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Extract from the Register of European Patents

EP About this file: EP4121524

EP4121524 - OPTIMISED METHODS FOR CLEAVAGE OF TARGET SEQUENCES [Right-click to bookmark this link]
StatusRequest for examination was made
Status updated on  23.12.2022
Database last updated on 08.10.2024
FormerThe international publication has been made
Status updated on  24.09.2021
Formerunknown
Status updated on  29.03.2021
Most recent event   Tooltip08.03.2024New entry: Renewal fee paid 
Applicant(s)For all designated states
Cancer Research Technology Limited
2 Redman Place
London E20 1JQ / GB
[2023/04]
Inventor(s)01 / RUSSELL, Iain Alasdair
11 Headington Drive
Cambridge CB1 9HE / GB
 [2023/04]
Representative(s)HGF
HGF Limited
1 City Walk
Leeds LS11 9DX / GB
[2023/04]
Application number, filing date21713109.316.03.2021
[2023/04]
WO2021GB50650
Priority number, dateGB2020000381416.03.2020         Original published format: GB 202003814
[2023/04]
Filing languageEN
Procedural languageEN
PublicationType: A1 Application with search report
No.:WO2021186163
Date:23.09.2021
Language:EN
[2021/38]
Type: A1 Application with search report 
No.:EP4121524
Date:25.01.2023
Language:EN
The application published by WIPO in one of the EPO official languages on 23.09.2021 takes the place of the publication of the European patent application.
[2023/04]
Search report(s)International search report - published on:EP23.09.2021
ClassificationIPC:C12N9/22, C12N15/11, A61K48/00
[2023/04]
CPC:
C12N9/22 (EP,US); C12N15/113 (US); C07K14/7051 (EP);
C12N15/102 (EP,US); C12N15/11 (EP); C12N15/907 (EP);
C12N2310/20 (EP,US); C12N2320/11 (EP,US) (-)
Designated contracting statesAL,   AT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   LV,   MC,   MK,   MT,   NL,   NO,   PL,   PT,   RO,   RS,   SE,   SI,   SK,   SM,   TR [2023/04]
TitleGerman:OPTIMIERTE VERFAHREN ZUR SPALTUNG VON ZIELSEQUENZEN[2023/04]
English:OPTIMISED METHODS FOR CLEAVAGE OF TARGET SEQUENCES[2023/04]
French:PROCÉDÉS OPTIMISÉS DE CLIVAGE DE SÉQUENCES CIBLES[2023/04]
Entry into regional phase26.09.2022National basic fee paid 
26.09.2022Designation fee(s) paid 
26.09.2022Examination fee paid 
Examination procedure26.09.2022Examination requested  [2023/04]
26.09.2022Date on which the examining division has become responsible
26.04.2023Amendment by applicant (claims and/or description)
Fees paidRenewal fee
16.03.2023Renewal fee patent year 03
07.03.2024Renewal fee patent year 04
Opt-out from the exclusive  Tooltip
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Patent Court		 See the Register of the Unified Patent Court for opt-out data
Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Cited inInternational search[A]WO2017147056  (CARIBOU BIOSCIENCES INC [US]) [A] 1-28 * Whole document, especially the claims. *;
 [A]WO2019232494  (SYNTHEGO CORP [US]) [A] 1-28 * Whole document,especially the claims *;
    [XDAI] - MEHRAVAR MARYAM ET AL, "Mosaicism in CRISPR/Cas9-mediated genome editing", DEVELOPMENTAL BIOLOGY, AMSTERDAM, NL, (20181022), vol. 445, no. 2, doi:10.1016/j.ydbio.2018.10.008, ISSN 0012-1606, pages 156 - 162, XP055811002 [XD] 28 * the whole document * [A] 2-27 [I] 1

DOI:   http://dx.doi.org/10.1016/j.ydbio.2018.10.008
 [A]  - Grajcarek Janin ET AL, "Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations", Nature communications, England, doi:10.1038/s41467-019-12829-8, (20191024), URL: https://www.nature.com/articles/s41467-019-12829-8.pdf, (20210607), XP055810962 [A] 1-28 * the whole document *

DOI:   http://dx.doi.org/10.1038/s41467-019-12829-8
 [A]  - CHEN WEI ET AL, "Massively parallel profiling and predictive modelling of the outcomes of CRISPR/Cas9-mediated double-strand break repair", NUCLEIC ACIDS RESEARCH, GB, vol. 47, no. 15, doi:10.1093/nar/gkz487, ISSN 0305-1048, (20190605), pages 7989 - 8003, URL: http://academic.oup.com/nar/advance-article-pdf/doi/10.1093/nar/gkz487/28767838/gkz487.pdf, XP055795043 [A] 1-28

DOI:   http://dx.doi.org/10.1093/nar/gkz487
 [AD]  - PATRICK D HSU ET AL, "DNA targeting specificity of RNA-guided Cas9 nucleases (includes online Methods)", NATURE BIOTECHNOLOGY, New York, (20130721), vol. 31, no. 9, doi:10.1038/nbt.2647, ISSN 1087-0156, pages 827 - 832+2PP, XP055382777 [AD] 1-28

DOI:   http://dx.doi.org/10.1038/nbt.2647
 [A]  - DAQI WANG ET AL, "Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning", NATURE COMMUNICATIONS, (20190919), vol. 10, no. 1, doi:10.1038/s41467-019-12281-8, XP055631062 [A] 1-28 * the whole document *

DOI:   http://dx.doi.org/10.1038/s41467-019-12281-8
 [AD]  - MIGUEL A MORENO-MATEOS ET AL, "CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo", NATURE METHODS, New York, (20150831), vol. 12, no. 10, doi:10.1038/nmeth.3543, ISSN 1548-7091, pages 982 - 988, XP055284090 [AD] 1-28

DOI:   http://dx.doi.org/10.1038/nmeth.3543
 [AD]  - SHEN MAX W ET AL, "Predictable and precise template-free CRISPR editing of pathogenic variants", NATURE, MACMILLAN JOURNALS LTD., ETC, LONDON, vol. 563, no. 7733, doi:10.1038/S41586-018-0686-X, ISSN 0028-0836, (20181107), pages 646 - 651, (20181107), XP036703023 [AD] 1-28

DOI:   http://dx.doi.org/10.1038/s41586-018-0686-x
 [A]  - S BAE ET AL, "Microhom ology-based choice of Cas9 nuclease target sites", NAT. METHODS, (20140101), vol. 11, no. 7, pages 705 - 706, XP055233413 [A] 1-28 * the whole document *
 [A]  - ALIAKSANDRA RADZISHEUSKAYA ET AL, "Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression", NUCLEIC ACIDS RESEARCH, GB, (20160628), vol. 44, no. 18, doi:10.1093/nar/gkw583, ISSN 0305-1048, pages e141 - e141, XP055563489 [A] 1-28 * the whole document *

DOI:   http://dx.doi.org/10.1093/nar/gkw583
by applicant   - LAMAS-TORAZO et al., Nature Scientific Reports, (20190000), vol. 9
    - MEHRAVAR et al., Dev Biol., (20190115), vol. 445, no. 2, pages 156 - 162
    - ALLEN, Nature Biotechnology, (20190000), vol. 37, pages 64 - 72
    - SHEN et al., Nature, (20180000), vol. 563, page 646
    - LINDEL, Nucleic Acids Research, (20190000), vol. 47, pages 7989 - 8003
    - HSU et al., Nature Biotechnology, (20130000), vol. 31, pages 827 - 832
    - DOENCH et al., Nature Biotechnology, (20160000), vol. 34, pages 184 - 191
    - MORENO-MATEOS et al., Nature Methods, (20150000), vol. 12, pages 982 - 988
    - TZELEPIS et al., Cell Reports, (20161018), vol. 17, no. 4
The EPO accepts no responsibility for the accuracy of data originating from other authorities; in particular, it does not guarantee that it is complete, up to date or fit for specific purposes.