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Extract from the Register of European Patents

EP About this file: EP3061808

EP3061808 - CULTURE MEDIUM FOR EPITHELIAL STEM CELLS AND ORGANOIDS COMPRISING SAID STEM CELLS [Right-click to bookmark this link]
StatusNo opposition filed within time limit
Status updated on  18.06.2021
Database last updated on 02.09.2024
FormerThe patent has been granted
Status updated on  10.07.2020
FormerGrant of patent is intended
Status updated on  20.02.2020
FormerExamination is in progress
Status updated on  10.11.2017
FormerRequest for examination was made
Status updated on  28.04.2017
Most recent event   Tooltip27.04.2024Lapse of the patent in a contracting state
New state(s): MK		published on 29.05.2024  [2024/22]
Applicant(s)For all designated states
Koninklijke Nederlandse Akademie van Wetenschappen
Kloveniersburgwal 29
1011 JV Amsterdam / NL
[2016/35]
Inventor(s)01 / SATO, Toshiro
Hubrecht Institute
Uppsalalaan 8
3584 CT Utrecht / NL
02 / CLEVERS, Johannes, Carolus
Ruysdaellaan 7
3712 AP Huis ter Heide / NL
03 / HUCH ORTEGA, Meritxell
Willem Barentszstraat 25II
3572 PB Utrecht / NL
 [2016/36]
Former [2016/35]01 / SATO, Toshiro
Langestraat 32a
1211 GZ Hilversum / NL
02 / CLEVERS, Johannes, Carolus
Ruysdaellaan 7
3712 AP Huis ter Heide / NL
03 / HUCH ORTEGA, Meritxell
Willem Barentszstraat 25II
3572 PB Utrecht / NL
Representative(s)Carpmaels & Ransford LLP
One Southampton Row
London WC1B 5HA / GB
[2016/35]
Application number, filing date16151949.103.02.2010
[2016/35]
Priority number, dateEP2009015197003.02.2009         Original published format: EP 09151970
US20090149622P03.02.2009         Original published format: US 149622 P
EP2009017183130.09.2009         Original published format: EP 09171831
[2016/35]
Filing languageEN
Procedural languageEN
PublicationType: A2 Application without search report 
No.:EP3061808
Date:31.08.2016
Language:EN
[2016/35]
Type: A3 Search report 
No.:EP3061808
Date:19.10.2016
Language:EN
[2016/42]
Type: B1 Patent specification 
No.:EP3061808
Date:12.08.2020
Language:EN
[2020/33]
Search report(s)(Supplementary) European search report - dispatched on:EP20.09.2016
ClassificationIPC:C12N5/071
[2016/35]
CPC:
C12N5/0677 (EP,KR,US); C12N1/38 (KR); C12N5/0676 (KR,US);
A61P1/04 (EP); A61P3/10 (EP); A61P43/00 (EP);
C12N5/0607 (KR); C12N5/0679 (EP,KR,US); C12N2501/11 (EP,KR,US);
C12N2501/117 (EP,KR,US); C12N2501/155 (EP,KR,US); C12N2501/415 (EP,KR,US);
C12N2501/70 (EP,KR,US); C12N2533/90 (EP,KR,US) (-)
Designated contracting statesAT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   LV,   MC,   MK,   MT,   NL,   NO,   PL,   PT,   RO,   SE,   SI,   SK,   SM,   TR [2017/22]
Former [2016/35]AT,  BE,  BG,  CH,  CY,  CZ,  DE,  DK,  EE,  ES,  FI,  FR,  GB,  GR,  HR,  HU,  IE,  IS,  IT,  LI,  LT,  LU,  LV,  MC,  MK,  MT,  NL,  NO,  PL,  PT,  RO,  SE,  SI,  SK,  SM,  TR 
Extension statesAL19.04.2017
BA19.04.2017
RS19.04.2017
TitleGerman:NÄHRMEDIUM FÜR EPITHELSTAMMZELLEN UND ORGANELLE MIT DEN BESAGTEN STAMMZELLEN[2016/35]
English:CULTURE MEDIUM FOR EPITHELIAL STEM CELLS AND ORGANOIDS COMPRISING SAID STEM CELLS[2016/35]
French:MILIEU DE CULTURE DE CELLULES SOUCHES ÉPITHÉLIALES ET ORGANOÏDES COMPRENANT LESDITES CELLULES SOUCHES[2016/35]
Examination procedure19.01.2016Date on which the examining division has become responsible
19.04.2017Amendment by applicant (claims and/or description)
19.04.2017Examination requested  [2017/22]
14.11.2017Despatch of a communication from the examining division (Time limit: M06)
20.06.2018Despatch of communication that the application is deemed to be withdrawn, reason: reply to the communication from the examining division not received in time
05.07.2018Reply to a communication from the examining division
26.02.2019Despatch of a communication from the examining division (Time limit: M04)
31.05.2019Reply to a communication from the examining division
29.07.2019Despatch of a communication from the examining division (Time limit: M04)
10.09.2019Reply to a communication from the examining division
22.10.2019Despatch of a communication from the examining division (Time limit: M04)
13.12.2019Reply to a communication from the examining division
21.02.2020Communication of intention to grant the patent
02.07.2020Fee for grant paid
02.07.2020Fee for publishing/printing paid
02.07.2020Receipt of the translation of the claim(s)
Parent application(s)   TooltipEP10703131.2  / EP2393917
Divisional application(s)EP18182285.9  / EP3441458
EP23176802.9  / EP4253529
Opposition(s)14.05.2021No opposition filed within time limit [2021/29]
Request for further processing for:The application is deemed to be withdrawn due to failure to reply to the examination report
05.07.2018Request for further processing filed
05.07.2018Full payment received (date of receipt of payment)
Request granted
17.07.2018Decision despatched
Fees paidRenewal fee
19.01.2016Renewal fee patent year 03
19.01.2016Renewal fee patent year 04
19.01.2016Renewal fee patent year 05
19.01.2016Renewal fee patent year 06
19.01.2016Renewal fee patent year 07
10.02.2017Renewal fee patent year 08
14.02.2018Renewal fee patent year 09
13.02.2019Renewal fee patent year 10
13.02.2020Renewal fee patent year 11
Opt-out from the exclusive  Tooltip
competence of the Unified
Patent Court		 See the Register of the Unified Patent Court for opt-out data
Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Lapses during opposition  TooltipCY12.08.2020
EE12.08.2020
LT12.08.2020
MC12.08.2020
MK12.08.2020
RO12.08.2020
SI12.08.2020
SM12.08.2020
BG12.11.2020
[2024/22]
Former [2023/24]CY12.08.2020
EE12.08.2020
LT12.08.2020
MC12.08.2020
RO12.08.2020
SI12.08.2020
SM12.08.2020
BG12.11.2020
Former [2021/41]EE12.08.2020
LT12.08.2020
MC12.08.2020
RO12.08.2020
SI12.08.2020
SM12.08.2020
BG12.11.2020
Former [2021/36]EE12.08.2020
LT12.08.2020
RO12.08.2020
SI12.08.2020
SM12.08.2020
BG12.11.2020
Former [2021/23]EE12.08.2020
LT12.08.2020
RO12.08.2020
SM12.08.2020
BG12.11.2020
Former [2021/22]EE12.08.2020
LT12.08.2020
RO12.08.2020
BG12.11.2020
Former [2021/10]LT12.08.2020
BG12.11.2020
Former [2021/07]LT12.08.2020
Documents cited:Search[A]WO2007149182  (GERON CORP [US], et al) [A] 1-6 * example 1 *;
 [IA]WO2008101215  (VARIAN MED SYS TECH INC [US], et al) [I] 7-11 * column 70; claims 14,48 * [A] 1-6;
 [XAI]  - ABUD H E ET AL, "Growth of intestinal epithelium in organ culture is dependent on EGF signalling", EXPERIMENTAL CELL RESEARCH, ACADEMIC PRESS, US, (20050215), vol. 303, no. 2, ISSN 0014-4827, pages 252 - 262, XP004709265 [X] 18 * figures 3-5 * [A] 1-6,13,15-17,19 [I] 20,21

DOI:   http://dx.doi.org/10.1016/j.yexcr.2004.10.006
 [XI]  - BOOTH C ET AL, "THE ISOLATION AND CULTURE OF ADULT MOUSE COLONIC EPITHELIUM", EPITHELIAL CELL BIOLOGY, SPRINGER INTERNATIONAL, BERLIN, DE, (19950101), vol. 4, no. 2, ISSN 0940-9912, pages 76 - 86, XP009006497 [X] 18 * page 82; figures 3, 4, 7 * [I] 20,21
 [XI]  - MONTESANO R ET AL, "COLLAGEN MATRIX PROMOTES REORGANIZATION OF PANCREATIC ENDOCRINE CELL MONO LAYERS INTO ISLET-LIKE ORGANOIDS", JOURNAL OF CELL BIOLOGY, (1983), vol. 97, no. 3, ISSN 0021-9525, pages 935 - 939, XP002537542 [X] 18 * figures 4,5 * [I] 20,21

DOI:   http://dx.doi.org/10.1083/jcb.97.3.935
 [A]  - JIANG J ET AL, "Generation of insulin-producing islet-like clusters from human embryonic stem cells", STEM CELLS, ALPHAMED PRESS, DAYTON, OH, US, (20070801), vol. 25, no. 8, ISSN 1066-5099, pages 1940 - 1953, XP008084029 [A] 1-6 * figures 1, 3, 4, 7 *
 [A]  - BJERKNES MATTHEW ET AL, "Intestinal epithelial stem cells and progenitors", METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC, SAN DIEGO, CA, US, (20060101), vol. 419, ISSN 0076-6879, pages 337 - 383, XP009101698 [A] 1-6 * the whole document *

DOI:   http://dx.doi.org/10.1016/S0076-6879(06)19014-X
 [A]  - KIM D ET AL, "IN VIVO FUNCTIONING AND TRANSPLANTABLE MATURE PANCREATIC ISLET-LIKE CELL CLUSTERS DIFFERENTIATED FROM EMBRYONIC STEM CELL", PANCREAS, RAVEN PRESS, NEW YORK, NY, US, (20030801), vol. 27, no. 2, ISSN 0885-3177, pages E34 - E41, XP009063178 [A] 1-6 * figure 3 *

DOI:   http://dx.doi.org/10.1097/00006676-200308000-00021
 [A]  - LIU Y ET AL, "A novel chemical-defined medium with bFGF and N2B27 supplements supports undifferentiated growth in human embryonic stem cells", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 346, no. 1, doi:10.1016/J.BBRC.2006.05.086, ISSN 0006-291X, (20060721), pages 131 - 139, (20060721), XP024925297 [A] 1-21 * the whole document *

DOI:   http://dx.doi.org/10.1016/j.bbrc.2006.05.086
 [T]  - SATO TOSHIRO ET AL, "Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche.", NATURE 14 MAY 2009, (20090514), vol. 459, no. 7244, ISSN 1476-4687, pages 262 - 265, XP002529277 [T] * the whole document *

DOI:   http://dx.doi.org/10.1038/NATURE07935
by applicant   - GREGORIEFF; CLEVERS, GENES DEV, (2005), vol. 19, pages 877 - 90
    - B JERKNES; CHENG, GASTROENTEROLOGY, (1999), vol. 116, pages 7 - 14
    - BARKER ET AL., NATURE, (2007), vol. 449, pages 1003 - 1007
    - BJERKNES; CHENG, METHODS ENZYMOL, (2006), vol. 419, pages 337 - 83
    - SAHA ET AL., CURR OPIN CHEM BIOL., (2007), vol. 11, no. 4, pages 381 - 387
    - SAHA ET AL., BIOPHYSICAL JOURNAL, (2008), vol. 95, pages 4426 - 4438
    - LITTLE ET AL., CHEM. REV, (2008), vol. 108, pages 1787 - 1796
    - ZILBERBERG ET AL., BMC CELL BIOL., (2007), vol. 8, page 41
    - KORINEK ET AL., SCIENCE, (1997), vol. 275, pages 1784 - 1787
    - KIRIKOSHI H ET AL., BIOCHEM BIOPHYS RES COM, (2001), vol. 283, pages 798 - 805
    - KESTUTIS PLANUTIS ET AL., BMC CELL BIOL., (2007), vol. 8, page 12
    - LIU ET AL., ANGEW CHEM INT ED ENGL., (2005), vol. 44, pages 1987 - 90
    - LEOST, M. ET AL., EUR. J. BIOCHEM., (2000), vol. 267, pages 5983 - 5994
    - MEIJER, L. ET AL., CHEM. BIOL., (2003), vol. 10, pages 1255 - 1266
    - MEIJER ET AL., TRENDS IN PHARMACOLOGICAL SCIENCES, (2004), vol. 25, pages 471 - 480
    - LIAO ET AL., ENDOCRINOLOGY, (2004), vol. 145, no. 6, pages 2941 - 9
    - DONTU ET AL., BREAST CANCER RES, (2004), vol. 6, pages R605 - R615
    - HSIEH ET AL., MOL. CELL. BIOL., (1996), vol. 16, pages 952 - 959
    - OOTANI A; TODA S; FUJIMOTO K; SUGIHARA H, AM J PATHOL., (200306), vol. 162, no. 6, pages 1905 - 12
    - WILLERT K; BROWN JD; DANENBERG E; DUNCAN AW; WEISSMAN IL; REYA T; YATES JR 3RD; NUSSE R, NATURE, (20030522), vol. 423, no. 6938, pages 448 - 52
    - BARKER N; VAN ES JH; KUIPERS J; KUJALA P; VAN DEN BORN M; COZIJNSEN M; HAEGEBARTH A; KORVING J; BEGTHEL H; PETERS PJ, NATURE, (2007), vol. 449, pages 1003 - 7
    - SUZUKI ET AL., DIABETES, (2004), vol. 53
    - TRAUTMANN B ET AL., PANCREAS, vol. 8, pages 248 - 254
    - ABE K; WATANABE S., "Apoptosis of mouse pancreatic acinar cells after duct ligation", ARCH HISTOL CYTOL., (1995), vol. 58, pages 221 - 9
    - APELQVIST A; LI H; SOMMER L; BEATUS P; ANDERSON DJ; HONJO T; HRABE DE ANGELIS M; LENDAHL U; EDLUND H., "Notch signalling controls pancreatic cell differentiation", NATURE, (1999), vol. 400, pages 877 - 81
    - BARKER N; VAN ES JH; KUIPERS J; KUJALA P; VAN DEN BORN M; COZIJNSEN M; HAEGEBARTH A; KORVING J; BEGTHEL H; PETERS PJ, "Identification of stem cells in small intestine and colon by marker gene Lgr5", NATURE, (2007), vol. 449, pages 1003 - 7
    - BONNER-WEIR, S.; WEIR, G.C, "New sources of pancreatic beta-cells", NAT. BIOTECHNOL, (2005), vol. 23, pages 857 - 861
    - BONNER-WEIR, S.; TANEJA, M.; WEIR, G.C.; TATARKIEWICZ, K.; SONG, K.H.; SHARMA, A.; O'NEIL, J.J, "In vitro cultivation of human islets from expanded ductal tissue", PROC. NATL. ACAD. SCI. USA, (2000), vol. 97, pages 7999 - 8004
    - BOUWENS, L.; ROOMAN, I., "Regulation of pancreatic beta-cell mass", PHYSIOL, REV., (2005), vol. 85, pages 1255 - 1270
    - DOR, Y.; BROWN, J.; MARTINEZ, 0.1.; MELTON, D.A, "Adult pancreatic beta-cells are formed by self-duplication rather than stem-cell differentiation", NATURE, (2004), vol. 429, pages 41 - 46
    - GITHENS S; SCHEXNAYDER JA; DESAI K; PATKE CL, "Rat pancreatic interlobular duct epithelium: isolation and culture in collagen gel", IN VITRO CELL DEV BIOL., (1989), vol. 25, pages 679 - 88
    - GRADWOHL, G.; DIERICH, A; LEMEUR, M.; GUILLEMOT, F, "neurogenin3 is required for the development of the four endocrine cell lineages of the pancreas", PROC. NATL. ACAD. SCI. USA, (2000), vol. 97, pages 1607 - 1611
    - GU, G.; DUBAUSKAITE, J.; MELTON, D.A., "Direct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors", DEVELOPMENT, (2002), vol. 129, pages 2447 - 2457
    - HAO, E.; TYRBERG, B.; ITKIN-ANSARI, P.; LAKEY, J.R.; GERON, I.; MONOSOV, E.Z.; BARCOVA, M.; MERCOLA, M.; LEVINE, F, "Beta-cell differentiation from nonendocrine epithelial cells of the adult human pancreas", NAT. MED, (2006), vol. 12, pages 310 - 316
    - JAKS V; BARKER N; KASPER M; VAN ES JH; SNIPPERT HJ; CLEVERS H; TOFTGARD R, "Lgr5 marks cycling, yet long-lived, hair follicle stem cells", NAT GENET, (2008), vol. 40, pages 1291 - 9
    - LEFEBVRE VH; OTONKOSKI T; USTINOV J; HUOTARI MA; PIPELEERS DG; BOUWENS L, "Culture of adult human islet preparations with hepatocyte growth factor and 804G matrix is mitogenic for duct cells but not for beta-cells", DIABETES, (1998), vol. 47, pages 134 - 7
    - LUSTIG B; JERCHOW B; SACHS M; WEILER S; PIETSCH T; KARSTEN U; VAN DE WETERING M; CLEVERS H; SCHLAG PM; BIRCHMEIER W, "Negative feedback loop of Wnt signaling through upregulation of conductin/axin2 in colorectal and liver tumors", J. MOL CELL BIOT, (2002), pages 84 - 93
    - MIRALLES F; CZERNICHOW P; OZAKI K; ITOH N; SCHARFMANN R, "Signaling through fibroblast growth factor receptor 2b plays a key role in the development of the exocrine pancreas", PROC NATL ACAD SCI USA., (1999), vol. 96, pages 6267 - 72
    - RAMIYA, V.K.; MARAIST, M.; ARFORS, K.E.; SCHATZ, D.A.; PECK, A.B.; CORNELIUS, J.G., "Reversal of insulin-dependent diabetes using islets generated in vitro from pancreatic stem cells", NAT. MED., (2000), vol. 6, pages 278 - 282
    - ROOMAN I; HEREMANS Y; HEIMBERG H; BOUWENS L, "Modulation of rat pancreatic acinoductal transdifferentiation and expression of PDX- in vitro", DIABETOLOGIA, (200007), vol. 43, no. 7, pages 907 - 14
    - ROOMAN I; LARDON J; FLAMEZ D; SCHUIT F; BOUWENS L, "Mitogenic effect of gastrin and expression of gastrin receptors in duct-like cells of rat pancreas", GASTROENTEROLOGY, (2001), vol. 121, pages 940 - 9
    - SATO T; VRIES RG; SNIPPERT HJ; VAN DE WETERING M; BARKER N; STANGE DE; VAN ES JH; ABO A; KUJALA P; PETERS PJ, "Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche", NATURE, (2009), vol. 459, pages 262 - 5
    - SCHWITZGEBEL, V.M.; SCHEEL, D.W.; CONNERS, J.R; KALAMARAS, J.; LEE, J.E.; ANDERSON, D.J.; SUSSEL, L.; JOHNSON, J.D.; GERMAN, M.S, "Expression of neurogenin3 reveals an islet cell precursor population in the pancreas", DEVELOPMENT, (2000), vol. 127, pages 3533 - 3542
    - SEABERG, R.M.; SMUKLER, S.R.; KIEFFER, T.J.; ENIKOLOPOV, G.; ASGHAR, Z.; WHEELER, M.B.; KORBUTT, G.; VAN DER KOOY, D.; 2004, "Clonal identification of multipotent precursors from adult mouse pancreas that generate neural and pancreatic lineages", NAT. BIOTECHNOL., vol. 22, pages 1115 - 1124
    - SUZUKI, A.; NAKAUCHI, H.; TANIGUCHI, H., "Prospective isolation of multipotent pancreatic progenitors using flow-cytometric cell sorting", DIABETES, (2004), vol. 53, pages 2143 - 2152
    - TETA, M.; RANKIN, M.M.; LONG, S.Y.; STEIN, G.M.; KUSHNER, J.A, "Growth and regeneration of adult beta cells does not involve specialized progenitors", DEV. CELL, (2007), vol. 12, pages 817 - 826
    - TRAUTMANN B; SCHLITT HJ; HAHN EG; LOHR M, "Isolation, culture, and characterization of human pancreatic duct cells", PANCREAS, (1993), vol. 8, pages 248 - 54
    - WANG, R.N.; KLOPPEL, G.; BOUWENS, L, "Duct- to islet-cell differentiation and islet growth in the pancreas of duct-ligated adult rats", DIABETOLOGIA, (1995), vol. 38, pages 1405 - 1411
    - XU X; D'HOKER J; STANGE G; BONNE S; DE LEU N; XIAO X; VAN DE CASTEELE M; MELLITZER G; LING Z; PIPELEERS D, "Beta cells can be generated from endogenous progenitors in injured adult mouse pancreas", CELL, (2008), vol. 132, no. 2, pages 197 - 207
    - CHEN ET AL., NAT CHEM BIOL., (200902), vol. 5, no. 2, pages 100 - 7
    - GREGORIEFF; CLEVERS, GENES DEV, (20050415), vol. 19, no. 8, pages 877 - 90
    - SATO ET AL., NATURE, (20090514), vol. 459, no. 7244, pages 262 - 5
    - BJERKNES; CHENG, AM J PHYSIOL GASTROINTEST LIVER PHYSIOL., (200209), vol. 283, no. 3, pages G767 - 77
    - WILLERT ET AL., NATURE, (20030522), vol. 423, no. 6938, pages 448 - 52
    - VAN DE WETERING ET AL., CANCER RES, (20010101), vol. 61, no. 1, pages 278 - 84
    - BARKER, N. ET AL., "Identification of stem cells in small intestine and colon by marker gene Lgr5", NATURE, (2007), vol. 449, pages 1003 - 7
    - BJERKNES, M.; CHENG, H., "Intestinal epithelial stem cells and progenitors", METHODS ENZYMOL, (2006), vol. 419, pages 337 - 83
    - BARKER, N.; VAN DE WETERING, M.; CLEVERS, H, "The intestinal stem cell", GENES DEV, (2008), vol. 22, pages 1856 - 64
    - EVANS, G, S.; FLINT, N.; SOMERS, A. S.; EYDEN, B.; POTTEN, C. S., "The development of a method for the preparation of rat intestinal epithelial cell primary cultures", J CELL SCI, (1992), vol. 101, pages 219 - 31
    - WHITEHEAD, R. H.; DEMMLER, K.; ROCKMAN, S. P.; WATSON, N. K., "Clonogenic growth of epithelial cells from normal colonic mucosa from both mice and humans", GASTROENTEROLOGY, (1999), vol. 117, pages 858 - 65
    - FUKAMACHI, H., "Proliferation and differentiation of fetal rat intestinal epithelial cells in primary serum-free culture", J CELL SCI, (1992), vol. 103
    - PERREAULT, N.; JEAN-FRANCOIS, B, "Use of the dissociating enzyme thermolysin to generate viable human normal intestinal epithelial cell cultures", EXP CELL RES, (1996), vol. 224, pages 354 - 64
    - KORINEK, V. ET AL., "Depletion of epithelial stem-cell compartments in the small intestine of mice lacking Tcf-4", NAT GENET, (1998), vol. 19, pages 379 - 83
    - PINTO, D.; GREGORIEFF, A.; BEGTHEL, H; CLEVERS, H, "Canonical Wnt signals are essential for homeostasis of the intestinal epithelium", GENES DEV, (2003), vol. 17, pages 1709 - 13
    - KUHNERT, F. ET AL., "Essential requirement for Wnt signaling in proliferation of adult small intestine and colon revealed by adenoviral expression of Dickkopf-l", PROC NATL ACAD SCI U S A, (2004), vol. 101, pages 266 - 71
    - KIM, K. A ET AL., "Mitogenic influence of human R-spondin on the intestinal epithelium", SCIENCE, (2005), vol. 309, pages 1256 - 9
    - DIGNASS, A. U.; STURM, A, "Peptide growth factors in the intestine", EUR J GASTROENTEROL HEPATOL, (2001), vol. 13, pages 763 - 70
    - HARAMIS, A. P ET AL., "De novo crypt formation and juvenile polyposis on BMP inhibition in mouse intestine", SCIENCE, (2004), vol. 303, pages 1684 - 6
    - HOFMANN, C ET AL., "Cell-cell contacts prevent anoikis in primary human colonic epithelial cells", GASTROENTEROLOGY, (2007), vol. 132, pages 587 - 600
    - SASAKI, T.; GILTAY, R.; TALTS, U.; TIMPL, R; TALTS, J. F., "Expression and distribution of laminin alpha and alpha2 chains in embryonic and adult mouse tissues: an immunochemical approach", EXP CELL RES, (2002), vol. 275, pages 185 - 99
    - STINGL, J.; EAVES, C. J.; ZANDIEH, I.; EMERMAN, J., "T. Characterization of bipotent mammary epithelial progenitor cells in normal adult human breast tissue", BREAST CANCER RES TREAT, (2001), vol. 67, pages 93 - 109
    - ST CLAIR, W. H.; OSBORNE, J. W., "Crypt fission and crypt number in the small and large bowel of postnatal rats", CELL TISSUE KINET, (1985), vol. 18, pages 255 - 62
    - BATLLE, E ET AL., "Beta-catenin and TCF mediate cell positioning in the intestinal epithelium by controlling the expression of EphB/ephrinB", CELL, (2002), vol. 111, pages 251 - 63
    - SORIANO, P., "Generalized lacZ expression with the ROSA26 Cre reporter strain", NAT GENET, (1999), vol. 21, pages 70 - 1
    - STINGL, J. ET AL., "Purification and unique properties of mammary epithelial stem cells", NATURE, (2006), vol. 439, pages 993 - 7
    - WATANABE, K. ET AL., "A ROCK inhibitor permits survival of dissociated human embryonic stem cells", NAT BIOTECHNOL, (2007), vol. 25, pages 681 - 6
    - VAN ES, J. H ET AL., "Notch/gamma-secretase inhibition turns proliferative cells in intestinal crypts and adenomas into goblet cells", NATURE, (2005), vol. 435, pages 959 - 63
    - LI, L. ET AL., "The human homolog of rat Jagged 1 expressed by marrow stroma inhibits differentiation of 32D cells through interaction with Notch 1", IMMUNITY, (1998), vol. 8, pages 43 - 55
    - CHENG, H.; LEBLOND, C. P., "Origin, differentiation and renewal of the four main epithelial cell types in the mouse small intestine. I. Columnar cell", AM J ANAT, (1974), vol. 141, pages 461 - 79
    - POWELL, D. W. ET AL., "Myofibroblasts. II. Intestinal subepithelial myofibroblasts", AM J PHYSIOL, (1999), vol. 277, pages C 183 - 201
    - YEN, T. H.; WRIGHT, N. A., "The gastrointestinal tract stem cell niche", STEM CELL REV, (2006), vol. 2, pages 203 - 12
    - KEDINGER, M. ET AL., "Intestinal epithelial-mesenchymal cell interactions", ANN N Y ACAD SCI, (1998), vol. 859, pages 1 - 17
    - SPRADLING, A.; DRUMMOND-BARBOSA, D.; KAI, T., "Stem cells find their niche", NATURE, (2001), vol. 414, pages 98 - 104
    - LI, L.; XIE, T., "Stem cell niche: structure and function", ANNU REV CELL DEV BIOL, (2005), vol. 21, pages 605 - 31
    - BINNERTS ME ET AL., PNAS, (2007), vol. 104, pages 14700 - 5
    - SAWADA ET AL., INT J EXP PATHOL, (1991), vol. 72, pages 407 - 21
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