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Extract from the Register of European Patents

EP About this file: EP3701250

EP3701250 - FLUORESCENT DYE ACCUMULATION ASSAY [Right-click to bookmark this link]
StatusExamination is in progress
Status updated on  22.03.2024
Database last updated on 13.09.2024
FormerRequest for examination was made
Status updated on  31.07.2020
FormerThe international publication has been made
Status updated on  04.05.2019
Most recent event   Tooltip26.07.2024Change: Despatch of examination report + time limit 
Applicant(s)For all designated states
Cellpharma Kft.
Karolina út 29-31.
1113 Budapest / HU
For all designated states
Természettudományi Kutatóközpont
Magyar tudósok körútja 2
1117 Budapest / HU
[2020/36]
Inventor(s)01 / SZABÓ, Edit
Ady Endre út 67.
2. ajtó
1221 Budapest / HU
02 / KOVÁCS-TÜRK, Dóra
Meredek u. 28.
1. emelet 5. ajtó
1112 Budapest / HU
03 / TELBISZ, Ágnes
Bíró László u. 22.
1089 Budapest / HU
04 / KUCSMA, Nóra
Báthori u. 7.
1191 Budapest / HU
05 / HORVÁTH, Tamás
Köves út 82.
1237 Budapest / HU
06 / SZAKÁCS, Gergely
1026 Budapest
Nyúl u. 4.
Budapest / HU
07 / HOMOLYA, László
Áfonya köz 13.
2089 Telki / HU
08 / SARKADI, Balázs
Ágnes út 23/B.
1121 Budapest / HU
09 / VÁRADY, György
Báthori u. 7.
1191 Budapest / HU
 [2020/36]
Representative(s)Danubia Patent & Law Office LLC
Bajcsy-Zsilinszky út 16
1051 Budapest / HU
[2020/36]
Application number, filing date18870920.825.10.2018
[2020/36]
WO2018HU50046
Priority number, dateHU2017000043225.10.2017         Original published format: HU 1700432
[2020/36]
Filing languageEN
Procedural languageEN
PublicationType: A1 Application with search report
No.:WO2019081957
Date:02.05.2019
Language:EN
[2019/18]
Type: A1 Application with search report 
No.:EP3701250
Date:02.09.2020
Language:EN
The application published by WIPO in one of the EPO official languages on 02.05.2019 takes the place of the publication of the European patent application.
[2020/36]
Search report(s)International search report - published on:XV02.05.2019
(Supplementary) European search report - dispatched on:EP11.08.2021
ClassificationIPC:G01N21/64, G01N33/60, G01N33/58, G01N33/50, C07K14/00
[2020/36]
CPC:
C07K14/705 (EP,US); C07K14/00 (HU); G01N33/582 (EP,US);
C12P17/06 (US); G01N21/64 (HU,US); G01N33/5035 (EP,US);
G01N33/60 (HU,US); G01N2800/52 (EP,US); G01N2800/7028 (EP,US) (-)
Designated contracting statesAL,   AT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   LV,   MC,   MK,   MT,   NL,   NO,   PL,   PT,   RO,   RS,   SE,   SI,   SK,   SM,   TR [2020/36]
TitleGerman:FLUORESZENZFARBSTOFF-AKKUMULATIONSTEST[2020/36]
English:FLUORESCENT DYE ACCUMULATION ASSAY[2020/36]
French:DOSAGE D'ACCUMULATION DE COLORANT FLUORESCENT[2020/36]
Entry into regional phase25.05.2020National basic fee paid 
25.05.2020Search fee paid 
25.05.2020Designation fee(s) paid 
25.05.2020Examination fee paid 
Examination procedure25.08.2019Request for preliminary examination filed
International Preliminary Examining Authority: XV
25.05.2020Examination requested  [2020/36]
10.03.2022Amendment by applicant (claims and/or description)
26.03.2024Despatch of a communication from the examining division (Time limit: M06)
Fees paidRenewal fee
25.05.2020Renewal fee patent year 03
01.10.2021Renewal fee patent year 04
19.09.2022Renewal fee patent year 05
07.09.2023Renewal fee patent year 06
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Documents cited:Search[Y]WO2011139634  (ENZO LIFE SCIENCES INC [US], et al) [Y] 1-15 * table 1; claim 4 *;
 [Y]  - STROUSE J JACOB ET AL, "Fluorescent substrates for flow cytometric evaluation of efflux inhibition in ABCB1, ABCC1, and ABCG2 transporters", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, AMSTERDAM, NL, (20130305), vol. 437, no. 1, doi:10.1016/J.AB.2013.02.018, ISSN 0003-2697, pages 77 - 87, XP028545924 [Y] 1-15 * abstract; tables 1,2; fig. 1 *

DOI:   http://dx.doi.org/10.1016/j.ab.2013.02.018
 [XP]  - SZABÓ EDIT ET AL, "A new fluorescent dye accumulation assay for parallel measurements of the ABCG2, ABCB1 and ABCC1 multidrug transporter functions", PLOS ONE, (20180117), vol. 13, no. 1, doi:10.1371/journal.pone.0190629, page e0190629, XP055824472 [XP] 1-15 * the whole document *

DOI:   http://dx.doi.org/10.1371/journal.pone.0190629
International search[A]HUP0002557  (SOLVO BIOTECHNOLOGIAI KFT [HU]) [A] 1-29* Abstract and pages 4-10 *;
 [XA]WO2009052183  (INVITROGEN CORP [US], et al) [X] 26-27a and 28-29 * Paragraphs [0011-0013], [0049] and claims 72 and 73 * [A] 1-25 and 27b;
 [A]  - Iain Johnson (ed.), "Chapter 19: Indicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions", The Molecular Probes® Handbook, A GUIDE TO FLUORESCENT PROBES AND LABELING TECHNOLOGIES, (20100000), pages 829 - 882, ISBN 978-0-9829279-1-5, XP009520829 [A] 1-29
 [A]  - HORNOLYA L. et al., "Fluorescent Cellular Indicators Ate Extruded by the Multidrug Resistance Protein", J Biol Chem., (19931015), vol. 268, no. 29, pages 21493 - 6, XP055073841 [A] 1-29 * Abstract and page 21496 cited by the application *
The EPO accepts no responsibility for the accuracy of data originating from other authorities; in particular, it does not guarantee that it is complete, up to date or fit for specific purposes.