blank Quick help
blank Maintenance news

Scheduled maintenance

Regular maintenance outages:
between 05.00 and 05.15 hrs CET (Monday to Sunday).

Other outages
Availability
Register Forum

2022.02.11

More...
blank News flashes

News Flashes

New version of the European Patent Register – SPC proceedings information in the Unitary Patent Register.

2024-07-24

More...
blank Related links

Extract from the Register of European Patents

EP About this file: EP3699293

EP3699293 - ENGINEERED TRANSCRIPTION ACTIVATOR-LIKE EFFECTOR (TALE) DOMAINS AND USES THEREOF [Right-click to bookmark this link]
StatusExamination is in progress
Status updated on  16.07.2021
Database last updated on 26.07.2024
FormerRequest for examination was made
Status updated on  05.03.2021
FormerThe application has been published
Status updated on  24.07.2020
Most recent event   Tooltip28.08.2023New entry: Renewal fee paid 
Applicant(s)For all designated states
President And Fellows Of Harvard College
17 Quincy Street
Cambridge, Massachusetts 02138 / US
[2020/35]
Inventor(s)01 / LIU, David R.
3 Whitman Circle
Lexington, MA 02040 / US
02 / GUILINGER, John Paul
340 Bear Club Drive
Ridgway, CO 81432 / US
03 / PATTANAYAK, Vikram
123 Holden Green
Cambridge, MA 02138 / US
 [2020/35]
Representative(s)Atkins, James Gordon John, et al
Kilburn & Strode LLP
Lacon London
84 Theobalds Road
London WC1X 8NL / GB
[2020/35]
Application number, filing date20152716.522.08.2014
[2020/35]
Priority number, dateUS201361868846P22.08.2013         Original published format: US 201361868846 P
US20141432051930.06.2014         Original published format: US201414320519
[2020/35]
Filing languageEN
Procedural languageEN
PublicationType: A1 Application with search report 
No.:EP3699293
Date:26.08.2020
Language:EN
[2020/35]
Search report(s)(Supplementary) European search report - dispatched on:EP13.05.2020
ClassificationIPC:C12Q1/68
[2020/35]
CPC:
C12N9/22 (EP,US); C12N15/01 (US); A61P31/18 (EP);
A61P35/00 (EP); A61P43/00 (EP); C12Q1/68 (EP,US);
C07K2319/00 (EP,US); C07K2319/80 (EP,US); C12Y301/00 (EP,US) (-)
Designated contracting statesAL,   AT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   LV,   MC,   MK,   MT,   NL,   NO,   PL,   PT,   RO,   RS,   SE,   SI,   SK,   SM,   TR [2021/14]
Former [2020/35]AL,  AT,  BE,  BG,  CH,  CY,  CZ,  DE,  DK,  EE,  ES,  FI,  FR,  GB,  GR,  HR,  HU,  IE,  IS,  IT,  LI,  LT,  LU,  LV,  MC,  MK,  MT,  NL,  NO,  PL,  PT,  RO,  RS,  SE,  SI,  SK,  SM,  TR 
TitleGerman:MANIPULIERTE TRANSKRIPTIONS-AKTIVATOR-ÄHNLICHE EFFEKTOR-DOMÄNEN UND VERWENDUNGEN DAVON[2020/35]
English:ENGINEERED TRANSCRIPTION ACTIVATOR-LIKE EFFECTOR (TALE) DOMAINS AND USES THEREOF[2020/35]
French:DOMAINES D'EFFECTEUR DE TYPE ACTIVATEUR DE TRANSCRIPTION (TALE) ET LEURS UTILISATIONS[2020/35]
Examination procedure26.02.2021Amendment by applicant (claims and/or description)
26.02.2021Examination requested  [2021/14]
26.02.2021Date on which the examining division has become responsible
19.07.2021Despatch of a communication from the examining division (Time limit: M04)
29.11.2021Reply to a communication from the examining division
10.08.2022Despatch of a communication from the examining division (Time limit: M06)
20.02.2023Reply to a communication from the examining division
Parent application(s)   TooltipEP14761757.5  / EP3036342
Fees paidRenewal fee
20.01.2020Renewal fee patent year 03
20.01.2020Renewal fee patent year 04
20.01.2020Renewal fee patent year 05
20.01.2020Renewal fee patent year 06
27.08.2020Renewal fee patent year 07
27.08.2021Renewal fee patent year 08
29.08.2022Renewal fee patent year 09
28.08.2023Renewal fee patent year 10
Opt-out from the exclusive  Tooltip
competence of the Unified
Patent Court
See the Register of the Unified Patent Court for opt-out data
Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Documents cited:Search[I]WO2011146121  (SANGAMO BIOSCIENCES INC [US], et al) [I] 1-5,13-15 * Examples and claims and SEQ ID NO:363 *;
 [X]WO2012138927  (DUCHATEAU PHILIPPE [FR], et al) [X] 1,5,7,8,13-15 * Page 15, Examples 3, 3a, Figure 6. *;
    [ ] - "Xanthomonas sp. AvrBs3 C-terminal domain sequence, SEQ ID 205.", Geneseq, (20121206), Database accession no. BAD77058, URL: EBI [ ] * sequence . *
 [XI]  - JEFFREY C MILLER ET AL, "A TALE nuclease architecture for efficient genome editing", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, UNITED STATES, vol. 29, no. 2, doi:10.1038/NBT.1755 ?, ISSN 1546-1696, (20110201), pages 143 - 150, (20101222), XP002685898 [X] 1,5,13-15 * Figures 1- 3, Discussion , Supplementary Figure 1 * [I] 7,12

DOI:   http://dx.doi.org/10.1038/nbt.1755
 [A]  - CLAUDIO MUSSOLINO ET AL, "TALE nucleases: tailored genome engineering made easy", CURRENT OPINION IN BIOTECHNOLOGY, (20121001), vol. 23, no. 5, doi:10.1016/j.copbio.2012.01.013, ISSN 0958-1669, pages 644 - 650, XP055071668 [A] 1-15 * page 647 *

DOI:   http://dx.doi.org/10.1016/j.copbio.2012.01.013
 [XP]  - JOHN P GUILINGER ET AL, "Broad specificity profiling of TALENs results in engineered nucleases with improved DNA-cleavage specificity", NATURE METHODS, (20140216), vol. 11, no. 4, doi:10.1038/nmeth.2845, ISSN 1548-7091, pages 429 - 435, XP055148794 [XP] 1-15 * the whole document *

DOI:   http://dx.doi.org/10.1038/nmeth.2845
by applicantWO2013066438
    - MILLER, JEFFREY, "A TALE nuclease architecture for efficient genome editing", Nature Biotechnology, (20110200), vol. 29, no. 2, doi:10.1038/nbt.1755, pages 143 - 8, XP055568321

DOI:   http://dx.doi.org/10.1038/nbt.1755
    - ZHANG, FENG, "Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription", Nature Biotechnology, (20110200), vol. 29, no. 2, doi:10.1038/nbt.1775, pages 149 - 53, XP055005146

DOI:   http://dx.doi.org/10.1038/nbt.1775
    - "Transcriptional Activators of Human Genes with Programmable DNA-Specificity", GEIPLER, R.SCHOLZE, H.HAHN, S.STREUBEL, J.BONAS, U.BEHRENS, S. E.BOCH, J., PLoS ONE, (20110000), vol. 6, page e19722
    - BOCH, JENS, "TALEs of genome targeting", Nature Biotechnology, (20110200), vol. 29, no. 2, doi:10.1038/nbt.1767, pages 135 - 6, XP055077654

DOI:   http://dx.doi.org/10.1038/nbt.1767
    - BOCH, JENS, "Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors", Science, (20091200), vol. 326, no. 5959, pages 1509 - 12
    - MOSCOU, MATTHEW J.ADAM J. BOGDANOVE, "A Simple Cipher Governs DNA Recognition by TAL Effectors", Science, (20091200), vol. 326, no. 5959, page 1501, XP002599998
    - CHRISTIAN, MICHELLE, "Targeting DNA Double-Strand Breaks with TAL Effector Nucleases", Genetics, (20101000), vol. 186, no. 2, doi:10.1534/GENETICS.110.120717, pages 757 - 61, XP002632806

DOI:   http://dx.doi.org/10.1534/GENETICS.110.120717
    - LI, TING, "TAL nucleases (TALNs): hybrid proteins composed of TAL effectors and Fokl DNA-cleavage domain", Nucleic Acids Research, (20100800), vol. 39, pages 1 - 14
    - MAHFOUZ, MAGDY M., "De novo-engineered transcription activator-like effector (TALE) hybrid nuclease with novel DNA binding specificity creates double-strand breaks", PNAS, (20100200), vol. 108, no. 6, doi:10.1073/pnas.1019533108, pages 2623 - 8, XP055007615

DOI:   http://dx.doi.org/10.1073/pnas.1019533108
    - HOCKEMEYER, D.WANG, H.KIANI, S.LAI, C. S.GAO, Q.CASSADY, J. P.COST, G. J.ZHANG, L. et al., "Genetic engineering of human pluripotent cells using TALE nucleases", Nature Biotechnology, (20110000), vol. 29, no. 8, doi:10.1038/nbt.1927, XP055018244

DOI:   http://dx.doi.org/10.1038/nbt.1927
    - WOOD, A. J.LO, T. -W.ZEITLER, B.PICKLE, C. S.RALSTON, E. J.LEE, A. H.AMORA, R.MILLER, J. C. et al., "Targeted Genome Editing Across Species Using ZFNs and TALENs", Science, (20110000), vol. 333, page 6040
    - TESSON, L.USAL, C.MENORET, S. V.LEUNG, E.NILES, B. J.REMY, S. V.SANTIAGO, Y.VINCENT, A. I. et al., "Knockout rats generated by embryo microinjection of TALENs", Nature Biotechnology, (20110000), vol. 29, no. 8, doi:10.1038/NBT.1940, page 695, XP002681970

DOI:   http://dx.doi.org/10.1038/NBT.1940
    - HUANG, P.XIAO, A.ZHOU, M.ZHU, Z.LIN, S.ZHANG, B., "Heritable gene targeting in zebrafish using customized TALENs", Nature Biotechnology, (20110000), vol. 29, no. 8, page 699
    - DOYON, Y.VO, T. D.MENDEL, M. C.GREENBERG, S. G.WANG, J.XIA, D. F.MILLER, J. C.URNOV, F. D. et al., "Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures", Nature Methods, (20100000), vol. 8, no. 1, doi:10.1038/nmeth.1539, pages 74 - 79, XP055075068

DOI:   http://dx.doi.org/10.1038/nmeth.1539
    - SZCZEPEK, M.BRONDANI, V.BUCHEL, J.SERRANO, L.SEGAL, D. J.CATHOMEN, T., "Structure-based redesign of the dimerization interface reduces the toxicity of zinc-finger nucleases", Nature Biotechnology, (20070000), vol. 25, no. 7, doi:10.1038/nbt1317, page 786, XP002465118

DOI:   http://dx.doi.org/10.1038/nbt1317
    - GUO, J.GAJ, T.BARBAS III, C. F., "Directed Evolution of an Enhanced and Highly Efficient Fokl Cleavage Domain for Zinc Finger Nucleases", Journal of Molecular Biology, (20100000), vol. 400, no. 1, doi:10.1016/j.jmb.2010.04.060, page 96, XP055491626

DOI:   http://dx.doi.org/10.1016/j.jmb.2010.04.060
    - SANDER, "Targeted gene disruption in somatic zebrafish cells using engineered TALENs", Nature Biotechnology, (20110805), vol. 29, doi:10.1038/nbt.1934, pages 697 - 98, XP055263701

DOI:   http://dx.doi.org/10.1038/nbt.1934
    - SANDER, J. D.CADE, L.KHAYTER, C.REYON, D.PETERSON, R. T.JOUNG, J. K.YEH, J. R. J., "Targeted gene disruption in somatic zebrafish cells using engineered TALENs", Nature Biotechnology, (20110000), vol. 29, no. 8, doi:10.1038/nbt.1934, page 697, XP055263701

DOI:   http://dx.doi.org/10.1038/nbt.1934
    - MOSCOU, M.J.BOGDANOVE, A.J., "A simple cipher governs DNA recognition by TAL effectors", Science, (20090000), vol. 326, page 1501, XP002599998
    - BOCH, J. et al., "Breaking the code of DNA binding specificity of TAL-type III effectors", Science, (20090000), vol. 326, doi:10.1126/science.1178811, pages 1509 - 1512, XP055250971

DOI:   http://dx.doi.org/10.1126/science.1178811
    - DOYON, Y. et al., "Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures", Nat Methods, (20110000), vol. 8, doi:10.1038/nmeth.1539, pages 74 - 79, XP055075068

DOI:   http://dx.doi.org/10.1038/nmeth.1539
    - CADE, L. et al., "Highly efficient generation of heritable zebrafish gene mutations using homo- and heterodimeric TALENs", Nucleic Acids Res, (20120000), vol. 40, doi:10.1093/nar/gks518, pages 8001 - 8010, XP055086692

DOI:   http://dx.doi.org/10.1093/nar/gks518
    - MILLER, J.C. et al., "A TALE nuclease architecture for efficient genome editing", Nat Biotechnol, (20110000), vol. 29, doi:10.1038/nbt.1755, pages 143 - 148, XP055568321

DOI:   http://dx.doi.org/10.1038/nbt.1755
    - BEDELL, V.M. et al., "In vivo genome editing using a high-efficiency TALEN system", Nature, (20120000), vol. 491, doi:10.1038/nature11537, pages 114 - 118, XP055048281

DOI:   http://dx.doi.org/10.1038/nature11537
    - HOCKEMEYER, D. et al., "Genetic engineering of human pluripotent cells using TALE nucleases", Nat Biotechnol, (20110000), vol. 29, doi:10.1038/nbt.1927, pages 731 - 734, XP055018244

DOI:   http://dx.doi.org/10.1038/nbt.1927
    - CERMAK, T. et al., "Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting", Nucleic Acids Res, (20110000), vol. 39, page e82
    - TESSON, L. et al., "Knockout rats generated by embryo microinjection of TALENs", Nat Biotechnol, (20110000), vol. 29, pages 695 - 696
    - MOORE, F.E. et al., "Improved somatic mutagenesis in zebrafish using transcription activator-like effector nucleases (TALENs", PLoS One, (20120000), vol. 7, doi:10.1371/journal.pone.0037877, page e37877, XP055086697

DOI:   http://dx.doi.org/10.1371/journal.pone.0037877
    - WOOD, A.J. et al., "Targeted genome editing across species using ZFNs and TALENs", Science, (20110000), vol. 333, doi:10.1126/science.1207773, page 307, XP055102329

DOI:   http://dx.doi.org/10.1126/science.1207773
    - REYON, D. et al., "FLASH assembly of TALENs for high-throughput genome editing", Nat Biotechnol, (20120000), vol. 30, doi:10.1038/nbt.2170, pages 460 - 465, XP055171172

DOI:   http://dx.doi.org/10.1038/nbt.2170
    - MUSSOLINO, C. et al., "A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity", Nucleic Acids Res, (20110000), vol. 39, doi:10.1093/nar/gkr597, pages 9283 - 9293, XP055021128

DOI:   http://dx.doi.org/10.1093/nar/gkr597
    - PATTANAYAK, V.RAMIREZ, C.L.JOUNG, J.K.LIU, D.R., "Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection", Nat Methods, (20110000), vol. 8, doi:10.1038/nmeth.1670, pages 765 - 770, XP055650557

DOI:   http://dx.doi.org/10.1038/nmeth.1670
    - LI, T. et al., "Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes", Nucleic Acids Res, (20110000), vol. 39, doi:10.1093/NAR/GKR188, pages 6315 - 6325, XP002681969

DOI:   http://dx.doi.org/10.1093/NAR/GKR188
    - LEI, Y. et al., "Efficient targeted gene disruption in Xenopus embryos using engineered transcription activator-like effector nucleases (TALENs", Proc Natl Acad Sci U S A, (20120000), vol. 109, doi:10.1073/pnas.1215421109, pages 17484 - 17489, XP055302289

DOI:   http://dx.doi.org/10.1073/pnas.1215421109
    - KIM, Y. et al., "A library of TAL effector nucleases spanning the human genome", Nat Biotechnol, (20130000), vol. 31, doi:10.1038/nbt.2517, pages 251 - 258, XP055115496

DOI:   http://dx.doi.org/10.1038/nbt.2517
    - DAHLEM, T.J. et al., "Simple methods for generating and detecting locus-specific mutations induced with TALENs in the zebrafish genome", PLoS Genet, (20120000), vol. 8, page el002861
    - MAUL, G.G.DEAVEN, L., "Quantitative determination of nuclear pore complexes in cycling cells with differing DNA content", J Cell Biol, (19770000), vol. 73, pages 748 - 760
    - HUANG, B. et al., "Counting low-copy number proteins in a single cell", Science, (20070000), vol. 315, doi:10.1126/science.1133992, pages 81 - 84, XP055346999

DOI:   http://dx.doi.org/10.1126/science.1133992
    - BECK, M. et al., "The quantitative proteome of a human cell line", Mol Syst Biol, (20110000), vol. 7, page 549
    - CHRISTIAN, M.L. et al., "Targeting G with TAL effectors: a comparison of activities of TALENs constructed with NN and NK repeat variable di-residues", PLoS One, (20120000), vol. 7, page e45383
    - YAN, T. et al., "PatMatch: a program for finding patterns in peptide and nucleotide sequences", Nucleic Acids Res, (20050000), vol. 33, pages W262 - 266
    - KIM, Y.KWEON, J.KIM, J.S., "TALENs and ZFNs are associated with different mutation signatures", Nat Methods, (20130000), vol. 10, doi:10.1038/nmeth.2364, page 185, XP055077422

DOI:   http://dx.doi.org/10.1038/nmeth.2364
    - MCNAUGHTON, B.R.CRONICAN, J.J.THOMPSON, D.B.LIU, D.R., "Mammalian cell penetration, siRNA transfection, and DNA transfection by supercharged proteins", Proc Natl Acad Sci U S A, (20090000), vol. 106, pages 6111 - 6116
    - SUN, N.LIANG, J.ABIL, Z.ZHAO, H., "Optimized TAL effector nucleases (TALENs) for use in treatment of sickle cell disease", Mol Biosyst, (20120000), vol. 8, pages 1255 - 1263
    - CONG, L.ZHOU, R.KUO, Y.C.CUNNIFF, M.ZHANG, F., "Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains", Nat Commun, (20120000), vol. 3, doi:10.1038/ncomms1962, page 968, XP002700145

DOI:   http://dx.doi.org/10.1038/ncomms1962
    - GABRIEL, R. et al., "An unbiased genome-wide analysis of zinc-finger nuclease specificity", Nat Biotechnol, (20110000), vol. 29, doi:10.1038/nbt.1948, pages 816 - 823, XP055073828

DOI:   http://dx.doi.org/10.1038/nbt.1948
    - GAO, H.WU, X.CHAI, J.HAN, Z., "Crystal structure of a TALE protein reveals an extended N-terminal DNA binding region", Cell Res, (20120000), vol. 22, pages 1716 - 1720
    - MAHFOUZ, M.M. et al., "De novo-engineered transcription activator-like effector (TALE) hybrid nuclease with novel DNA binding specificity creates double-strand breaks", Proc Natl Acad Sci U S A, (20110000), vol. 108, doi:10.1073/pnas.1019533108, pages 2623 - 2628, XP055007615

DOI:   http://dx.doi.org/10.1073/pnas.1019533108
    - LARKIN, M.A. et al., "Clustal W and Clustal X version 2.0", Bioinformatics, (20070000), vol. 23, pages 2947 - 2948
The EPO accepts no responsibility for the accuracy of data originating from other authorities; in particular, it does not guarantee that it is complete, up to date or fit for specific purposes.