blank Quick help
blank Maintenance news

Scheduled maintenance

Regular maintenance outages:
between 05.00 and 05.15 hrs CET (Monday to Sunday).

Other outages
Availability
Register Forum

2022.02.11

More...
blank News flashes

News flashes

New version of the European Patent Register - SPC information for Unitary Patents.

2024-03-06

More...
blank Related links

Extract from the Register of European Patents

EP About this file: EP3995591

EP3995591 - NUCLEASE PROFILING SYSTEM [Right-click to bookmark this link]
StatusThe patent has been granted
Status updated on  08.12.2023
Database last updated on 08.06.2024
FormerGrant of patent is intended
Status updated on  26.07.2023
FormerRequest for examination was made
Status updated on  11.11.2022
FormerThe application has been published
Status updated on  08.04.2022
Most recent event   Tooltip08.12.2023(Expected) grantpublished on 10.01.2024  [2024/02]
Applicant(s)For all designated states
President And Fellows Of Harvard College
17 Quincy Street
Cambridge, Massachusetts 02138 / US
[2022/19]
Inventor(s)01 / LIU, David, R.
Lexington, MA 02420 / US
02 / PATTANAYAK, Vikram
Cambridge, MA 02138 / US
 [2022/19]
Representative(s)Atkins, James Gordon John, et al
Kilburn & Strode LLP
Lacon London
84 Theobalds Road
London WC1X 8NL / GB
[2022/19]
Application number, filing date21200987.208.08.2014
[2022/19]
Priority number, dateUS201361864289P09.08.2013         Original published format: US 201361864289 P
US20141432037030.06.2014         Original published format: US201414320370
US20141432041330.06.2014         Original published format: US201414320413
[2022/19]
Filing languageEN
Procedural languageEN
PublicationType: A1 Application with search report 
No.:EP3995591
Date:11.05.2022
Language:EN
[2022/19]
Type: B1 Patent specification 
No.:EP3995591
Date:10.01.2024
Language:EN
[2024/02]
Search report(s)(Supplementary) European search report - dispatched on:EP08.04.2022
ClassificationIPC:C12Q1/6816, C12Q1/6869
[2022/19]
CPC:
C12Q1/6816 (EP,US); C12Q1/44 (US); A61K38/465 (US);
A61P31/18 (EP); A61P35/00 (EP); C12N9/22 (US);
C12Q1/6869 (EP,US); C12Q1/6874 (US); C12Q1/6883 (US);
C12Y301/00 (EP,US); C12Q2600/156 (US) (-)
C-Set:
C12Q1/6816, C12Q2521/301, C12Q2522/101, C12Q2535/122, C12Q2563/131, C12Q2563/149 (EP,US);
C12Q1/6869, C12Q2521/301, C12Q2522/101, C12Q2535/122, C12Q2563/131, C12Q2563/149 (US,EP)
Designated contracting statesAL,   AT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   LV,   MC,   MK,   MT,   NL,   NO,   PL,   PT,   RO,   RS,   SE,   SI,   SK,   SM,   TR [2022/50]
Former [2022/19]AL,  AT,  BE,  BG,  CH,  CY,  CZ,  DE,  DK,  EE,  ES,  FI,  FR,  GB,  GR,  HR,  HU,  IE,  IS,  IT,  LI,  LT,  LU,  LV,  MC,  MK,  MT,  NL,  NO,  PL,  PT,  RO,  RS,  SE,  SI,  SK,  SM,  TR 
TitleGerman:NUKLEASEPROFILIERUNGSSYSTEM[2022/19]
English:NUCLEASE PROFILING SYSTEM[2022/19]
French:SYSTÈME DE PROFILAGE DE NUCLÉASE[2022/19]
Examination procedure10.11.2022Amendment by applicant (claims and/or description)
10.11.2022Examination requested  [2022/50]
10.11.2022Date on which the examining division has become responsible
27.07.2023Communication of intention to grant the patent
05.12.2023Fee for grant paid
05.12.2023Fee for publishing/printing paid
05.12.2023Receipt of the translation of the claim(s)
Parent application(s)   TooltipEP14755947.0  / EP3030650
EP18199195.1  / EP3486318
Fees paidRenewal fee
05.10.2021Renewal fee patent year 03
05.10.2021Renewal fee patent year 04
05.10.2021Renewal fee patent year 05
05.10.2021Renewal fee patent year 06
05.10.2021Renewal fee patent year 07
05.10.2021Renewal fee patent year 08
29.08.2022Renewal fee patent year 09
28.08.2023Renewal fee patent year 10
Opt-out from the exclusive  Tooltip
competence of the Unified
Patent Court
See the Register of the Unified Patent Court for opt-out data
Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Documents cited:Search[AD]WO2013066438  (HARVARD COLLEGE [US], et al) [AD] 1-20 * the whole document * * para. 10, 95, 96, 102-105, claims 27-38, 70-80 *;
 [A]  - PRASHANT MALI ET AL, "CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering", NATURE BIOTECHNOLOGY, (20130801), vol. 31, no. 9, doi:10.1038/nbt.2675, ISSN 1087-0156, pages 833 - 838, XP055149275 [A] 1-20 * the whole document *

DOI:   http://dx.doi.org/10.1038/nbt.2675
 [A]  - PATRICK D HSU ET AL, "DNA targeting specificity of RNA-guided Cas9 nucleases", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, UNITED STATES, vol. 31, no. 9, doi:10.1038/NBT.2647, ISSN 1546-1696, (20130721), pages 827 - 832, (20130721), XP002718604 [A] 1-20 * the whole document *

DOI:   http://dx.doi.org/10.1038/nbt.2647
 [IP]  - JOHN P GUILINGER ET AL, "Broad specificity profiling of TALENs results in engineered nucleases with improved DNA-cleavage specificity", NATURE METHODS, (20140216), vol. 11, no. 4, doi:10.1038/nmeth.2845, ISSN 1548-7091, pages 429 - 435, XP055148794 [IP] 1-20 * the whole document *

DOI:   http://dx.doi.org/10.1038/nmeth.2845
 [IP]  - VIKRAM PATTANAYAK ET AL, "High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity", NATURE BIOTECHNOLOGY, (20130811), vol. 31, no. 9, doi:10.1038/nbt.2673, ISSN 1087-0156, pages 839 - 843, XP055148795 [IP] 1-20 * the whole document *

DOI:   http://dx.doi.org/10.1038/nbt.2673
by applicantWO2013066438
    - PATTANAYAK, V.RAMIREZ, C.L.JOUNG, J.K.LIU, D.R., "Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection", Nature methods, (20110000), vol. 8, doi:10.1038/nmeth.1670, pages 765 - 770, XP055650557

DOI:   http://dx.doi.org/10.1038/nmeth.1670
    - JINEK M.CHYLINSKI K.FONFARA I.HAUER M.DOUDNA J.A.CHARPENTIER E., "A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity", Science, (20120000), vol. 337, doi:10.1126/science.1225829, pages 816 - 821, XP055229606

DOI:   http://dx.doi.org/10.1126/science.1225829
    - FERRETTI J.J.MCSHAN W.M.AJDIC D.J.SAVIC D.J.SAVIC G.LYON K.PRIMEAUX C.SEZATE S.SUVOROV A.N.KENTON S., "Complete genome sequence of an M1 strain of Streptococcus pyogenes", Proc. Natl. Acad. Sci. U.S.A., (20010000), vol. 98, doi:10.1073/pnas.071559398, pages 4658 - 4663, XP002344854

DOI:   http://dx.doi.org/10.1073/pnas.071559398
    - DELTCHEVA E.CHYLINSKI K.SHARMA C.M.GONZALES K.CHAO Y.PIRZADA Z.A.ECKERT M.R.VOGEL J.CHARPENTIER E., "CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III", Nature, (20110000), vol. 471, doi:10.1038/nature09886, pages 602 - 607, XP055308803

DOI:   http://dx.doi.org/10.1038/nature09886
    - CHYLINSKIRHUNCHARPENTIER, "The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems", RNA Biology, (20130000), vol. 10, no. 5, doi:10.4161/rna.24321, pages 726 - 737, XP055116068

DOI:   http://dx.doi.org/10.4161/rna.24321
    - K. C. NICOLAOUA. L. SMITHE. W. YUE, "Chemistry and biology of natural and designed enediynes", PNAS, (19930000), vol. 90, no. 13, pages 5881 - 5888
    - S. WALKERR. LANDOVITZW.D. DINGG.A. ELLESTADD. KAHNE, "Cleavage behavior of calicheamicin gamma 1 and calicheamicin T", Proc Natl Acad Sci U.S.A., (19920000), vol. 89, no. 10, pages 4608 - 12
    - ADRIAN L. SMITHK. C. BICOLAOU, "The Enediyne Antibiotics", J. Med. Chem., (19960000), vol. 39, no. 11, pages 2103 - 2117
    - EDGELL DR, "Selfish DNA: homing endonucleases find a home", Curr Biol, (20090200), vol. 19, no. 3, doi:10.1016/j.cub.2008.12.019, pages R115 - R117, XP025916026

DOI:   http://dx.doi.org/10.1016/j.cub.2008.12.019
    - JASIN M, "Genetic manipulation of genomes with rare-cutting endonucleases", Trends Genet, (19960600), vol. 12, no. 6, doi:10.1016/0168-9525(96)10019-6, pages 224 - 8, XP022519434

DOI:   http://dx.doi.org/10.1016/0168-9525(96)10019-6
    - BURT AKOUFOPANOU V, "Homing endonuclease genes: the rise and fall and rise again of a selfish element", Curr Opin Genet Dev, (20041200), vol. 14, no. 6, doi:10.1016/j.gde.2004.09.010, pages 609 - 15, XP004628734

DOI:   http://dx.doi.org/10.1016/j.gde.2004.09.010
    - CONG, L. et al., "Multiplex genome engineering using CRISPR/Cas systems", Science, (20130000), vol. 339, doi:10.1126/science.1231143, pages 819 - 823, XP055458249

DOI:   http://dx.doi.org/10.1126/science.1231143
    - MALI, P. et al., "RNA-guided human genome engineering via Cas9", Science, (20130000), vol. 339, doi:10.1126/science.1232033, pages 823 - 826, XP055469277

DOI:   http://dx.doi.org/10.1126/science.1232033
    - HWANG, W.Y. et al., "Efficient genome editing in zebrafish using a CRISPR-Cas system", Nature biotechnology, (20130000), vol. 31, doi:10.1038/nbt.2501, pages 227 - 229, XP055086625

DOI:   http://dx.doi.org/10.1038/nbt.2501
    - JINEK, M. et al., "RNA-programmed genome editing in human cells", eLife, (20130000), doi:10.7554/eLife.00471, page e00471, XP002699851

DOI:   http://dx.doi.org/10.7554/eLife.00471
    - JIANG, W. et al., "RNA-guided editing of bacterial genomes using CRISPR-Cas systems", Nature biotechnology, (20130000), vol. 31, doi:10.1038/nbt.2508, pages 233 - 239, XP055249123

DOI:   http://dx.doi.org/10.1038/nbt.2508
    - "A TALE nuclease architecture for efficient genome editing", Nature Biotechnology, vol. 29, no. 2, pages 143 - 8
    - ZHANG, FENG, "Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription", Nature Biotechnology, (20110200), vol. 29, no. 2, doi:10.1038/nbt.1775, pages 149 - 53, XP055005146

DOI:   http://dx.doi.org/10.1038/nbt.1775
    - GEIPLER, R.SCHOLZE, H.HAHN, S.STREUBEL, J.BONAS, U.BEHRENS, S. E.BOCH, J., "Transcriptional Activators of Human Genes with Programmable DNA-Specificity", PLoS ONE, (20110000), vol. 6, no. 5, page e19509
    - BOCH, JENS, "TALEs of genome targeting", Nature Biotechnology, (20110200), vol. 29, no. 2, doi:10.1038/nbt.1767, pages 135 - 6, XP055077654

DOI:   http://dx.doi.org/10.1038/nbt.1767
    - BOCH, JENS, "Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors", Science, (20091200), vol. 326, no. 5959, pages 1509 - 12
    - MOSCOU, MATTHEW J.ADAM J. BOGDANOVE, "A Simple Cipher Governs DNA Recognition by TAL Effectors", Science, (20091200), vol. 326, no. 5959, page 1501, XP002599998
    - "Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes", Nucleic Acids Research.
    - WEBER, E.GRUETZNER, R.WERNER, S.ENGLER, CMARILLONNET, S., "Assembly of Designer TAL Effectors by Golden Gate Cloning", PLoS ONE, (20110000), vol. 6, no. 5, doi:10.1371/journal.pone.0019722, page e19722, XP009162844

DOI:   http://dx.doi.org/10.1371/journal.pone.0019722
    - KLUG ARHODES D, "Zinc fingers: a novel protein fold for nucleic acid recognition", Cold Spring Harb. Symp. Quant. Biol., (19870000), vol. 52, pages 473 - 82
    - KRISHNA SSMAJUMDAR IGRISHIN NV, "Structural classification of zinc fingers: survey and summary", Nucleic Acids Res., (20030100), vol. 31, no. 2, pages 532 - 50
    - PABO COPEISACH EGRANT RA, "Design and selection of novel cys2His2 Zinc finger proteins", Annual Review of Biochemistry, (20010000), vol. 70, pages 313 - 340, XP003001985
    - JAMIESON ACMILLER JCPABO CO, "Drug discovery with engineered zinc-finger proteins", Nature Reviews Drug Discovery, (20030000), vol. 2, no. 5, doi:10.1038/nrd1087, pages 361 - 368, XP002342391

DOI:   http://dx.doi.org/10.1038/nrd1087
    - LIU QSEGAL DJGHIARA JBBARBAS CF, "Design of polydactyl zinc-finger proteins for unique addressing within complex genomes", Proc. Natl. Acad. Sci. U.S.A., (19970500), vol. 94, no. 11, doi:10.1073/pnas.94.11.5525, XP002169348

DOI:   http://dx.doi.org/10.1073/pnas.94.11.5525
    - PAVLETICH NPPABO CO, "Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A", Science, (19910500), vol. 252, no. 5007, doi:10.1126/science.2028256, pages 809 - 17, XP001094171

DOI:   http://dx.doi.org/10.1126/science.2028256
    - PATTANAYAK, V. et al., Nature Methods, (20110000), vol. 8, pages 765 - 770
    - DOYON, J.B.PATTANAYAK, V.MEYER, C.B.LIU, D.R., "Directed evolution and substrate specificity profile of homing endonuclease I-Scel", Journal of the American Chemical Society, (20060000), vol. 128, doi:10.1021/JA0575191, pages 2477 - 2484, XP002611223

DOI:   http://dx.doi.org/10.1021/JA0575191
    - HOCKEMEYER, D. et al., "Genetic engineering of human pluripotent cells using TALE nucleases", Nature biotechnology, (20110000), vol. 29, doi:10.1038/nbt.1927, pages 731 - 734, XP037152052

DOI:   http://dx.doi.org/10.1038/nbt.1927
    - ZOU, J. et al., "Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells", Cell stem cell, (20090000), vol. 5, doi:10.1016/j.stem.2009.05.023, pages 97 - 110, XP009143029

DOI:   http://dx.doi.org/10.1016/j.stem.2009.05.023
    - HOCKEMEYER, D. et al., "Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases", Nature biotechnology, (20090000), vol. 27, doi:10.1038/nbt.1562, pages 851 - 857, XP055390559

DOI:   http://dx.doi.org/10.1038/nbt.1562
    - DOYON, Y. et al., "Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases", Nature biotechnology, (20080000), vol. 26, doi:10.1038/NBT1409, pages 702 - 708, XP002512110

DOI:   http://dx.doi.org/10.1038/NBT1409
    - MENG, X.NOYES, M.B.ZHU, L.J.LAWSON, N.D.WOLFE, S.A., "Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases", Nature biotechnology
    - SANDER, J.D. et al., "Targeted gene disruption in somatic zebrafish cells using engineered TALENs", Nature biotechnology, (20110000), vol. 29, doi:10.1038/nbt.1934, pages 697 - 698, XP037152056

DOI:   http://dx.doi.org/10.1038/nbt.1934
    - TESSON, L. et al., "Knockout rats generated by embryo microinjection of TALENs", Nature biotechnology, (20110000), vol. 29, pages 695 - 696
    - CUI, X., "Targeted integration in rat and mouse embryos with zinc-finger nucleases", Nature biotechnology, (20110000), vol. 29, doi:10.1038/nbt.1731, pages 64 - 67, XP037104089

DOI:   http://dx.doi.org/10.1038/nbt.1731
    - PEREZ, E.E. et al., "Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases", Nature biotechnology, (20080000), vol. 26, doi:10.1038/nbt1410, pages 808 - 816, XP055024363

DOI:   http://dx.doi.org/10.1038/nbt1410
    - MALI, P. et al., "RNA-guided human genome engineering via Cas9", Science, vol. 339, doi:10.1126/science.1232033, pages 823 - 826, XP055469277

DOI:   http://dx.doi.org/10.1126/science.1232033
    - JINEK, M. et al., "RNA-programmed genome editing in human cells", eLife, vol. 2, doi:10.7554/eLife.00471, page e00471, XP002699851

DOI:   http://dx.doi.org/10.7554/eLife.00471
    - JIANG, W.BIKARD, D.COX, D.ZHANG, F.MARRAFFINI, L.A., "RNA-guided editing of bacterial genomes using CRISPR-Cas systems", Nature biotechnology, vol. 31, doi:10.1038/nbt.2508, pages 233 - 239, XP055323877

DOI:   http://dx.doi.org/10.1038/nbt.2508
    - SAPRANAUSKAS, R. et al., "The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli", Nucleic acids research, (20110000), vol. 39, doi:10.1093/nar/gkr606, pages 9275 - 9282, XP055265024

DOI:   http://dx.doi.org/10.1093/nar/gkr606
    - QI, L.S. et al., "Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression", Cell, (20130000), vol. 152, doi:10.1016/j.cell.2013.02.022, pages 1173 - 1183, XP055346792

DOI:   http://dx.doi.org/10.1016/j.cell.2013.02.022
    - PATTANAYAK, V.RAMIREZ, C.L.JOUNG, J.K.LIU, D.R., "Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection", Nature methods, vol. 8, doi:10.1038/nmeth.1670, pages 765 - 770, XP055650557

DOI:   http://dx.doi.org/10.1038/nmeth.1670
    - SCHNEIDER, T.D.STEPHENS, R.M., "Sequence logos: a new way to display consensus sequences", Nucleic acids research, (19900000), vol. 18, pages 6097 - 6100, XP000608250
 WO2010US55131
The EPO accepts no responsibility for the accuracy of data originating from other authorities; in particular, it does not guarantee that it is complete, up to date or fit for specific purposes.