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EP About this file: EP1251179

EP1251179 - Method for protein expression starting from stabilized linear short DNA in cell-free in vitro transcription/translation systems with exonuclease-containing lysates or in a cellular system containing exonucleases [Right-click to bookmark this link]
Status	The application is deemed to be withdrawn Status updated on 06.03.2009 Database last updated on 19.10.2024
Most recent event Tooltip	06.03.2009	Application deemed to be withdrawn	published on 08.04.2009 [2009/15]
Applicant(s)	For:DE Roche Diagnostics GmbH Sandhofer Strasse 116 68305 Mannheim / DE
	For:AT BE CH CY DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR F. Hoffmann-La Roche AG Grenzacherstrasse 124 4002 Basel / CH
	[N/P]
Former [2002/43]	For:DE Roche Diagnostics GmbH Sandhofer Strasse 116 68305 Mannheim / DE
	For:AT BE CH CY DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR F. HOFFMANN-LA ROCHE AG Grenzacherstrasse 124 4002 Basel / CH
Inventor(s)	01 / Watzele, Manfred, Dr. Ringstrasse 2 82362 Weilheim / DE
	02 / Hoffmann, Thomas Ahornstrasse 4 68535 Neu-Edingen / DE
	03 / Nemetz, Cordula, Dr. Isarspitz 11 82515 Wolfratshausen / DE
	04 / Heindl, Dieter, Dr. Waldschmidtstrasse 1 82327 Tutzing / DE
	05 / Metzler, Thomas, Dr. Maistrasse 57 80337 München / DE
	06 / Mutter, Wolfgang, Dr. Am Neuland 1 82347 Bernried / DE
	[2002/43]
Application number, filing date	02008551.0	16.04.2002
	[2002/43]
Priority number, date	DE2001119005	18.04.2001 Original published format: DE 10119005
	[2002/43]
Filing language	EN
Procedural language	EN
Publication	Type:	A2 Application without search report
	No.:	EP1251179
	Date:	23.10.2002
	Language:	EN
	[2002/43]
	Type:	A3 Search report
	No.:	EP1251179
	Date:	04.06.2003
	[2003/23]
Search report(s)	(Supplementary) European search report - dispatched on:	EP	24.04.2003
Classification	IPC:	C12N15/68	[2002/43]
	CPC:	C12N15/68 (EP,US); C12N15/10 (EP,US)
Designated contracting states	AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LI, LU, MC, NL, PT, SE, TR [2002/43]
Title	German:	Methode für Proteinexpression ausgehend von stabilisierter linearer kurzer DNA in einem zellfreien in vitro Transkriptions-/Translationssystem mit exonukleaseenthaltenden Lysaten oder in einem zellulären exonukleaseenthaltenden System	[2002/43]
	English:	Method for protein expression starting from stabilized linear short DNA in cell-free in vitro transcription/translation systems with exonuclease-containing lysates or in a cellular system containing exonucleases	[2002/43]
	French:	Méthode pour exprimer une protéine d'après de l'ADN stabilisé linéaire et court dans un système de transcription/translation in vitro sans cellules avec des lysats contenant de l'exonucléase ou dans un système cellulaire qui contient des exonucléases	[2002/43]
Examination procedure	04.12.2003	Examination requested [2004/06]
	29.01.2004	Despatch of a communication from the examining division (Time limit: M06)
	29.07.2004	Reply to a communication from the examining division
	27.01.2006	Despatch of a communication from the examining division (Time limit: M04)
	29.05.2006	Reply to a communication from the examining division
	04.06.2008	Despatch of a communication from the examining division (Time limit: M04)
	15.10.2008	Application deemed to be withdrawn, date of legal effect [2009/15]
	20.11.2008	Despatch of communication that the application is deemed to be withdrawn, reason: reply to the communication from the examining division not received in time [2009/15]
Fees paid	Renewal fee
	15.04.2004	Renewal fee patent year 03
	04.04.2005	Renewal fee patent year 04
	27.03.2006	Renewal fee patent year 05
	10.04.2007	Renewal fee patent year 06
	Penalty fee
	Additional fee for renewal fee
	30.04.2008	07 M06 Not yet paid
Opt-out from the exclusive Tooltip competence of the Unified Patent Court	See the Register of the Unified Patent Court for opt-out data
	Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Documents cited:	Search	[DA]US5571690 (HECHT SIDNEY M [US]) [DA] 1,17-21 * the whole document *;
		[DA]US6010907 (KMIEC ERIC B [US], et al) [DA] 1,18-21 * the whole document *;
		[DXY] - YANG H-L ET AL, "CELL-FREE COUPLED TRANSCRIPTION TRANSLATION SYSTEM FOR INVESTIGATION OF LINEAR DNA SEGMENTS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, 1980 (RECD. 1981), (1980), vol. 77, no. 12, ISSN 0027-8424, pages 7029 - 7033, XP001085422 [DX] 1,18-21 * abstract * * page 7029, column 1, paragraph 1 - paragraph 2 * * page 7030, column 1, paragraph 1 * * page 7030, column 2, paragraph L * [Y] 1-3 DOI: http://dx.doi.org/10.1073/pnas.77.12.7029
		[Y] - WILLIAMS J G K ET AL, "PARTIAL PURIFICATION AND PROPERTIES OF AN EXO NUCLEASE INHIBITOR INDUCED BY BACTERIO PHAGE MU-1", JOURNAL OF VIROLOGY, (1981), vol. 39, no. 2, ISSN 0022-538X, pages 548 - 558, XP001087798 [Y] 1-3 * abstract * * page 552, column 2, paragraph 2 - page 555, column 1, paragraph 1 *
		[Y] - BISWAS INDRANIL ET AL, "A 7-base-pair sequence protects DNA from exonucleolytic degradation in Lactococcus lactis.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, 1995, (1995), vol. 92, no. 6, ISSN 0027-8424, pages 2244 - 2248, XP001084036 [Y] 1-3 * the whole document * DOI: http://dx.doi.org/10.1073/pnas.92.6.2244
		[DY] - VERHEIJEN J C ET AL, "Incorporation of a 4-hydroxy-N-acetylprolinol nucleotide analogue improves the 3'-exonuclease stability of 2'-5'-oligoadenylate-antise nse conjugates", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, OXFORD, GB, (200004), vol. 10, no. 8, ISSN 0960-894X, pages 801 - 804, XP004203500 [DY] 1-3 * the whole document * DOI: http://dx.doi.org/10.1016/S0960-894X(00)00100-1
		[DY] - PANDOLFI DANIELA ET AL, "Evaluation of different types of end-capping modifications on the stability of oligonucleotides toward 3'- and 5'-exonucleases.", NUCLEOSIDES & NUCLEOTIDES, (1999), vol. 18, no. 9, ISSN 0732-8311, pages 2051 - 2069, XP001146882 [DY] 1-3 * abstract *
		[Y] - DURRENBERGER F ET AL, "COVALENTLY BOUND VIR-D2 PROTEIN OF AGROBACTERIUM-TUMEFACIENS PROTECTS THE T-DNA FROM EXONUCLEOLYTIC DEGRADATION", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, 1989, (1989), vol. 86, no. 23, ISSN 0027-8424, pages 9154 - 9158, XP001084199 [Y] 1-3 * abstract * * page 9154, column 1, paragraph L - column 2, paragraph 1 * DOI: http://dx.doi.org/10.1073/pnas.86.23.9154
		[XD] - YOSHIZAWA SATOKO ET AL, "Nuclease resistance of an extraordinarily thermostable mini-hairpin DNA fragment, d(GCGAAGC) and its application to in vitro protein synthesis.", NUCLEIC ACIDS RESEARCH, (1994), vol. 22, no. 12, ISSN 0305-1048, pages 2217 - 2221, XP001084025 [XD] 1,5-13 * page 2218, column 2, paragraph 4 - page 2219, column 1, paragraph 1 * * page 2220, column 1, paragraph 1 * * page 2220, column 2, paragraph L - page 2221, column 1, paragraph 1 *
		[XD] - HIRAO I ET AL, "STABILIZATION OF MRNA IN AN ESCHERICHIA COLI CELL-FREE TRANSLATION SYSTEM", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, (19930401), vol. 321, no. 2/3, ISSN 0014-5793, pages 169 - 172, XP002041751 [XD] 1,5-13 * abstract * * page 169, column 1, paragraphs 1-3; figure 1 * * page 172, column 2, paragraph L * DOI: http://dx.doi.org/10.1016/0014-5793(93)80101-Y

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