Deutsch English Français

Contact

EP3365441

European procedure

Quick help

Maintenance news

Scheduled maintenance

Regular maintenance outages:
between 05.00 and 05.15 hrs CET (Monday to Sunday).

Other outages
Availability
Register Forum

2022.02.11

More...

News flashes

News flashes

New version of the European Patent Register - SPC information for Unitary Patents.

2024-03-06

More...

Extract from the Register of European Patents

EP About this file: EP3365441

EP3365441 - TYPE VI-B CRISPR ENZYMES AND SYSTEMS [Right-click to bookmark this link]
Status	Examination is in progress Status updated on 29.04.2019 Database last updated on 12.07.2024
Former	Request for examination was made Status updated on 27.07.2018
Former	The international publication has been made Status updated on 28.04.2017
Former	unknown Status updated on 18.11.2016
Most recent event Tooltip	28.03.2024	New entry: Reply to examination report
Applicant(s)	For all designated states The Broad Institute, Inc. 415 Main Street Cambridge, MA 02142 / US
	For all designated states Massachusetts Institute of Technology 77 Massachusetts Avenue Cambridge, MA 02142 / US
	For all designated states Zhang, Feng 100 Pacific Street, Apt. 11 Cambridge, MA 02139 / US
	For all designated states Smargon, Aaron 70 Pacific Street 830 Cambridge, MA 02139 / US
	For all designated states Pyzocha, Neena 937-2nd NH Turnpike New Boston, NH 03070 / US
	For all designated states Cox, David Benjamin Turitz 950 Massachusetts Avenue Apt. 217 Cambridge, MA 02139 / US
	For all designated states Lander, Eric, S. 74r Fayerweather Street Cambridge, MA 02138 / US
	[2019/03]
Former [2018/35]	For all designated states The Broad Institute Inc. 415 Main Street Cambridge, MA 02142 / US
	For all designated states Massachusetts Institute of Technology 77 Massachusetts Avenue Cambridge, MA 02142 / US
	For all designated states Zhang, Feng 100 Pacific Street, Apt. 11 Cambridge, MA 02139 / US
	For all designated states Smargon, Aaron 70 Pacific Street 830 Cambridge, MA 02139 / US
	For all designated states Pyzocha, Neena 937-2nd NH Turnpike New Boston, NH 03070 / US
	For all designated states Cox, David Benjamin Turitz 950 Massachusetts Avenue Apt. 217 Cambridge, MA 02139 / US
	For all designated states Lander, Eric, S. 74r Fayerweather Street Cambridge, MA 02138 / US
Inventor(s)	01 / ZHANG, Feng 74R Fayerweather Street Cambridge, MA 02138 / US
	02 / SMARGON, Aaron 74R Fayerweather Street Cambridge, MA 02138 / US
	03 / PYZOCHA, Neena 74R Fayerweather Street Cambridge, MA 02138 / US
	04 / COX, David Benjamin Turitz 74R Fayerweather Street Cambridge, MA 02138 / US
	05 / LANDER, Eric S. 74R Fayerweather Street Cambridge, MA 02138 / US
	[2018/35]
Representative(s)	De Clercq & Partners Edgard Gevaertdreef 10a 9830 Sint-Martens-Latem / BE
	[2018/35]
Application number, filing date	16791741.8	21.10.2016
	[2018/35]
	WO2016US58302
Priority number, date	US201562245270P	22.10.2015 Original published format: US 201562245270 P
	US201662296548P	17.02.2016 Original published format: US 201662296548 P
	US201662376382P	17.08.2016 Original published format: US 201662376382 P
	US201662376367P	17.08.2016 Original published format: US 201662376367 P
	[2018/35]
Filing language	EN
Procedural language	EN
Publication	Type:	A1 Application with search report
	No.:	WO2017070605
	Date:	27.04.2017
	Language:	EN
	[2017/17]
	Type:	A1 Application with search report
	No.:	EP3365441
	Date:	29.08.2018
	Language:	EN
	The application published by WIPO in one of the EPO official languages on 27.04.2017 takes the place of the publication of the European patent application.
	[2018/35]
Search report(s)	International search report - published on:	EP	27.04.2017
Classification	IPC:	C12N9/22, C12N15/55, C12N15/113	[2018/35]
	CPC:	C12N15/113 (EP,CN,KR,US); C12N9/22 (EP,CN,KR,US); C12N15/102 (EP,KR,US); C12N15/111 (EP,KR,US); C12N15/63 (EP,CN,KR,US); C12N15/74 (KR,US); (+) C12Q1/6876 (CN,KR,US); G16B25/00 (EP,US); G16B25/10 (KR); G16B30/00 (EP,US); G16B30/10 (EP,KR,US); G16B30/20 (EP,US); C12N2310/20 (EP,KR,US); C12Q2600/156 (KR,US) (-)
Designated contracting states	AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LI, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR [2018/35]
Title	German:	CRISPR-ENZYME DES TYPS VI-B UND SYSTEME	[2018/35]
	English:	TYPE VI-B CRISPR ENZYMES AND SYSTEMS	[2018/35]
	French:	ENZYMES ET SYSTÈMES CRISPR DE TYPE VI-B	[2018/35]
Entry into regional phase	22.05.2018	National basic fee paid
	22.05.2018	Designation fee(s) paid
	22.05.2018	Examination fee paid
Examination procedure	22.05.2018	Examination requested [2018/35]
	22.05.2018	Date on which the examining division has become responsible
	10.12.2018	Amendment by applicant (claims and/or description)
	03.05.2019	Despatch of a communication from the examining division (Time limit: M04)
	22.08.2019	Reply to a communication from the examining division
	05.02.2020	Despatch of a communication from the examining division (Time limit: M04)
	15.06.2020	Reply to a communication from the examining division
	23.10.2020	Despatch of a communication from the examining division (Time limit: M04)
	26.10.2020	Despatch of a communication from the examining division (Time limit: M04)
	05.03.2021	Reply to a communication from the examining division
	30.01.2023	Despatch of a communication from the examining division (Time limit: M04)
	04.05.2023	Reply to a communication from the examining division
	27.11.2023	Despatch of a communication from the examining division (Time limit: M04)
	27.03.2024	Reply to a communication from the examining division
Fees paid	Renewal fee
	29.10.2018	Renewal fee patent year 03
	28.10.2019	Renewal fee patent year 04
	27.10.2020	Renewal fee patent year 05
	27.10.2021	Renewal fee patent year 06
	27.10.2022	Renewal fee patent year 07
	27.10.2023	Renewal fee patent year 08
Opt-out from the exclusive Tooltip competence of the Unified Patent Court	See the Register of the Unified Patent Court for opt-out data
	Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Cited in	International search	[E]WO2016205749 (BROAD INST INC [US], et al) [E] 37-46* claims 50-60 *;
		[XD] - "Bergeyella zoohelcum ATCC 43767 hypothetical protein", ENA Sequence, (20120919), Database accession no. EKB54193, URL: EBI, XP002765998 [XD] 47-49 * sequence . *
		[XA] - ARYN A. PRICE ET AL, "Cas9-mediated targeting of viral RNA in eukaryotic cells", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, US, (20150512), vol. 112, no. 19, doi:10.1073/pnas.1422340112, ISSN 0027-8424, pages 6164 - 6169, XP055335247 [X] 37-46 * the whole document * [A] 1-36,50,51 DOI: http://dx.doi.org/10.1073/pnas.1422340112
		[A] - KIRA S. MAKAROVA ET AL, "An updated evolutionary classification of CRISPR-Cas systems", NATURE REVIEWS. MICROBIOLOGY, GB, (20150928), vol. 13, no. 11, doi:10.1038/nrmicro3569, ISSN 1740-1526, pages 722 - 736, XP055271841 [A] 1-36,50,51 * the whole document * DOI: http://dx.doi.org/10.1038/nrmicro3569
		[XPI] - Aaron Andrew Smargon, "An Expanded Search for RNA-Programmable Genomic Engineering Effectors", (20160601), pages 1 - 33, URL: https://www.google.nl/url?sa=t&rct=j&q=&esrc=s&source=web&cd=4&ved=0ahUKEwi4qcD4pcbRAhWL2BoKHWj_BjoQFggrMAM&url=https://dspace.mit.edu/bitstream/handle/1721.1/105959/965549261-MIT.pdf?sequence=1&usg=AFQjCNEpTUvmbxqx4NFXht4RK1DS1TEzIw&bvm=bv.144224172,d.d2s, (20170116), XP055335503 [XP] 47-49 * the whole document * [I] 1-36,50,51
	by applicant	US4186183
		US4217344
		US4235871
		US4261975
		US4485054
		US4501728
		EP0264166
		US4774085
		US4797368
		US4837028
		US4873316
		US4897355
		US4946787
		US5049386
		WO9116024
		WO9117424
		US5173414
		WO9301294
		US5210015
		WO9324641
		US5445934
		US5543158
		US5563055
		US5580859
		WO9639154
		US5589466
		US5593972
		WO9703211
		US5789156
		US5814618
		US5846946
		US5855913
		US5985309
		US6007845
		US2003087817
		US6603061
		US2004013648
		US6750059
		US2004142476
		US2004171156
		US2005019923
		EP1519714
		EP1664316
		US2006281180
		US2007025970
		US2007054961
		EP1766035
		EP1781593
		US7259015
		US7303910
		US7351585
		WO2008042156
		WO2008064289
		US2008267903
		US2009007284
		US2009017543
		US2009100536
		US2009111106
		WO2010061186
		US7745651
		US7776321
		US7799565
		US7803397
		US7838658
	US2010317109
	US7868149
	US7901708
	WO2011028929
	US2011059502
	US7915399
	US2011117189
	US7982027
	US2011195123
	US8044019
	US2011265198
	US8058069
	US2011293571
	US2011293703
	US2012017290
	US8101741
	US8188263
	US8236943
	WO2012135025
	US2012251560
	US2012251618
	US8283333
	US2012295960
	US8372951
	US8404658
	US8454972
	US2013185823
	US2013236946
	US2013245107
	US2013244279
	US2013252281
	US8575305
	US2013302401
	US8614194
	WO2014018423
	US8697359
	US8709843
	WO2014093622
	WO2014093635
	WO2014093701
	US2014170753
	WO2014093661
	WO2014093694
	WO2014093595
	WO2014093718
	WO2014093709
	WO2014093655
	WO2014093712
	US2014179770
	US2014179006
	US2014186919
	US2014189896
	US2014186958
	US2014186843
	US8771945
	US8795965
	EP2764103
	US2014227787
	US2014234972
	US2014242700
	US2014242699
	US2014242664
	EP2771468
	US2014248702
	US2014256046
	US2014273234
	US2014273232
	US2014273231
	US2014287938
	EP2784162
	US2014310830
	US8865406
	US8871445
	US2014335620
	US8889418
	US8889356
	US8895308
	US2014357530
	US8906616
	WO2014204723
	WO2014204724
	WO2014204725
	WO2014204726
	WO2014204727
	WO2014204728
	WO2014204729
	US8932814
	US8945839
	EP2771468
	US8993233
	US8999641
	EP2784162
	WO2015089419
	WO2015089351
	- SAMAI ET AL., "Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas Immunity", CELL, (20150521), vol. 161, pages 1164 - 1174
	- NAKAMURA, Y. ET AL., "Codon usage tabulated from the international DNA sequence databases: status for the year 2000", NUCL. ACIDS RES., (2000), vol. 28, page 292
	- PLATT, CELL, (2014), vol. 159, no. 2, pages 440 - 455
	- MAKAROVA KS; KOONIN EV, METHODS MOL BIOL., (2015), vol. 1311, pages 47 - 75
	- EDGAR, R.C., "PILER-CR: fast and accurate identification of CRISPR repeats", BMC BIOINFORMATICS, (20070120), vol. 8, page 18
	- SODING ET AL., NUCLEIC ACIDS RESEARCH, (2005), vol. 33, pages W244 - 8
	- HALE ET AL., GENES DEV, (2014), vol. 28, pages 2432 - 2443
	- HALE ET AL., CELL, (2009), vol. 139, pages 945 - 956
	- PENG ET AL., NUCLEIC ACIDS RESEARCH, (2015), vol. 43, pages 406 - 417
	- BURNETT; ROSSI, CHEM BIOL., (2012), vol. 19, no. 1, pages 60 - 71
	- DEL GATTO-KONCZAK ET AL., MOL CELL BIOL., (199901), vol. 19, no. L, pages 251 - 60
	- GRAVELY BR; MANIATIS T, MOL CELL, (1998), pages 765 - 71
	- HIGUCHI M ET AL., CELL, (1993), vol. 75, pages 1361 - 70
	- MIYAMURA Y ET AL., AM J HUM GENET., (2003), vol. 73, pages 693 - 9
	- FU ET AL., NAT REV GENET., (2014), vol. 15, no. 5, pages 293 - 306
	- MINVIELLE-SEBASTIA L ET AL., CURR OPIN CELL BIOL., (1999), vol. 11, pages 352 - 7
	- BRAIS B ET AL., NAT GENET., (1998), vol. 18, pages 164 - 7
	- KATAHIRA J ET AL., EMBO J., (1999), vol. 18, pages 2593 - 609
	- DE GREGORIO ET AL., EMBO J., (1999), vol. 18, no. 17, pages 4865 - 74
	- HUTTELMAIER S ET AL., NATURE, (2005), vol. 438, pages 512 - 5
	- KEENE JD, PROC NATL ACAD SCI USA., (1999), vol. 96, pages 5 - 7
	- PENG SS ET AL., EMBO J., (1988), vol. 17, pages 3461 - 70
	- HINMAN MN; LOU H, CELL MOL LIFE SCI, (2008), vol. 65, pages 3168 - 81
	- SZABO A ET AL., CELL, (1991), vol. 67, pages 325 - 33
	- CHEN ET AL., SCIENCE, (2015), vol. 348, no. 6233
	- GEARY ET AL., SCIENCE, (2014), vol. 345, no. 6198, pages 799 - 804
	- TOPILINA; MILLS, MOB DNA., (20140204), vol. 5, no. 1, page 5
	- KONERMANN S ET AL., NATURE, (2013), vol. 500, pages 472 - 476
	- ZETSCHE ET AL., NAT BIOTECHNOL., (2015), vol. 33, no. 2, pages 139 - 42
	- GOLDFLESS ET AL., NUCLEIC ACIDS RES., (2012), vol. 40, no. 9, page E64
	- WROBLESKA ET AL., NAT BIOTECHNOL., (201508), vol. 33, no. 8, pages 839 - 841
	- MACKAY JP ET AL., NAT STRUCT MOL BIOL., (201103), vol. 18, no. 3, pages 256 - 61
	- NELLES ET AL., BIOESSAYS, (201507), vol. 37, no. 7, pages 732 - 9
	- ABIL Z; ZHAO H, MOL BIOSYST., (201510), vol. LL, no. 10, pages 2658 - 65
	- MIYAZAKI, J AM CHEM SOC., (20120307), vol. 134, no. 9, pages 3942 - 3945
	- NATURE METHODS, (2008), vol. 5
	- BANASZYNSKI LA; CHEN LC; MAYNARD-SMITH LA; OOI AG; WANDLESS TJ, "A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules", CELL, (2006), vol. 126, pages 995 - 1004
	- BANASZYNSKI LA; SELLMYER MA; CONTAG CH; WANDLESS TJ; THORNE SH, "Chemical control of protein stability and function in living mice", NAT MED., (2008), vol. 14, pages 1123 - 1127
	- MAYNARD-SMITH LA; CHEN LC; BANASZYNSKI LA; OOI AG; WANDLESS TJ, "A directed approach for engineering conditional protein stability using biologically silent small molecules", THE JOURNAL OF BIOLOGICAL CHEMISTRY, (2007), vol. 282, pages 24866 - 24872
	- RODRIGUEZ, CHEM BIOL., (20120323), vol. 19, no. 3, pages 391 - 398
	- BUCKHOLZ, R.G.; GLEESON, M.A., BIOTECHNOLOGY (NY, (1991), vol. 9, no. 11, pages 1067 - 72
	- FU ET AL., TRANSGENIC RES., (200002), vol. 9, no. 1, pages 11 - 9
	- KAWAMATA ET AL., PLANT CELL PHYSIOL, (1997), vol. 38, pages 792 - 803
	- YAMAMOTO ET AL., PLANT J, (1997), vol. 12, pages 255 - 65
	- HIRE, PLANT MOL BIOL, (1992), vol. 20, pages 207 - 18
	- KUSTER ET AL., PLANT MOL BIO, (1995), vol. 29, pages 759 - 72
	- CAPANA ET AL., PLANT MOL BIOL, (1994), vol. 25, pages 681 - 91
	- DE VEYLDER ET AL., PLANT CELL PHYSIOL, (1997), vol. 38, pages 568 - 77
	- ONO ET AL., BIOSCI BIOTECHNOL BIOCHEM, (2004), vol. 68, pages 803 - 7
	- GATZ, MOL GEN GENET, (1991), vol. 227, pages 229 - 37
	- "Protein Transport into Chloroplasts", ANNUAL REVIEW OF PLANT BIOLOGY, (2010), vol. 61, pages 157 - 180
	- KAWAI ET AL., BIOENG BUGS., (201011), vol. 1, no. 6, pages 395 - 403
	- SCHOLTHOF ET AL., ANNU REV PHYTOPATHOL., (1996), vol. 34, pages 299 - 323
	- SAINSBURY F. ET AL., PLANT BIOTECHNOL J., (200909), vol. 7, no. 7, pages 682 - 93
	- DAVEY ET AL., PLANT MOL BIOL., (198909), vol. 13, no. 3, pages 273 - 85
	- GALLIE, PLANT CELL REPORTS, (1993), vol. 13, pages 119 - 122
	- RAMAKRISHNA, GENOME RES., (201406), vol. 24, no. 6, pages 1020 - 7
	- PLANT MOL. BIOL., (1993), vol. 21, no. 6, page 1163,1170
	- KIM ET AL., PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY (IMPACT FACTOR: 2.01
	- SHARMA ET AL., ACADEMIC JOURNALS, (2015), vol. 12, no. 18, pages 2303 - 2312
	- GREEN J.M, PEST MANAGEMENT SCIENCE, vol. 70, no. 9, pages 1351 - 1357
	- BOCOBZA; AHARONI, PLANT J., (20140617), vol. 79, no. 4, pages 693 - 703
	- CHEAH MT ET AL., NATURE, (2007), vol. 447, no. 7143, pages 497 - 500
	- YOUNIS ET AL., INT. J. BIOL. SCI., (2014), vol. 10, page 1150
	- KARLIN; ALTSCHUL, PROC. NATL. ACAD. SCI. USA, (1990), vol. 87, pages 2264 - 68
	- KARLIN; ALTSCHUL, PROC. NATL. ACAD. SCI. USA, (1993), vol. 90, pages 5873 - 77
	- ALTSCHUL ET AL., J. MOL. BIOL., (1990), vol. 215, pages 403 - 10
	- ALTSCHUL ET AL., NUCLEIC ACIDS RES., (1997), vol. 25, no. 17, pages 3389 - 3402
	- GOEDDEL, GENE EXPRESSION TECHNOLOGY: METHODS IN ENZYMOLOGY, ACADEMIC PRESS, (1990), vol. 185
	- BOSHART ET AL., CELL, (1985), vol. 41, pages 521 - 530
	- MOL. CELL. BIOL., (1988), vol. 8, no. 1, pages 466 - 472
	- PROC. NATL. ACAD. SCI. USA., (1981), vol. 78, no. 3, pages 1527 - 31
	- NUCLEIC ACIDS RES., (1981), vol. 9, pages 133 - 148
	- A.R. GRUBER ET AL., CELL, (2008), vol. 106, no. 1, pages 23 - 24
	- PA CARR; GM CHURCH, NATURE BIOTECHNOLOGY, (2009), vol. 27, no. 12, pages 1151 - 62
	- KONERMANN ET AL., GENOME-SCALE TRANSCRIPTION ACTIVATION BY AN ENGINEERED CRISPR-CAS9 COMPLEX
	- SHEN ET AL., FEBS LET., (2003), vol. 539, pages 111 - 114
	- XIA ET AL., NAT. BIOTECH., (2002), vol. 20, pages 1006 - 1010
	- REICH ET AL., MOL. VISION., (2003), vol. 9, pages 210 - 216
	- SORENSEN ET AL., J. MOL. BIOL., (2003), vol. 327, pages 761 - 766
	- LEWIS ET AL., NAT. GEN., (2002), vol. 32, pages 107 - 108
	- SIMEONI ET AL., NAR, (2003), vol. 31, no. 11, pages 2717 - 2724
	- TOLENTINO ET AL., RETINA, vol. 24, no. 4, page 660
	- CHO, S.; GOLDBERG, M.; SON, S.; XU, Q.; YANG, F.; MEI, Y.; BOGATYREV, S.; LANGER, R.; ANDERSON, D., "Lipid-like nanoparticles for small interfering RNA delivery to endothelial cells", ADVANCED FUNCTIONAL MATERIALS, (2010), vol. 19, pages 3112 - 3118
	- SCHROEDER, A.; LEVINS, C.; CORTEZ, C.; LANGER, R.; ANDERSON, D., "Lipid-based nanotherapeutics for siRNA delivery", JOURNAL OF INTERNAL MEDICINE, (2010), vol. 267, pages 9 - 21
	- EL-ANDALOUSSI S ET AL., "Exosome-mediated delivery of siRNA in vitro and in vivo", NAT PROTOC., (20121115), vol. 7, no. 12, pages 2112 - 26
	- HUMAN GENE THERAPY, (201106), vol. 22, pages 711 - 719
	- ZOU ET AL., HUMAN GENE THERAPY, (201104), vol. 22, pages 465 - 475
	- GRIMM, D. ET AL., J. VIROL., (2008), vol. 82, pages 5887 - 5911
	- BALAGAAN, J GENE MED, (2006), vol. 8, pages 275 - 285
	- BINLEY ET AL., HUMAN GENE THERAPY, (201209), vol. 23, pages 980 - 991
	- DIGIUSTO ET AL., SCI TRANSL MED, (2010), vol. 2, page 36RA43
	- SU X; FRICKE J; KAVANAGH DG; IRVINE DJ, "In vitro and in vivo mRNA delivery using lipid-enveloped pH-responsive polymer nanoparticles", MOL PHARM., (20110401), vol. 8, no. 3, pages 774 - 87
	- MAZZA, M. ET AL., ACSNANO, (2013), vol. 7, no. 2, pages 1016 - 1026
	- SIEW, A. ET AL., MOL PHARM, (2012), vol. 9, no. 1, pages 14 - 28
	- LALATSA, A. ET AL., J CONTR REL, (2012), vol. 161, no. 2, pages 523 - 36
	- LALATSA, A. ET AL., MOL PHARM, (2012), vol. 9, no. 6, pages 1665 - 80
	- LALATSA, A. ET AL., MOL PHARM, (2012), vol. 9, no. 6, pages 1764 - 74
	- GARRETT, N.L. ET AL., J BIOPHOTONICS, (2012), vol. 5, no. 5-6, pages 458 - 68
	- GARRETT, N.L. ET AL., J RAMAN SPECT, (2012), vol. 43, no. 5, pages 681 - 688
	- AHMAD, S. ET AL., J ROYAL SOC INTERFACE, (2010), vol. 7, pages 423 - 33
	- UCHEGBU, I.F., EXPERT OPIN DRUG DELIV, (2006), vol. 3, no. 5, pages 629 - 40
	- QU, X., BIOMACROMOLECULES, (2006), vol. 7, no. 12, pages 3452 - 9
	- UCHEGBU, I.F. ET AL., INT J PHARM, (2001), vol. 224, pages 185 - 199
	- ALABI ET AL., PROC NATL ACAD SCI USA., (20130806), vol. 110, no. 32, pages 12881 - 6
	- ZHANG ET AL., ADV MATER., (20130906), vol. 25, no. 33, pages 4641 - 5
	- JIANG ET AL., NANO LETT., (20130313), vol. 13, no. 3, pages 1059 - 64
	- KARAGIANNIS ET AL., ACS NANO, (20121023), vol. 6, no. 10, pages 8484 - 7
	- WHITEHEAD ET AL., ACS NANO., (20120828), vol. 6, no. 8, pages 6922 - 9
	- LEE ET AL., NAT NANOTECHNOL., (20120603), vol. 7, no. 6, pages 389 - 93
	- COELHO ET AL., N ENGL J MED, (2013), vol. 369, pages 819 - 29
	- TABERNERO ET AL., CANCER DISCOVERY, (201304), vol. 3, no. 4, pages 363 - 470
	- ROSIN ET AL., MOLECULAR THERAPY, (201112), vol. 19, no. 12, pages 1286 - 2200
	- CUTLER ET AL., J. AM. CHEM. SOC., (2011), vol. 133, pages 9254 - 9257
	- HAO ET AL., SMALL, (2011), vol. 7, pages 3158 - 3162
	- ZHANG ET AL., ACS NANO., (2011), vol. 5, pages 6962 - 6970
	- CUTLER ET AL., J. AM. CHEM. SOC., (2012), vol. 134, pages 1376 - 1391
	- YOUNG ET AL., NANO LETT., (2012), vol. 12, pages 3867 - 71
	- ZHENG ET AL., PROC. NATL. ACAD. SCI. USA., (2012), vol. 109, pages 11975 - 80
	- MIRKIN, NANOMEDICINE, (2012), vol. 7, pages 635 - 638
	- ZHANG ET AL., J. AM. CHEM. SOC., (2012), vol. 134, pages 16488 - 1691
	- WEINTRAUB, NATURE, (2013), vol. 495, pages S14 - S16
	- CHOI ET AL., PROC. NATL. ACAD. SCI. USA., (2013), vol. 110, no. 19, pages 7625 - 7630
	- JENSEN ET AL., SCI. TRANSL. MED., (2013), vol. 5, page 209RA152
	- MIRKIN ET AL., SMALL, vol. 10, pages 186 - 192
	- SCHIFFELERS ET AL., NUCLEIC ACIDS RESEARCH, (2004), vol. 32, no. 19
	- BARTLETT ET AL., PNAS, (20070925), vol. 104, no. 39
	- DAVIS ET AL., NATURE, (20100415), vol. 464
	- ALVAREZ-ERVITI ET AL., NAT BIOTECHNOL, (2011), vol. 29, page 341
	- EL-ANDALOUSSI ET AL., NATURE PROTOCOLS, (2012), vol. 7, pages 2112 - 2126
	- WAHLGREN ET AL., NUCLEIC ACIDS RESEARCH, (2012), vol. 40, no. 17, page E130
	- SPUCH; NAVARRO, JOURNAL OF DRUG DELIVERY, (2011), vol. 2011, page 12
	- MORRISSEY ET AL., NATURE BIOTECHNOLOGY, (200508), vol. 23, no. 8
	- ZIMMERMAN ET AL., NATURE LETTERS, (20060504), vol. 441
	- LI, GENE THERAPY, (2012), vol. 19, pages 775 - 780
	- GEISBERT ET AL., LANCET, (2010), vol. 375, pages 1896 - 905
	- JUDGE, J. CLIN. INVEST, (2009), vol. 119, pages 661 - 673
	- ADVANCED DRUG DELIVERY REVIEWS, (2012), vol. 64, pages 1730 - 1737
	- SEMPLE ET AL., NATURE NIOTECHNOLOGY, (201002), vol. 28, no. 2, pages 172 - 177
	- JAYARAMAN, ANGEW. CHEM. INT. ED., (2012), vol. 51, pages 8529 - 8533
	- MICHAEL S D KORMANN ET AL., "Expression of therapeutic proteins after delivery of chemically modified mRNA in mice", NATURE BIOTECHNOLOGY, (2011), vol. 29, pages 154 - 157
	- NOVOBRANTSEVA, MOLECULAR THERAPY-NUCLEIC ACIDS, (2012), vol. 1, page E4
	- MAZZA ET AL., ACS NANO., (20130226), vol. 7, no. 2, pages 1016 - 26
	- UCHEGBU; SIEW, J PHARM SCI., vol. 102, no. 2, pages 305 - 10
	- LALATSA ET AL., J CONTROL RELEASE, (20120720), vol. 161, no. 2, pages 523 - 36
	- LAWRENCE ET AL., JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, (2007), vol. 129, pages 10110 - 10112
	- AKINC ET AL., NAT. BIOTECH., (2010), vol. 26, pages 561 - 569
	- MCNAUGHTON ET AL., PROC. NATL. ACAD. SCI. USA, (2009), vol. 106, pages 6111 - 6116
	- CRONICAN ET AL., ACS CHEMICAL BIOLOGY, (2010), vol. 5, pages 747 - 752
	- CRONICAN ET AL., CHEMISTRY & BIOLOGY, (2011), vol. 18, pages 833 - 838
	- THOMPSON ET AL., METHODS IN ENZYMOLOGY, (2012), vol. 503, pages 293 - 319
	- THOMPSON, D.B. ET AL., CHEMISTRY & BIOLOGY, (2012), vol. 19, no. 7, pages 831 - 843
	- DEVEREUX ET AL., NUCLEIC ACIDS RESEARCH, (1984), vol. 12, page 387
	- ATSCHUL ET AL., J. MOL. BIOL., (1990), pages 403 - 410
	- DEVEREUX ET AL., NUC. ACIDS RESEARCH, (1984), vol. 12, page 387
	- ALTSCHUL ET AL., J. MOL. BIOL., (1990), pages 403 - 410
	- AUSUBEL ET AL., SHORT PROTOCOLS IN MOLECULAR BIOLOGY, (1999), pages 7 - 58,7-60
	- FEMS MICROBIOL LETT., (1999), vol. 174, no. 2, pages 247 - 50
	- FEMS MICROBIOL LETT., (1999), vol. 177, no. 1, pages 187 - 8
	- HIGGINS DG; SHARP PM, GENE, (1988), vol. 73, no. 1, pages 237 - 244
	- LIVINGSTONE C.D.; BARTON G.J., "Protein sequence alignments: a strategy for the hierarchical analysis of residue conservation", COMPUT. APPL. BIOSCI., (1993), vol. 9, pages 745 - 756
	- TAYLOR W.R., "The classification of amino acid conservation", J. THEOR. BIOL, (1986), vol. 119, pages 205 - 218
	- SIMON RJ ET AL., PNAS, (1992), vol. 89, no. 20, pages 9367 - 9371
	- HORWELL DC, TRENDS BIOTECHNOL., (1995), vol. 13, no. 4, pages 132 - 134
	- ZHANG ET AL., NATURE, (2012), vol. 490, no. 7421, pages 556 - 60
	- CHEN ET AL., PLOS COMPUT BIOL, (2015), vol. 11, no. 5, page EL004248
	- DEY ET AL., PROT SCI, (2013), vol. 22, pages 359 - 66
	- SMITH; JOHNSON, GENE, (1988), vol. 67, pages 31 - 40
	- AMRANN ET AL., GENE, (1988), vol. 69, pages 301 - 315
	- STUDIER ET AL., GENE EXPRESSION TECHNOLOGY: METHODS IN ENZYMOLOGY, ACADEMIC PRESS, (1990), vol. 185, pages 60 - 89
	- BALDARI ET AL., EMBO J., (1987), vol. 6, pages 229 - 234
	- KUIJAN; HERSKOWITZ, CELL, (1982), vol. 30, pages 933 - 943
	- SCHULTZ ET AL., GENE, (1987), vol. 54, pages 113 - 123
	- SMITH ET AL., MOL. CELL. BIOL., (1983), vol. 3, pages 2156 - 2165
	- LUCKLOW; SUMMERS, VIROLOGY, (1989), vol. 170, pages 31 - 39
	- SEED, NATURE, (1987), vol. 329, page 840
	- KAUFMAN ET AL., EMBO J., (1987), vol. 6, pages 187 - 195
	- PINKERT ET AL., GENES DEV., (1987), vol. 1, pages 268 - 277
	- CALAME; EATON, ADV. IMMUNOL., (1988), vol. 43, pages 235 - 275
	- WINOTO; BALTIMORE, EMBO J., (1989), vol. 8, pages 729 - 733
	- BANEIJI ET AL., CELL, (1983), vol. 33, pages 729 - 740
	- QUEEN; BALTIMORE, CELL, (1983), vol. 33, pages 741 - 748
	- BYRNE; RUDDLE, PROC. NATL. ACAD. SCI. USA, (1989), vol. 86, pages 5473 - 5477
	- EDLUND ET AL., SCIENCE, (1985), vol. 230, pages 912 - 916
	- KESSEL; GRUSS, SCIENCE, (1990), vol. 249, pages 374 - 379
	- CAMPES; TILGHMAN, GENES DEV., (1989), vol. 3, pages 537 - 546
	- ISHINO ET AL., J. BACTERIOL, (1987), vol. 169, pages 5429 - 5433
	- NAKATA ET AL., J. BACTERIOL., (1989), vol. 171, pages 3553 - 3556
	- GROENEN ET AL., MOL. MICROBIOL., (1993), vol. 10, pages 1057 - 1065
	- HOE ET AL., EMERG. INFECT. DIS., (1999), vol. 5, pages 254 - 263
	- MASEPOHL ET AL., BIOCHIM. BIOPHYS. ACTA, (1996), vol. 1307, pages 26 - 30
	- MOJICA ET AL., MOL. MICROBIOL., (1995), vol. 17, pages 85 - 93
	- JANSSEN ET AL., OMICS J. INTEG. BIOL., (2002), vol. 6, pages 23 - 33
	- MOJICA ET AL., MOL. MICROBIOL, (2000), vol. 36, pages 244 - 246
	- VAN EMBDEN ET AL., J. BACTERIOL., (2000), vol. 182, pages 2393 - 2401
	- JANSEN ET AL., MOL. MICROBIOL., (2002), vol. 43, pages 1565 - 1575
	- ZUKER; STIEGLER, NUCLEIC ACIDS RES., (1981), vol. 9, pages 133 - 148
	- A.R. GRUBER, CELL, (2008), vol. 106, no. 1, pages 23 - 24
	- ANDERSON, SCIENCE, (1992), vol. 256, pages 808 - 813
	- NABEL; FEIGNER, TIBTECH, (1993), vol. 11, pages 211 - 217
	- MITANI; CASKEY, TIBTECH, (1993), vol. 11, pages 162 - 166
	- DILLON, TIBTECH, (1993), vol. 11, pages 167 - 175
	- MILLER, NATURE, (1992), vol. 357, pages 455 - 460
	- VAN BRUNT, BIOTECHNOLOGY, (1988), vol. 6, no. 10, pages 1149 - 1154
	- VIGNE, RESTORATIVE NEUROLOGY AND NEUROSCIENCE, (1995), vol. 8, pages 35 - 36
	- KREMER; PERRICAUDET, BRITISH MEDICAL BULLETIN, (1995), vol. 51, no. 1, pages 31 - 44
	- YU ET AL., GENE THERAPY, (1994), vol. 1, pages 13 - 26
	- CRYSTAL, SCIENCE, (1995), vol. 270, pages 404 - 410
	- BLAESE ET AL., CANCER GENE THER, (1995), vol. 2, pages 291 - 297
	- BEHR ET AL., BIOCONJUGATE CHEM., (1994), vol. 5, pages 382 - 389
	- REMY ET AL., BIOCONJUGATE CHEM., (1994), vol. 5, pages 647 - 654
	- GAO ET AL., GENE THERAPY, (1995), vol. 2, pages 710 - 722
	- AHMAD ET AL., CANCER RES., (1992), vol. 52, pages 4817 - 4820
	- BUCHSCHER ET AL., J. VIROL., (1992), vol. 66, pages 2731 - 2739
	- JOHANN ET AL., J. VIROL., (1992), vol. 66, pages 1635 - 1640
	- SOMMNERFELT ET AL., VIROL, (1990), vol. 176, pages 58 - 59
	- WILSON ET AL., J. VIROL., (1989), vol. 63, pages 2374 - 2378
	- MILLER ET AL., J. VIROL., (1991), vol. 65, pages 2220 - 2224
	- WEST ET AL., VIROLOGY, (1987), vol. 160, pages 38 - 47
	- KOTIN, HUMAN GENE THERAPY, (1994), vol. 5, pages 793 - 801
	- MUZYCZKA, J. CLIN. INVEST., (1994), vol. 94, page 1351
	- TRATSCHIN ET AL., MOL. CELL. BIOL., (1985), vol. 5, pages 3251 - 3260
	- TRATSCHIN ET AL., MOL. CELL. BIOL., (1984), vol. 4, pages 2072 - 2081
	- HERMONAT; MUZYCZKA, PNAS, (1984), vol. 81, pages 6466 - 6470
	- SAMULSKI ET AL., J. VIROL., (1989), vol. 63, pages 03822 - 3828
	- CHAN-HUI ET AL., CLINICAL IMMUNOLOGY, (2003), vol. 111, pages 162 - 174
	- SHALEM, O.; SANJANA, NE.; HARTENIAN, E.; SHI, X.; SCOTT, DA.; MIKKELSON, T.; HECKL, D.; EBERT, BL.; ROOT, DE.; DOENCH, JG., SCIENCE, (20131212), vol. 343, no. 6166, pages 84 - 87
	- KONNERMAN ET AL., NATURE, (20150129), vol. 517, pages 583 - 588
	- SHENGDAR Q. TSAI; NICOLAS WYVEKENS; CYD KHAYTER; JENNIFER A. FODEN; VISHAL THAPAR; DEEPAK REYON; MATHEW J. GOODWIN; MARTIN J. ARYE, "Dimeric CRISPR RNA-guided Fokl nucleases for highly specific genome editing", NATURE BIOTECHNOLOGY, (2014), vol. 32, no. 6, pages 569 - 77
	- NEKRASOV, "Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system", PLANT METHODS, (2013), vol. 9, page 39
	- BROOKS, "Efficient gene editing in tomato in the first generation using the CRISPR/Cas9 system", PLANT PHYSIOLOGY, (201409), page 114
	- SHAN, "Targeted genome modification of crop plants using a CRISPR-Cas system", NATURE BIOTECHNOLOGY, (2013), vol. 31, pages 686 - 688
	- FENG, "Efficient genome editing in plants using a CRISPR/Cas system", CELL RESEARCH, (20130820), vol. 23, pages 1229 - 1232
	- XIE, "RNA-guided genome editing in plants using a CRISPR-Cas system", MOL PLANT., (20130817), vol. 6, no. 6, pages 1975 - 83
	- XU, "Gene targeting using the Agrobacterium tumefaciens-mediated CRISPR-Cas system in rice", RICE, (2014), vol. 7, page 5
	- ZHOU ET AL., "Exploiting SNPs for biallelic CRISPR mutations in the outcrossing woody perennial Populus reveals 4-coumarate: CoA ligase specificity and Redundancy", NEW PHYTOLOGIST, (2015), pages 1 - 4, URL: www.newphytologist.com
	- CALIANDO ET AL., "Targeted DNA degradation using a CRISPR device stably carried in the host genome", NATURE COMMUNICATIONS, vol. 6, page 6989, URL: www.nature.com/naturecommunications
	- MORRELL ET AL., "Crop genomics: advances and applications", NAT REV GENET., (20111229), vol. 13, no. 2, pages 85 - 96
	- SUGANO ET AL., PLANT CELL PHYSIOL., (20140118), vol. 55, no. 3, pages 475 - 81
	- KABADI ET AL., NUCLEIC ACIDS RES., (20140813), vol. 42, no. 19, page EL47
	- XING ET AL., BMC PLANT BIOLOGY, (2014), vol. 14, page 327
	- METHODS IN MOLECULAR BIOLOGY, (20150210), vol. 1284, pages 239 - 255
	- MA ET AL., MOL PLANT., (20150803), vol. 8, no. 8, pages 1274 - 84
	- LOWDER ET AL., PLANT PHYSIOL., (20150821), page 00636
	- STOVICEK ET AL., METAB. ENG. COMM., (2015), vol. 2, page 13
	- HEBELSTRUP ET AL., FRONT PLANT SCI., (20150423), vol. 6, page 247
	- KURTH, J VIROL., (20120321), vol. 86, no. 11, pages 6002 - 9
	- CURTIN ET AL., "A genome engineering toolbox for legume Functional genomics", NTERNATIONAL PLANT AND ANIMAL GENOME CONFERENCE, (2014), vol. XXII
	- NICOLAOU ET AL., CURRENT OPINION IN ALLERGY AND CLINICAL IMMUNOLOGY, (2011), vol. 11, no. 3, pages 222 - 228
	- BELHAJ ET AL., PLANT METHODS, vol. 9, page 39
	- HARRISON ET AL., GENES & DEVELOPMENT, vol. 28, pages 1859 - 1872
	- ZHOU ET AL., NEW PHYTOLOGIST, (201510), vol. 208, no. 2, pages 298 - 301
	- HUM. MOL. GENET., (2006), vol. 15, no. 2, pages R162 - R169
	- CELL, (20090220), vol. 136, no. 4, pages 777 - 793
	- CONG, L.; RAN, F.A.; COX, D.; LIN, S.; BARRETTO, R.; HABIB, N.; HSU, P.D.; WU, X.; JIANG, W.; MARRAFFINI, L.A., "Multiplex genome engineering using CRISPR/Cas systems.", SCIENCE, (20130215), vol. 339, no. 6121, pages 819 - 23
	- JIANG W.; BIKARD D.; COX D.; ZHANG F; MARRAFFINI LA, "RNA-guided editing of bacterial genomes using CRISPR-Cas systems", NAT BIOTECHNOL, (201303), vol. 31, no. 3, pages 233 - 9
	- WANG H.; YANG H.; SHIVALILA CS.; DAWLATY MM.; CHENG AW.; ZHANG F.; JAENISCH R., "One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering", CELL, (20130509), vol. 153, no. 4, pages 910 - 8
	- KONERMANN S; BRIGHAM MD; TREVINO AE; HSU PD; HEIDENREICH M; CONG L; PLATT RJ; SCOTT DA; CHURCH GM; ZHANG F, "Optical control of mammalian endogenous transcription and epigenetic states", NATURE, (20130823), vol. 500, no. 7463, pages 472 - 6
	- RAN, FA.; HSU, PD.; LIN, CY.; GOOTENBERG, JS.; KONERMANN, S.; TREVINO, AE.; SCOTT, DA.; INOUE, A.; MATOBA, S.; ZHANG, Y., "Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity", CELL, (20130828), pages 0092 - 8674
	- HSU, P.; SCOTT, D.; WEINSTEIN, J.; RAN, FA.; KONERMANN, S.; AGARWALA, V.; LI, Y.; FINE, E.; WU, X.; SHALEM, O., "DNA targeting specificity of RNA-guided Cas9 nucleases", NAT BIOTECHNOL
	- RAN, FA.; HSU, PD.; WRIGHT, J.; AGARWALA, V.; SCOTT, DA.; ZHANG, F., "jr Genome engineering using the CRISPR-Cas9 system", NATURE PROTOCOLS, (201311), vol. 8, no. 11, pages 2281 - 308
	- NISHIMASU, H.; RAN, FA.; HSU, PD.; KONERMANN, S.; SHEHATA, SI.; DOHMAE, N.; ISHITANI, R.; ZHANG, F.; NUREKI, O., "Crystal structure of cas9 in complex with guide RNA and target DNA", CELL, (20140227), vol. 156, no. 5, pages 935 - 49
	- PLATT RJ; CHEN S; ZHOU Y; YIM MJ; SWIECH L; KEMPTON HR; DAHLMAN JE; PARNAS O; EISENHAURE TM; JOVANOVIC M, "CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling", CELL, (2014), vol. 159, no. 2, pages 440 - 455
	- HSU PD; LANDER ES; ZHANG F, "Development and Applications of CRISPR-Cas9 for Genome Engineering", CELL, (20140605), vol. 157, no. 6, pages 1262 - 78
	- WANG T; WEI JJ; SABATINI DM; LANDER ES, "Genetic screens in human cells using the CRISPR/Cas9 system", SCIENCE, (20140103), vol. 343, no. 6166, pages 80 - 84
	- DOENCH JG; HARTENIAN E; GRAHAM DB; TOTHOVA Z; HEGDE M; SMITH I; SULLENDER M; EBERT BL; XAVIER RJ; ROOT DE, "Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation", NAT BIOTECHNOL., (20140903), vol. 32, no. 12, pages 1262 - 7
	- SWIECH L; HEIDENREICH M; BANERJEE A; HABIB N; LI Y; TROMBETTA J; SUR M; ZHANG F, "In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9", NAT BIOTECHNOL., (20141019), vol. 33, no. L, pages 102 - 6
	- KONERMANN S; BRIGHAM MD; TREVINO AE; JOUNG J; ABUDAYYEH 00; BARCENA C; HSU PD; HABIB N; GOOTENBERG JS; NISHIMASU H, "Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex", NATURE, (20150129), vol. 517, no. 7536, pages 583 - 8
	- ZETSCHE B; VOLZ SE; ZHANG F., "A split-Cas9 architecture for inducible genome editing and transcription modulation", NAT BIOTECHNOL., (20150202), vol. 33, no. 2, pages 139 - 42
	- CHEN S; SANJANA NE; ZHENG K; SHALEM O; LEE K; SHI X; SCOTT DA; SONG J; PAN JQ; WEISSLEDER R, "Genome-wide CRISPR Screen in a Mouse Model of Tumor Growth and Metastasis", CELL, (20150312), vol. 160, pages 1246 - 1260
	- RAN FA; CONG L; YAN WX; SCOTT DA; GOOTENBERG JS; KRIZ AJ; ZETSCHE B; SHALEM O; WU X; MAKAROVA KS, "In vivo genome editing using Staphylococcus aureus Cas9", NATURE, (20150401), vol. 520, no. 7546, pages 186 - 91
	- SHALEM ET AL., "High-throughput functional genomics using CRISPR-Cas9", NATURE REVIEWS GENETICS, (201505), vol. 16, pages 299 - 311
	- XU ET AL., "Sequence determinants of improved CRISPR sgRNA design", GENOME RESEARCH, (201508), vol. 25, pages 1147 - 1157
	- PARNAS ET AL., "A Genome-wide CRISPR Screen in Primary Immune Cells to Dissect Regulatory Networks", CELL, (20150730), vol. 162, pages 675 - 686
	- RAMANAN ET AL., "CRISPR/Cas9 cleavage of viral DNA efficiently suppresses hepatitis B virus", SCIENTIFIC REPORTS, (20150602), vol. 5, page 10833
	- NISHIMASU ET AL., "Crystal Structure of Staphylococcus aureus Cas9", CELL, (20150827), vol. 162, pages 1113 - 1126
	- ZETSCHE, "Cpfl Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System", CELL, (20151022), vol. 163, pages 1 - 13
	- SHMAKOV ET AL., "Discoverv and Functional Characterization of Diverse Class 2 CRISPR-Cas Svstems", MOLECULAR CELL, (20151022), vol. 60, pages 1 - 13
	WO1994US05700
	US20040491026
	US20090511940
	WO2014US70057

The EPO accepts no responsibility for the accuracy of data originating from other authorities; in particular, it does not guarantee that it is complete, up to date or fit for specific purposes.

Legal notice
Terms of use
24.1.24; 34p