blank Quick help
blank Maintenance news

Scheduled maintenance

Regular maintenance outages:
between 05.00 and 05.15 hrs CET (Monday to Sunday).

Other outages
Availability
Register Forum

2022.02.11

More...
blank News flashes

News flashes

New version of the European Patent Register - SPC information for Unitary Patents.

2024-03-06

More...
blank Related links

Release notes
European Patent Register Assistant
European Patent Register Resource Book
European Patent Register – pocket guide
FAQ - European Patent Register
FAQ - Register Alert
Federated Register (Flyer)
Global Dossier (Flyer)
Register events
Deep link coverage
Federated Register content
The Patent Information Tour
Fair use charter

Extract from the Register of European Patents

EP About this file: EP2862933

EP2862933 - Bidirectional promoter [Right-click to bookmark this link]
StatusNo opposition filed within time limit
Status updated on  10.07.2020
Database last updated on 26.06.2024
FormerThe patent has been granted
Status updated on  02.08.2019
FormerGrant of patent is intended
Status updated on  10.03.2019
FormerExamination is in progress
Status updated on  10.04.2017
Most recent event   Tooltip08.07.2022Lapse of the patent in a contracting state
New state(s): MK		published on 10.08.2022  [2022/32]
Applicant(s)For all designated states
bisy GmbH
Wetzawinkel 20A
8200 Hofstätten an der Raab / AT
[2020/20]
Former [2020/09]For all designated states
bisy e.U.
Wetzawinkel 20A
8200 Hofstätten an der Raab / AT
Former [2020/08]For all designated states
bisy GmbH
Wünschendorf 292
8200 Hofstätten an der Raab / AT
Former [2019/49]For all designated states
bisy e.U.
Wetzawinkel 20A
8200 Hofstätten an der Raab / AT
Former [2019/36]For all designated states
Technische Universität Graz
Rechbauerstraße 12
8010 Graz / AT
For all designated states
ACIB GmbH
Petersgasse 14
8010 Graz / AT
Former [2015/17]For all designated states
Technische Universität Graz
Rechbauerstraße 12
8010 Graz / AT
For all designated states
ACIB GmbH
Petersg. 14
8010 Graz / AT
Inventor(s)01 / Vogl, Thomas
Thalerseestraße 7
8052 Graz / AT
02 / Kickenweiz, Thomas
Friedrichgasse 6
8010 Graz / AT
03 / Sturmberger, Lukas
Moserhofgasse 34/18
8010 Graz / AT
04 / Glieder, Anton
Wetzawinkel 20
8200 Gleisdorf / AT
 [2015/40]
Former [2015/17]01 / Vogl, Thomas
Waltendorfergürtel 6b
8010 Graz / AT
02 / Kickenweiz, Thomas
Friedrichgasse 6
8010 Graz / AT
03 / Sturmberger, Lukas
Moserhofgasse 34/18
8010 Graz / AT
04 / Glieder, Anton
Wetzawinkel 20
8200 Gleisdorf / AT
Representative(s)Gassner, Birgitta, et al
REDL Life Science Patent Attorneys
Donau-City-Straße 11
1220 Wien / AT
[2015/17]
Application number, filing date14175932.407.07.2014
[2015/17]
Priority number, dateEP2013017541605.07.2013         Original published format: EP 13175416
[2015/17]
Filing languageEN
Procedural languageEN
PublicationType: A2 Application without search report 
No.:EP2862933
Date:22.04.2015
Language:EN
[2015/17]
Type: A3 Search report 
No.:EP2862933
Date:22.07.2015
Language:EN
[2015/30]
Type: B1 Patent specification 
No.:EP2862933
Date:04.09.2019
Language:EN
[2019/36]
Type: B8 Corrected title page of specification 
No.:EP2862933
Date:11.12.2019
[2019/50]
Search report(s)(Supplementary) European search report - dispatched on:EP24.06.2015
ClassificationIPC:C12N15/81, C12N15/10, C12Q1/68
[2015/17]
CPC:
C12N15/1086 (EP,US); C12N15/815 (US); C12N15/85 (US);
C12Q1/6897 (EP,US); C12N15/1051 (EP,US)
Designated contracting statesAL,   AT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   LV,   MC,   MK,   MT,   NL,   NO,   PL,   PT,   RO,   RS,   SE,   SI,   SK,   SM,   TR [2016/09]
Former [2015/17]AL,  AT,  BE,  BG,  CH,  CY,  CZ,  DE,  DK,  EE,  ES,  FI,  FR,  GB,  GR,  HR,  HU,  IE,  IS,  IT,  LI,  LT,  LU,  LV,  MC,  MK,  MT,  NL,  NO,  PL,  PT,  RO,  RS,  SE,  SI,  SK,  SM,  TR 
Extension statesBA20.01.2016
ME20.01.2016
TitleGerman:Bidirektionaler Promotor[2015/17]
English:Bidirectional promoter[2015/17]
French:Promoteur bidirectionnel[2015/17]
Examination procedure20.01.2016Examination requested  [2016/09]
22.01.2016Amendment by applicant (claims and/or description)
07.06.2016Despatch of a communication from the examining division (Time limit: M04)
17.10.2016Reply to a communication from the examining division
30.03.2017Despatch of a communication from the examining division (Time limit: M06)
06.10.2017Reply to a communication from the examining division
31.01.2018Despatch of a communication from the examining division (Time limit: M04)
17.05.2018Reply to a communication from the examining division
07.09.2018Despatch of a communication from the examining division (Time limit: M02)
06.11.2018Reply to a communication from the examining division
19.11.2018Despatch of a communication from the examining division (Time limit: M02)
24.01.2019Reply to a communication from the examining division
11.03.2019Communication of intention to grant the patent
26.06.2019Fee for grant paid
26.06.2019Fee for publishing/printing paid
26.06.2019Receipt of the translation of the claim(s)
Opposition(s)05.06.2020No opposition filed within time limit [2020/33]
Fees paidRenewal fee
22.07.2016Renewal fee patent year 03
24.07.2017Renewal fee patent year 04
27.07.2018Renewal fee patent year 05
25.07.2019Renewal fee patent year 06
Opt-out from the exclusive  Tooltip
competence of the Unified
Patent Court		 See the Register of the Unified Patent Court for opt-out data
Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Lapses during opposition  TooltipHU07.07.2014
AL04.09.2019
AT04.09.2019
CY04.09.2019
CZ04.09.2019
EE04.09.2019
ES04.09.2019
FI04.09.2019
HR04.09.2019
IT04.09.2019
LT04.09.2019
LV04.09.2019
MC04.09.2019
MK04.09.2019
MT04.09.2019
PL04.09.2019
RO04.09.2019
RS04.09.2019
SE04.09.2019
SI04.09.2019
SK04.09.2019
SM04.09.2019
TR04.09.2019
BG04.12.2019
NO04.12.2019
GR05.12.2019
IS05.01.2020
PT06.01.2020
[2022/32]
Former [2022/27]HU07.07.2014
AL04.09.2019
AT04.09.2019
CY04.09.2019
CZ04.09.2019
EE04.09.2019
ES04.09.2019
FI04.09.2019
HR04.09.2019
IT04.09.2019
LT04.09.2019
LV04.09.2019
MC04.09.2019
MT04.09.2019
PL04.09.2019
RO04.09.2019
RS04.09.2019
SE04.09.2019
SI04.09.2019
SK04.09.2019
SM04.09.2019
TR04.09.2019
BG04.12.2019
NO04.12.2019
GR05.12.2019
IS05.01.2020
PT06.01.2020
Former [2021/15]AL04.09.2019
AT04.09.2019
CZ04.09.2019
EE04.09.2019
ES04.09.2019
FI04.09.2019
HR04.09.2019
IT04.09.2019
LT04.09.2019
LV04.09.2019
MC04.09.2019
PL04.09.2019
RO04.09.2019
RS04.09.2019
SE04.09.2019
SI04.09.2019
SK04.09.2019
SM04.09.2019
BG04.12.2019
NO04.12.2019
GR05.12.2019
IS05.01.2020
PT06.01.2020
Former [2020/37]AL04.09.2019
AT04.09.2019
CZ04.09.2019
EE04.09.2019
ES04.09.2019
FI04.09.2019
HR04.09.2019
IT04.09.2019
LT04.09.2019
LV04.09.2019
PL04.09.2019
RO04.09.2019
RS04.09.2019
SE04.09.2019
SI04.09.2019
SK04.09.2019
SM04.09.2019
BG04.12.2019
NO04.12.2019
GR05.12.2019
IS05.01.2020
PT06.01.2020
Former [2020/36]AL04.09.2019
AT04.09.2019
CZ04.09.2019
EE04.09.2019
ES04.09.2019
FI04.09.2019
HR04.09.2019
IT04.09.2019
LT04.09.2019
LV04.09.2019
PL04.09.2019
RO04.09.2019
RS04.09.2019
SE04.09.2019
SK04.09.2019
SM04.09.2019
BG04.12.2019
NO04.12.2019
GR05.12.2019
IS05.01.2020
PT06.01.2020
Former [2020/27]AL04.09.2019
AT04.09.2019
CZ04.09.2019
EE04.09.2019
ES04.09.2019
FI04.09.2019
HR04.09.2019
IT04.09.2019
LT04.09.2019
LV04.09.2019
PL04.09.2019
RO04.09.2019
RS04.09.2019
SE04.09.2019
SK04.09.2019
SM04.09.2019
BG04.12.2019
NO04.12.2019
GR05.12.2019
PT06.01.2020
IS24.02.2020
Former [2020/25]AL04.09.2019
AT04.09.2019
CZ04.09.2019
EE04.09.2019
ES04.09.2019
FI04.09.2019
HR04.09.2019
IT04.09.2019
LT04.09.2019
LV04.09.2019
PL04.09.2019
RO04.09.2019
RS04.09.2019
SE04.09.2019
SK04.09.2019
BG04.12.2019
NO04.12.2019
GR05.12.2019
PT06.01.2020
IS24.02.2020
Former [2020/24]AL04.09.2019
AT04.09.2019
EE04.09.2019
ES04.09.2019
FI04.09.2019
HR04.09.2019
IT04.09.2019
LT04.09.2019
LV04.09.2019
PL04.09.2019
RO04.09.2019
RS04.09.2019
SE04.09.2019
BG04.12.2019
NO04.12.2019
GR05.12.2019
PT06.01.2020
IS24.02.2020
Former [2020/23]AL04.09.2019
AT04.09.2019
EE04.09.2019
ES04.09.2019
FI04.09.2019
HR04.09.2019
IT04.09.2019
LT04.09.2019
LV04.09.2019
PL04.09.2019
RO04.09.2019
RS04.09.2019
SE04.09.2019
BG04.12.2019
NO04.12.2019
GR05.12.2019
PT06.01.2020
Former [2020/22]AL04.09.2019
EE04.09.2019
ES04.09.2019
FI04.09.2019
HR04.09.2019
LT04.09.2019
LV04.09.2019
RS04.09.2019
SE04.09.2019
BG04.12.2019
NO04.12.2019
GR05.12.2019
PT06.01.2020
Former [2020/15]AL04.09.2019
ES04.09.2019
FI04.09.2019
HR04.09.2019
LT04.09.2019
LV04.09.2019
RS04.09.2019
SE04.09.2019
BG04.12.2019
NO04.12.2019
GR05.12.2019
Former [2020/11]ES04.09.2019
FI04.09.2019
HR04.09.2019
LT04.09.2019
LV04.09.2019
RS04.09.2019
SE04.09.2019
BG04.12.2019
NO04.12.2019
GR05.12.2019
Former [2020/10]FI04.09.2019
LT04.09.2019
SE04.09.2019
BG04.12.2019
NO04.12.2019
Former [2020/09]FI04.09.2019
LT04.09.2019
NO04.12.2019
Former [2020/08]LT04.09.2019
NO04.12.2019
Documents cited:Search[A]  - WIBERG F C ET AL, "Production of target-specific recombinant human polyclonal antibodies in mammalian cells", BIOTECHNOLOGY AND BIOENGINEERING, WILEY & SONS, HOBOKEN, NJ, US, (20060605), vol. 94, no. 2, doi:10.1002/BIT.20865, ISSN 0006-3592, pages 396 - 405, XP002449594 [A] 1-8,13-15 * abstract *

DOI:   http://dx.doi.org/10.1002/bit.20865
 [IY]  - TRINKLEIN NATHAN D ET AL, "An abundance of bidirectional promoters in the human genome", GENOME RESEARCH, COLD SPRING HARBOR LABORATORY PRESS, WOODBURY, NY, US, (20040101), vol. 14, no. 1, doi:10.1101/GR.1982804, ISSN 1088-9051, pages 62 - 66, XP009126141 [I] 1-3,13,15 * the whole document * [Y] 4-8,13-15

DOI:   http://dx.doi.org/10.1101/gr.1982804
 [YD]  - HARTNER FRANZ S ET AL, "Promoter library designed for fine-tuned gene expression in Pichia pastoris.", NUCLEIC ACIDS RESEARCH JUL 2008, (200807), vol. 36, no. 12, ISSN 1362-4962, pages 1 - 15, XP002733013 [YD] 1-6,13,15 * page 4, column r * * abstract *

DOI:   http://dx.doi.org/10.1093/nar/gkn369
 [YD]  - PARTOW SIAVASH ET AL, "Characterization of different promoters for designing a new expression vector in Saccharomyces cerevisiae.", YEAST (CHICHESTER, ENGLAND) NOV 2010, (201011), vol. 27, no. 11, ISSN 1097-0061, pages 955 - 964, XP002733014 [YD] 1-4,13,15 * page 955, column r * * abstract *

DOI:   http://dx.doi.org/10.1002/yea.1806
 [Y]  - RUTH CLAUDIA ET AL, "Perspectives on synthetic promoters for biocatalysis and biotransformation.", CHEMBIOCHEM : A EUROPEAN JOURNAL OF CHEMICAL BIOLOGY 12 APR 2010, (20100412), vol. 11, no. 6, ISSN 1439-7633, pages 761 - 765, XP002733015 [Y] 1-3,13,15 * figure 3 *

DOI:   http://dx.doi.org/10.1002/cbic.200900761
 [Y]  - ANONYMOUS, "GeneArt Type IIs Assembly kits", (2013), www.lifetechnologies.com, URL: http://www.lifetechnologies.com/content/dam/LifeTech/migration/files/cloning/pdfs.par.0677.file.dat/CO115274%20final.pdf, (20141126), XP002733017 [Y] 7,8,14 * the whole document *
 [Y]  - ENGLER C ET AL, "Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, US, (20090514), vol. 4, no. 5, doi:10.1371/JOURNAL.PONE.0005553, ISSN 1932-6203, pages e5553 - 1, XP002613222 [Y] 7,8,14 * the whole document *

DOI:   http://dx.doi.org/10.1371/journal.pone.0005553
 [A]  - VOGL THOMAS ET AL, "Regulation of Pichia pastoris promoters and its consequences for protein production.", NEW BIOTECHNOLOGY 25 MAY 2013, (20130525), vol. 30, no. 4, ISSN 1876-4347, pages 385 - 404, XP002733016 [A] 1-8,13-15 * the whole document *

DOI:   http://dx.doi.org/10.1016/j.nbt.2012.11.010
Examination   - M C Golding ET AL, "A bidirectional promoter architecture enhances lentiviral transgenesis in embryonic and extraembryonic stem cells", Gene Therapy, GB, (20110310), vol. 18, no. 8, doi:10.1038/gt.2011.26, ISSN 0969-7128, pages 817 - 826, XP055357962

DOI:   http://dx.doi.org/10.1038/gt.2011.26
by applicantUS2013157308
    - METHODS ENZYMOL., (2011), vol. 498, pages 399 - 406
    - ACS SYNTH. BIOL.,, (2014), vol. 3, no. 2, pages 97 - 106
    - GEIER M; BRAUN A; EMMERSTORFER A; PICHLER H; GLIEDER A., "Production of human cytochrome P450 2D6 drug metabolites with recombinant microbes - a comparative study.", BIOTECHNOL J, (2012), pages 1 - 13
    - CHEN M-T; LIN S; SHANDIL ANDREWS D; STADHEIM TA; CHOI B-K., "Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production", MICROB CELL FACT, (2012), vol. 11, page 91
    - GUDIMINCHI RK; GEIER M; GLIEDER A; CAMATTARI A., "Screening for cytochrome P450 expression in Pichia pastoris whole cells by P450-carbon monoxide complex determination.", BIOTECHNOL J, (2013), vol. 8, pages 146 - 52
    - VOGL T; GLIEDER A., "Regulation of Pichia pastoris promoters and its consequences for protein production.", NEW BIOTECHNOL, (2013), vol. 30, pages 385 - 404
    - ST JOHN TP; DAVIS RW., "The organization and transcription of the galactose gene cluster of Saccharomyces", J MOL BIOL, (1981), vol. 152, pages 285 - 315
    - LOHR D; VENKOV P; ZLATANOVA J., "Transcriptional regulation in the yeast GAL gene family: a complex genetic network", FASEB J, (1995), vol. 9, pages 777 - 87
    - MILLER CA; MARTINAT MA; HYMAN LE., "Assessment of aryl hydrocarbon receptor complex interactions using pBEVY plasmids: expressionvectors with bi-directional promoters for use in Saccharomyces cerevisiae", NUCLEIC ACIDS RES, (1998), vol. 26, pages 3577 - 83
    - PARTOW S; SIEWERS V; BJORN S; NIELSEN J; MAURY J., "Characterization of different promoters for designing a new expression vector in Saccharomyces cerevisiae", YEAST, (2010), vol. 27, pages 955 - 64
    - LI A; LIU Z; LI Q; YU L; WANG D; DENG X., "Construction and characterization of bidirectional expression vectors in Saccharomyces cerevisiae", FEMS YEAST RES, (2008), vol. 8, pages 6 - 9
    - ISHIDA C; ARANDA C; VALENZUELA L; RIEGO L; DELUNA A; RECILLAS-TARGA F ET AL., "The UGA3-GLT1 intergenic region constitutes a promoter whose bidirectional nature is determined by chromatin organization in Saccharomyces cerevisiae", MOL MICROBIOL, (2006), vol. 59, pages 1790 - 806
    - XU Z; WEI W; GAGNEUR J; PEROCCHI F; CLAUDER-MÜNSTER S; CAMBLONG J ET AL., "Bidirectional promoters generate pervasive transcription in yeast", NATURE, (2009), vol. 457, pages 1033 - 7
    - NEIL H; MALABAT C; D'AUBENTON-CARAFA Y; XU Z; STEINMETZ LM; JACQUIER A., "Widespread bidirectional promoters are the major source of cryptic transcripts in yeast", NATURE, (2009), vol. 457, pages 1038 - 42
    - XIE M; HE Y; GAN S., "Bidirectionalization of polar promoters in plants", NAT BIOTECHNOL, (2001), vol. 19, pages 677 - 9
    - SAMMARCO MC; GRABCZYK E., "A series of bidirectional tetracycline-inducible promoters provides coordinated protein expression.", ANAL BIOCHEM, (2005), vol. 346, pages 210 - 6
    - BARON U; FREUNDLIEB S; GOSSEN M; BUJARD H., "Co-regulation of two gene activities by tetracycline via a bidirectional promoter", NUCLEIC ACIDS RES, (1995), vol. 23, pages 3605 - 6
    - FUX C; FUSSENEGGER M., "Bidirectional expression units enable streptogramin-adjustable gene expression in mammalian cells", BIOTECHNOL BIOENG, (2003), vol. 83, pages 618 - 25
    - WEBER W; MARTY RR; KELLER B; RIMANN M; KRAMER BP; FUSSENEGGER M., "Versatile macrolide-responsive mammalian expression vectors for multiregulated multigene metabolic engineering.", BIOTECHNOL BIOENG, (2002), vol. 80, pages 691 - 705
    - AMENDOLA M; VENNERI MA; BIFFI A; VIGNA E; NALDINI L., "Coordinate dual-gene transgenesis by lentiviral vectors carrying synthetic bidirectional promoters", NAT BIOTECHNOL, (2005), vol. 23, pages 108 - 16
    - ANDRIANAKI A; SIAPATI EK; HIRATA RK; RUSSELL DW; VASSILOPOULOS G., "Dual transgene expression by foamy virus vectors carrying an endogenous bidirectional promoter", GENE THER, (2010), vol. 17, pages 380 - 8
    - CROOK NC; FREEMAN ES; ALPER HS., "Re-engineering multicloning sites for function and convenience", NUCLEIC ACIDS RES, (2011), vol. 39, page E92
    - STALEY CA; HUANG A; NATTESTAD M; OSHIRO KT; RAY LE; MULYE T ET AL., "Analysis of the 5' untranslated region (5'UTR) of the alcohol oxidase 1 (AOX1) gene in recombinant protein expression in Pichia pastoris", GENE, (2012), vol. 496, pages 118 - 27
    - MEAD DA; PEY NK; HERRNSTADT C; MARCIL RA; SMITH LM., "A universal method for the direct cloning of PCR amplified nucleic acid.", BIOTECHNOLOGY (N Y, (1991), vol. 9, pages 657 - 63
    - RAO B; ZHONG X; WANG Y; WU Q; JIANG Z; MA L., "Efficient vectors for expression cloning of large numbers of PCR fragments in P . pastoris.", YEAST, (2010), pages 285 - 92
    - GIBSON DG; YOUNG L; CHUANG R; VENTER JC; HUTCHISON CA; SMITH HO., "Enzymatic assembly of DNA molecules up to several hundred kilobases.", NAT METHODS, (2009), vol. 6, pages 343 - 5
    - FOSTER TJ; LUNDBLAD V; HANLEY-WAY S; HALLING SM; KLECKNER N., "Three Tn10-associated excision events: relationship to transposition and role of direct and inverted repeats", CELL, (1981), vol. 23, pages 215 - 27
    - EGNER C; BERG DE., "Excision of transposon Tn5 is dependent on the inverted repeats but not on the transposase function of Tn5.", PROC NATL ACAD SCI U S A, (1981), vol. 78, pages 459 - 63
    - HARTNER FS; RUTH C; LANGENEGGER D; JOHNSON SN; HYKA P; LIN-CEREGHINO GP ET AL., "Promoter library designed for fine-tuned gene expression in Pichia pastoris", NUCLEIC ACIDS RES, (2008), vol. 36, page E76
    - NÄÄTSAARI L; MISTLBERGER B; RUTH C; HAJEK T; HARTNER FS; GLIEDER A., "Deletion of the Pichia pastoris KU70 homologue facilitates platform strain generation for gene expression and synthetic biology", PLOS ONE, (2012), vol. 7, page E39720
    - GUERFAL M; RYCKAERT S; JACOBS PP; AMELOOT P; VAN CRAENENBROECK K; DERYCKE R ET AL., "The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteins", MICROB CELL FACT, (2010), vol. 9, pages 49 - 60
    - PRIELHOFER R; MAURER M; KLEIN J; WENGER J; KIZIAK C; GASSER B ET AL., "Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris", MICROB CELL FACT, (2013), vol. 12, page 5
    - DELIC M; MATTANOVICH D; GASSER B., "Repressible promoters -- A novel tool to generate conditional mutants in Pichia pastoris", MICROB CELL FACT, (2013), vol. 12, page 6
    - HARTNER FS; GLIEDER A., "Regulation of methanol utilisation pathway genes in yeasts", MICROB CELL FACT, (2006), vol. 5, pages 39 - 59
    - BERNHARDT R., "Cytochromes P450 as versatile biocatalysts", J BIOTECHNOL, (2006), vol. 124, pages 128 - 45
The EPO accepts no responsibility for the accuracy of data originating from other authorities; in particular, it does not guarantee that it is complete, up to date or fit for specific purposes.