| EP2401364 - DIFFERENTIATION OF PLURIPOTENT CELLS [Right-click to bookmark this link] | Status | No opposition filed within time limit Status updated on 26.02.2016 Database last updated on 11.04.2026 | Most recent event Tooltip | 17.08.2018 | Lapse of the patent in a contracting state New state(s): BG | published on 19.09.2018 [2018/38] | Applicant(s) | For all designated states Cellular Dynamics International, Inc. University Research Park 525 Science Drive, Suite 200 Madison, WI 53711 / US | [2012/01] | Inventor(s) | 01 /
RAJESH, Deepika c/o Cellular Dynamics International Inc. University Research Park 525 Science Drive Suite 200 Madison, WI 53711 / US | 02 /
LEWIS, Rachel c/o CellularDynamics International Inc. University Research Park 525 Science Drive Suite 200 Madison, WI 53711 / US | [2012/01] | Representative(s) | Vossius & Partner Patentanwälte Rechtsanwälte mbB Siebertstrasse 3 81675 München / DE | [2015/17] |
| Former [2012/01] | Vossius & Partner Siebertstrasse 4 81675 München / DE | Application number, filing date | 10707179.7 | 01.03.2010 | WO2010US25776 | Priority number, date | US20090156304P | 27.02.2009 Original published format: US 156304 P | [2012/01] | Filing language | EN | Procedural language | EN | Publication | Type: | A1 Application with search report | No.: | WO2010099539 | Date: | 02.09.2010 | Language: | EN | [2010/35] | Type: | A1 Application with search report | No.: | EP2401364 | Date: | 04.01.2012 | Language: | EN | The application published by WIPO in one of the EPO official languages on 02.09.2010 takes the place of the publication of the European patent application. | [2012/01] | Type: | B1 Patent specification | No.: | EP2401364 | Date: | 22.04.2015 | Language: | EN | [2015/17] | Search report(s) | International search report - published on: | EP | 02.09.2010 | Classification | IPC: | C12N5/0781, C12N5/071 | [2012/01] | CPC: |
C12N5/0647 (EP,US);
A61P25/00 (EP);
A61P37/02 (EP);
A61P7/00 (EP);
A61P9/00 (EP);
C12N5/0692 (EP,US);
C12N2500/02 (EP,US);
C12N2501/115 (EP,US);
C12N2501/125 (EP,US);
C12N2501/14 (EP,US);
C12N2501/145 (EP,US);
C12N2501/155 (EP,US);
C12N2501/165 (EP,US);
C12N2501/22 (EP,US);
C12N2501/23 (EP,US);
| Designated contracting states | AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LI, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR [2012/01] | Extension states | AL | Not yet paid | BA | Not yet paid | ME | Not yet paid | RS | Not yet paid | Title | German: | DIFFERENZIERUNG PLURIPOTENTER ZELLEN | [2012/01] | English: | DIFFERENTIATION OF PLURIPOTENT CELLS | [2012/01] | French: | DIFFÉRENCIATION DE CELLULES PLURIPOTENTES | [2012/01] | Entry into regional phase | 27.09.2011 | National basic fee paid | 27.09.2011 | Designation fee(s) paid | 27.09.2011 | Examination fee paid | Examination procedure | 27.09.2011 | Examination requested [2012/01] | 27.03.2012 | Amendment by applicant (claims and/or description) | 24.01.2013 | Despatch of a communication from the examining division (Time limit: M06) | 02.08.2013 | Reply to a communication from the examining division | 10.10.2014 | Communication of intention to grant the patent | 18.02.2015 | Fee for grant paid | 18.02.2015 | Fee for publishing/printing paid | 18.02.2015 | Receipt of the translation of the claim(s) | Divisional application(s) | The date of the Examining Division's first communication in respect of the earliest application for which a communication has been issued is 24.01.2013 | Opposition(s) | 25.01.2016 | No opposition filed within time limit [2016/13] | Fees paid | Renewal fee | 14.03.2012 | Renewal fee patent year 03 | 11.03.2013 | Renewal fee patent year 04 | 12.03.2014 | Renewal fee patent year 05 | 10.03.2015 | Renewal fee patent year 06 |
| Opt-out from the exclusive Tooltip competence of the Unified Patent Court | See the Register of the Unified Patent Court for opt-out data | ||
| Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court. | Lapses during opposition Tooltip | HU | 01.03.2010 | AT | 22.04.2015 | BE | 22.04.2015 | BG | 22.04.2015 | CY | 22.04.2015 | CZ | 22.04.2015 | DK | 22.04.2015 | EE | 22.04.2015 | ES | 22.04.2015 | FI | 22.04.2015 | HR | 22.04.2015 | IT | 22.04.2015 | LT | 22.04.2015 | LV | 22.04.2015 | MC | 22.04.2015 | MK | 22.04.2015 | PL | 22.04.2015 | RO | 22.04.2015 | SE | 22.04.2015 | SI | 22.04.2015 | SK | 22.04.2015 | SM | 22.04.2015 | TR | 22.04.2015 | NO | 22.07.2015 | GR | 23.07.2015 | IS | 22.08.2015 | PT | 24.08.2015 | [2018/38] |
| Former [2018/31] | HU | 01.03.2010 | |
| AT | 22.04.2015 | ||
| BE | 22.04.2015 | ||
| CY | 22.04.2015 | ||
| CZ | 22.04.2015 | ||
| DK | 22.04.2015 | ||
| EE | 22.04.2015 | ||
| ES | 22.04.2015 | ||
| FI | 22.04.2015 | ||
| HR | 22.04.2015 | ||
| IT | 22.04.2015 | ||
| LT | 22.04.2015 | ||
| LV | 22.04.2015 | ||
| MC | 22.04.2015 | ||
| MK | 22.04.2015 | ||
| PL | 22.04.2015 | ||
| RO | 22.04.2015 | ||
| SE | 22.04.2015 | ||
| SI | 22.04.2015 | ||
| SK | 22.04.2015 | ||
| SM | 22.04.2015 | ||
| TR | 22.04.2015 | ||
| NO | 22.07.2015 | ||
| GR | 23.07.2015 | ||
| IS | 22.08.2015 | ||
| PT | 24.08.2015 | ||
| Former [2018/29] | HU | 01.03.2010 | |
| AT | 22.04.2015 | ||
| BE | 22.04.2015 | ||
| CY | 22.04.2015 | ||
| CZ | 22.04.2015 | ||
| DK | 22.04.2015 | ||
| EE | 22.04.2015 | ||
| ES | 22.04.2015 | ||
| FI | 22.04.2015 | ||
| HR | 22.04.2015 | ||
| IT | 22.04.2015 | ||
| LT | 22.04.2015 | ||
| LV | 22.04.2015 | ||
| MC | 22.04.2015 | ||
| MK | 22.04.2015 | ||
| MT | 22.04.2015 | ||
| PL | 22.04.2015 | ||
| RO | 22.04.2015 | ||
| SE | 22.04.2015 | ||
| SI | 22.04.2015 | ||
| SK | 22.04.2015 | ||
| SM | 22.04.2015 | ||
| TR | 22.04.2015 | ||
| NO | 22.07.2015 | ||
| GR | 23.07.2015 | ||
| IS | 22.08.2015 | ||
| PT | 24.08.2015 | ||
| Former [2018/28] | HU | 01.03.2010 | |
| AT | 22.04.2015 | ||
| BE | 22.04.2015 | ||
| CY | 22.04.2015 | ||
| CZ | 22.04.2015 | ||
| DK | 22.04.2015 | ||
| EE | 22.04.2015 | ||
| ES | 22.04.2015 | ||
| FI | 22.04.2015 | ||
| HR | 22.04.2015 | ||
| IT | 22.04.2015 | ||
| LT | 22.04.2015 | ||
| LV | 22.04.2015 | ||
| MC | 22.04.2015 | ||
| MK | 22.04.2015 | ||
| MT | 22.04.2015 | ||
| PL | 22.04.2015 | ||
| RO | 22.04.2015 | ||
| SE | 22.04.2015 | ||
| SI | 22.04.2015 | ||
| SK | 22.04.2015 | ||
| SM | 22.04.2015 | ||
| NO | 22.07.2015 | ||
| GR | 23.07.2015 | ||
| IS | 22.08.2015 | ||
| PT | 24.08.2015 | ||
| Former [2017/40] | AT | 22.04.2015 | |
| BE | 22.04.2015 | ||
| CZ | 22.04.2015 | ||
| DK | 22.04.2015 | ||
| EE | 22.04.2015 | ||
| ES | 22.04.2015 | ||
| FI | 22.04.2015 | ||
| HR | 22.04.2015 | ||
| IT | 22.04.2015 | ||
| LT | 22.04.2015 | ||
| LV | 22.04.2015 | ||
| MC | 22.04.2015 | ||
| MT | 22.04.2015 | ||
| PL | 22.04.2015 | ||
| RO | 22.04.2015 | ||
| SE | 22.04.2015 | ||
| SI | 22.04.2015 | ||
| SK | 22.04.2015 | ||
| NO | 22.07.2015 | ||
| GR | 23.07.2015 | ||
| IS | 22.08.2015 | ||
| PT | 24.08.2015 | ||
| Former [2016/49] | AT | 22.04.2015 | |
| BE | 22.04.2015 | ||
| CZ | 22.04.2015 | ||
| DK | 22.04.2015 | ||
| EE | 22.04.2015 | ||
| ES | 22.04.2015 | ||
| FI | 22.04.2015 | ||
| HR | 22.04.2015 | ||
| IT | 22.04.2015 | ||
| LT | 22.04.2015 | ||
| LV | 22.04.2015 | ||
| MC | 22.04.2015 | ||
| PL | 22.04.2015 | ||
| RO | 22.04.2015 | ||
| SI | 22.04.2015 | ||
| SK | 22.04.2015 | ||
| NO | 22.07.2015 | ||
| GR | 23.07.2015 | ||
| IS | 22.08.2015 | ||
| PT | 24.08.2015 | ||
| Former [2016/37] | AT | 22.04.2015 | |
| BE | 22.04.2015 | ||
| CZ | 22.04.2015 | ||
| DK | 22.04.2015 | ||
| EE | 22.04.2015 | ||
| ES | 22.04.2015 | ||
| FI | 22.04.2015 | ||
| HR | 22.04.2015 | ||
| IT | 22.04.2015 | ||
| LT | 22.04.2015 | ||
| LV | 22.04.2015 | ||
| PL | 22.04.2015 | ||
| RO | 22.04.2015 | ||
| SI | 22.04.2015 | ||
| SK | 22.04.2015 | ||
| NO | 22.07.2015 | ||
| GR | 23.07.2015 | ||
| IS | 22.08.2015 | ||
| PT | 24.08.2015 | ||
| Former [2016/24] | AT | 22.04.2015 | |
| CZ | 22.04.2015 | ||
| DK | 22.04.2015 | ||
| EE | 22.04.2015 | ||
| ES | 22.04.2015 | ||
| FI | 22.04.2015 | ||
| HR | 22.04.2015 | ||
| IT | 22.04.2015 | ||
| LT | 22.04.2015 | ||
| LV | 22.04.2015 | ||
| PL | 22.04.2015 | ||
| RO | 22.04.2015 | ||
| SI | 22.04.2015 | ||
| SK | 22.04.2015 | ||
| NO | 22.07.2015 | ||
| GR | 23.07.2015 | ||
| IS | 22.08.2015 | ||
| PT | 24.08.2015 | ||
| Former [2016/21] | AT | 22.04.2015 | |
| CZ | 22.04.2015 | ||
| DK | 22.04.2015 | ||
| EE | 22.04.2015 | ||
| ES | 22.04.2015 | ||
| FI | 22.04.2015 | ||
| HR | 22.04.2015 | ||
| IT | 22.04.2015 | ||
| LT | 22.04.2015 | ||
| LV | 22.04.2015 | ||
| PL | 22.04.2015 | ||
| RO | 22.04.2015 | ||
| SK | 22.04.2015 | ||
| NO | 22.07.2015 | ||
| GR | 23.07.2015 | ||
| IS | 22.08.2015 | ||
| PT | 24.08.2015 | ||
| Former [2016/10] | AT | 22.04.2015 | |
| CZ | 22.04.2015 | ||
| DK | 22.04.2015 | ||
| EE | 22.04.2015 | ||
| ES | 22.04.2015 | ||
| FI | 22.04.2015 | ||
| HR | 22.04.2015 | ||
| LT | 22.04.2015 | ||
| LV | 22.04.2015 | ||
| PL | 22.04.2015 | ||
| RO | 22.04.2015 | ||
| SK | 22.04.2015 | ||
| NO | 22.07.2015 | ||
| GR | 23.07.2015 | ||
| IS | 22.08.2015 | ||
| PT | 24.08.2015 | ||
| Former [2016/09] | AT | 22.04.2015 | |
| DK | 22.04.2015 | ||
| EE | 22.04.2015 | ||
| ES | 22.04.2015 | ||
| FI | 22.04.2015 | ||
| HR | 22.04.2015 | ||
| LT | 22.04.2015 | ||
| LV | 22.04.2015 | ||
| NO | 22.07.2015 | ||
| GR | 23.07.2015 | ||
| IS | 22.08.2015 | ||
| PT | 24.08.2015 | ||
| Former [2016/07] | AT | 22.04.2015 | |
| DK | 22.04.2015 | ||
| ES | 22.04.2015 | ||
| FI | 22.04.2015 | ||
| HR | 22.04.2015 | ||
| LT | 22.04.2015 | ||
| LV | 22.04.2015 | ||
| NO | 22.07.2015 | ||
| GR | 23.07.2015 | ||
| IS | 22.08.2015 | ||
| PT | 24.08.2015 | ||
| Former [2015/51] | AT | 22.04.2015 | |
| ES | 22.04.2015 | ||
| FI | 22.04.2015 | ||
| HR | 22.04.2015 | ||
| LT | 22.04.2015 | ||
| LV | 22.04.2015 | ||
| NO | 22.07.2015 | ||
| GR | 23.07.2015 | ||
| IS | 22.08.2015 | ||
| PT | 24.08.2015 | ||
| Former [2015/50] | ES | 22.04.2015 | |
| FI | 22.04.2015 | ||
| HR | 22.04.2015 | ||
| LT | 22.04.2015 | ||
| LV | 22.04.2015 | ||
| NO | 22.07.2015 | ||
| GR | 23.07.2015 | ||
| IS | 22.08.2015 | ||
| PT | 24.08.2015 | ||
| Former [2015/49] | ES | 22.04.2015 | |
| FI | 22.04.2015 | ||
| HR | 22.04.2015 | ||
| LT | 22.04.2015 | ||
| LV | 22.04.2015 | ||
| NO | 22.07.2015 | ||
| GR | 23.07.2015 | ||
| PT | 24.08.2015 | Cited in | International search | [Y] PARK CHANGWON ET AL: "A hierarchical order of factors in the generation of FLK1- and SCL-expressing hematopoietic and endothelial progenitors from embryonic stem cells", DEVELOPMENT (CAMBRIDGE), vol. 131, no. 11, June 2004 (2004-06-01), pages 2749 - 2762, XP002511712, ISSN: 0950-1991 [Y] 1-54 * the whole document * DOI: http://dx.doi.org/10.1242/DEV.01130 | [Y] WANG LISHENG ET AL: "Endothelial and hematopoietic cell fate of human embryonic stem cells originates from primitive endothelium with hemangioblastic properties", IMMUNITY, CELL PRESS, US LNKD- DOI:10.1016/J.IMMUNI.2004.06.006, vol. 21, no. 1, 1 July 2004 (2004-07-01), pages 31 - 41, XP002484358, ISSN: 1074-7613 [Y] 1-54 * the whole document * DOI: http://dx.doi.org/10.1016/j.immuni.2004.06.006 | [Y] BHATIA MICKIE: "Hematopoiesis from human embryonic stem cells.", ANNALS OF THE NEW YORK ACADEMY OF SCIENCES JUN 2007 LNKD- PUBMED:17332088, vol. 1106, June 2007 (2007-06-01), pages 219 - 222, XP007912752, ISSN: 0077-8923 [Y] 1-54 * figure 1 * DOI: http://dx.doi.org/10.1196/annals.1392.007 | [Y] KENNEDY MARION ET AL: "Development of the hemangioblast defines the onset of hematopoiesis in human ES cell differentiation cultures", BLOOD, AMERICAN SOCIETY OF HEMATOLOGY, US, vol. 109, no. 7, 1 April 2007 (2007-04-01), pages 2679 - 2687, XP002483687, ISSN: 0006-4971 [Y] 1-54 * the whole document * | [Y] PEARSON STELLA ET AL: "The stepwise specification of embryonic stem cells to hematopoietic fate is driven by sequential exposure to Bmp4, activin A, bFGF and VEGF", DEVELOPMENT, COMPANY OF BIOLOGISTS, CAMBRIDGE, GB LNKD- DOI:10.1242/DEV.011767, vol. 135, no. 8, 1 April 2008 (2008-04-01), pages 1525 - 1535, XP008092701, ISSN: 0950-1991 [Y] 1-54 * the whole document * DOI: http://dx.doi.org/10.1242/dev.011767 | [Y] PICK MARJORIE ET AL: "Differentiation of human embryonic stem cells in serum-free medium reveals distinct roles for bone morphogenetic protein 4, vascular endothelial growth factor, stem cell factor, and fibroblast growth factor 2 in hematopoiesis", STEM CELLS, ALPHAMED PRESS LNKD- DOI:10.1634/STEMCELLS.2006-0713, vol. 25, no. 9, 1 September 2007 (2007-09-01), pages 2206 - 2214, XP008092704, ISSN: 1549-4918, [retrieved on 20070607] [Y] 1-54 * the whole document * DOI: http://dx.doi.org/10.1634/stemcells.2006-0713 | [Y] NAKAYAMA NAOKI ET AL: "Vascular endothelial growth factor synergistically enhances bone morphogenetic protein-4-dependent lymphohematopoietic cell generation from embryonic stem cells in vitro", BLOOD, vol. 95, no. 7, 1 April 2000 (2000-04-01), pages 2275 - 2283, XP002221609, ISSN: 0006-4971 [Y] 1-54 * the whole document * | [Y] WANG ZACK Z ET AL: "BMP4 and TGFbeta Differentially Regulate CD34+Progenitor Development in Human Embryonic Stem Cells through SMAD-Dependent Pathway", BLOOD, vol. 112, no. 11, November 2008 (2008-11-01), & 50TH ANNUAL MEETING OF THE AMERICAN- SOCIETY-OF-HEMATOLOGY; SAN FRANCISCO, CA, USA; DECEMBER 06 -09, 2008, pages 328, XP007912753, ISSN: 0006-4971 [Y] 1-54 * the whole document * | [Y] TIAN XINGHUI ET AL: "Differentiation of embryonic stem cells towards hematopoietic cells: progress and pitfalls", CURRENT OPINION IN HEMATOLOGY, RAPID SCIENCE PUBLISHERS, PHILADELPHIA, PA, US, vol. 15, no. 4, 1 July 2008 (2008-07-01), pages 312 - 318, XP009132345, ISSN: 1065-6251 [Y] 1-54 * the whole document * | [Y] NOROL F ET AL: "Ex vivo expanded mobilized peripheral blood CD34+ cells accelerate haematological recovery in a baboon model of autologous transplantation", BRITISH JOURNAL OF HAEMATOLOGY, WILEY-BLACKWELL PUBLISHING LTD, GB LNKD- DOI:10.1046/J.1365-2141.2000.01995.X, vol. 109, 1 January 2000 (2000-01-01), pages 162 - 172, XP003013492, ISSN: 0007-1048 [Y] 1,4-54 * the whole document * DOI: http://dx.doi.org/10.1046/j.1365-2141.2000.01995.x | [Y] YANG SHI ET AL: "[Hematopoietic reconstitution of fresh and cultured cord blood CD34+ cells in NOD/SCID mice]", XI BAO YU FEN ZI MIAN YI XUE ZA ZHI = CHINESE JOURNAL OF CELLULAR AND MOLECULAR IMMUNOLOGY OCT 2008 LNKD- PUBMED:18845075, vol. 24, no. 10, October 2008 (2008-10-01), pages 947 - 949, XP007912757, ISSN: 1007-8738 [Y] 1,4-54 * the whole document * | [Y] FADILAH S A W ET AL: "Cord blood CD34+ cells cultured with FLT3L, stem cell factor, interleukin-6, and IL-3 produce CD11c+CD1a-/c- myeloid dendritic cells.", STEM CELLS AND DEVELOPMENT OCT 2007 LNKD- PUBMED:17999605, vol. 16, no. 5, October 2007 (2007-10-01), pages 849 - 855, XP007912758, ISSN: 1547-3287 [Y] 1,4-54 * the whole document * DOI: http://dx.doi.org/10.1089/scd.2007.0003 | [Y] SHIOZAWA YUSUKE ET AL: "Human Osteoblasts Support Hematopoietic Cell Development in vitro", ACTA HAEMATOLOGICA, S. KARGER, BASEL, CH, vol. 120, no. 3, 28 November 2008 (2008-11-28), pages 134 - 145, XP009132445, ISSN: 0001-5792 [Y] 1,4-54 * the whole document * | [Y] WU M H ET AL: "Optimization of culture conditions to enhance transfection of human CD34+ cells by electroporation", BONE MARROW TRANSPLANTATION, vol. 27, no. 11, 1 June 2001 (2001-06-01), pages 1201 - 1209, XP007913656, ISSN: 0268-3369 [Y] 1,3-54 * the whole document * DOI: http://dx.doi.org/10.1038/sj.bmt.1703054 | [Y] CHUTE J P ET AL: "A comparative study of the cell cycle status and primitive cell adhesion molecule profile of human CD34+ cells cultured in stroma-free versus porcine microvascular endothelial cell cultures.", EXPERIMENTAL HEMATOLOGY FEB 1999 LNKD- PUBMED:10029177, vol. 27, no. 2, February 1999 (1999-02-01), pages 370 - 379, XP000929912, ISSN: 0301-472X [Y] 1-54 * the whole document * DOI: http://dx.doi.org/10.1016/S0301-472X(98)00004-6 | [Y] HUBER TARA L ET AL: "Cooperative effects of growth factors involved in the induction of hematopoietic mesoderm", BLOOD, vol. 92, no. 11, 1 December 1998 (1998-12-01), pages 4128 - 4137, XP007913633, ISSN: 0006-4971 [Y] 2,7-54 * the whole document * | [Y] SLACK J M W ET AL: "MESODERM INDUCTION BY FIBROBLAST GROWTH FACTOR IN EARLY XENOPUS DEVELOPMENT", PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON B BIOLOGICAL SCIENCES, vol. 327, no. 1239, 1990, pages 75 - 84, XP007913634, ISSN: 0962-8436 [Y] 2,7-54 * the whole document * DOI: http://dx.doi.org/10.1098/rstb.1990.0044 | [Y] ZHANG PENGBO ET AL: "Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells", BLOOD, vol. 111, no. 4, February 2008 (2008-02-01), pages 1933 - 1941, XP007913635, ISSN: 0006-4971 [Y] 2,7-54 * Mesoderm induction in human ES cells by BMP-4 and FGF results in cells capable of hematopoietic differentiation.; page 1935, column 1, paragraph 3 * * page 1937, column 1, paragraph 4 - page 1938, column 1, paragraph 2 * DOI: http://dx.doi.org/10.1182/blood-2007-02-074120 | [Y] PURPURA K A ET AL: "Analysis of the temporal and concentration-dependent effects of BMP-4, VEGF, and TPO on development of embryonic stem cell-derived mesoderm and blood progenitors in a defined, serum-free media", EXPERIMENTAL HEMATOLOGY, ELSEVIER INC, US LNKD- DOI:10.1016/J.EXPHEM.2008.04.003, vol. 36, no. 9, 1 September 2008 (2008-09-01), pages 1186 - 1198, XP023976901, ISSN: 0301-472X, [retrieved on 20080611] [Y] 1,4-54 * Effect of TPO on BMP-4/VEGF mouse ES cell cultures.; page 1195; figures 5, 6 * * page 1938, column 1, paragraph 2 * DOI: http://dx.doi.org/10.1016/j.exphem.2008.04.003 | [X] YAMASHITA ET AL: "Differentiation of Arterial, Venous, and Lymphatic Endothelial Cells From Vascular Progenitors", TRENDS IN CARDIOVASCULAR MEDICINE, ELSEVIER SCIENCE, NEW YORK, NY, US LNKD- DOI:10.1016/J.TCM.2007.01.001, vol. 17, no. 2, 7 February 2007 (2007-02-07), pages 59 - 63, XP005878224, ISSN: 1050-1738 [X] 65,66 * the whole document * DOI: http://dx.doi.org/10.1016/j.tcm.2007.01.001 | [Y] MOHAMED AZZA A ET AL: "Ex vivo expansion of stem cells: defining optimum conditions using various cytokines.", LABORATORY HEMATOLOGY : OFFICIAL PUBLICATION OF THE INTERNATIONAL SOCIETY FOR LABORATORY HEMATOLOGY 2006 LNKD- PUBMED:16751136, vol. 12, no. 2, 2006, pages 86 - 93, XP009135584, ISSN: 1080-2924 [Y] 1-54 * the whole document * | by applicant | BASHCY ET AL., TRANSFUSION, vol. 47, no. 11, 2007, pages 2153 - 2160 [Y] 1,4-54 * the whole document * | BHARDWAJ ET AL., NAT. IMMUNOL., vol. 2, 2001, pages 172 - 180 [Y] 1,4-54 * the whole document * | BHATIA ET AL., J. EXP. MED., vol. 189, 1999, pages 1139 - 1148 [Y] 1,4-54 * the whole document * | CHADWICK ET AL., BLOOD, vol. 102, no. 3, 2003, pages 906 - 915 | DAVIDSON; ZON, CURR. TOP DEV. BIOL., vol. 50, 2000, pages 45 - 60 [Y] 1,3-54 * the whole document * | DAVIES ET AL., STRUCTURE, vol. 8, no. 2, 2000, pages 185 - 195 [Y] 1-54 * the whole document * | DREXLER ET AL., GROWTH FACTORS, vol. 22, no. 2, 2004, pages 71 - 3 [Y] 2,7-54 * the whole document * | FADILAH ET AL., STEM CELLS DEV., vol. 16, no. 5, 2007, pages 849 - 856 [Y] 2,7-54 * the whole document * | GAUR ET AL., J. THROMB. HAEMOST., vol. 4, no. 2, 2006, pages 436 - 42 [Y] 2,7-54 * Mesoderm induction in human ES cells by BMP-4 and FGF results in cells capable of hematopoietic differentiation.; page 1935, column 1, paragraph 3 * * page 1937, column 1, paragraph 4 - page 1938, column 1, paragraph 2 * | HANNA ET AL., SCIENCE, vol. 318, no. 5858, 2007, pages 1920 - 1923 [Y] 1,4-54 * Effect of TPO on BMP-4/VEGF mouse ES cell cultures.; page 1195; figures 5, 6 * * page 1938, column 1, paragraph 2 * | HUBER ET AL., BLOOD, vol. 92, 1998, pages 4128 - 4137 [X] 65,66 * the whole document * | IKENOYA ET AL., J. NEUROCHEM, vol. 81, 2002, pages 9 [Y] 1-54 * the whole document * | KAUFMAN ET AL., PROC. NATL. ACAD. SCI. USA, vol. 98, 2001, pages 19 | KISELYOV ET AL., STRUCTURE, vol. 11, no. 6, 2003, pages 691 - 701 | LAPPALAINEN ET AL., CLIN. EXPERIM. ALLERGY, vol. 37, 2007, pages 1404 - 1414 | LIN ET AL., INT. J. MOL. MED., vol. 17, 2006, pages 833 - 839 | LUDWIG ET AL., NATURE BIOTECH., 2006, pages 185 - 187 | LUDWIG ET AL., NATURE METHODS, vol. 3, no. 8, 2006, pages 637 - 646 | MAEKAWA ET AL., SCIENCE, vol. 285, no. 5429, 1999, pages 895 - 8 | MARSHALL ET AL., BLOOD, vol. 96, 2000, pages 1591 - 1593 | NAKAGAWA ET AL., NAT. BIOTECHNOL., vol. 26, no. 1, 2007, pages 101 - 106 | PENG ET AL., CELL BIOLOGY INTERNATIONAL, vol. 32, no. 10, 2008, pages 1265 - 1271 | RATAJCZAK ET AL., BR. J. HAEMATOL., vol. 93, no. 4, 1996, pages 772 - 782 | GENNARO: "Remington: The Science and Practice of Pharmacy, 21st Ed.,", UNIVERSITY OF THE SCIENCES | SASAKI ET AL., PHARMACOL. THER., vol. 93, 2002, pages 225 | SCHERNTHANER ET AL., BLOOD, vol. 98, 2001, pages 3784 - 3792 | SLUKVIN ET AL.: "Directed Production of Specific Blood Lineages from Human Embryonic Stem Cells", ASCI/AAP JOINT MEET. POSTERS, vol. #33, 2007 | TAKAHASHI ET AL., CELL, vol. 131, no. 5, 2007, pages 861 - 872 | TAKAHASHI ET AL., NAT. PROTOC., vol. 2, no. 12, 2007, pages 3081 - 3089 | VODYANIK ET AL., BLOOD, vol. 108, no. 6, 2006, pages 2095 - 105 | WAGEMAKER ET AL., BIOTHERAPY, vol. 2, no. 4, 1990, pages 337 - 345 | WANG ET AL., NATURE BIOTECH., vol. 25, no. 3, 2007, pages 317 - 318 | YAMAMURA ET AL., STEM CELLS, vol. 26, no. 2, 2008, pages 543 - 9 | YU ET AL., SCIENCE, vol. 318, no. 5858, 2007, pages 1917 - 1920 |