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Extract from the Register of European Patents

EP About this file: EP1688500

EP1688500 - Overexpression of phytase genes in yeast systems [Right-click to bookmark this link]
StatusPatent revoked
Status updated on  16.05.2022
Database last updated on 20.03.2026
Most recent event   Tooltip20.05.2022Revocation of patentpublished on 22.06.2022  [2022/25]
Applicant(s)For all designated states
CORNELL RESEARCH FOUNDATION, INC.
Cornell Center for Technology Enterprise & Commercialization 395 Pine Tree Road, Suite 310
Ithaca, NY 14850 / US
[2007/51]
Former [2006/32]For all designated states
CORNELL RESEARCH FOUNDATION, INC.
20 Thornwood Drive, Suite 105
Ithaca, NY 14580 / US
Inventor(s)01 / Lei, Xingen
114 Lexington Drive W.
Ithaca, NY 14850 / US
 [2006/32]
Representative(s)Grünecker Patent- und Rechtsanwälte PartG mbB
Leopoldstraße 4
80802 München / DE
[2016/01]
Former [2011/23]Grünecker, Kinkeldey, Stockmair & Schwanhäusser Anwaltssozietät
Leopoldstrasse 4
80802 München / DE
Former [2009/19]Vogelsang-Wenke, Heike
Grünecker Kinkeldey Stockmair & Schwanhäusser Anwaltssozietät Leopoldstrasse 4
80802 München / DE
Former [2006/32]Jones, Stephen Anthony, et al
AdamsonJones BioCity Nottingham Pennyfoot Street
Nottingham NG1 1GF / GB
Application number, filing date06075318.323.06.1999
[2006/32]
Priority number, dateUS1998010476925.06.1998         Original published format: US 104769
[2006/32]
Filing languageEN
Procedural languageEN
PublicationType: A2 Application without search report 
No.:EP1688500
Date:09.08.2006
Language:EN
[2006/32]
Type: A3 Search report 
No.:EP1688500
Date:05.11.2008
[2008/45]
Type: B1 Patent specification 
No.:EP1688500
Date:30.12.2015
Language:EN
[2015/53]
Search report(s)(Supplementary) European search report - dispatched on:EP07.10.2008
ClassificationIPC:C12N15/55, C12N9/16, A23K1/165, A23L1/015
[2006/32]
CPC:
C12N9/16 (EP,US); C12N15/52 (KR); A23K20/189 (EP,US);
A23K50/30 (EP,US); A23K50/60 (EP,US); C12P7/18 (EP,US)
Designated contracting statesAT,   BE,   CH,   CY,   DE,   DK,   ES,   FI,   FR,   GB,   GR,   IE,   IT,   LI,   LU,   MC,   NL,   PT,   SE [2016/01]
Former [2006/32]AT,  BE,  CH,  CY,  DE,  DK,  ES,  FI,  FR,  GB,  GR,  IE,  IT,  LI,  LU,  MC,  NL,  PT,  SE 
TitleGerman:Überexpession von Phytase In Hefen[2006/32]
English:Overexpression of phytase genes in yeast systems[2006/32]
French:Surexpression de la Phytase dans la levure[2006/32]
Examination procedure09.02.2006Examination requested  [2006/32]
16.06.2009Despatch of a communication from the examining division (Time limit: M07)
04.03.2010Despatch of communication that the application is deemed to be withdrawn, reason: reply to the communication from the examining division not received in time
14.05.2010Reply to a communication from the examining division
11.06.2012Despatch of a communication from the examining division (Time limit: M06)
21.12.2012Reply to a communication from the examining division
03.06.2014Despatch of a communication from the examining division (Time limit: M04)
13.10.2014Reply to a communication from the examining division
17.11.2014Despatch of a communication from the examining division (Time limit: M06)
22.05.2015Reply to a communication from the examining division
16.07.2015Communication of intention to grant the patent
29.10.2015Fee for grant paid
29.10.2015Fee for publishing/printing paid
29.10.2015Receipt of the translation of the claim(s)
Parent application(s)   TooltipEP99935340.2  / EP1090129
Divisional application(s)EP15200497.4  / EP3020815
The date of the Examining Division's first communication in respect of the earliest application for which a communication has been issued (EP19990935340) is  11.08.2003
Opposition(s)Opponent(s)01  30.09.2016  06.10.2016  ADMISSIBLE
Kaesler Nutrition GmbH
Zeppelinstrasse 3
27472 Cuxhaven / DE
Opponent's representative
V.O.
P.O. Box 87930
Carnegieplein 5
2508 DH Den Haag / NL
 [2017/29]
Former [2016/45]
Opponent(s)01  30.09.2016  06.10.2016  ADMISSIBLE
Lohmann Animal Nutrition GmbH
Zeppelinstrasse 3
27472 Cuxhaven / DE
Opponent's representative
V.O.
P.O. Box 87930
Carnegieplein 5
2508 DH Den Haag / NL
08.11.2016Invitation to proprietor to file observations on the notice of opposition
17.03.2017Reply of patent proprietor to notice(s) of opposition
06.03.2018Date of oral proceedings
17.04.2018Despatch of interlocutory decision in opposition
17.04.2018Despatch of minutes of oral proceedings
25.02.2022Legal effect of revocation of patent [2022/25]
02.03.2022Despatch of communication that the patent will be revoked
Appeal following opposition26.06.2018Appeal received No.  T1659/18
27.08.2018Statement of grounds filed
25.02.2022Result of appeal procedure: revocation of the patent
02.03.2022Despatch of the decision of the Board of Appeal
27.06.2018Appeal received No.  T1659/18
22.08.2018Statement of grounds filed
25.02.2022Result of appeal procedure: revocation of the patent
02.03.2022Despatch of the decision of the Board of Appeal
Request for further processing for:The application is deemed to be withdrawn due to failure to reply to the examination report
14.05.2010Request for further processing filed
14.05.2010Full payment received (date of receipt of payment)
Fees paidRenewal fee
09.02.2006Renewal fee patent year 03
09.02.2006Renewal fee patent year 04
09.02.2006Renewal fee patent year 05
09.02.2006Renewal fee patent year 06
09.02.2006Renewal fee patent year 07
22.05.2006Renewal fee patent year 08
27.06.2007Renewal fee patent year 09
25.06.2008Renewal fee patent year 10
26.06.2009Renewal fee patent year 11
25.06.2010Renewal fee patent year 12
27.06.2011Renewal fee patent year 13
26.06.2012Renewal fee patent year 14
27.06.2013Renewal fee patent year 15
30.06.2014Renewal fee patent year 16
29.06.2015Renewal fee patent year 17
Opt-out from the exclusive  Tooltip
competence of the Unified
Patent Court		 See the Register of the Unified Patent Court for opt-out data
Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Lapses during opposition  TooltipCY30.12.2015
MC30.12.2015
LU23.06.2016
GB22.06.2019
IE23.06.2019
ES24.06.2019
PT02.07.2019
[2020/47]
Former [2019/48]CY30.12.2015
MC30.12.2015
LU23.06.2016
GB22.06.2019
IE23.06.2019
PT02.07.2019
Former [2019/47]CY30.12.2015
MC30.12.2015
LU23.06.2016
GB22.06.2019
Former [2018/29]CY30.12.2015
MC30.12.2015
LU23.06.2016
Former [2017/07]MC30.12.2015
Documents cited:Search[XY] EP0420358  (GIST BROCADES NV et al.)
 [XY] EP0684313  (HOFFMANN LA ROCHE et al.)
 [X] WO9748812  (CANADA AGRICULTURE et al.)
 [X] GB2316082  (FINNFEEDS INT LTD et al.)
 [PX] WO9908539  (DIVERSA CORP et al.)
 [X] WO9820139  (FINNFEEDS INT LTD et al.)
 [A] WO9805785  (BIOCEM et al.)
 [X] EP0699762  (MITSUI TOATSU CHEMICALS et al.) [X] 1,6-12,15,16,22,27-32,39,40 * abstract * * page 4, lines 3,4 * * page 5, lines 9-12 * * page 10, line 29 - page 11, line 26 * * page 14, line 28 - page 16, line 9 * * page 23, lines 4-19; figures 4-6 * * page 30, line 10 - page 36, line 11 * * page 42 - page 43; tables 4,5 *
 [X] WO9735017  (NOVO NORDISK BIOTECH INC et al.) [X] 1,6-12,15,16,22,27-32,39,40 * abstract * * page 9, paragraph 4 *
 [Y]   YAO BIN ET AL.: "Recombinant Pichia pastoris overexpressing bioactive Phytase", SCIENCE IN CHINA SERIES C. LIFE SCIENCES, vol. 41, no. 3, June 1998 (1998-06-01), pages 330 - 336, XP002118576
 [X]   WEILIN SUN ET AL.: "Expression of Aspergillus niger Phytase in yeast Saccharomyces cerevisiae for poultry diet supplementation", POULTRY SCIENCE, vol. 76, no. suppl. 1, 1997, CHAMPAIGN, IL, US, pages 5, XP002118708, ISSN: 0032-5791
 [A]   WODZINSKI R.J. AND ULLAH A.H.J.: "Phytase", ADVANCES IN APPLIED MICROBIOLOGY, vol. 42, 1996, NEW YORK, NY, US, pages 263 - 302, XP002118577
 [Y]   MINAMIGUCHI K.: "Secretive expression of the Aspergillus aculeatus Cellulase (FI-CMCase) by Saccharomyces cerevisiae", JOURNAL OF FERMENTATION AND BIOENGINEERING, vol. 79, no. 4, 1995, SOCIETY OF FERMENTATION TECHNOLOGY, JP, pages 363 - 366, XP002119099, ISSN: 0922-338X

DOI:   http://dx.doi.org/10.1016/0922-338X(95)93996-W
 [Y]   KANAI T. ET AL.: "Recombinant thermostable Cycloinulo-oligosaccharide Fructanotransferase produced by Saccharomyces cerevisiae", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 63, no. 12, December 1997 (1997-12-01), WASHINGTON, DC, US, pages 4956 - 4960, XP002129678, ISSN: 0099-2240
 [Y]   FIEROBE H.-P. ET AL.: "Overexpression and characterization of Aspergillus awamori wild-type and mutant Glucoamylase secreted by the methylotrophic yeast Pichia pastoris: comparison with wild-type recombinant Glucoamylase produced using Saccharomyces cerevisiae and Aspergillus niger as hosts", PROTEIN EXPRESSION AND PURIFICATION, vol. 9, no. 2, March 1997 (1997-03-01), ACADEMIC PRESS, US, pages 159 - 170, XP002129679, ISSN: 1046-5928

DOI:   http://dx.doi.org/10.1006/prep.1996.0689
 [XY]   MELDGAARD M. AND SVENDSEN I.: "Different effects of N-glycosylation on the thermostability of highly homologous bacterial (1,3-1,4)-beta-Glucanases secreted from yeasts", MICROBIOLOGY, vol. 140, no. part 1, January 1994 (1994-01-01), pages 159 - 166, XP002129680 [Y] 4,5,19-21,25,26
 [Y]   CURRY C. ET AL.: "Expression and secretion of a Cellulomonas fimi Exoglucanase in Saccharomyces cerevisiae", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 54, no. 2, February 1988 (1988-02-01), WASHINGTON, DC, US, pages 476 - 484, XP002129681, ISSN: 0099-2240 [Y] 4,5,25,26 * the whole document *
 [XY]   OLSEN O. ET AL.: "Improvement of bacterial beta-Glucanase thermostability by glycosylation", JOURNAL OF GENERAL MICROBIOLOGY, vol. 137, no. 3, 1 March 1991 (1991-03-01), pages 579 - 585, XP000566763, ISSN: 0001-2961 [Y] 19-21
 [X]   SOLOVICOV A. ET AL.: "High-yield production of Saccharomycopsis fibuligera Glucoamylase in Escherichia coli, refolding and comparison of the nonglycosylated and glycosylated enzyme forms", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 224, 1996, ACADEMIC PRESS INC. ORLANDO, FL, US, pages 790 - 795, XP002129682, ISSN: 0006-291X [X] 1-3,6-11,15-17,22,23,27-31,33,39,40 * the whole document *

DOI:   http://dx.doi.org/10.1006/bbrc.1996.1101
Examination  DASSA J ET AL: "THE COMPLETE NUCLEOTIDE SEQUENCE OF THE ESCHERICHIA COLI GENE APPA REVEALS SIGNIFICANT HOMOLOGY BETWEEN PH 2.5 ACID PHOSPHATASE AND GLUCOSE-1-PHOSPHATASE", JOURNAL OF BACTERIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, DC; US, vol. 172, no. 9, 1 September 1990 (1990-09-01), pages 5497 - 5500, XP002911650, ISSN: 0021-9193
   OSTANIN K ET AL: "Overexpression, site-directed mutagenesis, and mechanism of Escherichia coli acid phosphatase", JOURNAL OF BIOLOGICAL CHEMISTRY, THE AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, INC, US, vol. 267, no. 32, 15 November 1992 (1992-11-15), pages 22830 - 22836, XP002129674, ISSN: 0021-9258
   GREINER R ET AL: "Purification and Characterization of Two Phytases from Escherichia coli", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, ACADEMIC PRESS, US, vol. 303, no. 1, 15 May 1993 (1993-05-15), pages 107 - 113, XP024753280, ISSN: 0003-9861, [retrieved on 19930515], DOI: 10.1006/ABBI.1993.1261

DOI:   http://dx.doi.org/10.1006/abbi.1993.1261
   TOUATI E ET AL: "THE STRUCTURE OF THE PROMOTER AND AMINO TERMINAL REGION OF THE PH 2.5 ACID PHOSPHATASE STRUCTURAL GENE (APPA) OF E. COLI: A NEGATIVE CONTROL OF TRANSCRIPTION MEDIATED BY CYCLIC AMP", BIOCHIMIE, MASSON, PARIS, FR, vol. 69, 1 January 1987 (1987-01-01), pages 215 - 221, XP000949918, ISSN: 0300-9084, DOI: 10.1016/0300-9084(87)90045-9

DOI:   http://dx.doi.org/10.1016/0300-9084(87)90045-9
OppositionEP1230350
   WODZINSKI R J AND ULLAH A H J: "Phytase", ADVANCES IN APPLIED MICROBIOLOGY,, vol. 42, 1996, pages 263 - 302, XP002118577
   KONIETZNY U; GREINER R: "MODEL SYSTEMS FOR DEVELOPING DETECTION METHODS FOR FOODS DERIVING FROM GENETIC ENGINEERING", JOURNAL OF FOOD COMPOSITION AND ANALYSIS,, vol. 10, no. 1, 1997, XP000878550

DOI:   http://dx.doi.org/10.1006/jfca.1997.0521
   JANNE KEROVUO, MARKO LAURAEUS, PIVI NURMINEN, NISSE KALKKINEN, JUHA APAJALAHTI: "Isolation, Characterization, Molecular Gene Cloning, and Sequencing of a Novel Phytase from Bacillus subtilis", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 64, no. 6, 1 June 1998 (1998-06-01), pages 2079 - 2085, XP055454048
   R.GREINER, K.D. JANY: "CHARACTERIZATION OF A PHYTASE FROM ESCHERICHIA COLI /Abstract/", BIOLOGICAL CHEMISTRY HOPPE-SEYLER,, vol. 372, 1991, pages 664 - 665, XP000949912
   VEGARUD G, CHRISTNSEN TB.: "Glycosylation of proteins: A new method of enzyme stabilization", vol. 17, no. 9, September 1975 (1975-09-01), pages 1391 - 1397, XP055454056
   CURRY C; ET AL: "Expresion and secretion of a Cellulomonas fimi Exoglucanase in Saccharomyces cerevisiae", APPLIED AND ENVIRONMENTAL MICROBIOLOGY,, vol. 54, no. 2, 1 February 1988 (1988-02-01), pages 476 - 484, XP002129681
   OLSEN O; THOMSEN K K: "IMRPOVEMENT OF BACTERIAL BETA-FLUCANASE THERMOSTABILITY BY GLYCOSYLATION", JOURNAL OF GENERAL MICROBIOLOGY,, vol. 137, no. 3, 1 March 1991 (1991-03-01), pages 579 - 585, XP000566763
   MELDGAARD M AND SVENDSEN I: "Different effects of N-glycosylation on the thermostability of highly homologous bacterial (1,3-1,4)-beta-Glucanases secreted from yeasts", MICROBIOLOGY,, vol. 140, no. 1, 1994, pages 159 - 166, XP002129680
   KANAI ET AL.: "Recombinant thermostable Cycloinulo-oligosaccharide Fructanotransferase produced by saccharomyces cerevisiae", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 63, no. 12, December 1997 (1997-12-01), pages 4956 - 4960, XP002129678
   WEILIN SUN: "Cloning and expression of calpain and phytase genes for the improvement of animal growth and nutrition", THESIS PURDUE UNIVERSITY, December 1996 (1996-12-01), pages 59 - 80, 160-184, XP055454057
   STAHL ET AL.: "Comparison of extracellular Escherichia coli AppA phytases expressed in Streptomyces lividans and Pichia pastoris", BIOTECHNOLOGY LETTERS, vol. 25, 2003, pages 827 - 831, XP055454052
   KIM ET AL.: "Enhancing thermostability of Escherichia coli phytase AppA2 by error-prone PCR", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 79, 2008, pages 69 - 75, XP019623565
The EPO accepts no responsibility for the accuracy of data originating from other authorities; in particular, it does not guarantee that it is complete, up to date or fit for specific purposes.