blank Quick help
blank Maintenance news

Scheduled maintenance

Regular maintenance outages:
between 05.00 and 05.15 hrs CET (Monday to Sunday).

Other outages
Availability

2022.02.11

More...
blank News flashes

Italy joins the Federated Register

Click here for more information on the Federated Register.

2025-06-12

More...
blank Related links

Release notes
European Patent Register Assistant
European Patent Register User Manual
European Patent Register – pocket guide
FAQ - European Patent Register
Federated Register (Flyer)
Global Dossier (Flyer)
Register events
Deep link coverage
Federated Register content
The Patent Information Tour
Fair use charter

Extract from the Register of European Patents

EP About this file: EP2971171

EP2971171 - MULTIPLEX METHYLATION-SPECIFIC AMPLIFICATION METHODS [Right-click to bookmark this link]
Former [2016/03]MULTIPLEX METHYLATION-SPECIFIC AMPLIFICATION SYSTEMS AND METHODS
[2019/23]
StatusThe application is deemed to be withdrawn
Status updated on  21.02.2020
Database last updated on 11.04.2026
FormerGrant of patent is intended
Status updated on  21.05.2019
FormerExamination is in progress
Status updated on  08.09.2017
Most recent event   Tooltip21.02.2020Application deemed to be withdrawnpublished on 25.03.2020  [2020/13]
Applicant(s)For all designated states
Abbott Molecular Inc.
1300 E. Touhy Avenue
Des Plaines, IL 60018 / US
[2016/03]
Inventor(s)01 / GRANADOS, Edward
1300 E. Touhy Avenue
Des Plaines, Illinois 60018 / US
 [2016/03]
Representative(s)Chapman, Desmond Mark
Carpmaels & Ransford LLP
One Southampton Row
London WC1B 5HA / GB
[2016/03]
Application number, filing date14773416.413.03.2014
[2016/03]
WO2014US25854
Priority number, dateUS201361782370P14.03.2013         Original published format: US 201361782370 P
[2016/03]
Filing languageEN
Procedural languageEN
PublicationType: A1 Application with search report
No.:WO2014160117
Date:02.10.2014
Language:EN
[2014/40]
Type: A1 Application with search report 
No.:EP2971171
Date:20.01.2016
Language:EN
The application published by WIPO in one of the EPO official languages on 02.10.2014 takes the place of the publication of the European patent application.
[2016/03]
Search report(s)International search report - published on:US02.10.2014
(Supplementary) European search report - dispatched on:EP06.10.2016
ClassificationIPC:C12Q1/6858, C12Q1/6886
[2019/23]
CPC:
C12Q1/6858 (EP,US); C12Q1/6886 (EP,US); C12Q2600/154 (EP,US)
C-Set:
C12Q1/6858, C12Q2523/125, C12Q2537/143 (EP,US)
Former IPC [2016/03]C12Q1/68
Designated contracting statesAL,   AT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   LV,   MC,   MK,   MT,   NL,   NO,   PL,   PT,   RO,   RS,   SE,   SI,   SK,   SM,   TR [2016/03]
TitleGerman:MULTIPLEX-METHYLIERUNGS-SPEZIFISCHE AMPLIFIZIERUNGSVERFAHREN[2019/23]
English:MULTIPLEX METHYLATION-SPECIFIC AMPLIFICATION METHODS[2019/23]
French:PROCÉDÉS D'AMPLIFICATION MULTIPLEXE SPÉCIFIQUES DE LA MÉTHYLATION[2019/23]
Former [2016/03]MULTIPLEX-METHYLIERUNGS-SPEZIFISCHE VERSTÄRKERSYSTEME UND -VERFAHREN
Former [2016/03]MULTIPLEX METHYLATION-SPECIFIC AMPLIFICATION SYSTEMS AND METHODS
Former [2016/03]SYSTÈMES ET PROCÉDÉS D'AMPLIFICATION MULTIPLEXE SPÉCIFIQUES DE LA MÉTHYLATION
Entry into regional phase24.09.2015National basic fee paid 
24.09.2015Search fee paid 
24.09.2015Designation fee(s) paid 
24.09.2015Examination fee paid 
Examination procedure24.09.2015Examination requested  [2016/03]
03.05.2017Amendment by applicant (claims and/or description)
08.09.2017Despatch of a communication from the examining division (Time limit: M04)
12.01.2018Reply to a communication from the examining division
08.05.2018Despatch of a communication from the examining division (Time limit: M04)
18.09.2018Reply to a communication from the examining division
14.01.2019Despatch of a communication from the examining division (Time limit: M02)
25.03.2019Reply to a communication from the examining division
22.05.2019Communication of intention to grant the patent
02.10.2019Application deemed to be withdrawn, date of legal effect  [2020/13]
08.11.2019Despatch of communication that the application is deemed to be withdrawn, reason: fee for grant / fee for printing not paid in time  [2020/13]
Divisional application(s)The date of the Examining Division's first communication in respect of the earliest application for which a communication has been issued is  08.09.2017
Fees paidRenewal fee
08.03.2016Renewal fee patent year 03
07.03.2017Renewal fee patent year 04
08.03.2018Renewal fee patent year 05
08.03.2019Renewal fee patent year 06
Opt-out from the exclusive  Tooltip
competence of the Unified
Patent Court		 See the Register of the Unified Patent Court for opt-out data
Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Documents cited:Search[XY] WO2007032748  (AGENCY SCIENCE TECH & RES et al.) [X] 1-5,7-11,15 * pages 3-8; claims 1-23,26,29,32,35,38,44 *[Y] 12-14
 [X] US2008254474  (LAIRD PETER W et al.) [X] 1-6,8,9 * paragraphs [0009] , [0015] , [0021] , [0041] , [0054]; figure 5; example 6; claims 3,4,16,23 *
 [XY] WO0142493  (EPIGENOMICS AG et al.) [X] 1-5,7-11,15 * pages 25-26; claims 1-7, 27 *[Y] 12-14
 [XY] DE10215770  (GIESING MICHAEL et al.) [X] 1-5,7-10 * paragraphs [0097] , [0098] , [0106] - [0114]; figure 5 *[Y] 11-15
 [XY]   SALAH AZZI ET AL: "Allele-specific methylated multiplex real-time quantitative PCR (ASMM RTQ-PCR), a powerful method for diagnosing loss of imprinting of the 11p15 region in Russell Silver and Beckwith Wiedemann syndromes", HUMAN MUTATION, vol. 32, no. 2, 28 February 2011 (2011-02-28), US, pages 249 - 258, XP055305370, ISSN: 1059-7794, DOI: 10.1002/humu.21403 [X] 1-10 * page 252; figure 3; table 1 *[Y] 11-15

DOI:   http://dx.doi.org/10.1002/humu.21403
 [XY]   M. J. FACKLER: "Quantitative Multiplex Methylation-Specific PCR Assay for the Detection of Promoter Hypermethylation in Multiple Genes in Breast Cancer", CANCER RESEARCH, vol. 64, no. 13, 1 July 2004 (2004-07-01), pages 4442 - 4452, XP055069915, ISSN: 0008-5472, DOI: 10.1158/0008-5472.CAN-03-3341 [X] 1-5,7-10 * page 4443, column 2, paragraphs 1-3; figure 1; tables 1,2 * * the whole document *[Y] 11-15

DOI:   http://dx.doi.org/10.1158/0008-5472.CAN-03-3341
 [XY]   HE Q ET AL: "Development of a multiplex MethyLight assay for the detection of multigene methylation in human colorectal cancer", CANCER GENETICS AND CYTOGENETICS, ELSEVIER SCIENCE PUBLISHING, NEW YORK, NY, US, vol. 202, no. 1, 1 October 2010 (2010-10-01), pages 1 - 10, XP027246507, ISSN: 0165-4608, [retrieved on 20100827] [X] 1-7,9,10 * page 2, column 2, paragraph 4 - page 4; figure 3; table 2 *[Y] 11-15
International search[X] US2011091882  (GRANADOS EDWARD N et al.) [X]
 [X] US2006110741  (ASAI RYOICHI et al.) [X]
 [A] US2005239101  (SUKUMAR SARASWATI et al.) [A]
 [A] US2010009365  (LAIRD PETER W et al.) [A]
 [A]   DAHL ET AL.: "A ligation assay for multiplex analysis of CpG methylation using bisulfite-treated DNA.", NUCLEIC ACIDS RES., vol. 35, no. 21, 12 November 2007 (2007-11-12), pages 1 - 8, XP055282868 [A]

DOI:   http://dx.doi.org/10.1093/nar/gkm984
by applicantWO2007032748
 WO9500669
 WO9928498
 WO9746705
 WO9515373
 WO9745560
 US5786146
 US4683195
 US4683202
 US4800159
 US4965188
 US5480784
 US5399491
 US5824518
 US2006046265
 US5270184
 US5455166
 EP0684315
 US5130238
 WO0018957
 US7972820
 US7790418
 US5641658
 US6303305
 US6541205
 US5710029
 US6534274
 US5925517
 US6150097
 US5928862
 US2005042638
 US5814447
 US6706162
 US8043493
 US7108974
 US8017743
 US8017322
 WO2006084132
 US5750341
 US6306597
 US6432360
 US6485944
 US6511803
 US2005130173
 US6787308
 US6833246
 US5695934
 US5714330
 US6210891
 US6258568
 US7115400
 US6969488
 US5912148
 US6130073
 US7169560
 US7282337
 US7482120
 US7501245
 US6818395
 US6911345
 US2009026082
 US2009127589
 US2010301398
 US2010197507
 US2010188073
 US2010137143
 US2009035777
 US7329492
 US67195608
 US78116607
 US5283174
   JAENISCH; BIRD, NATURE GENETICS, vol. 33, 2003, pages 245
   DODGE ET AL., GENE, vol. 289, no. 1-2, 2002, pages 41 - 48
   HAINES ET AL., DEVELOPMENTAL BIOLOGY, vol. 240, no. 2, 2001, pages 585 - 598
   MILLER; SWEATT, NEURON, vol. 53, no. 6, 15 March 2007 (2007-03-15), pages 857 - 869
   POWELL; DEVIN, NEW SCIENTIST, 2008
   TUCKER, NEURON, vol. 30, no. 3, 2001, pages 649 - 52
   AZZI ET AL., HUMAN MUTATION, vol. 32, 2011, pages 249 - 258
   REIN, T.; DEPAMPHILIS, M. L.; ZORBAS, H., NUCLEIC ACIDS RES., vol. 26, 1998, pages 2255
   OLEK, A.; WALTER, J., NAT GENET., vol. 17, 1997, pages 275 - 276
   GONZALGO, M. L.; JONES, P. A., NUCL. ACIDS RES., vol. 25, 1997, pages 2529 - 2531
   XIONG, Z.; LAIRD, P. W., NUCL. ACIDS. RES., vol. 25, 1997, pages 2532 - 2534
   XIONG, Z.; LAIRD, P. W., NUCL. ACIDS RES., vol. 25, 1997, pages 2532
   GONZALGO, M. L.; JONES, P. A., NUCL. ACIDS RES., vol. 25, 1997, pages 2529
   GRIGG, S.; CLARK, S., BIOASSAYS, vol. 16, 1994, pages 431
   ZESCHNIK, M. ET AL., HUMAN MOLECULAR GENETICS, vol. 6, 1997, pages 387
   TEIL, R. ET AL., NUCL. ACIDS RES., vol. 22, 1994, pages 695
   MARTIN, V. ET AL., GENE, vol. 157, 1995, pages 261
   LOMBAERTS, M. ET AL., BRITISH JOURNAL OF CANCER, vol. 94, 2006, pages 661 - 671
   YOSHIURA, K. ET AL., PROC. NATL. ACAD. SCI., vol. 92, 1995, pages 7416 - 7419
   LIND, G. E. ET AL., MOLECULAR CANCER, vol. 3, 2004, pages 28
   KUMAGAI, T. ET AL., INT. J. CANCER, vol. 121, 2007, pages 656 - 665
   HENNIG, G. ET AL., J. BIOL. CHEM., vol. 271, no. l, 1996, pages 595 - 602
   MARCHEVSKY, A. M. ET AL., JOURNAL OF MOLECULAR DIAGNOSTICS, vol. 6, 2004, pages 28 - 36
   REINHOLD, W. C. ET AL., MOL. CANCER. THER., vol. 6, 2007, pages 391 - 403
   HU, X-C. ET AL., LIFE SCIENCES, vol. 71, 2002, pages 1397 - 1404
   NAKATA, S. ET AL., CANCER, vol. 106, no. 10, 2006, pages 2190 - 2199
   HERMAN ET AL., PROC. NATL. ACAD. SCI. USA, vol. 93, 1996, pages 9821 - 9826
   EADS ET AL., CANCER RES., vol. 59, 1999, pages 2302 - 2306
   MULLIS ET AL., METH. ENZYMOL., vol. 155, 1987, pages 335
   MURAKAWA ET AL., DNA, vol. 7, 1988, pages 287
   WEISS, R., SCIENCE, vol. 254, 1991, pages 1292
   WALKER, G. ET AL., PROC. NATL. ACAD. SCI. USA, vol. 89, 1992, pages 392 - 396
   LIZARDI ET AL., BIOTECHNOL., vol. 6, 1988, pages 1197
   KWOH ET AL., PROC. NATL. ACAD. SCI. USA, vol. 86, 1989, pages 1173
   GUATELLI ET AL., PROC. NATL. ACAD. SCI. USA, vol. 87, 1990, pages 1874
   PERSING, DAVID H. ET AL.: "Diagnostic Medical Microbiology: Principles and Applications", 1993, AMERICAN SOCIETY FOR MICROBIOLOGY, article "In Vitro Nucleic Acid Amplification Techniques", pages: 51 - 87
   ADESSI ET AL., NUCLEIC ACIDS RESEARCH, vol. 28, no. 20, 2000, pages E87
   MITRA; CHURCH, NUCLEIC ACID RESEARCH, vol. 27, 15 December 1999 (1999-12-15), pages e34
   LIZARDI ET AL., NATURE GENETICS, vol. 19, July 1998 (1998-07-01), pages 225
   LEAMON ET AL., ELECTROPHORESIS, vol. 24, November 2003 (2003-11-01), pages 3769
   DRESSMAN ET AL., PNAS, vol. 100, 22 July 2003 (2003-07-22), pages 8817
   PERLER F. B. ET AL., ADV. PROTEIN CHEM., vol. 48, 1996, pages 377 - 435
   THUM O ET AL., ANGEW. CHEM. INT. ED., vol. 40, no. 21, 2001, pages 3990 - 3993
   GENOMICS, vol. 92, 2008, pages 255
   BIRREN ET AL.: "Genome Analysis: Analyzing DNA", vol. 1, COLD SPRING HARBOR
   MITRA ET AL., ANALYTICAL BIOCHEMISTRY, vol. 320, 2003, pages 55 - 65
   SHENDURE ET AL., SCIENCE, vol. 309, 2005, pages 1728 - 1732
   MARGULIES ET AL., NATURE, vol. 437, 2005, pages 376 - 380
   BENNETT ET AL., PHARMACOGENOMICS, vol. 6, 2005, pages 373 - 382
   BRENNER ET AL., NAT. BIOTECHNOL., vol. 18, 2000, pages 630 - 634
   ADESSI ET AL., NUCLEIC ACID RES., vol. 28, 2000, pages E87
   VOELKERDING ET AL., CLINICAL CHEM., vol. 55, 2009, pages 641 - 658
   MACLEAN ET AL., NATURE REV. MICROBIOL., vol. 7, pages 287 - 296
   ASTIER ET AL., J. AM. CHEM. SOC., vol. 128, no. 5, 8 February 2006 (2006-02-08), pages 1705 - 10
   SCIENCE, vol. 327, no. 5970, 2010, pages 1190
   VOELKERDING ET AL., CLINICAL CHEM., vol. 55, 2009, pages 641 - 58
   NORMAN C. NELSON ET AL.: "Nonisotopic Probing, Blotting, and Sequencing", 1995
The EPO accepts no responsibility for the accuracy of data originating from other authorities; in particular, it does not guarantee that it is complete, up to date or fit for specific purposes.