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EP4204558
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| EP4204558 - CRISPR/CAS9 MULTIPLEX KNOCKOUT OF HOST CELL PROTEINS [Right-click to bookmark this link] | Status | Request for examination was made Status updated on 02.06.2023 Database last updated on 12.07.2024 | |
| Former | The international publication has been made Status updated on 05.03.2022 | ||
| Former | unknown Status updated on 19.10.2021 | Most recent event Tooltip | 03.11.2023 | Change: Validation states | published on 06.12.2023 [2023/49] | 03.11.2023 | Change - extension states | published on 06.12.2023 [2023/49] | Applicant(s) | For all designated states GENENTECH, INC. 1 DNA Way South San Francisco, CA 94080 / US | For all designated states F. Hoffmann-La Roche AG Grenzacherstrasse 124 4070 Basel / CH | [2023/27] | Inventor(s) | 01 /
SHEN, Amy South San Francisco, CA 94080-4990 / US | 02 /
YUK, Inn, Huam South San Francisco, CA 94080-4990 / US | 03 /
KO, Peggy Wai No South San Francisco, CA 94080-4990 / US | 04 /
MISAGHI, Shahram South San Francisco, CA 94080-4990 / US | 05 /
AUSLAENDER, Simon Thomas 82377 Penzberg / DE | 06 /
GREENWOOD-GOODWIN, Midori South San Francisco, CA 94080-4990 / US | 07 /
LAIRD, Michael Wilson South San Francisco, CA 94080-4990 / US | 08 /
OSWALD, Benedikt Alois Claudius 82377 Penzberg / DE | [2023/27] | Representative(s) | Buser, Andres, et al F. Hoffmann-La Roche AG Patent Department Grenzacherstrasse 124 4070 Basel / CH | [2023/27] | Application number, filing date | 21786288.7 | 27.08.2021 | [2023/27] | WO2021US48046 | Priority number, date | US202063071764P | 28.08.2020 Original published format: US 202063071764 P | [2023/27] | Filing language | EN | Procedural language | EN | Publication | Type: | A2 Application without search report | No.: | WO2022047222 | Date: | 03.03.2022 | Language: | EN | [2022/09] | Type: | A2 Application without search report | No.: | EP4204558 | Date: | 05.07.2023 | Language: | EN | The application published by WIPO in one of the EPO official languages on 03.03.2022 takes the place of the publication of the European patent application. | [2023/27] | Search report(s) | International search report - published on: | EP | 14.04.2022 | Classification | IPC: | C12N15/10, C12N15/85, C12N15/90 | [2023/27] | CPC: |
C12N15/907 (EP,KR,US);
C12N15/1082 (US);
C12N15/102 (EP,KR);
C12N15/11 (US);
C12N15/113 (EP,KR);
C12N5/00 (US);
C12N5/0682 (KR);
C12N9/22 (EP,KR,US);
C12P21/02 (EP);
| Designated contracting states | AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LI, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR [2023/27] | Title | German: | CRISPR/CAS9-MULTIPLEX-KNOCKOUT VON WIRTSZELLENPROTEINEN | [2023/27] | English: | CRISPR/CAS9 MULTIPLEX KNOCKOUT OF HOST CELL PROTEINS | [2023/27] | French: | INACTIVATION DE PROTÉINES DE CELLULES HÔTES PAR LE MULTIPLEXE DE CRISPR/CAS9 | [2023/27] | Entry into regional phase | 28.03.2023 | National basic fee paid | 28.03.2023 | Designation fee(s) paid | 28.03.2023 | Examination fee paid | Examination procedure | 28.03.2023 | Examination requested [2023/27] | 28.03.2023 | Date on which the examining division has become responsible | 26.09.2023 | Amendment by applicant (claims and/or description) | Fees paid | Renewal fee | 22.08.2023 | Renewal fee patent year 03 |
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| Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court. | Cited in | International search | [A]WO2020163365 (KSQ THERAPEUTICS INC [US]) [A] 1-33 * claims 1-4,10,11,43-45 *; | [E]WO2021195464 (GENENTECH INC [US]) [E] 1-33 * figure 2; examples 2-5 *; | [A] - KWANGRYUL BAEK ET AL, "DNA-free two-gene knockout in Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins", SCIENTIFIC REPORTS, (20160728), vol. 6, no. 1, doi:10.1038/srep30620, pages 1 - 7, XP055435475 [A] 1-33 * page 3; figures 1,3 * DOI: http://dx.doi.org/10.1038/srep30620 | [Y] - SHI XIANGGUO ET AL, "Clonal expansion and myeloid leukemia progression modeled by multiplex gene editing of murine hematopoietic progenitor cells", EXPERIMENTAL HEMATALOGY, ELSEVIER INC, US, (20180508), vol. 64, doi:10.1016/J.EXPHEM.2018.04.009, ISSN 0301-472X, pages 33 - 44, XP085429045 [Y] 1-33 * page 37, column l - page 41, column r; figures 1; E2, E4 * DOI: http://dx.doi.org/10.1016/j.exphem.2018.04.009 | [Y] - HOELLERBAUER PIA ET AL, "A simple and highly efficient method for multi-allelic CRISPR-Cas9 editing in primary cell cultures", CANCER REPORTS, vol. 3, no. 5, doi:10.1002/cnr2.1269, ISSN 2573-8348, (20200728), URL: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/cnr2.1269, XP055875456 [Y] 1-33 * page 10, column r - page 11, column r; figures 1,3 * DOI: http://dx.doi.org/10.1002/cnr2.1269 | [ ] - Hoellerbauer Pia ET AL, "SUPPORTING INFORMATION for A simple and highly efficient method for multi-allelic CRISPR-Cas9 editing in primary cell cultures", Cancer Reports, (20200728), URL: https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fcnr2.1269&file=cnr21269-sup-0001-Supinfo1.docx, (20211222), XP055875461 [ ] * figure S1 * | [ ] - Hoellerbauer Pia ET AL, "SUPPORTING INFORMATION: Protocol for Cas9:sgRNA ribonucleoprotein nucleofection using Amaxa 4D Nucleofector X Unit or 96-well Shuttle System", Cancer Reports, (20200728), URL: https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fcnr2.1269&file=cnr21269-sup-0002-Supinfo2.docx, (20211222), XP055875462 [ ] * page 2 - page 6 * | [Y] - SAFARI FATEMEH ET AL, "Multiplex Genome Editing in Chinese Hamster Ovary Cell Line Using All-in-One and HITI CRISPR Technology", ADVANCED PHARMACEUTICAL BULLETIN, vol. 11, no. 2, doi:10.34172/apb.2021.032, ISSN 2228-5881, (20200415), pages 343 - 350, URL: https://apb.tbzmed.ac.ir/Files/Inpress/apb-28493.pdf, XP055874369 [Y] 1-33 * page 345 * * page 347, column l * * abstract * DOI: http://dx.doi.org/10.34172/apb.2021.032 | [A] - JOOSU KUIVANEN ET AL, "Development of microtiter plate scale CRISPR/Cas9 transformation method for Aspergillus niger based on in vitro assembled ribonucleoprotein complexes", FUNGAL BIOLOGY AND BIOTECHNOLOGY, (20190315), vol. 6, no. 1, doi:10.1186/s40694-019-0066-9, XP055683175 [A] 1-33 * figures 3,4; table 2 * DOI: http://dx.doi.org/10.1186/s40694-019-0066-9 | [A] - CHO JAE SUNG ET AL, "CRISPR/Cas9-coupled recombineering for metabolic engineering of Corynebacterium glutamicum", METABOLIC ENGINEERING, ACADEMIC PRESS, AMSTERDAM, NL, (20170623), vol. 42, doi:10.1016/J.YMBEN.2017.06.010, ISSN 1096-7176, pages 157 - 167, XP085136204 [A] 1-33 * page 159, column r; figure 4 * DOI: http://dx.doi.org/10.1016/j.ymben.2017.06.010 | [A] - JU ANNA ET AL, "A carrier-free multiplexed gene editing system applicable for suspension cells", BIOMATERIALS, ELSEVIER, AMSTERDAM, NL, vol. 217, doi:10.1016/J.BIOMATERIALS.2019.119298, ISSN 0142-9612, (20190625), (20190625), XP085743178 [A] 1-33 * figures 1,3-5 * DOI: http://dx.doi.org/10.1016/j.biomaterials.2019.119298 | [A] - SOARES MEDEIROS LIA CAROLINA ET AL, "Rapid, Selection-Free, High-Efficiency Genome Editing in Protozoan Parasites Using CRISPR-Cas9 Ribonucleoproteins", MBIO, US, vol. 8, no. 6, doi:10.1128/mBio.01788-17, ISSN 2161-2129, (20171229), URL: https://journals.asm.org/doi/pdf/10.1128/mBio.01788-17, XP055873623 [A] 1-33 * page 11; figure 1 * DOI: http://dx.doi.org/10.1128/mBio.01788-17 | [A] - ZHANG WEN-WEI ET AL, "Optimized CRISPR-Cas9 Genome Editing for Leishmania and its use to target a multigene family, induce chromosomal translocation, and study DNA break repair mechanisms", MSPHERE, (20170118), vol. 2, no. 1, doi:10.1128/mSphere.00340-16, XP055790321 [A] 1-33 * figure 1 * DOI: http://dx.doi.org/10.1128/mSphere.00340-16 | [A] - WEI TUO ET AL, "Systemic nanoparticle delivery of CRISPR-Cas9 ribonucleoproteins for effective tissue specific genome editing", NATURE COMMUNICATIONS, vol. 11, no. 1, doi:10.1038/s41467-020-17029-3, (20200626), URL: https://www.nature.com/articles/s41467-020-17029-3.pdf, XP055873647 [A] 1-33 * page 7, column l; figures 3,4 * DOI: http://dx.doi.org/10.1038/s41467-020-17029-3 | [A] - DAVID B. THOMPSON ET AL, "The Future of Multiplexed Eukaryotic Genome Engineering", ACS CHEMICAL BIOLOGY, (20171214), vol. 13, no. 2, doi:10.1021/acschembio.7b00842, ISSN 1554-8929, pages 313 - 325, XP055711612 [A] 1-33 * the whole document * DOI: http://dx.doi.org/10.1021/acschembio.7b00842 | [A] - JAE SEONG LEE ET AL, "CRISPR/Cas9-mediated genome engineering of CHO cell factories: Application and perspectives", BIOTECHNOLOGY JOURNAL, DE, (20150609), vol. 10, no. 7, doi:10.1002/biot.201500082, ISSN 1860-6768, pages 979 - 994, XP055372758 [A] 1-33 * table 1 * DOI: http://dx.doi.org/10.1002/biot.201500082 | [T] - CARVER JOSEPH ET AL, "A ribonucleoprotein-based decaplex CRISPR/Cas9 knockout strategy for CHO host engineering", BIOTECHNOLOGY PROGRESS, doi:10.1002/btpr.3212, ISSN 8756-7938, (20210919), URL: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/btpr.3212, XP055873550 [T] 1-33 * the whole document * DOI: http://dx.doi.org/10.1002/btpr.3212 | by applicant | US830930 | US4560655 | WO8700195 | US4657866 | US4676980 | US4767704 | EP0307247 | US4816567 | WO9003430 | US4927762 | EP0404097 | US5122469 | WO9301161 | US5208020 | WO9316185 | WO9411026 | WO9429351 | US5500362 | US5571894 | US5587458 | US5624821 | US5648260 | WO9730087 | US5731168 | US5770429 | US5821337 | WO9850431 | WO9858964 | US5869046 | WO9922764 | WO9951642 | WO9954342 | US6075181 | US6150584 | US6194551 | US6248516 | WO0177342 | WO03011878 | US2003157108 | WO03085107 | US2004093621 | US6737056 | US2004110282 | WO2004056312 | US2004132140 | WO2004065540 | WO2004106381 | US2004259150 | US2005014934 | US2005031613 | WO2005061547 | WO2005100402 | US6982321 | WO2006029879 | US7041870 | US7087409 | WO2006082515 | US7189826 | US2007061900 | WO2007042261 | US7332581 | WO2008024715 | US2008069820 | US7371826 | WO2008119567 | US7521541 | US7527791 | WO2009080252 | WO2009080253 | WO2009080251 | WO2009089004 | WO2010112193 | WO2010115589 | WO2010136172 | US7855275 | WO2010145792 | WO2011034605 | WO2012130831 | WO2013026831 | WO2013026833 | WO2013026839 | WO2013120929 | WO2014177460 | US9000130 | WO2015095539 | WO2015148806 | WO2015150447 | WO2015153513 | WO2016016299 | WO2016020309 | WO2016040856 | US2016176954 | WO2016172485 | EP2101823 | - LALONDE et al., Journal of Biotechnology, (20170000), vol. 251, pages 128 - 140 | - KUNERT et al., Appl Microbiol Biotechnol, (20160000), vol. 100, no. 8, pages 3451 - 3461 | - WELLS et al., Biotechnology Journal, (20170000), vol. 12, no. 1, page 1600105 | - CHIU et al., Biotechnology and Bioengineering, (20170000), vol. 114, no. 5, pages 1006 - 1015 | - GRAV et al., Biotechnology Journal, (20150000), vol. 10, no. 9, pages 1446 - 1456 | - LAUX et al., Biotechnology and Bioengineering, (20180000), vol. 115, no. 10, pages 2530 - 2540 | - KOL et al., Nature Communications, (20200000), vol. 11, no. 1, pages 1 - 10 | - WALKER et al., Heterologous Protein Production in CHO Cells, Taylor & Francis, (20170000), vol. 9, pages 101 - 118 | - AMANN et al., "Deca CHO KO: exploring the limitations of CRISPR/Cas9 multiplexing in CHO cells", Design of Optimal CHO Protein N-glycosylation Profiles, (20180000), page 36 | - SERGEEVA et al., CRISPR Gene Editing, Springer, (20190000), pages 213 - 232 | - VANLEUVEN et al., Biotechniques, (20180000), vol. 64, no. 6, pages 275 - 278 | - SENTMANAT et al., Scientific Reports, (20180000), vol. 8, no. 1, pages 1 - 8 | - LINO et al., Drug Deliv., (20180000), vol. 25, no. 1, pages 1234 - 1257 | - MORRISON et al., Proc. 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