EP2419509 - DEVELOPMENT OF FLUORESCENTLY P-LOOP LABELLED KINASES FOR SCREENING OF INHIBITORS [Right-click to bookmark this link] | Status | The application is deemed to be withdrawn Status updated on 02.05.2014 Database last updated on 19.10.2024 | Most recent event Tooltip | 02.05.2014 | Application deemed to be withdrawn | published on 04.06.2014 [2014/23] | Applicant(s) | For all designated states Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Hofgartenstrasse 8 80539 München / DE | [N/P] |
Former [2012/08] | For all designated states Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Hofgartenstrasse 8 80539 München / DE | ||
Former [N/P] | For all designated states Max-Planck-Gesellschaft Zur Förderung Der Wissenschaften E.V. Hofgartenstra e 8 80539 München / DE | Inventor(s) | 01 /
RAUH, Daniel Wasserturmstrasse 16 67549 Worms / DE | 02 /
SIMARD, Jeffrey, Raymond 37 Hillside Avenue Salem MA 01970 / US | [2012/08] | Representative(s) | Vossius & Partner Patentanwälte Rechtsanwälte mbB Siebertstrasse 3 81675 München / DE | [N/P] |
Former [2012/08] | Vossius & Partner Siebertstrasse 4 81675 München / DE | Application number, filing date | 10714279.6 | 19.04.2010 | WO2010EP55129 | Priority number, date | EP20090005492 | 17.04.2009 Original published format: EP 09005492 | US20090170375P | 17.04.2009 Original published format: US 170375 P | [2012/08] | Filing language | EN | Procedural language | EN | Publication | Type: | A1 Application with search report | No.: | WO2010119138 | Date: | 21.10.2010 | Language: | EN | [2010/42] | Type: | A1 Application with search report | No.: | EP2419509 | Date: | 22.02.2012 | Language: | EN | The application published by WIPO in one of the EPO official languages on 21.10.2010 takes the place of the publication of the European patent application. | [2012/08] | Search report(s) | International search report - published on: | EP | 21.10.2010 | Classification | IPC: | C12N9/16, C12Q1/48 | [2012/08] | CPC: |
C12N9/12 (EP,US);
C12Q1/485 (EP,US);
G01N33/531 (EP,US);
G01N2500/00 (EP,US)
| Designated contracting states | AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LI, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR [2012/08] | Title | German: | ENTWICKLUNG VON MIT P-SCHLEIFE FLUORESZENT MARKIERTEN KINASEN FÜR DAS SCREENING VON HEMMERN | [2013/27] | English: | DEVELOPMENT OF FLUORESCENTLY P-LOOP LABELLED KINASES FOR SCREENING OF INHIBITORS | [2012/08] | French: | Développement de kinases étiquetées P-loop à fluorescence pour le criblage des inhibiteurs | [2013/27] |
Former [2012/08] | ENTWICKLUNG VON P-LOOP-FLUORESZENZMARKIERTEN KINASEN ZUM SCREENING VON INHIBITOREN | ||
Former [2012/08] | DÉVELOPPEMENT DE KINASES MARQUÉES EN BOUCLE P PAR FLUORESCENCE POUR UN CRIBLAGE D'INHIBITEURS | Entry into regional phase | 15.11.2011 | National basic fee paid | 15.11.2011 | Designation fee(s) paid | 15.11.2011 | Examination fee paid | Examination procedure | 17.11.2011 | Examination requested [2012/08] | 31.05.2012 | Amendment by applicant (claims and/or description) | 22.08.2012 | Despatch of a communication from the examining division (Time limit: M02) | 09.10.2012 | Reply to a communication from the examining division | 04.02.2013 | Despatch of a communication from the examining division (Time limit: M02) | 12.04.2013 | Reply to a communication from the examining division | 02.08.2013 | Communication of intention to grant the patent | 13.12.2013 | Application deemed to be withdrawn, date of legal effect [2014/23] | 20.01.2014 | Despatch of communication that the application is deemed to be withdrawn, reason: fee for grant / fee for printing not paid in time [2014/23] | Divisional application(s) | The date of the Examining Division's first communication in respect of the earliest application for which a communication has been issued is 22.08.2012 | Fees paid | Renewal fee | 27.03.2012 | Renewal fee patent year 03 | 23.04.2013 | Renewal fee patent year 04 |
Opt-out from the exclusive Tooltip competence of the Unified Patent Court | See the Register of the Unified Patent Court for opt-out data | ||
Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court. | Cited in | International search | [X] - LANGER THOMAS ET AL, "NMR backbone assignment of a protein kinase catalytic domain by a combination of several approaches: application to the catalytic subunit of cAMP-dependent protein kinase", CHEMBIOCHEM - A EUROPEAN JOURNAL OF CHEMICAL BIOLOGY, WILEY VCH, WEINHEIM, DE, (20041105), vol. 5, no. 11, ISSN 1439-4227, pages 1508 - 1516, XP002503169 [X] 1-4,9,11,13 * abstract * DOI: http://dx.doi.org/10.1002/CBIC.200400129 | [X] - JOSEPH R. LAKOWICZ, Principles of fluorescence spectroscopy, SPRINGER, (2006), ISBN 0387312781, XP000918225 [X] 1-4 * see section 13.5.3 wherein alexa 546 labeled kinase is mentionned; page 459 * | [X] - LEVINE H III ET AL, "A MONOCLONAL ANTIBODY AGAINST BRAIN CALMODULIN-DEPENDENT PROTEIN KINASE TYPE II DETECTS PUTATIVE CONFORMATION CHANGES INDUCED BY CALCIUM CALMODULIN", BIOCHEMISTRY, (1988), vol. 27, no. 17, ISSN 0006-2960, pages 6612 - 6617, XP002540825 [X] 1-4 * abstract * DOI: http://dx.doi.org/10.1021/bi00417a060 | [X] - FIRST E A ET AL, "SELECTIVE MODIFICATION OF THE CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE WITH SULFHYDRYL-SPECIFIC FLUORESCENT PROBES", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY, EASTON, PA.; US, (19890101), vol. 28, no. 8, ISSN 0006-2960, pages 3598 - 3605, XP002073139 [X] 1-4 * abstract * DOI: http://dx.doi.org/10.1021/bi00434a067 | [A] - KAPAHI PANKAJ ET AL, "Inhibition of NF-kappaB activation by arsenite through reaction with a critical cysteine in the activation loop of IkappaB kinase", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM, US, vol. 275, no. 46, ISSN 0021-9258, (20001101), pages 36062 - 36066, (20000830), XP002503167 [A] 1-17 * page 36065, column 1, paragraph 2; figure 4 * DOI: http://dx.doi.org/10.1074/JBC.M007204200 | by applicant | - HOLZGRABE; BECHTOLD, DEUTSCHE APOTHEKER ZEITUNG, (2000), vol. 140, no. 8, pages 813 - 823 | - ADRIAN, F. J.; DING, Q.; SIN, T.; VELENTZA, A.; SLOAN, C.; LIU, Y.; ZHANG, G.; HUR, W.; DING, S.; MANLEY, P., NAT CHEM BIOL, (2006), vol. 2, pages 95 - 102 | - ANNIS, D. A.; NAZEF, N.; CHUANG, C. C.; SCOTT, M. P.; NASH, H. M., "A general technique to rank protein-ligand binding affinities and determine allosteric versus direct binding site competition in compound mixtures", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, (2004), vol. 126, pages 15495 - 15503 | - R. T. AIMES; W. HEMMER; S. S. TAYLOR, "Serine-53 at the tip of the glycine-rich loop of cAMP- dependent protein kinase: role in catalysis, P-site specificity, and interaction with inhibitors", BIOCHEMISTRY, (2000), vol. 39, page 8325 | - B. APSEL; J. A. BLAIR; B. GONZALEZ; T. M. NAZIF; M. E. FELDMAN; B. AIZENSTEIN; R. HOFFMAN; R. L. WILLIAMS; K. M. SHOKAT; Z. A. KNI, NAT CHEM BIOL, (2008), vol. 4, page 691 | - C. BACKES; B. ZECH; B. FELBER; B. KLEBL; G. MU))ER, EXPERT OPIN DRUG DISCOVERY, (2008), vol. 3, page 1409 | - C. BACKES; B. ZECH; B. FELBER; B. KLEBL; G. MüLLER, EXPERT OPIN DRUG DISCOVERY, (2008), vol. 3, page 1427 | - BEEBE, J. S.; JANI, J. P.; KNAUTH, E.; GOODWIN, P.; HIGDON, C.; ROSSI, A. M.; EMERSON, E.; FINKELSTEIN, M.; FLOYD, E.; HARRIMAN, S, "Pharmacological characterization of CP-547,632, a novel vascular endothelial growth factor receptor-2 tyrosine kinase inhibitor for cancer therapy", CANCER RES, (2003), vol. 63, pages 7301 - 9 | - J. A. BLAIR; D. RAUH; C. KUNG; C. H. YUN; Q. W. FAN; H. RODE; C. ZHANG; M. J. ECK; W. A. WEISS; K. M. SHOKAT, NAT CHEM BIOL, (2007), vol. 3, page 229 | - CALLEJA, V.; LAGUERRE, M.; PARKER, P. J.; LARIJANI, B., PLOS BIOL, (2009), vol. 7, pages 189 - 200 | - R. A. COPELAND; D. L. POMPLIANO; T. D. MEEK, NAT REV DRUG DISCOV, (2006), vol. 5, page 730 | - DAR, A. C.; LOPEZ, M. S.; SHOKAT, K. M., "Small Molecule Recognition of c-Src via the lmatinib-Binding Conformation", CHEM BIOL, (2008), vol. 15, no. 10, page 1015 | - DELANO, W.L., THE PYMOL MOLECULAR GRAPHICS SYSTEM, (2002), URL: www.pymol.org | - LORIMIER, R. M.; SMITH, J. J.; DWYER, M. A.; LOOGER, L. L.; SALI, K. M.; PAAVOLA, C. D.; RIZK, S. S.; SADIGOV, S.; CONRAD, D. W.;, "Construction of a fluorescent biosensor family", PROTEIN SCI, (2002), vol. 11, pages 2655 - 2675 | - DUMAS, J.; HATOUM-MOKDAD, H.; SIBLEY, R.; RIEDL, B.; SCOTT, W. J.; MONAHAN, M. K.; LOWINGER, T. B.; BRENNAN, C.; NATERO, R.; TURNE, "1-Phenyl-5-pyrazolyl ureas: potent and selective p38 kinase inhibitors", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, (2000), vol. 10, pages 2051 - 2054 | - EMSLEY, P.; COWTAN, K., "Coot: model-building tools for molecular graphics", ACTA CRYSTALLOGR D BIOL CRYSTALLOGR, (2004), vol. 60, page 2126 | - EYERS, P. A.; CHURCHILL, M. E.; MALLER, J. L., "The Aurora A and Aurora B protein kinases: a single amino acid difference controls intrinsic activity and activation by TPX2", CELL CYCLE, (2005), vol. 4, pages 784 - 9 | - M. J. GARNETT; R. MARAIS, CANCER CELL, (2004), vol. 6, page 313 | - GETLIK, M.; GRUTTER, C.; SIMARD, J. R.; KLUTER, S.; RABILLER, M.; RODE, H. B.; ROBUBI, A.; RAUH, D., "Hybrid compound design to overcome the gatekeeper T338M mutation in cSrc", J MED CHEM, (2009), vol. 52, pages 3915 - 26 | - GSCHWIND, A.; FISCHER, O. M.; ULLRICH, A., "The discovery of receptor tyrosine kinases: targets for cancer therapy", NAT REV CANCER, (2004), vol. 4, no. 5, page 361 | - S. K. HANKS; T. HUNTER, FASEB J, (1995), vol. 9, page 576 | - HIBBS, R. E.; TALLEY, T. T.; TAYLOR, P., "Acrylodan-conjugated cysteine side chains reveal conformational state and ligand site locations of the acetylcholine-binding protein", THE JOURNAL OF BIOLOGICAL CHEMISTRY, (2004), vol. 279, pages 28483 - 28491 | - JOHNSON, L. N.; NOBLE, M. E. M.; OWEN, D. J., "Active and inactive protein kinases : structural basis for regulation", CELL, (1996), vol. 85, pages 149 - 158 | - JOHNSON, L. N.; LEWIS, R. E., "Structural basis for control by phosphorylation", CHEM REV., (200108), vol. 101, no. 8, pages 2209 - 42 | - KABSCH, W., "Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constants", J. APPL. CRYST., (1993), vol. 26, page 795 | - KARAMAN, M. W.; HERRGARD, S.; TREIBER, D. K.; GALLANT, P.; ATTERIDGE, C. E.; CAMPBELL, B. T.; CHAN, K. W.; CICERI, P.; DAVIS, M. I, "A quantitative analysis of kinase inhibitor selectivity", NAT BIOTECHNOL, (2008), vol. 26, pages 127 - 32 | - KNIGHTON, D. R.; ZHENG, J. H.; TEN EYCK, L. F.; XUONG, N. H; TAYLOR, S. S.; SOWADSKI, J. M., "Structure of a peptide inhibitor bound to the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase", SCIENCE, (1991), vol. 253, pages 414 - 20 | - LAFONT, V.; ARMSTRONG, A. A.; OHTAKA, H.; KISO, Y.; MARIO AMZEL, L.; FREIRE, E., CHEM BIOL DRUG DES, (2007), vol. 69, pages 413 - 22 | - LASKOWSKI, R. A.; MACARTHUR, M. W.; MOSS, D. S.; THORNTON, J. M., "PROCHECK: a program to check the stereochemical quality of protein structures", J APPL CRYST, (1993), vol. 26, page 283 | - LARKIN, M. A. ET AL., "Clustal W and Clustal X version 2.0", BIOINFORMATICS, (2007), vol. 23, no. 21, page 2947 | - LEVINSON, N. M. ET AL., "A Src-like inactive conformation in the abl tyrosine kinase domain", PLOS BIOL, (2006), vol. 4, no. 5, page 144 | - LIU, Y.; GRAY, N. S., "Rational design of inhibitors that bind to inactive kinase conformation", NAT CHEM BIOL, (2006), vol. 2, no. 7, page 358 | - LIU, C. C.; SCHULTZ, P. G., "Adding new chemistries to the genetic code", ANNU REV BIOCHEM, (2010), vol. 79, pages 15.1 - 15.32 | - M. MAPELLI; L. MASSIMILIANO; C. CROVACE; M. A. SEELIGER; L. H. TSAI; L. MEIJER; A. MUSACCHIO, J MED CHEM, (2005), vol. 48, page 671 | - MICHALCZYK, A. ET AL., "Structural insights into how irreversible inhibitors can overcome drug resistance in EGFR", BIOORG MED CHEM, (2008), vol. 16, no. 7, page 3482 | - MURALI DHAR, T. G.; WROBLESKI, S. T.; LIN, S.; FURCH, J. A.; NIRSCHL, D. S.; FAN, Y.; TODDERUF, G.; PITT, S.; DOWEYKO, A. M.; SACK, "Synthesis and SAR of p38alpha MAP kinase inhibitors based on heterobicydic scaffolds", BIOORG MED CHEM LETT, (2007), vol. 17, pages 5019 - 24 | - MURSHUDOV, G. N.; VAGIN, A. A.; DODSON, E. J., "Refinement of macromolecular structures by the maximum-likelihood method", ACTA CRYSTALLOGR D BIOL CRYSTALLOGR, (1997), vol. 53, page 240 | - B. NAGAR; W. G. BORNMANN; P. PELLICENA; T. SCHINDLER; D. R. VEACH; W. T. MILLER; B. CLARKSON; J. KURIYAN, CANCER RES, (2002), vol. 62, page 4236 | - NIENABER, V. L. ET AL., "Discovering novel ligands for macromolecules using X-ray crystallographic screening", NATURE BIOTECHNOLOGY, (2000), vol. 18, no. 10, page 1105 | - PARGELLIS, C.; TONG, L.; CHURCHILL, L.; CIRILLO, P. F.; GILMORE, T.; GRAHAM, A. G.; GROB, P. M.; HICKEY, E. R.; MOSS, N.; PAV, S., "Inhibition of p38 MAP kinase by utilizing a novel allosteric binding site", NATURE STRUCTURAL BIOLOGY, (2002), vol. 9, pages 268 - 272 | - PATEL, S. B.; CAMERON, P. M.; O'KEEFE, S. J.; FRANTZ-WATTLEY, B.; THOMPSON, J.; O'NEILL, E. A.; TENNIS, T.; LIU, L.; BECKER, J. W., ACTA CRYSTALLOGR D BIOL CRYSTALLOGR, (2009), vol. 65, pages 777 - 85 | - PAWAR, V. G.; SOS, M. L.; RODE, H. B.; RABILLER, M.; HEYNCK, S.; VAN OTTERLO, W. A. L.; THOMAS, R. K.; RAUH, D., "Synthesis and biological evaluation of 4-anilinoquinolines as potent inhibitors of epidermal growth factor receptor", J MED CHEM, (2010), vol. 53, no. 7, pages 2892 - 901 | - "Rapid and Sensitive Sequence Comparison with FASTP and FASTA", W. A PEARSON, Methods in Enzymology, ACADEMIC PRESS, (1990), vol. 183, pages 63 - 98 | - PIERCE AC; HAAR E; BINCH HM; KAY DP; PATEL SR; LI P, J MED CHEM., (20050224), vol. 48, no. 4, pages 1278 - 81 | - READ, R. J., "Pushing the boundaries of molecular replacement with maximum likelihood", ACTA CRYSTALLOGR D, (2001), vol. 57, page 1373 | - REICH, M. ET AL., "GenePattern 2.0", NAT GENET, (2006), vol. 38, no. 5, page 500 | - REGAN, J.; BREITFELDER, S.; CIRILLO, P.; GILMORE, T.; GRAHAM, A. G.; HICKEY, E.; KLAUS, B.; MADWED, J.; MORIAK, M.; MOSS, N., "Pyrazole urea-based inhibitors of p38 MAP kinase: from lead compound to clinical candidate", JOURNAL OF MEDICINAL CHEMISTRY, (2002), vol. 45, pages 2994 - 3008 | - REGAN, J.; PARGELLIS, C. A.; CIRILLO, P. F.; GILMORE, T.; HICKEY, E. R.; PEET, G. W.; PROTO, A.; SWINAMER, A.; MOSS, N., "The kinetics of binding to p38MAP kinase by analogues of BIRB 796", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, (2003), vol. 13, pages 3101 - 3104 | - RICHIERI, G. V.; OGATA, R. T.; KLEINFELD, A. M., "The measurement of free fatty acid concentration with the fluorescent probe ADIFAB: a practical guide for the use of the ADIFAB probe", MOLECULAR AND CELLULAR BIOCHEMISTRY, (1999), vol. 192, pages 87 - 94 | - M. SARASTE; P. R. SIBBALD; A. WITTINGHOFER, TRENDS BIOCHEM SCI, (1990), vol. 15, page 430 | - SCHUTTELKOPF, A. W.; VAN AALTEN, D. M., "PRODRG: a tool for high-throughput crystallography of protein-ligand complexes", ACTA CRYSTALLOGR D BIOL CRYSTALLOGR, (2004), vol. 60, page 1355 | - SULLIVAN, J. E.; HOLDGATE, G. A.; CAMPBELL, D.; TIMMS, D.; GERHARDT, S.; BREED, J.; BREEZE, A. L.; BERMINGHAM, A.; PAUPTIT, R. A.;, "Prevention of MKK6-dependent activation by binding to p38alpha MAP kinase", BIOCHEMISTRY, (2005), vol. 44, pages 16475 - 16490 | - M. A. SEELIGER; B. NAGAR; F. FRANK; X. CAO; M. N. HENDERSON; J. KURIYAN, STRUCTURE, (2007), vol. 15, page 299 | - SHUKER, S. B.; HAJDUK, P. J.; MEADOWS, R. P.; FESIK, S. W., "Discovering high-affinity ligands for proteins: SAR by NMR", SCIENCE, (1996), vol. 274, no. 5292, page 1531 | - J. R. SIMARD; S. KLUTER; C. GRüTTER; M. GETLIK; M. RABILLER; H. B. RODE; D. RAUH, NAT CHEM BIOL, (2009), vol. 5, pages 394 - 396 | - SIMARD, J. R.; GETLIK, M.; GRUTTER, C.; PAWAR, V.; WULFERT, S.; RABILLER, M.; RAUH, D., "Development of a fluorescent-tagged kinase assay system for the detection and characterization of allosteric kinase inhibitors", J AM CHEM SOC, (2009), vol. 131, pages 13286 - 13296 | - SOS, M. L.; RODE, H. B.; HEYNCK, S.; PEIFER, M.; FISCHER, F.; KIUTER, S.; PAWAR, V. G.; REUTER, C.; HEUCKMANN, J.; WEISS, J., "Chemogenomic profiling provides insights into the limited activity of irreversible EGFR Inhibitors in tumor cells expressing the T790M EGFR resistance mutation", CANCER RESEARCH, (2010), vol. 70, no. 3, pages 868 - 74 | - TAMAYO, N; LIAO, L.; GOLDBERG,M.; POWERS, D.; TUDOR, Y-Y; YU, V.; WONG, L. M.; HENKLE, B.; MIDDLETON, S.; SYED, R., "Design and synthesis of potent pyridazine inhibitors of p38 MAP kinase", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, (2005), vol. 15, pages 2409 - 2413 | - TECLE, H.; FERU, F.; LIU, H.; KUHN, C.; RENNIE, G.; MORRIS, M.; SHAO, J.; CHENG, A. C.; GIKUNJU, D.; MIRET, J., "The design, synthesis and potential utility of fluorescence probes that target DFG-out conformation of p38alpha for high throughput screening binding assay", CHEM BIOL, (2009), vol. 74, pages 547 - 59 | - TAYLOR, S. S.; RADZIO-ANDZELM, E., "Three protein kinase structures define a common motif", STRUCTURE, (1994), vol. 2, pages 345 - 355 | - VOGTHERR, M.; SAXENA, K.; HOELDER, S.; GRIMME, S.; BETZ, M.; SCHIEBORR, U.; PESCATORE, B.; ROBIN, M.; DELARBRE, L.; LANGER, T., "NMR characterization of kinase p38 dynamics in free and ligand- bound forms", ANGEWANDTE CHEMIE, (2006), vol. 45, pages 993 - 997 | - YEATMAN, T. J., "A renaissance for SRC", NAT REV CANCER, (2004), vol. 4, no. 6, page 470 | - TOKARSKI, J.S.; NEWITT, J.; CHANG, C.Y.J.; CHENG, J.D.; WITTEKIND, M.; KIEFER, S.E.; KISH, K.; LEE, F.Y.F.; BORZILERRI, R.; LOMBAR, CANCER RES., (2006), vol. 66, pages 5790 - 5797 | - WAN, P. T.; GARNETT, M. J.; ROE, S. M.; LEE, S.; NICULESCU-DUVAZ, D.; GOOD, V. M.; JONES, C. M.; MARSHALL, C. J.; SPRINGER, C. J.;, CELL, (2004), vol. 116, page 855 | - WANG, X; DESTRUMENT, A; TOURNIER, C, "Physiological roles of MKK4 and MKK7: Insights from animal models", BIOCHIMICA ET BIOPHYSICA ACTA, (2007), vol. 1773, pages 1349 - 1357 | - WONG, L.; LIESER, S. A.; MIYASHITA, O.; MILLER, M.; TASKEN, K.; ONUCHIC, J. N.; ADAMS, J. A.; V. L. WOODS; JR.; P. A. JENNINGS, J MOL BIOL, (2005), vol. 351, page 131 | - YEM, A. W.; EPPS, D. E.; MATHEWS, W. R.; GUIDO, D. M.; RICHARD, K. A.; STAITE, N. D.; DEIBEL, M. R., JR., "Site-specific chemical modification of interleukin-1 beta by acrylodan at cysteine 8 and lysine 103", THE JOURNAL OF BIOLOGICAL CHEMISTRY, (1992), vol. 267, pages 3122 - 3128 | - J. ZHANG; P. L. YANG; N. S. GRAY, NAT. REV. CANCER, (2009), vol. 9, page 28 | EP20080013340 | EP20080020341 |