ARNOLD, "Libraries of hybrid proteins from distantly related sequences", Nat.Biotechnol, (20010000), vol. 19, doi:10.1038/88129, pages 456 - 460, XP002236807

DOI:   http://dx.doi.org/10.1038/88129
    - KRETZ, K. A.T. H. RICHARDSONK. A. GRAYD. E. ROBERTSONX. TANJ. M. SHORT, "Gene site saturation mutagenesis: a comprehensive mutagenesis approach", Methods Enzymol., (20040000), vol. 388, pages 3 - 11
    - REIDHAAR-OLSON, J. F.J. U. BOWIER. M. BREYERJ. C. HUK. L. KNIGHTW. A. LIMM. C. MOSSINGD. A. PARSELLK. R. SHOEMAKERR. T. SAUER, "Random mutagenesis of protein sequences using oligonucleotide cassettes", Methods Enzymol., (19910000), vol. 208, doi:10.1016/0076-6879(91)08029-H, pages 564 - 586, XP008070927

DOI:   http://dx.doi.org/10.1016/0076-6879(91)08029-H
    - REETZ, M. T.S. WILENSEKD. ZHAK. E. JAEGER, "Directed Evolution of an Enantioselective Enzyme through Combinatorial Multiple-Cassette Mutagenesis", Angew.Chem.Int.Ed EngL, (20010000), vol. 40, doi:10.1002/1521-3773(20011001)40:19<3589::AID-ANIE3589>3.0.CO;2-X, pages 3589 - 3591, XP002214680

DOI:   http://dx.doi.org/10.1002/1521-3773(20011001)40:19<3589::AID-ANIE3589>3.0.CO;2-X
    - SELIFONOVA, O.F. VALLEV. SCHELLENBERGER, "Rapid evolution of novel traits in microorganisms", Appl Environ Microbiol, (20010000), vol. 67, doi:10.1128/AEM.67.8.3645-3649.2001, pages 3645 - 3649, XP001021467

DOI:   http://dx.doi.org/10.1128/AEM.67.8.3645-3649.2001
    - WINTERCOWORKERS, J. Mol. Biol., (19960000), vol. 260, pages 359 - 3680
    - RAJPAL, A.N. BEYAZL. HABERG. CAPPUCCILLIH. YEER. R. BHATTT. TAKEUCHIR. A. LERNERR. CREA, "A general method for greatly improving the affinity of antibodies by using combinatorial libraries", Proc Natl Acad Sci U S.A, (20050000), vol. 102, doi:10.1073/pnas.0503543102, pages 8466 - 8471, XP002392777

DOI:   http://dx.doi.org/10.1073/pnas.0503543102
    - HAYES, R. J.J. BENTZIENM. L. ARYM. Y. HWANGJ. M. JACINTOJ. VIELMETTERA. KUNDUB. I. DAHIYAT, "Combining computational and experimental screening for rapid optimization of protein properties", Proc Natl Acad Sci U S.A, (20020000), vol. 99, doi:10.1073/pnas.212627499, pages 15926 - 15931, XP002340572

DOI:   http://dx.doi.org/10.1073/pnas.212627499
    - REETZ, M. T.J. D. CARBALLEIRA, "Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes", Nat.Protoc., (20070000), vol. 2, doi:10.1038/nprot.2007.72, pages 891 - 903, XP001538725

DOI:   http://dx.doi.org/10.1038/nprot.2007.72
    - REETZ, M. T.J. D. CARBALLEIRAA. VOGEL, "Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability", Angew.Chem.Int.Ed EngL, (20060000), vol. 45, pages 7745 - 7751
    - BURGARD et al., Biotechnol. Bioeng., (20030000), vol. 84, pages 647 - 657
    - BURGARD et al., Biotechnol. Prog., (20010000), vol. 17, pages 791 - 797
    - BURGARD et al., Bbtechnol. Bioengineer., (20030000), vol. 84, pages 647 - 657
    - FONGPALSSON, Nat. Genet., (20040000), vol. 36, pages 1056 - 1058
    - ALPER et al., Science, (20060000), vol. 314, pages 1565 - 1568
    - BURGARD et al., Biotechnol. Bioengineer., (20030000), vol. 84, pages 647 - 657
    - "GenBank", Database accession no. AAP42563.1
    - LIN et al., Biotechnol Bioeng., (20050000), vol. 90, pages 775 - 779
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