blank Quick help
blank Maintenance news

Scheduled maintenance

Regular maintenance outages:
between 05.00 and 05.15 hrs CET (Monday to Sunday).

Other outages
Availability

2022.02.11

More...
blank News flashes

News Flashes

New version of the European Patent Register – SPC proceedings information in the Unitary Patent Register.

2024-07-24

More...
blank Related links

Release notes
European Patent Register Assistant
European Patent Register Resource Book
European Patent Register – pocket guide
FAQ - European Patent Register
FAQ - Register Alert
Federated Register (Flyer)
Global Dossier (Flyer)
Register events
Deep link coverage
Federated Register content
The Patent Information Tour
Fair use charter

Extract from the Register of European Patents

EP About this file: EP3704248

EP3704248 - METHODS AND KITS FOR TARGETED ENRICHMENT OF TARGET DNA WITH HIGH GC CONTENT [Right-click to bookmark this link]
StatusNo opposition filed within time limit
Status updated on  01.09.2023
Database last updated on 14.09.2024
FormerThe patent has been granted
Status updated on  23.09.2022
FormerGrant of patent is intended
Status updated on  27.06.2022
FormerExamination is in progress
Status updated on  28.01.2022
FormerRequest for examination was made
Status updated on  07.08.2020
FormerThe international publication has been made
Status updated on  11.05.2019
Most recent event   Tooltip24.05.2024Lapse of the patent in a contracting state
New state(s): IT		published on 26.06.2024  [2024/26]
Applicant(s)For all designated states
Berry Genomics Co., Ltd
Room 801, Floor 8, Building No. 5, Yard 4
Shengmingyuan Road
Science Park
Changping District
Beijing 102206 / CN
[2022/43]
Former [2020/37]For all designated states
Berry Genomics Co., Ltd.
Room 801, Floor 8, Building No. 5, Yard 4
Shengmingyuan Road
Science Park
Changping District
Beijing 102206 / CN
Inventor(s)01 / QU, Ziwei
Room 801 Floor 8 Building No. 5
Yard 4
Shengmingyuan Road
Science Park, Changping District
Beijing 102206 / CN
02 / YU, Tao
Room 801 Floor 8 Building No. 5
Yard 4
Shengmingyuan Road
Science Park, Changping District
Beijing 102206 / CN
03 / WANG, Fangming
Room 801 Floor 8 Building No. 5
Yard 4
Shengmingyuan Road
Science Park, Changping District
Beijing 102206 / CN
04 / ZHANG, Nannan
Room 801 Floor 8 Building No. 5
Yard 4
Shengmingyuan Road
Science Park, Changping District
Beijing 102206 / CN
05 / ZHANG, Jianguang
Room 801 Floor 8 Building No. 5
Yard 4
Shengmingyuan Road
Science Park, Changping District
Beijing 102206 / CN
 [2020/37]
Representative(s)Plasseraud IP
104 Rue de Richelieu
CS92104
75080 Paris Cedex 02 / FR
[N/P]
Former [2020/37]Plasseraud IP
66, rue de la Chaussée d'Antin
75440 Paris Cedex 09 / FR
Application number, filing date18874699.407.02.2018
[2020/37]
WO2018CN75543
Priority number, dateCN2017117077603.11.2017         Original published format: CN201711070776
[2020/37]
Filing languageEN
Procedural languageEN
PublicationType: A1 Application with search report
No.:WO2019085320
Date:09.05.2019
Language:EN
[2019/19]
Type: A1 Application with search report 
No.:EP3704248
Date:09.09.2020
Language:EN
The application published by WIPO in one of the EPO official languages on 09.05.2019 takes the place of the publication of the European patent application.
[2020/37]
Type: B1 Patent specification 
No.:EP3704248
Date:26.10.2022
Language:EN
[2022/43]
Search report(s)International search report - published on:CN09.05.2019
(Supplementary) European search report - dispatched on:EP09.07.2021
ClassificationIPC:C12N15/10, C12Q1/68, C12Q1/6834, C12Q1/6827
[2021/32]
CPC:
C12Q1/6806 (EP,US); C12Q1/6827 (EP); C12Q1/6834 (EP);
C12Q1/6869 (US); C12Q2537/159 (US); C12Q2565/519 (US)
C-Set:
C12Q1/6806, C12Q2537/159, C12Q2565/519 (EP);
C12Q1/6827, C12Q2521/301, C12Q2521/514, C12Q2525/151, C12Q2527/107, C12Q2531/119, C12Q2565/518 (EP);
C12Q1/6834, C12Q2521/301, C12Q2521/514, C12Q2525/151, C12Q2527/107, C12Q2531/119, C12Q2565/518 (EP)
Former IPC [2020/37]C12N15/10, C12Q1/68
Designated contracting statesAL,   AT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   LV,   MC,   MK,   MT,   NL,   NO,   PL,   PT,   RO,   RS,   SE,   SI,   SK,   SM,   TR [2020/37]
Extension statesBANot yet paid
MENot yet paid
Validation statesMANot yet paid
MDNot yet paid
TNNot yet paid
TitleGerman:VERFAHREN UND KITS ZUR GEZIELTEN ANREICHERUNG VON ZIEL-DNA MIT HOHEM GC-GEHALT[2020/37]
English:METHODS AND KITS FOR TARGETED ENRICHMENT OF TARGET DNA WITH HIGH GC CONTENT[2020/37]
French:PROCÉDÉS ET KITS D'ENRICHISSEMENT CIBLÉ D'ADN CIBLE AVEC UNE TENEUR EN GC ÉLEVÉE[2020/37]
Entry into regional phase15.05.2020National basic fee paid 
15.05.2020Search fee paid 
15.05.2020Designation fee(s) paid 
15.05.2020Examination fee paid 
Examination procedure15.05.2020Amendment by applicant (claims and/or description)
15.05.2020Examination requested  [2020/37]
27.01.2022Despatch of a communication from the examining division (Time limit: M04)
12.05.2022Reply to a communication from the examining division
28.06.2022Communication of intention to grant the patent
16.09.2022Fee for grant paid
16.09.2022Fee for publishing/printing paid
16.09.2022Receipt of the translation of the claim(s)
Opposition(s)27.07.2023No opposition filed within time limit [2023/40]
Fees paidRenewal fee
15.05.2020Renewal fee patent year 03
13.01.2021Renewal fee patent year 04
13.01.2022Renewal fee patent year 05
Opt-out from the exclusive  Tooltip
competence of the Unified
Patent Court		 See the Register of the Unified Patent Court for opt-out data
Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Lapses during opposition  TooltipAL26.10.2022
AT26.10.2022
CZ26.10.2022
DK26.10.2022
EE26.10.2022
ES26.10.2022
FI26.10.2022
HR26.10.2022
IT26.10.2022
LT26.10.2022
LV26.10.2022
MC26.10.2022
NL26.10.2022
PL26.10.2022
RO26.10.2022
RS26.10.2022
SE26.10.2022
SI26.10.2022
SK26.10.2022
SM26.10.2022
NO26.01.2023
GR27.01.2023
IE07.02.2023
LU07.02.2023
IS26.02.2023
PT27.02.2023
BE28.02.2023
CH28.02.2023
FR28.02.2023
LI28.02.2023
[2024/26]
Former [2024/11]AL26.10.2022
AT26.10.2022
CZ26.10.2022
DK26.10.2022
EE26.10.2022
ES26.10.2022
FI26.10.2022
HR26.10.2022
LT26.10.2022
LV26.10.2022
MC26.10.2022
NL26.10.2022
PL26.10.2022
RO26.10.2022
RS26.10.2022
SE26.10.2022
SI26.10.2022
SK26.10.2022
SM26.10.2022
NO26.01.2023
GR27.01.2023
IE07.02.2023
LU07.02.2023
IS26.02.2023
PT27.02.2023
BE28.02.2023
CH28.02.2023
FR28.02.2023
LI28.02.2023
Former [2024/08]AL26.10.2022
AT26.10.2022
CZ26.10.2022
DK26.10.2022
EE26.10.2022
ES26.10.2022
FI26.10.2022
HR26.10.2022
LT26.10.2022
LV26.10.2022
MC26.10.2022
NL26.10.2022
PL26.10.2022
RO26.10.2022
RS26.10.2022
SE26.10.2022
SI26.10.2022
SK26.10.2022
SM26.10.2022
NO26.01.2023
GR27.01.2023
IE07.02.2023
LU07.02.2023
IS26.02.2023
PT27.02.2023
CH28.02.2023
LI28.02.2023
Former [2023/51]AL26.10.2022
AT26.10.2022
CZ26.10.2022
DK26.10.2022
EE26.10.2022
ES26.10.2022
FI26.10.2022
HR26.10.2022
LT26.10.2022
LV26.10.2022
MC26.10.2022
NL26.10.2022
PL26.10.2022
RO26.10.2022
RS26.10.2022
SE26.10.2022
SI26.10.2022
SK26.10.2022
SM26.10.2022
NO26.01.2023
GR27.01.2023
LU07.02.2023
IS26.02.2023
PT27.02.2023
CH28.02.2023
LI28.02.2023
Former [2023/49]AL26.10.2022
AT26.10.2022
CZ26.10.2022
DK26.10.2022
EE26.10.2022
ES26.10.2022
FI26.10.2022
HR26.10.2022
LT26.10.2022
LV26.10.2022
MC26.10.2022
NL26.10.2022
PL26.10.2022
RO26.10.2022
RS26.10.2022
SE26.10.2022
SK26.10.2022
SM26.10.2022
NO26.01.2023
GR27.01.2023
LU07.02.2023
IS26.02.2023
PT27.02.2023
CH28.02.2023
LI28.02.2023
Former [2023/48]AL26.10.2022
AT26.10.2022
CZ26.10.2022
DK26.10.2022
EE26.10.2022
ES26.10.2022
FI26.10.2022
HR26.10.2022
LT26.10.2022
LV26.10.2022
MC26.10.2022
NL26.10.2022
PL26.10.2022
RO26.10.2022
RS26.10.2022
SE26.10.2022
SK26.10.2022
SM26.10.2022
NO26.01.2023
GR27.01.2023
IS26.02.2023
PT27.02.2023
CH28.02.2023
LI28.02.2023
Former [2023/44]AL26.10.2022
AT26.10.2022
CZ26.10.2022
DK26.10.2022
EE26.10.2022
ES26.10.2022
FI26.10.2022
HR26.10.2022
LT26.10.2022
LV26.10.2022
MC26.10.2022
NL26.10.2022
PL26.10.2022
RO26.10.2022
RS26.10.2022
SE26.10.2022
SK26.10.2022
SM26.10.2022
NO26.01.2023
GR27.01.2023
IS26.02.2023
PT27.02.2023
Former [2023/38]AL26.10.2022
AT26.10.2022
CZ26.10.2022
DK26.10.2022
EE26.10.2022
ES26.10.2022
FI26.10.2022
HR26.10.2022
LT26.10.2022
LV26.10.2022
NL26.10.2022
PL26.10.2022
RO26.10.2022
RS26.10.2022
SE26.10.2022
SK26.10.2022
SM26.10.2022
NO26.01.2023
GR27.01.2023
IS26.02.2023
PT27.02.2023
Former [2023/37]AL26.10.2022
AT26.10.2022
CZ26.10.2022
DK26.10.2022
EE26.10.2022
ES26.10.2022
FI26.10.2022
HR26.10.2022
LT26.10.2022
LV26.10.2022
NL26.10.2022
PL26.10.2022
RO26.10.2022
RS26.10.2022
SE26.10.2022
SM26.10.2022
NO26.01.2023
GR27.01.2023
IS26.02.2023
PT27.02.2023
Former [2023/35]AT26.10.2022
CZ26.10.2022
DK26.10.2022
EE26.10.2022
ES26.10.2022
FI26.10.2022
HR26.10.2022
LT26.10.2022
LV26.10.2022
NL26.10.2022
PL26.10.2022
RO26.10.2022
RS26.10.2022
SE26.10.2022
SM26.10.2022
NO26.01.2023
GR27.01.2023
IS26.02.2023
PT27.02.2023
Former [2023/34]AT26.10.2022
DK26.10.2022
EE26.10.2022
ES26.10.2022
FI26.10.2022
HR26.10.2022
LT26.10.2022
LV26.10.2022
NL26.10.2022
PL26.10.2022
RS26.10.2022
SE26.10.2022
SM26.10.2022
NO26.01.2023
GR27.01.2023
IS26.02.2023
PT27.02.2023
Former [2023/33]AT26.10.2022
DK26.10.2022
ES26.10.2022
FI26.10.2022
HR26.10.2022
LT26.10.2022
LV26.10.2022
NL26.10.2022
PL26.10.2022
RS26.10.2022
SE26.10.2022
SM26.10.2022
NO26.01.2023
GR27.01.2023
IS26.02.2023
PT27.02.2023
Former [2023/25]AT26.10.2022
ES26.10.2022
FI26.10.2022
HR26.10.2022
LT26.10.2022
LV26.10.2022
NL26.10.2022
PL26.10.2022
RS26.10.2022
SE26.10.2022
NO26.01.2023
GR27.01.2023
IS26.02.2023
PT27.02.2023
Former [2023/23]AT26.10.2022
ES26.10.2022
FI26.10.2022
LT26.10.2022
LV26.10.2022
NL26.10.2022
SE26.10.2022
NO26.01.2023
GR27.01.2023
PT27.02.2023
Former [2023/22]AT26.10.2022
ES26.10.2022
FI26.10.2022
LT26.10.2022
NL26.10.2022
SE26.10.2022
NO26.01.2023
PT27.02.2023
Former [2023/20]LT26.10.2022
NL26.10.2022
NO26.01.2023
Former [2023/17]NL26.10.2022
Documents cited:Search[A]WO2008097887  (UNIV EMORY [US], et al) [A] 1-15 * figure 2; example 1 *;
 [A]WO2010127020  (JS GENETICS INC [US], et al) [A] 1-15* figure 1A; claim 1 *;
 [A]WO2016028887  (PACIFIC BIOSCIENCES CALIFORNIA [US]) [A] 1-15 * paragraphs [0100] , [ 129] *
International search[X]WO2014113204  (PERSONALIS INC [US]) [X] 1-16* see claims 6, 8, 26, 34, 47, 54 and 60, description, paragraphs [0083], [0132], [0154] and [0165] *
by applicantWO2008097887
 WO2010127020
 WO2016028887
    - JIA QWU HZHOU X et al., "A ''GC-rich'' method for mammalian gene expression: a dominant role of non-coding DNA GC content in regulation of mammalian gene expression", Sci China Life Sci., (20100000), vol. 53, no. 1, doi:10.1007/s11427-010-0003-x, pages 94 - 100, XP035977679

DOI:   http://dx.doi.org/10.1007/s11427-010-0003-x
    - HUANG WXIA QLUO S et al., "Distribution of fragile X mental retardation 1 CGG repeat and flanking haplotypes in a large Chinese population", Mol Genet Genomic Med., (20150000), vol. 3, no. 3, pages 172 - 181
    - CHEN XWANG JXIE H et al., "Fragile X syndrome screening in Chinese children with unknown intellectual developmental disorder", BMC Pediatr., (20150000), vol. 15, doi:10.1186/s12887-015-0394-8, page 77, XP021225928

DOI:   http://dx.doi.org/10.1186/s12887-015-0394-8
    - KIELECZAWA J., "Fundamentals of sequencing of difficult templates---an overview", J Biomol Tech., (20060000), vol. 17, no. 3, pages 207 - 217
    - HUBE FREVERDIAU PIOCHMANN SGRUEL Y, "Improved PCR method for amplification of GC-rich DNA sequences", Mol Biotechnol., (20050000), vol. 31, no. 1, doi:10.1385/MB:31:1:081, pages 81 - 84, XP002684995

DOI:   http://dx.doi.org/10.1385/MB:31:1:081
    - LI LYLI QYU YH et al., "A primer design strategy for PCR amplification of GC-rich DNA sequences", Clin Biochem., (20110000), vol. 44, no. 8-9, doi:10.1016/j.clinbiochem.2011.02.001, pages 692 - 698, XP028209462

DOI:   http://dx.doi.org/10.1016/j.clinbiochem.2011.02.001
    - JENSEN MAFUKUSHIMA MDAVIS RW, "DMSO and betaine greatly improve amplification of GC-rich constructs in de novo synthesis", PLoS One, (20100000), vol. 5, no. 6, doi:10.1371/journal.pone.0011024, page e11024, XP055265247

DOI:   http://dx.doi.org/10.1371/journal.pone.0011024
    - STRIEN JSANFT JMALL G, "Enhancement of PCR amplification of moderate GC-containing and highly GC-rich DNA sequences", Mol Biotechnol., (20130000), vol. 54, no. 3, pages 1048 - 1054
    - LOOMIS EW, EID JS, PELUSO P, ET AL., "Sequencing the unsequenceable: expanded CGG-repeat alleles of the fragile X gene.", Genome Res., (20130000), vol. 23, no. 1, doi:10.1101/gr.141705.112, pages 121 - 128, XP055266158

DOI:   http://dx.doi.org/10.1101/gr.141705.112
    - SCHADT EETURNER SKASARSKIS A, "A window into third-generation sequencing", Hum. Mol. Genet., (20100000), vol. 19, no. R2, doi:10.1093/hmg/ddq416, pages R227 - R240, XP055223002

DOI:   http://dx.doi.org/10.1093/hmg/ddq416
    - PHAM TTYIN JEID JS et al., "Single-locus enrichment without amplification for sequencing and direct detection of epigenetic modifications", Mol Genet Genomics, (20160000), vol. 291, no. 3, doi:10.1007/s00438-016-1167-2, pages 1491 - 1504, XP035882129

DOI:   http://dx.doi.org/10.1007/s00438-016-1167-2
    - "Target Enrichment via DNA Hybridization Capture. In: Shapiro B.", HORN S, Methods in Molecular Biology (Methods and Protocols), Humana Press, (20120000), vol. 840
    - DEAN FBHOSONO SFANG L et al., "Comprehensive human genome amplification using multiple displacement amplification", Proc Natl Acad Sci USA., (20020000), vol. 99, no. 8, doi:10.1073/pnas.082089499, pages 5261 - 5266, XP002297504

DOI:   http://dx.doi.org/10.1073/pnas.082089499
 CN20108032511
The EPO accepts no responsibility for the accuracy of data originating from other authorities; in particular, it does not guarantee that it is complete, up to date or fit for specific purposes.