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Extract from the Register of European Patents

EP About this file: EP3995584

EP3995584 - GENE SILENCING [Right-click to bookmark this link]
StatusExamination is in progress
Status updated on  07.09.2023
Database last updated on 11.04.2026
FormerRequest for examination was made
Status updated on  18.11.2022
FormerThe application has been published
Status updated on  08.04.2022
Most recent event   Tooltip14.03.2026New entry: Observations by third parties 
Applicant(s)For all designated states
Ospedale San Raffaele S.r.l.
Via Olgettina 60
20132 Milano / IT
For all designated states
Fondazione Telethon
Via Varese 16B
00185 Roma / IT
[2022/19]
Inventor(s)01 / NALDINI, Luigi
20132 Milano / IT
02 / LOMBARDO, Angelo Leone
20132 Milano / IT
03 / AMABILE, Angelo
20132 Milano / IT
04 / MIGLIARA, Alessandro
20132 Milano / IT
 [2022/19]
Representative(s)Hoffmann Eitle
Patent- und Rechtsanwälte PartmbB
Arabellastraße 30
81925 München / DE
[N/P]
Former [2022/19]D Young & Co LLP
120 Holborn
London EC1N 2DY / GB
Application number, filing date21197502.423.10.2015
[2022/19]
Priority number, dateGB2014001896524.10.2014         Original published format: GB 201418965
[2022/19]
Filing languageEN
Procedural languageEN
PublicationType: A1 Application with search report 
No.:EP3995584
Date:11.05.2022
Language:EN
[2022/19]
Search report(s)(Supplementary) European search report - dispatched on:EP04.04.2022
ClassificationIPC:C12N15/85, A61K48/00, A61K38/45, // C12N9/10
[2022/19]
CPC:
A61K38/45 (EP,CN,US); C12N9/1007 (EP,CN,US); C12N15/113 (US);
A61K35/12 (EP,CN,US); A61K38/1709 (EP,CN,US); A61K48/005 (EP,CN,US);
C07K14/4703 (EP,US); C12N15/85 (CN); C12N9/22 (EP,US);
C12Y201/01037 (EP,US); C12Y201/01043 (EP,US); A61K38/00 (EP,US);
C07K2319/80 (EP,US); C12N2310/20 (US); C12N2740/16043 (EP,US);
C12N2800/80 (US) (-)
Designated contracting statesAL,   AT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   LV,   MC,   MK,   MT,   NL,   NO,   PL,   PT,   RO,   RS,   SE,   SI,   SK,   SM,   TR [2022/51]
Former [2022/19]AL,  AT,  BE,  BG,  CH,  CY,  CZ,  DE,  DK,  EE,  ES,  FI,  FR,  GB,  GR,  HR,  HU,  IE,  IS,  IT,  LI,  LT,  LU,  LV,  MC,  MK,  MT,  NL,  NO,  PL,  PT,  RO,  RS,  SE,  SI,  SK,  SM,  TR 
TitleGerman:PERMANENTES GEN-SILENCING[2022/19]
English:GENE SILENCING[2022/19]
French:EXTINCTION DE GÈNE PERMANENTE[2022/19]
Examination procedure11.11.2022Examination requested  [2022/51]
11.11.2022Date on which the examining division has become responsible
02.02.2023Amendment by applicant (claims and/or description)
06.09.2023Despatch of a communication from the examining division (Time limit: M06)
10.04.2024Despatch of communication that the application is deemed to be withdrawn, reason: reply to the communication from the examining division not received in time
10.06.2024Reply to a communication from the examining division
13.02.2025Observations by third parties
27.02.2025Despatch of a communication from the examining division (Time limit: M04)
27.06.2025Reply to a communication from the examining division
07.08.2025Observations by third parties
16.10.2025Despatch of a communication from the examining division (Time limit: M04)
16.02.2026Reply to a communication from the examining division
13.03.2026Observations by third parties
Parent application(s)   TooltipEP15794644.3  / EP3209783
Request for further processing for:The application is deemed to be withdrawn due to failure to reply to the examination report
10.06.2024Request for further processing filed
10.06.2024Full payment received (date of receipt of payment)
Request granted
18.06.2024Decision despatched
The application is deemed to be withdrawn due to failure to reply to the Extended European Search Report/Written Opinion of the International Searching Authority/International Preliminary Examination Report/Supplementary international search report/Supplementary European search report
02.02.2023Request for further processing filed
02.02.2023Full payment received (date of receipt of payment)
Request granted
08.02.2023Decision despatched
Fees paidRenewal fee
17.01.2022Renewal fee patent year 03
17.01.2022Renewal fee patent year 04
17.01.2022Renewal fee patent year 05
17.01.2022Renewal fee patent year 06
17.01.2022Renewal fee patent year 07
20.10.2022Renewal fee patent year 08
27.10.2023Renewal fee patent year 09
28.10.2024Renewal fee patent year 10
27.10.2025Renewal fee patent year 11
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Documents cited:Search[A] WO03072788  (WISTAR INST et al.)
 [A]   LI HONGWEI ET AL: "The histone methyltransferase SETDB1 and the DNA methyltransferase DNMT3A interact directly and localize to promoters silenced in cancer cells", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, US, vol. 281, no. 28, 14 July 2006 (2006-07-14), pages 19489 - 19500, XP002544945, ISSN: 0021-9258, DOI: 10.1074/JBC.M513249200

DOI:   http://dx.doi.org/10.1074/JBC.M513249200
 [A]   K. AYYANATHAN ET AL: "Regulated recruitment of HP1 to a euchromatic gene induces mitotically heritable, epigenetic gene silencing: a mammalian cell culture model of gene variegation", GENES AND DEVELOPMENT., vol. 17, no. 15, 17 July 2003 (2003-07-17), US, pages 1855 - 1869, XP055245885, ISSN: 0890-9369, DOI: 10.1101/gad.1102803

DOI:   http://dx.doi.org/10.1101/gad.1102803
 [A]   XINGHUI ZHAO ET AL: "Intracellular delivery of artificial transcription factors fused to the protein transduction domain of HIV-1 Tat", PROTEIN EXPRESSION AND PURIFICATION, vol. 90, no. 1, 1 July 2013 (2013-07-01), pages 27 - 33, XP055096285, ISSN: 1046-5928, DOI: 10.1016/j.pep.2013.04.007

DOI:   http://dx.doi.org/10.1016/j.pep.2013.04.007
 [A]   BROUDE EUGENIA V ET AL: "P21 (CDKN1A) is a Negative Regulator of p53 stability", CELL CYCLE, LANDES BIOSCIENCE, vol. 6, no. 12, 1 June 2007 (2007-06-01), pages 1468 - 1471, XP009152430, ISSN: 1538-4101
 [A]   KUEHNEL F ET AL: "Tumor-specific adenoviral gene therapy: Transcriptional repression of gene expression by utilizing p53-signal transduction pathways", CANCER GENE THERAPY, APPLETON & LANGE, GB, vol. 11, no. 1, 1 January 2004 (2004-01-01), pages 28 - 40, XP002372745, ISSN: 0929-1903, DOI: 10.1038/SJ.CGT.7700632 [A] 1-17 * page 19495, paragraph 2 *

DOI:   http://dx.doi.org/10.1038/sj.cgt.7700632
 [A]   F. LI ET AL: "Chimeric DNA methyltransferases target DNA methylation to specific DNA sequences and repress expression of target genes", NUCLEIC ACIDS RESEARCH, vol. 35, no. 1, 7 December 2006 (2006-12-07), GB, pages 100 - 112, XP055246015, ISSN: 0305-1048, DOI: 10.1093/nar/gkl1035

DOI:   http://dx.doi.org/10.1093/nar/gkl1035
 [A]   KIM ET AL: "Zinc-fingers and homeoboxes 1 (ZHX1) binds DNA methyltransferase (DNMT) 3B to enhance DNMT3B-mediated transcriptional repression", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 355, no. 2, 24 February 2007 (2007-02-24), pages 318 - 323, XP005899650, ISSN: 0006-291X, DOI: 10.1016/J.BBRC.2007.01.187 [A] 1-17 * abstract *

DOI:   http://dx.doi.org/10.1016/j.bbrc.2007.01.187
 [A]   ALBERT JELTSCH ET AL: "Application of DNA methyltransferases in targeted DNA methylation", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 75, no. 6, 13 April 2007 (2007-04-13), pages 1233 - 1240, XP019513780, ISSN: 1432-0614, DOI: 10.1007/S00253-007-0966-0 [A] 1-17 * abstract *

DOI:   http://dx.doi.org/10.1007/s00253-007-0966-0
 [A]   RAUL URRUTIA: "KRAB-containing zinc-finger repressor proteins", GENOME BIOLOGY, vol. 4, no. 10, 23 September 2003 (2003-09-23), GB, pages 231, XP055245881, ISSN: 1465-6906, DOI: 10.1186/gb-2003-4-10-231 [A] 1-17 * page 1469, paragraph "Results" *

DOI:   http://dx.doi.org/10.1186/gb-2003-4-10-231
 [A]   A AMABILE ET AL: "Permanent Epigenetic Silencing of Human Genes With Artificial Transcriptional Repressors", 18. ASGCT ANNUAL MEETING, 16 May 2015 (2015-05-16), XP055245901 [A] 1-17 * abstract *

DOI:   http://dx.doi.org/10.1016/S1525-0016(16)34299-X
by applicantWO9805635
 WO9807859
 WO9809985
other  ABU NASAR SIDDIQUE, SUNEETHA NUNNA, ARUMUGAM RAJAVELU, YINGYING ZHANG, RENATA Z. JURKOWSKA, RICHARD REINHARDT, MARIANNE G. ROTS, S: "Targeted Methylation and Gene Silencing of VEGF-A in Human Cells by Using a Designed Dnmt3a–Dnmt3L Single-Chain Fusion Protein with Increased DNA Methylation Activity", JOURNAL OF MOLECULAR BIOLOGY, vol. 425, no. 3, 1 February 2013 (2013-02-01), United Kingdom , pages 479 - 491, XP055623261, ISSN: 0022-2836, DOI: 10.1016/j.jmb.2012.11.038

DOI:   http://dx.doi.org/10.1016/j.jmb.2012.11.038
   MOUSSA HAGAR F.; ANGSTMAN JAMES F.; KHALIL AHMAD S.: "Here to stay: Writing lasting epigenetic memories", CELL, vol. 184, no. 9, 29 April 2021 (2021-04-29), Amsterdam NL , pages 2281 - 2283, XP086555042, ISSN: 0092-8674, DOI: 10.1016/j.cell.2021.04.007

DOI:   http://dx.doi.org/10.1016/j.cell.2021.04.007
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