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Extract from the Register of European Patents

EP About this file: EP1730499

EP1730499 - METHOD FOR THE DETECTION OF INTRACELLULAR PARAMETERS WITH LUMINESCENT PROTEIN PROBES FOR THE SCREENING OF MOLECULES CAPABLE OF ALTERING SAID PARAMETERS [Right-click to bookmark this link]
StatusNo opposition filed within time limit
Status updated on  02.07.2010
Database last updated on 02.07.2024
Most recent event   Tooltip08.01.2016Lapse of the patent in a contracting statepublished on 10.02.2016  [2016/06]
Applicant(s)For all designated states
Aequotech S.r.l.
Via Saragat 1
44100 Ferrara / IT
[2008/25]
Former [2006/50]For all designated states
Rizzuto, Rosario
Via Crescini 6/ter
35126 Padova / IT
For all designated states
Pozzan, Tullio
Via Brenta Bassa 73
30031 Dolvo / IT
For all designated states
Pinton, Paolo
Via L. Da Vinci 12
35132 Padova / IT
For all designated states
Brini, Marisa
Via Crescini 6/ter
35126 Padova / IT
For all designated states
Giorgi, Carlotta
Via Roma 113
46037 Roncoferraro / IT
Inventor(s)01 / RIZZUTO, Rosario
Via San Giovanni di Verdara 43
I-35137 Padova / IT
02 / POZZAN, Tullio
Via Brenta Bassa 73
I-30031 Dolvo / IT
03 / PINTON, Paolo
Via L. Da Vinci 12
I-35132 Padova / IT
04 / GIORGI, Carlotta
Via Roma 113
I-46037 Roncoferraro / IT
 [2009/35]
Former [2007/07]01 / RIZZUTO, Rosario
Via San Giovanni di Verdara 43
I-35126 Padova / IT
02 / POZZAN, Tullio
Via Brenta Bassa 73
I-30031 Dolvo / IT
03 / PINTON, Paolo
Via L. Da Vinci 12
I-35132 Padova / IT
04 / GIORGI, Carlotta
Via Roma 113
I-46037 Roncoferraro / IT
Former [2006/50]01 / RIZZUTO, Rosario
Via Crescini 6/ter
I-35126 Padova / IT
02 / POZZAN, Tullio
Via Brenta Bassa 73
I-30031 Dolvo / IT
03 / PINTON, Paolo
Via L. Da Vinci 12
I-35132 Padova / IT
04 / GIORGI, Carlotta
Via Roma 113
I-46037 Roncoferraro / IT
Representative(s)Iannone, Carlo Luigi, et al
Barzanò & Zanardo Roma S.p.A.
Via Piemonte 26
00187 Roma / IT
[N/P]
Former [2006/50]Iannone, Carlo Luigi, et al
Barzanò & Zanardo Roma S.p.A. Via Piemonte 26
00187 Roma / IT
Application number, filing date05718286.723.03.2005
[2006/50]
WO2005IB00795
Priority number, dateIT2004MI0059826.03.2004         Original published format: IT MI20040598
[2006/50]
Filing languageIT
Procedural languageEN
PublicationType: A2 Application without search report
No.:WO2005093429
Date:06.10.2005
Language:EN
[2005/40]
Type: A2 Application without search report 
No.:EP1730499
Date:13.12.2006
Language:EN
The application published by WIPO in one of the EPO official languages on 06.10.2005 takes the place of the publication of the European patent application.
[2006/50]
Type: B1 Patent specification 
No.:EP1730499
Date:26.08.2009
Language:EN
[2009/35]
Search report(s)International search report - published on:EP13.04.2006
ClassificationIPC:G01N21/64, G01N33/52, C07K14/435
[2006/50]
CPC:
G01N33/84 (EP,US); G01N33/6872 (EP,US); G01N2500/10 (EP,US)
Designated contracting statesAT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   MC,   NL,   PL,   PT,   RO,   SE,   SI,   SK,   TR [2006/50]
Extension statesALNot yet paid
BANot yet paid
HRNot yet paid
LVNot yet paid
MKNot yet paid
YUNot yet paid
TitleGerman:VERFAHREN ZUR DETEKTION VON INTRAZELLULÄREN PARAMETERN MIT LUMINESZIERENDEN PROTEINSONDEN ZUR SELEKTION VON MOLEKÜLEN, DIE DIESE PARAMETER ÄNDERN KÖNNEN[2006/50]
English:METHOD FOR THE DETECTION OF INTRACELLULAR PARAMETERS WITH LUMINESCENT PROTEIN PROBES FOR THE SCREENING OF MOLECULES CAPABLE OF ALTERING SAID PARAMETERS[2006/50]
French:PROCEDE DE DETECTION DE PARAMETRES INTRACELLULAIRES AU MOYEN DE SONDES PROTEIQUES LUMINESCENTES POUR LE CRIBLAGE DE MOLECULES CAPABLES DE MODIFIER LESDITS PARAMETRES[2006/50]
Entry into regional phase06.10.2005Translation filed 
24.10.2006National basic fee paid 
24.10.2006Designation fee(s) paid 
24.10.2006Examination fee paid 
Examination procedure03.04.2006Request for preliminary examination filed
International Preliminary Examining Authority: EP
24.10.2006Examination requested  [2006/50]
13.12.2007Despatch of a communication from the examining division (Time limit: M04)
07.04.2008Reply to a communication from the examining division
18.05.2009Communication of intention to grant the patent
14.07.2009Fee for grant paid
14.07.2009Fee for publishing/printing paid
Opposition(s)27.05.2010No opposition filed within time limit [2010/31]
Fees paidRenewal fee
21.03.2007Renewal fee patent year 03
28.03.2008Renewal fee patent year 04
26.02.2009Renewal fee patent year 05
Opt-out from the exclusive  Tooltip
competence of the Unified
Patent Court
See the Register of the Unified Patent Court for opt-out data
Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Lapses during opposition  TooltipAT26.08.2009
CY26.08.2009
CZ26.08.2009
DK26.08.2009
EE26.08.2009
FI26.08.2009
LT26.08.2009
NL26.08.2009
PL26.08.2009
RO26.08.2009
SE26.08.2009
SI26.08.2009
SK26.08.2009
TR26.08.2009
BG26.11.2009
GR27.11.2009
ES07.12.2009
IS26.12.2009
PT28.12.2009
HU27.02.2010
IE23.03.2010
LU23.03.2010
MC31.03.2010
[2016/06]
Former [2012/47]AT26.08.2009
CY26.08.2009
CZ26.08.2009
DK26.08.2009
EE26.08.2009
FI26.08.2009
LT26.08.2009
NL26.08.2009
PL26.08.2009
RO26.08.2009
SE26.08.2009
SI26.08.2009
SK26.08.2009
TR26.08.2009
BG26.11.2009
GR27.11.2009
ES07.12.2009
IS26.12.2009
PT28.12.2009
HU27.02.2010
IE23.03.2010
LU23.03.2010
MC31.03.2010
Former [2012/43]AT26.08.2009
CY26.08.2009
CZ26.08.2009
DK26.08.2009
EE26.08.2009
FI26.08.2009
LT26.08.2009
NL26.08.2009
PL26.08.2009
RO26.08.2009
SE26.08.2009
SI26.08.2009
SK26.08.2009
BG26.11.2009
GR27.11.2009
ES07.12.2009
IS26.12.2009
PT28.12.2009
HU27.02.2010
IE23.03.2010
LU23.03.2010
MC31.03.2010
Former [2012/42]AT26.08.2009
CY26.08.2009
CZ26.08.2009
DK26.08.2009
EE26.08.2009
FI26.08.2009
LT26.08.2009
NL26.08.2009
PL26.08.2009
RO26.08.2009
SE26.08.2009
SI26.08.2009
SK26.08.2009
BG26.11.2009
GR27.11.2009
ES07.12.2009
IS26.12.2009
PT28.12.2009
HU27.02.2010
IE23.03.2010
MC31.03.2010
Former [2011/07]AT26.08.2009
CY26.08.2009
CZ26.08.2009
DK26.08.2009
EE26.08.2009
FI26.08.2009
LT26.08.2009
NL26.08.2009
PL26.08.2009
RO26.08.2009
SE26.08.2009
SI26.08.2009
SK26.08.2009
BG26.11.2009
GR27.11.2009
ES07.12.2009
IS26.12.2009
PT28.12.2009
IE23.03.2010
MC31.03.2010
Former [2010/46]AT26.08.2009
CY26.08.2009
CZ26.08.2009
DK26.08.2009
EE26.08.2009
FI26.08.2009
LT26.08.2009
NL26.08.2009
PL26.08.2009
RO26.08.2009
SE26.08.2009
SI26.08.2009
SK26.08.2009
BG26.11.2009
GR27.11.2009
ES07.12.2009
IS26.12.2009
PT28.12.2009
MC31.03.2010
Former [2010/23]AT26.08.2009
CY26.08.2009
CZ26.08.2009
DK26.08.2009
EE26.08.2009
FI26.08.2009
LT26.08.2009
NL26.08.2009
PL26.08.2009
RO26.08.2009
SE26.08.2009
SI26.08.2009
SK26.08.2009
BG26.11.2009
ES07.12.2009
IS26.12.2009
PT28.12.2009
Former [2010/20]AT26.08.2009
CY26.08.2009
DK26.08.2009
EE26.08.2009
FI26.08.2009
LT26.08.2009
NL26.08.2009
PL26.08.2009
SE26.08.2009
SI26.08.2009
BG26.11.2009
ES07.12.2009
IS26.12.2009
PT28.12.2009
Former [2010/19]AT26.08.2009
CY26.08.2009
FI26.08.2009
LT26.08.2009
NL26.08.2009
PL26.08.2009
SE26.08.2009
SI26.08.2009
BG26.11.2009
ES07.12.2009
IS26.12.2009
PT28.12.2009
Former [2010/17]AT26.08.2009
CY26.08.2009
FI26.08.2009
LT26.08.2009
NL26.08.2009
PL26.08.2009
SE26.08.2009
SI26.08.2009
BG26.11.2009
IS26.12.2009
PT28.12.2009
Former [2010/16]AT26.08.2009
CY26.08.2009
FI26.08.2009
LT26.08.2009
NL26.08.2009
PL26.08.2009
SE26.08.2009
SI26.08.2009
IS26.12.2009
PT28.12.2009
Former [2010/12]AT26.08.2009
FI26.08.2009
LT26.08.2009
NL26.08.2009
PL26.08.2009
SE26.08.2009
SI26.08.2009
IS26.12.2009
Cited inInternational search[A]US6566083  (THASTRUP OLE [DK], et al);
 [X]WO03082904  (CHEMICON INTERNAT INC [US]) [X] 1-5,31,32,43-46 * figure 1; claims 66-95 *;
 [X]  - TORFS HERBERT ET AL, "Recombinant aequorin as a reporter for receptor-mediated changes of intracellular Ca2+-levels in Drosophila S2 cells", INVERTEBRATE NEUROSCIENCE, (200204), vol. 4, no. 3, ISSN 1354-2516, pages 119 - 124, XP002348989 [X] 1-5,21,31,32,43-46 * the whole document *

DOI:   http://dx.doi.org/10.1007/s10158-001-0013-2
 [X]  - LE POUL EMMANUEL ET AL, "Adaptation of aequorin functional assay to high throughput screening", JOURNAL OF BIOMOLECULAR SCREENING, (200202), vol. 7, no. 1, ISSN 1087-0571, pages 57 - 65, XP009055395 [X] 1-5,21,31,32,43-46 * the whole document *
 [X]  - BRINI M ET AL, "Nuclear targeting of aequorin: A new approach for measuring nuclear Ca-2+ concentration in intact cells", CELL CALCIUM, (1994), vol. 16, no. 4, ISSN 0143-4160, pages 259 - 268, XP009055350 [X] 31-34,42 * the whole document *

DOI:   http://dx.doi.org/10.1016/0143-4160(94)90089-2
 [X]  - CHIESA A ET AL, "RECOMBINANT AEQUORIN AND GREEN FLUORESCENT PROTEIN AS VALUABLE TOOLS IN THE STUDY OF CELL SIGNALLING", BIOCHEMICAL JOURNAL, THE BIOCHEMICAL SOCIETY, LONDON, GB, (20010401), vol. 355, no. 1, ISSN 0264-6021, pages 1 - 12, XP009013205 [X] 31,32,43 * figure 2 *

DOI:   http://dx.doi.org/10.1042/0264-6021:3550001
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