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EP3694875
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| EP3694875 - ANTIBODIES TARGETING GLIOBLASTOMA STEM-LIKE CELLS AND METHODS OF USE THEREOF [Right-click to bookmark this link] | Status | Examination is in progress Status updated on 21.05.2021 Database last updated on 20.07.2024 | |
| Former | Request for examination was made Status updated on 17.07.2020 | ||
| Former | The international publication has been made Status updated on 19.04.2019 | ||
| Former | unknown Status updated on 27.11.2018 | Most recent event Tooltip | 21.10.2023 | New entry: Reply to examination report | Applicant(s) | For all designated states Wisconsin Alumni Research Foundation 614 Walnut Street Madison, WI 53726 / US | [2020/34] | Inventor(s) | 01 /
ZORNIAK, Michael 6134 N. Meade Avenue Chicago, IL 60646 / US | 02 /
CLARK, Paul, A. 600 Highland Avenue Madison, WI 53792 / US | 03 /
CHO, Yongku, Peter 191 Auditorium Road Unit 3222 Storrs, CT 06269-3222 / US | 04 /
UMLAUF, Benjamin, J. 1415 Engineering Drive Madison, WI 53706-1691 / US | 05 /
SHUSTA, Eric, V. 2720 Waunona Way Madison, WI 53706 / US | 06 /
KUO, John, Shu-Shin 3500 N. Capital of Texas Hwy Apt 512 Austin, TX 78746 / US | [2020/34] | Representative(s) | J A Kemp LLP 80 Turnmill Street London EC1M 5QU / GB | [N/P] |
| Former [2020/34] | J A Kemp LLP 14 South Square Gray's Inn London WC1R 5JJ / GB | Application number, filing date | 18803799.8 | 09.10.2018 | [2020/34] | WO2018US54952 | Priority number, date | US201762569834P | 09.10.2017 Original published format: US 201762569834 P | [2020/34] | Filing language | EN | Procedural language | EN | Publication | Type: | A1 Application with search report | No.: | WO2019074892 | Date: | 18.04.2019 | Language: | EN | [2019/16] | Type: | A1 Application with search report | No.: | EP3694875 | Date: | 19.08.2020 | Language: | EN | The application published by WIPO in one of the EPO official languages on 18.04.2019 takes the place of the publication of the European patent application. | [2020/34] | Search report(s) | International search report - published on: | EP | 18.04.2019 | Classification | IPC: | C07K16/18, C07K16/30, G01N33/53, A61P35/00, A61K39/00 | [2020/34] | CPC: |
C07K16/18 (EP);
C07K16/3053 (US);
A61K49/0058 (US);
A61P35/00 (EP);
C07K16/30 (EP);
G01N33/57407 (EP,US);
A61K2039/505 (EP,US);
C07K2317/565 (US);
C07K2317/569 (EP);
| Designated contracting states | AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LI, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR [2020/34] | Title | German: | ANTIKÖRPER GEGEN STAMMARTIGE GLIOBLASTOMZELLEN UND VERFAHREN ZU DEREN VERWENDUNG | [2020/34] | English: | ANTIBODIES TARGETING GLIOBLASTOMA STEM-LIKE CELLS AND METHODS OF USE THEREOF | [2020/34] | French: | ANTICORPS CIBLANT DES CELLULES DE TYPE SOUCHE DE GLIOBLASTOME ET LEURS PROCÉDÉS D'UTILISATION | [2020/34] | Entry into regional phase | 28.04.2020 | National basic fee paid | 28.04.2020 | Designation fee(s) paid | 28.04.2020 | Examination fee paid | Examination procedure | 28.04.2020 | Amendment by applicant (claims and/or description) | 28.04.2020 | Examination requested [2020/34] | 28.04.2020 | Date on which the examining division has become responsible | 20.05.2021 | Despatch of a communication from the examining division (Time limit: M04) | 17.09.2021 | Reply to a communication from the examining division | 27.07.2023 | Despatch of a communication from the examining division (Time limit: M04) | 20.10.2023 | Reply to a communication from the examining division | Fees paid | Renewal fee | 14.10.2020 | Renewal fee patent year 03 | 29.09.2021 | Renewal fee patent year 04 | 15.09.2022 | Renewal fee patent year 05 | 14.09.2023 | Renewal fee patent year 06 |
| Opt-out from the exclusive Tooltip competence of the Unified Patent Court | See the Register of the Unified Patent Court for opt-out data | ||
| Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court. | Cited in | International search | [A]WO2017120998 (BEIJING MARINO BIOTECHNOLOGY PTY LTD [CN]) [A] 1-41 * the whole document *; | [IDA] - X. ZHU ET AL, "Identification of Internalizing Human Single-Chain Antibodies Targeting Brain Tumor Sphere Cells", MOLECULAR CANCER THERAPEUTICS, (20100701), vol. 9, no. 7, doi:10.1158/1535-7163.MCT-09-1059, ISSN 1535-7163, pages 2131 - 2141, XP055178095 [ID] 1,2 * the whole document * [A] 3-41 DOI: http://dx.doi.org/10.1158/1535-7163.MCT-09-1059 | [AD] - J K LIU ET AL, "Phage display discovery of novel molecular targets in glioblastoma-initiating cells", CELL DEATH AND DIFFERENTIATION., GB, (20140516), vol. 21, no. 8, doi:10.1038/cdd.2014.65, ISSN 1350-9047, pages 1325 - 1339, XP055544885 [AD] 1-41 * the whole document * DOI: http://dx.doi.org/10.1038/cdd.2014.65 | [AD] - Y. KIM ET AL, "Aptamer Identification of Brain Tumor-Initiating Cells", CANCER RESEARCH, US, (20130624), vol. 73, no. 15, doi:10.1158/0008-5472.CAN-12-4556, ISSN 0008-5472, pages 4923 - 4936, XP055544884 [AD] 1-41 * the whole document * DOI: http://dx.doi.org/10.1158/0008-5472.CAN-12-4556 | [A] - SAMUEL BECK ET AL, "Identification of a peptide that interacts with Nestin protein expressed in brain cancer stem cells", BIOMATERIALS, ELSEVIER SCIENCE PUBLISHERS BV., BARKING, GB, vol. 32, no. 33, doi:10.1016/J.BIOMATERIALS.2011.07.048, ISSN 0142-9612, (20110716), pages 8518 - 8528, (20110801), XP028295853 [A] 1-41 * the whole document * DOI: http://dx.doi.org/10.1016/j.biomaterials.2011.07.048 | [A] - POINTER KELLI B ET AL, "Glioblastoma cancer stem cells: Biomarker and therapeutic advances", NEUROCHEMISTRY INTERNATIONAL, (20140319), vol. 71, doi:10.1016/J.NEUINT.2014.03.005, ISSN 0197-0186, pages 1 - 7, XP028655023 [A] 1-41 * the whole document * DOI: http://dx.doi.org/10.1016/j.neuint.2014.03.005 | [XP] - MICHAEL ZORNIAK ET AL, "Yeast display biopanning identifies human antibodies targeting glioblastoma stem-like cells", SCIENTIFIC REPORTS, (20171120), vol. 7, no. 1, doi:10.1038/s41598-017-16066-1, XP055544982 [XP] 1-41 * the whole document * DOI: http://dx.doi.org/10.1038/s41598-017-16066-1 | [AP] - ARNOLD AMY E ET AL, "Antibody-Antisense Oligonucleotide Conjugate Downregulates a Key Gene in Glioblastoma Stem Cells.", MOLECULAR THERAPY. NUCLEIC ACIDS 01 JUN 2018, (20180601), vol. 11, ISSN 2162-2531, pages 518 - 527, XP002788205 [AP] 1-41 * the whole document * DOI: http://dx.doi.org/10.1016/j.omtn.2018.04.004 | by applicant | US2008019984 | WO2012075037 | US2013287748 | WO2014033074 | WO2014099671 | WO2015101586 | US2015196663 | US2017174778 | - RODGERS et al., "Switch-mediated activation and retargeting of CAR-T cells for B-cell malignancies", PNAS, (20060112), pages E459 - E468 | - WU et al., "Remote control of therapeutic T cells through a small molecule-gated chimeric receptor", Science, (20151016), vol. 350, no. 6258, doi:doi:10.1126/science.aab4077, pages aab4077 - 9, XP055260068 DOI: http://dx.doi.org/10.1126/science.aab4077 | - VALTON et al., "A Versatile Safeguard for Chimeric Antigen Receptor T-cell Immunotherapies", Nature Scientific Reports, (20180000), vol. 8 | - KARLIN; ALTSCHUL, Proc. Natl. Acad. Sci. USA, (19900000), vol. 87, pages 2267 - 2268 | - ALTSCHUL et al., Nucl. Acids Res., (19970000), vol. 25, pages 3389 - 3402 | - ZHANG et al., "Co-stimulatory and Co-inhibitory Pathways in Autoimmunity", Immunity, (20160517), vol. 44, doi:doi:10.1016/j.immuni.2016.04.017, pages 1034 - 1051, XP029537983 DOI: http://dx.doi.org/10.1016/j.immuni.2016.04.017 | - GAVRILYUK et al., "3-Lactam-based Approach for Chemical Programming of Aldolast Antibody 38C2", Bioorg Med Chem Lett., (20090301), vol. 19, no. 5, pages 1421 - 1424 | - B. VAN DE BROEK et al., ACS Nano, (20110000), vol. 5, no. 6, pages 4319 - 4328 | - A. SUKHANOVA et al., Nanomedicine, (20120000), vol. 8, pages 516 - 525 | - SUGANO et al., "Antibody Targeting of Doxorubicin-loaded Liposomes Suppresses the Growth and Metastatic Spread of Established Human Lung Tumor Xenografts in Severe Combined Immunodeficient Mice", Cancer Research, (20001215), vol. 60, pages 6942 - 6949, XP000980029 | - MARTIN et al., "Nanomaterials in Analytical Chemistry", Analytical Chemistry News & Features, (19980501), pages 322 A - 327 A | - JONES, A.R.; E.V. SHUSTA, "Blood-brain barrier transport of therapeutics via receptor-mediation", Pharmaceutical Research, (20070000), vol. 24, no. 9, doi:doi:10.1007/s11095-007-9379-0, pages 1759 - 1771, XP019532601 DOI: http://dx.doi.org/10.1007/s11095-007-9379-0 | - Merck Index | - Physicians' Desk Reference | - The Pharmacological Basis of Therapeutics | - MINNITI et al., "Chemotherapy for Glioblastoma: Current Treatment and Further Perspectives for Cytotoxic and Targeted Agents", Anticancer Research, (20091200), vol. 29, no. 12, pages 5171 - 5184 | - STUPP, R. et al., "Radiotherapy plus concomitant and adjuvant temozolomide for glioblastoma", NEngl J Med, (20050000), vol. 352, doi:doi:10.1056/NEJMoa043330, pages 987 - 996, XP002439490 DOI: http://dx.doi.org/10.1056/NEJMoa043330 | - STUPP, R. et al., "Maintenance Therapy With Tumor-Treating Fields Plus Temozolomide vs Temozolomide Alone for Glioblastoma: A Randomized Clinical Trial", JAMA : the journal of the American Medical Association, (20150000), vol. 314, pages 2535 - 2543 | - BAO, S. et al., "Glioma stem cells promote radioresistance by preferential activation of the DNA damage response", Nature, (20060000), vol. 444, doi:doi:10.1038/nature05236, pages 756 - 760, XP055200572 DOI: http://dx.doi.org/10.1038/nature05236 | - LIU, G. et al., "Analysis of gene expression and chemoresistance of CD133+ cancer stem cells in glioblastoma", Molecular cancer, (20060000), vol. 5, doi:doi:10.1186/1476-4598-5-67, page 67, XP021024382 DOI: http://dx.doi.org/10.1186/1476-4598-5-67 | - CLARK, P. A. et al., "Activation of multiple ERBB family receptors mediates glioblastoma cancer stem-like cell resistance to EGFR-targeted inhibition", Neoplasia, (20120000), vol. 14, pages 420 - 428 | - SINGH, S. K. et al., "Identification of human brain tumour initiating cells", Nature, (20040000), vol. 432, doi:doi:10.1038/nature03128, pages 396 - 401, XP002467853 DOI: http://dx.doi.org/10.1038/nature03128 | - SON, M. J.; WOOLARD, K.; NAM, D. H.; LEE, J.; FINE, H. A., "SSEA-1 is an enrichment marker for tumor-initiating cells in human glioblastoma", Cell stem cell, (20090000), vol. 4, pages 440 - 452 | - CLEMENT, V. et al., "Marker-independent identification of glioma-initiating cells", Nature methods, (20100000), vol. 7, doi:doi:10.1038/nmeth.1430, pages 224 - 228, XP007914533 DOI: http://dx.doi.org/10.1038/nmeth.1430 | - CHEN, R. et al., "A hierarchy of self-renewing tumor-initiating cell types in glioblastoma", Cancer cell, (20100000), vol. 17, doi:doi:10.1016/j.ccr.2009.12.049, pages 362 - 375, XP055032071 DOI: http://dx.doi.org/10.1016/j.ccr.2009.12.049 | - POINTER, K. B.; CLARK, P. A.; ZORNIAK, M.; ALRFAEI, B. M.; KUO, J. S., "Glioblastoma cancer stem cells: Biomarker and therapeutic advances", Neurochem Int, (20140000), vol. 71, doi:doi:10.1016/j.neuint.2014.03.005, pages 1 - 7, XP028655023 DOI: http://dx.doi.org/10.1016/j.neuint.2014.03.005 | - ZHU, X. et al., "Identification of internalizing human single-chain antibodies targeting brain tumor sphere cells", Molecular cancer therapeutics, (20100000), vol. 9, doi:doi:10.1158/1535-7163.MCT-09-1059, pages 2131 - 2141, XP055178095 DOI: http://dx.doi.org/10.1158/1535-7163.MCT-09-1059 | - BECK, S. et al., "Identification of a peptide that interacts with Nestin protein expressed in brain cancer stem cells", Biomaterials, (20110000), vol. 32, doi:doi:10.1016/j.biomaterials.2011.07.048, pages 8518 - 8528, XP028295853 DOI: http://dx.doi.org/10.1016/j.biomaterials.2011.07.048 | - KIM, Y. et al., "Aptamer Identification of Brain Tumor-Initiating Cells", Cancer research, (20130000), vol. 73, pages 4923 - 4936 | - FELDHAUS, M. J. et al., "Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library", Nature biotechnology, (20030000), vol. 21, pages 163 - 170, XP002451748 | - SHUSTA, E. V.; HOLLER, P. D.; KIEKE, M. C.; KRANZ, D. M.; WITTRUP, K. D., "Directed evolution of a stable scaffold for T-cell receptor engineering", Nature biotechnology, (20000000), vol. 18, doi:doi:10.1038/77325, pages 754 - 759, XP055004062 DOI: http://dx.doi.org/10.1038/77325 | - RICHMAN, S. A. et al., "Development of a novel strategy for engineering high-affinity proteins by yeast display", Protein engineering, design & selection : PEDS, (20060000), vol. 19, pages 255 - 264 | - WANG, X. X.; SHUSTA, E. V., "The use of scFv-displaying yeast in mammalian cell surface selections", Journal of immunological methods, (20050000), vol. 304, pages 30 - 42 | - WANG, X. X.; CHO, Y. K.; SHUSTA, E. V., "Mining a yeast library for brain endothelial cell-binding antibodies", Nature methods, (20070000), vol. 4, doi:doi:10.1038/nmeth993, pages 143 - 145, XP008086824 DOI: http://dx.doi.org/10.1038/nmeth993 | - BODER, E. T.; WITTRUP, K. D., "Yeast surface display for directed evolution of protein expression, affinity, and stability", Methods in enzymology, (20000000), vol. 328, doi:doi:10.1016/S0076-6879(00)28410-3, pages 430 - 444, XP009103962 DOI: http://dx.doi.org/10.1016/S0076-6879(00)28410-3 | - LIU, H.; STYLES, C. A.; FINK, G. R., "Saccharomyces cerevisiae S288C has a mutation in FL08, a gene required for filamentous growth", Genetics, (19960000), vol. 144, pages 967 - 978 | - CLARK, P. A.; TREISMAN, D. M.; EBBEN, J.; KUO, J. S., "Developmental signaling pathways in brain tumor-derived stem-like cells", Dev Dyn, (20070000), vol. 236, pages 3297 - 3308 | - ZORNIAK, M. et al., "Differential expression of 2',3'-cyclic-nucleotide 3'-phosphodiesterase and neural lineage markers correlate with glioblastoma xenograft infiltration and patient survival", Clinical cancer research : an official journal of the American Association for Cancer Research, (20120000), vol. 18, pages 3628 - 3636 | - WEICHERT, J. P. et al., "Alkylphosphocholine analogs for broad-spectrum cancer imaging and therapy", Science translational medicine, (20140000), vol. 6, page 240ra275 | - ZORNIAK, M.; CLARK, P. A.; KUO, J. S., "Myelin-forming cell-specific cadherin-19 is a marker for minimally infiltrative glioblastoma stem-like cells", J Neurosurg, (20150000), vol. 122, pages 69 - 77 | - CLARK, P. A. et al., "Resveratrol targeting of AKT and p53 in glioblastoma and glioblastoma stem-like cells to suppress growth and infiltration", J Neurosurg, (20160000), pages 1 - 13 | - POLLARD, S. M. et al., "Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are suitable for chemical and genetic screens", Cell stem cell, (20090000), vol. 4, doi:doi:10.1016/j.stem.2009.03.014, pages 568 - 580, XP002665238 DOI: http://dx.doi.org/10.1016/j.stem.2009.03.014 | - CHO, Y. K.; CHEN, I.; WEI, X.; LI, L.; SHUSTA, E. V., "A yeast display immunoprecipitation method for efficient isolation and characterization of antigens", Journal of immunological methods, (20090000), vol. 341, doi:doi:10.1016/j.jim.2008.11.005, pages 117 - 126, XP025913977 DOI: http://dx.doi.org/10.1016/j.jim.2008.11.005 | - VANANTWERP, J. J.; WITTRUP, K. D., "Fine affinity discrimination by yeast surface display and flow cytometry", Biotechnology progress, (20000000), vol. 16, pages 31 - 37 | - SHUSTA, E. V.; RAINES, R. T.; PLUCKTHUN, A.; WITTRUP, K. D., "Increasing the secretory capacity of Saccharomyces cerevisiae for production of single-chain antibody fragments", Nature biotechnology, (19980000), vol. 16, doi:doi:10.1038/nbt0898-773, pages 773 - 777, XP002138520 DOI: http://dx.doi.org/10.1038/nbt0898-773 | - LIU, J. K. et al., "Phage display discovery of novel molecular targets in glioblastoma-initiating cells", Cell Death Differ, (20140000), vol. 21, pages 1325 - 1339 | - LEE, J. et al., "Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines", Cancer cell, (20060000), vol. 9, doi:doi:10.1016/j.ccr.2006.03.030, pages 391 - 403, XP002550739 DOI: http://dx.doi.org/10.1016/j.ccr.2006.03.030 | - HOMMA, T. et al., "Correlation among pathology, genotype, and patient outcomes in glioblastoma", Journal of neuropathology and experimental neurology, (20060000), vol. 65, pages 846 - 854, XP009132036 | - HOOGENBOOM, H. R., "Selecting and screening recombinant antibody libraries", Nature biotechnology, (20050000), vol. 23, doi:doi:10.1038/nbt1126, pages 1105 - 1116, XP002348401 DOI: http://dx.doi.org/10.1038/nbt1126 | - LIPOVSEK, D. et al., "Selection of horseradish peroxidase variants with enhanced enantioselectivity by yeast surface display", Chemistry & biology, (20070000), vol. 14, doi:doi:10.1016/j.chembiol.2007.09.008, pages 1176 - 1185, XP009091881 DOI: http://dx.doi.org/10.1016/j.chembiol.2007.09.008 | - ACKERMAN, M. et al., "Highly avid magnetic bead capture: an efficient selection method for de novo protein engineering utilizing yeast surface display", Biotechnology progress, (20090000), vol. 25, pages 774 - 783 | - CHOI, B. D. et al., "Systemic administration of a bispecific antibody targeting EGFRvIII successfully treats intracerebral glioma", Proceedings of the National Academy of Sciences of the United States of America, (20130000), vol. 110, doi:doi:10.1073/pnas.1219817110, pages 270 - 275, XP055156305 DOI: http://dx.doi.org/10.1073/pnas.1219817110 | - LOCKMAN, P. R. et al., "Heterogeneous blood-tumor barrier permeability determines drug efficacy in experimental brain metastases of breast cancer", Clinical cancer research : an official journal of the American Association for Cancer Research, (20100000), vol. 16, pages 5664 - 5678 | - SWANSON, K. I. et al., "Fluorescent cancer-selective alkylphosphocholine analogs for intraoperative glioma detection", Neurosurgery, (20150000), vol. 76, pages 115 - 123 | - CLARK, P. A. et al., "Analysis of Cancer-Targeting Alkylphosphocholine Analogue Permeability Characteristics Using a Human Induced Pluripotent Stem Cell Blood-Brain Barrier Model", Mol Pharm, (20160000), vol. 13, pages 3341 - 3349 | - ZHANG, R. R.; SWANSON, K. I.; HALL, L. T.; WEICHERT, J. P.; KUO, J. S., "Diapeutic cancer-targeting alkylphosphocholine analogs may advance management of brain malignancies", CNS Oncol, (20160000), vol. 5, pages 223 - 231 | - PARDRIDGE, W. M., "Re-engineering therapeutic antibodies for Alzheimer's disease as blood-brain barrier penetrating bi-specific antibodies", Expert Opin Biol Ther, (20160000), pages 1 - 14 | - PARDRIDGE, W. M., "CSF, blood-brain barrier, and brain drug delivery", Expert Opin Drug Deliv, (20160000), vol. 13, pages 963 - 975 | - LIU, H. L. et al., "Focused Ultrasound Enhances Central Nervous System Delivery of Bevacizumab for Malignant Glioma Treatment", Radiology, (20160000), vol. 281, pages 99 - 108 | - RAPOPORT, S. I., "Osmotic opening of the blood-brain barrier: principles, mechanism, and therapeutic applications", Cellular and molecular neurobiology, (20000000), vol. 20, pages 217 - 230 | - LIU, R.; MARTUZA, R. L.; RABKIN, S. D., "Intracarotid delivery of oncolytic HSV vector G47Delta to metastatic breast cancer in the brain", Gene therapy, (20050000), vol. 12, pages 647 - 654 | - YANG, Y. et al., "Targeting CD 146 with a 64Cu-labeled antibody enables in vivo immunoPET imaging of high-grade gliomas", Proceedings of the National Academy of Sciences of the United States of America, (20150000), vol. 112, doi:doi:10.1073/pnas.1502648112, pages E6525 - 6534, XP055453695 DOI: http://dx.doi.org/10.1073/pnas.1502648112 | - HAMBLETT, K. J. et al., "AMG 595, an Anti-EGFRvIII Antibody-Drug Conjugate, Induces Potent Antitumor Activity against EGFRvIII-Expressing Glioblastoma", Molecular cancer therapeutics, (20150000), vol. 14, pages 1614 - 1624 | - SURYADEVARA, C. M. et al., "Are BiTEs the ''missing link'' in cancer therapy?", Oncoimmunology, (20150000), vol. 4, page el008339 | - KRENCIUTE, G. et al., "Characterization and Functional Analysis of scFv-based Chimeric Antigen Receptors to Redirect T Cells to IL13Ralpha2-positive Glioma", Mol Ther, (20160000), vol. 24, doi:doi:10.1038/mt.2015.199, pages 354 - 363, XP055462357 DOI: http://dx.doi.org/10.1038/mt.2015.199 | - SHIINA, S. et al., "CAR T Cells Targeting Podoplanin Reduce Orthotopic Glioblastomas in Mouse Brains", Cancer Immunol Res, (20160000), vol. 4, pages 259 - 268 | - SVENDSEN, C. N. et al., "A new method for the rapid and long term growth of human neural precursor cells", JNeurosci Methods, (19980000), vol. 85, doi:doi:10.1016/S0165-0270(98)00126-5, pages 141 - 152, XP002902582 DOI: http://dx.doi.org/10.1016/S0165-0270(98)00126-5 | - IGNATOVA, T. N. et al., "Human cortical glial tumors contain neural stem-like cells expressing astroglial and neuronal markers in vitro", Glia, (20020000), vol. 39, doi:doi:10.1002/glia.10094, pages 193 - 206, XP009044599 DOI: http://dx.doi.org/10.1002/glia.10094 | - SINGH, S. K. et al., "Identification of a cancer stem cell in human brain tumors", Cancer research, (20030000), vol. 63, pages 5821 - 5828 | - HACKEL, B. J.; HUANG, D.; BUBOLZ, J. C.; WANG, X. X.; SHUSTA, E. V., "Production of soluble and active transferrin receptor-targeting single-chain antibody using Saccharomyces cerevisiae", Pharmaceutical research, (20060000), vol. 23, doi:doi:10.1007/s11095-006-9778-7, pages 790 - 797, XP019405238 DOI: http://dx.doi.org/10.1007/s11095-006-9778-7 | - MARSHALL, C. J. et al., "An evolved Mxe GyrA intein for enhanced production of fusion proteins", ACS Chem Biol, (20150000), vol. 10, pages 527 - 538 | - HUANG, D.; GORE, P. R.; SHUSTA, E. V., "Increasing yeast secretion of heterologous proteins by regulating expression rates and post-secretory loss", Biotechnol Bioeng, (20080000), vol. 101, pages 1264 - 1275 | - BUMS, M. L. et al., "Directed evolution of brain-derived neurotrophic factor for improved folding and expression in Saccharomyces cerevisiae", Applied and environmental microbiology, (20140000), vol. 80, pages 5732 - 5742 | - TILLOTSON, B. J.; CHO, Y. K.; SHUSTA, E. V., "Cells and cell lysates: a direct approach for engineering antibodies against membrane proteins using yeast surface display", Methods, (20130000), vol. 60, pages 27 - 37 | - GALLI, R. et al., "Isolation and characterization of tumorigenic, stem-like neural precursors from human glioblastoma", Cancer research, (20040000), vol. 64, doi:doi:10.1158/0008-5472.CAN-04-1364, pages 7011 - 7021, XP002550845 DOI: http://dx.doi.org/10.1158/0008-5472.CAN-04-1364 | - YANG, Y. et al., "In vivo near-infrared fluorescence imaging of CD105 expression during tumor angiogenesis", European journal of nuclear medicine and molecular imaging, (20110000), vol. 38, doi:doi:10.1007/s00259-011-1886-x, pages 2066 - 2076, XP019961557 DOI: http://dx.doi.org/10.1007/s00259-011-1886-x | WO2007US70587 |