EP3995591 - NUCLEASE PROFILING SYSTEM [Right-click to bookmark this link] | Status | The patent has been granted Status updated on 08.12.2023 Database last updated on 08.06.2024 | |
Former | Grant of patent is intended Status updated on 26.07.2023 | ||
Former | Request for examination was made Status updated on 11.11.2022 | ||
Former | The application has been published Status updated on 08.04.2022 | Most recent event Tooltip | 08.12.2023 | (Expected) grant | published on 10.01.2024 [2024/02] | Applicant(s) | For all designated states President And Fellows Of Harvard College 17 Quincy Street Cambridge, Massachusetts 02138 / US | [2022/19] | Inventor(s) | 01 /
LIU, David, R. Lexington, MA 02420 / US | 02 /
PATTANAYAK, Vikram Cambridge, MA 02138 / US | [2022/19] | Representative(s) | Atkins, James Gordon John, et al Kilburn & Strode LLP Lacon London 84 Theobalds Road London WC1X 8NL / GB | [2022/19] | Application number, filing date | 21200987.2 | 08.08.2014 | [2022/19] | Priority number, date | US201361864289P | 09.08.2013 Original published format: US 201361864289 P | US201414320370 | 30.06.2014 Original published format: US201414320370 | US201414320413 | 30.06.2014 Original published format: US201414320413 | [2022/19] | Filing language | EN | Procedural language | EN | Publication | Type: | A1 Application with search report | No.: | EP3995591 | Date: | 11.05.2022 | Language: | EN | [2022/19] | Type: | B1 Patent specification | No.: | EP3995591 | Date: | 10.01.2024 | Language: | EN | [2024/02] | Search report(s) | (Supplementary) European search report - dispatched on: | EP | 08.04.2022 | Classification | IPC: | C12Q1/6816, C12Q1/6869 | [2022/19] | CPC: |
C12Q1/6816 (EP,US);
C12Q1/44 (US);
A61K38/465 (US);
A61P31/18 (EP);
A61P35/00 (EP);
C12N9/22 (US);
C12Q1/6869 (EP,US);
C12Q1/6874 (US);
C12Q1/6883 (US);
| C-Set: |
C12Q1/6816, C12Q2521/301, C12Q2522/101, C12Q2535/122, C12Q2563/131, C12Q2563/149 (EP,US);
C12Q1/6869, C12Q2521/301, C12Q2522/101, C12Q2535/122, C12Q2563/131, C12Q2563/149 (US,EP) | Designated contracting states | AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LI, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR [2022/50] |
Former [2022/19] | AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LI, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR | Title | German: | NUKLEASEPROFILIERUNGSSYSTEM | [2022/19] | English: | NUCLEASE PROFILING SYSTEM | [2022/19] | French: | SYSTÈME DE PROFILAGE DE NUCLÉASE | [2022/19] | Examination procedure | 10.11.2022 | Amendment by applicant (claims and/or description) | 10.11.2022 | Examination requested [2022/50] | 10.11.2022 | Date on which the examining division has become responsible | 27.07.2023 | Communication of intention to grant the patent | 05.12.2023 | Fee for grant paid | 05.12.2023 | Fee for publishing/printing paid | 05.12.2023 | Receipt of the translation of the claim(s) | Parent application(s) Tooltip | EP14755947.0 / EP3030650 | EP18199195.1 / EP3486318 | Fees paid | Renewal fee | 05.10.2021 | Renewal fee patent year 03 | 05.10.2021 | Renewal fee patent year 04 | 05.10.2021 | Renewal fee patent year 05 | 05.10.2021 | Renewal fee patent year 06 | 05.10.2021 | Renewal fee patent year 07 | 05.10.2021 | Renewal fee patent year 08 | 29.08.2022 | Renewal fee patent year 09 | 28.08.2023 | Renewal fee patent year 10 |
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Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court. | Documents cited: | Search | [AD]WO2013066438 (HARVARD COLLEGE [US], et al) [AD] 1-20 * the whole document * * para. 10, 95, 96, 102-105, claims 27-38, 70-80 *; | [A] - PRASHANT MALI ET AL, "CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering", NATURE BIOTECHNOLOGY, (20130801), vol. 31, no. 9, doi:10.1038/nbt.2675, ISSN 1087-0156, pages 833 - 838, XP055149275 [A] 1-20 * the whole document * DOI: http://dx.doi.org/10.1038/nbt.2675 | [A] - PATRICK D HSU ET AL, "DNA targeting specificity of RNA-guided Cas9 nucleases", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, UNITED STATES, vol. 31, no. 9, doi:10.1038/NBT.2647, ISSN 1546-1696, (20130721), pages 827 - 832, (20130721), XP002718604 [A] 1-20 * the whole document * DOI: http://dx.doi.org/10.1038/nbt.2647 | [IP] - JOHN P GUILINGER ET AL, "Broad specificity profiling of TALENs results in engineered nucleases with improved DNA-cleavage specificity", NATURE METHODS, (20140216), vol. 11, no. 4, doi:10.1038/nmeth.2845, ISSN 1548-7091, pages 429 - 435, XP055148794 [IP] 1-20 * the whole document * DOI: http://dx.doi.org/10.1038/nmeth.2845 | [IP] - VIKRAM PATTANAYAK ET AL, "High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity", NATURE BIOTECHNOLOGY, (20130811), vol. 31, no. 9, doi:10.1038/nbt.2673, ISSN 1087-0156, pages 839 - 843, XP055148795 [IP] 1-20 * the whole document * DOI: http://dx.doi.org/10.1038/nbt.2673 | by applicant | WO2013066438 | - PATTANAYAK, V.RAMIREZ, C.L.JOUNG, J.K.LIU, D.R., "Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection", Nature methods, (20110000), vol. 8, doi:10.1038/nmeth.1670, pages 765 - 770, XP055650557 DOI: http://dx.doi.org/10.1038/nmeth.1670 | - JINEK M.CHYLINSKI K.FONFARA I.HAUER M.DOUDNA J.A.CHARPENTIER E., "A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity", Science, (20120000), vol. 337, doi:10.1126/science.1225829, pages 816 - 821, XP055229606 DOI: http://dx.doi.org/10.1126/science.1225829 | - FERRETTI J.J.MCSHAN W.M.AJDIC D.J.SAVIC D.J.SAVIC G.LYON K.PRIMEAUX C.SEZATE S.SUVOROV A.N.KENTON S., "Complete genome sequence of an M1 strain of Streptococcus pyogenes", Proc. Natl. Acad. Sci. U.S.A., (20010000), vol. 98, doi:10.1073/pnas.071559398, pages 4658 - 4663, XP002344854 DOI: http://dx.doi.org/10.1073/pnas.071559398 | - DELTCHEVA E.CHYLINSKI K.SHARMA C.M.GONZALES K.CHAO Y.PIRZADA Z.A.ECKERT M.R.VOGEL J.CHARPENTIER E., "CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III", Nature, (20110000), vol. 471, doi:10.1038/nature09886, pages 602 - 607, XP055308803 DOI: http://dx.doi.org/10.1038/nature09886 | - CHYLINSKIRHUNCHARPENTIER, "The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems", RNA Biology, (20130000), vol. 10, no. 5, doi:10.4161/rna.24321, pages 726 - 737, XP055116068 DOI: http://dx.doi.org/10.4161/rna.24321 | - K. C. NICOLAOUA. L. SMITHE. W. YUE, "Chemistry and biology of natural and designed enediynes", PNAS, (19930000), vol. 90, no. 13, pages 5881 - 5888 | - S. WALKERR. LANDOVITZW.D. DINGG.A. ELLESTADD. KAHNE, "Cleavage behavior of calicheamicin gamma 1 and calicheamicin T", Proc Natl Acad Sci U.S.A., (19920000), vol. 89, no. 10, pages 4608 - 12 | - ADRIAN L. SMITHK. C. BICOLAOU, "The Enediyne Antibiotics", J. Med. Chem., (19960000), vol. 39, no. 11, pages 2103 - 2117 | - EDGELL DR, "Selfish DNA: homing endonucleases find a home", Curr Biol, (20090200), vol. 19, no. 3, doi:10.1016/j.cub.2008.12.019, pages R115 - R117, XP025916026 DOI: http://dx.doi.org/10.1016/j.cub.2008.12.019 | - JASIN M, "Genetic manipulation of genomes with rare-cutting endonucleases", Trends Genet, (19960600), vol. 12, no. 6, doi:10.1016/0168-9525(96)10019-6, pages 224 - 8, XP022519434 DOI: http://dx.doi.org/10.1016/0168-9525(96)10019-6 | - BURT AKOUFOPANOU V, "Homing endonuclease genes: the rise and fall and rise again of a selfish element", Curr Opin Genet Dev, (20041200), vol. 14, no. 6, doi:10.1016/j.gde.2004.09.010, pages 609 - 15, XP004628734 DOI: http://dx.doi.org/10.1016/j.gde.2004.09.010 | - CONG, L. et al., "Multiplex genome engineering using CRISPR/Cas systems", Science, (20130000), vol. 339, doi:10.1126/science.1231143, pages 819 - 823, XP055458249 DOI: http://dx.doi.org/10.1126/science.1231143 | - MALI, P. et al., "RNA-guided human genome engineering via Cas9", Science, (20130000), vol. 339, doi:10.1126/science.1232033, pages 823 - 826, XP055469277 DOI: http://dx.doi.org/10.1126/science.1232033 | - HWANG, W.Y. et al., "Efficient genome editing in zebrafish using a CRISPR-Cas system", Nature biotechnology, (20130000), vol. 31, doi:10.1038/nbt.2501, pages 227 - 229, XP055086625 DOI: http://dx.doi.org/10.1038/nbt.2501 | - JINEK, M. et al., "RNA-programmed genome editing in human cells", eLife, (20130000), doi:10.7554/eLife.00471, page e00471, XP002699851 DOI: http://dx.doi.org/10.7554/eLife.00471 | - JIANG, W. et al., "RNA-guided editing of bacterial genomes using CRISPR-Cas systems", Nature biotechnology, (20130000), vol. 31, doi:10.1038/nbt.2508, pages 233 - 239, XP055249123 DOI: http://dx.doi.org/10.1038/nbt.2508 | - "A TALE nuclease architecture for efficient genome editing", Nature Biotechnology, vol. 29, no. 2, pages 143 - 8 | - ZHANG, FENG, "Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription", Nature Biotechnology, (20110200), vol. 29, no. 2, doi:10.1038/nbt.1775, pages 149 - 53, XP055005146 DOI: http://dx.doi.org/10.1038/nbt.1775 | - GEIPLER, R.SCHOLZE, H.HAHN, S.STREUBEL, J.BONAS, U.BEHRENS, S. E.BOCH, J., "Transcriptional Activators of Human Genes with Programmable DNA-Specificity", PLoS ONE, (20110000), vol. 6, no. 5, page e19509 | - BOCH, JENS, "TALEs of genome targeting", Nature Biotechnology, (20110200), vol. 29, no. 2, doi:10.1038/nbt.1767, pages 135 - 6, XP055077654 DOI: http://dx.doi.org/10.1038/nbt.1767 | - BOCH, JENS, "Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors", Science, (20091200), vol. 326, no. 5959, pages 1509 - 12 | - MOSCOU, MATTHEW J.ADAM J. BOGDANOVE, "A Simple Cipher Governs DNA Recognition by TAL Effectors", Science, (20091200), vol. 326, no. 5959, page 1501, XP002599998 | - "Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes", Nucleic Acids Research. | - WEBER, E.GRUETZNER, R.WERNER, S.ENGLER, CMARILLONNET, S., "Assembly of Designer TAL Effectors by Golden Gate Cloning", PLoS ONE, (20110000), vol. 6, no. 5, doi:10.1371/journal.pone.0019722, page e19722, XP009162844 DOI: http://dx.doi.org/10.1371/journal.pone.0019722 | - KLUG ARHODES D, "Zinc fingers: a novel protein fold for nucleic acid recognition", Cold Spring Harb. Symp. Quant. Biol., (19870000), vol. 52, pages 473 - 82 | - KRISHNA SSMAJUMDAR IGRISHIN NV, "Structural classification of zinc fingers: survey and summary", Nucleic Acids Res., (20030100), vol. 31, no. 2, pages 532 - 50 | - PABO COPEISACH EGRANT RA, "Design and selection of novel cys2His2 Zinc finger proteins", Annual Review of Biochemistry, (20010000), vol. 70, pages 313 - 340, XP003001985 | - JAMIESON ACMILLER JCPABO CO, "Drug discovery with engineered zinc-finger proteins", Nature Reviews Drug Discovery, (20030000), vol. 2, no. 5, doi:10.1038/nrd1087, pages 361 - 368, XP002342391 DOI: http://dx.doi.org/10.1038/nrd1087 | - LIU QSEGAL DJGHIARA JBBARBAS CF, "Design of polydactyl zinc-finger proteins for unique addressing within complex genomes", Proc. Natl. Acad. Sci. U.S.A., (19970500), vol. 94, no. 11, doi:10.1073/pnas.94.11.5525, XP002169348 DOI: http://dx.doi.org/10.1073/pnas.94.11.5525 | - PAVLETICH NPPABO CO, "Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A", Science, (19910500), vol. 252, no. 5007, doi:10.1126/science.2028256, pages 809 - 17, XP001094171 DOI: http://dx.doi.org/10.1126/science.2028256 | - PATTANAYAK, V. et al., Nature Methods, (20110000), vol. 8, pages 765 - 770 | - DOYON, J.B.PATTANAYAK, V.MEYER, C.B.LIU, D.R., "Directed evolution and substrate specificity profile of homing endonuclease I-Scel", Journal of the American Chemical Society, (20060000), vol. 128, doi:10.1021/JA0575191, pages 2477 - 2484, XP002611223 DOI: http://dx.doi.org/10.1021/JA0575191 | - HOCKEMEYER, D. et al., "Genetic engineering of human pluripotent cells using TALE nucleases", Nature biotechnology, (20110000), vol. 29, doi:10.1038/nbt.1927, pages 731 - 734, XP037152052 DOI: http://dx.doi.org/10.1038/nbt.1927 | - ZOU, J. et al., "Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells", Cell stem cell, (20090000), vol. 5, doi:10.1016/j.stem.2009.05.023, pages 97 - 110, XP009143029 DOI: http://dx.doi.org/10.1016/j.stem.2009.05.023 | - HOCKEMEYER, D. et al., "Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases", Nature biotechnology, (20090000), vol. 27, doi:10.1038/nbt.1562, pages 851 - 857, XP055390559 DOI: http://dx.doi.org/10.1038/nbt.1562 | - DOYON, Y. et al., "Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases", Nature biotechnology, (20080000), vol. 26, doi:10.1038/NBT1409, pages 702 - 708, XP002512110 DOI: http://dx.doi.org/10.1038/NBT1409 | - MENG, X.NOYES, M.B.ZHU, L.J.LAWSON, N.D.WOLFE, S.A., "Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases", Nature biotechnology | - SANDER, J.D. et al., "Targeted gene disruption in somatic zebrafish cells using engineered TALENs", Nature biotechnology, (20110000), vol. 29, doi:10.1038/nbt.1934, pages 697 - 698, XP037152056 DOI: http://dx.doi.org/10.1038/nbt.1934 | - TESSON, L. et al., "Knockout rats generated by embryo microinjection of TALENs", Nature biotechnology, (20110000), vol. 29, pages 695 - 696 | - CUI, X., "Targeted integration in rat and mouse embryos with zinc-finger nucleases", Nature biotechnology, (20110000), vol. 29, doi:10.1038/nbt.1731, pages 64 - 67, XP037104089 DOI: http://dx.doi.org/10.1038/nbt.1731 | - PEREZ, E.E. et al., "Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases", Nature biotechnology, (20080000), vol. 26, doi:10.1038/nbt1410, pages 808 - 816, XP055024363 DOI: http://dx.doi.org/10.1038/nbt1410 | - MALI, P. et al., "RNA-guided human genome engineering via Cas9", Science, vol. 339, doi:10.1126/science.1232033, pages 823 - 826, XP055469277 DOI: http://dx.doi.org/10.1126/science.1232033 | - JINEK, M. et al., "RNA-programmed genome editing in human cells", eLife, vol. 2, doi:10.7554/eLife.00471, page e00471, XP002699851 DOI: http://dx.doi.org/10.7554/eLife.00471 | - JIANG, W.BIKARD, D.COX, D.ZHANG, F.MARRAFFINI, L.A., "RNA-guided editing of bacterial genomes using CRISPR-Cas systems", Nature biotechnology, vol. 31, doi:10.1038/nbt.2508, pages 233 - 239, XP055323877 DOI: http://dx.doi.org/10.1038/nbt.2508 | - SAPRANAUSKAS, R. et al., "The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli", Nucleic acids research, (20110000), vol. 39, doi:10.1093/nar/gkr606, pages 9275 - 9282, XP055265024 DOI: http://dx.doi.org/10.1093/nar/gkr606 | - QI, L.S. et al., "Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression", Cell, (20130000), vol. 152, doi:10.1016/j.cell.2013.02.022, pages 1173 - 1183, XP055346792 DOI: http://dx.doi.org/10.1016/j.cell.2013.02.022 | - PATTANAYAK, V.RAMIREZ, C.L.JOUNG, J.K.LIU, D.R., "Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection", Nature methods, vol. 8, doi:10.1038/nmeth.1670, pages 765 - 770, XP055650557 DOI: http://dx.doi.org/10.1038/nmeth.1670 | - SCHNEIDER, T.D.STEPHENS, R.M., "Sequence logos: a new way to display consensus sequences", Nucleic acids research, (19900000), vol. 18, pages 6097 - 6100, XP000608250 | WO2010US55131 |