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EP4586303
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| EP4586303 - A METHOD AND APPARATUS OF PREPARING A SAMPLE OF ONE OR MORE MOLECULE(S) FOR IMAGING WITH A CRYO-ELECTRON MICROSCOPE [Right-click to bookmark this link] | Status | Grant of patent is intended Status updated on 27.02.2026 Database last updated on 19.03.2026 | |
| Former | Request for examination was made Status updated on 17.10.2025 | ||
| Former | The application has been published Status updated on 13.06.2025 | Most recent event Tooltip | 27.02.2026 | New entry: Communication of intention to grant a patent | 27.02.2026 | Change - inventor | published on 01.04.2026 [2026/14] | Applicant(s) | For all designated states FEI COMPANY 5350 NE Dawson Creek Drive Hillsboro, OR 97124 / US | For all designated states Oxford University Innovation Limited Buxton Court 3 West Way Oxford, Oxfordshire OX2 0JB / GB | [2025/29] | Inventor(s) | 01 /
RAUSCHENBACH, Stephan Oxford OX2 0JB / GB | 02 /
ESSER, Tim Oxford OX2 0JB / GB | 03 /
FREMDLING, Paul Oxford OX2 0JB / GB | 04 /
GAULT, Joe Oxford OX2 0JB / GB | 05 /
KONIJNENBERG, Albert Hillsboro 97124 / US | 06 /
BAHM, Adam Hillsboro 97124 / US | [2026/14] |
| Former [2025/29] | 01 /
RAUSCHENBACH, Stephan Oxford, OX2 0JB / GB | ||
| 02 /
ESSER, Tim Oxford, OX2 0JB / GB | |||
| 03 /
FREMDLING, Paul Oxford, OX2 0JB / GB | |||
| 04 /
GAULT, Joe Oxford, OX2 0JB / GB | |||
| 05 /
KONIJNENBERG, Albert Hillsboro, 97124 / US | |||
| 06 /
BAHM, Adam Hillsboro, 97124 / US | Representative(s) | Boult Wade Tennant LLP Salisbury Square House 8 Salisbury Square London EC4Y 8AP / GB | [2025/29] | Application number, filing date | 25179416.0 | 16.12.2022 | [2025/29] | Priority number, date | GB20210019116 | 30.12.2021 Original published format: GB 202119116 | [2025/29] | Filing language | EN | Procedural language | EN | Publication | Type: | A2 Application without search report | No.: | EP4586303 | Date: | 16.07.2025 | Language: | EN | [2025/29] | Type: | A3 Search report | No.: | EP4586303 | Date: | 06.08.2025 | Language: | EN | [2025/32] | Search report(s) | (Supplementary) European search report - dispatched on: | EP | 04.07.2025 | Classification | IPC: | G01N1/28, G01N1/42, G01N27/623, H01J49/06 | [2025/32] | CPC: |
G01N1/2813 (EP);
G01N23/2202 (CN,US);
G01N1/42 (EP,GB,US);
G01N1/28 (GB);
G01N1/312 (GB);
G01N23/2251 (CN,US);
|
| Former IPC [2025/29] | H01J49/06 | Designated contracting states | AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LI, LT, LU, LV, MC, ME, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR [2025/29] | Title | German: | VERFAHREN UND VORRICHTUNG ZUR HERSTELLUNG EINER PROBE EINES ODER MEHRERER MOLEKÜLE ZUR BILDGEBUNG MIT EINEM KRYOELEKTRONENMIKROSKOP | [2025/29] | English: | A METHOD AND APPARATUS OF PREPARING A SAMPLE OF ONE OR MORE MOLECULE(S) FOR IMAGING WITH A CRYO-ELECTRON MICROSCOPE | [2025/29] | French: | PROCÉDÉ ET APPAREIL DE PRÉPARATION D'UN ÉCHANTILLON D'UNE OU PLUSIEURS MOLÉCULES POUR L'IMAGERIE AVEC UN MICROSCOPE CRYO-ÉLECTRONIQUE | [2025/29] | Examination procedure | 09.10.2025 | Amendment by applicant (claims and/or description) | 09.10.2025 | Examination requested [2025/47] | 09.10.2025 | Date on which the examining division has become responsible | 27.02.2026 | Communication of intention to grant the patent | Parent application(s) Tooltip | EP22214294.5 / EP4206643 | Fees paid | Renewal fee | 28.05.2025 | Renewal fee patent year 03 | 10.12.2025 | Renewal fee patent year 04 |
| Opt-out from the exclusive Tooltip competence of the Unified Patent Court | See the Register of the Unified Patent Court for opt-out data | ||
| Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court. | Documents cited: | Search | [DA] US2020411282 (STRAW MARCUS et al.) | [A] GB2598762 (THERMO FISHER SCIENT BREMEN GMBH et al.) | [A] WO2019010436 (WISCONSIN ALUMNI RES FOUND et al.) | [DA] MIKHAILOV VICTOR A. ET AL: "Mass-Selective Soft-Landing of Protein Assemblies with Controlled Landing Energies", ANALYTICAL CHEMISTRY, vol. 86, no. 16, 31 July 2014 (2014-07-31), US, pages 8321 - 8328, XP093044291, ISSN: 0003-2700, DOI: 10.1021/ac5018327 DOI: http://dx.doi.org/10.1021/ac5018327 | [A] ESSER TIM K. ET AL: "Mass-selective and ice-free cryo-EM protein sample preparation via native electrospray ion-beam deposition", BIORXIV, 19 October 2021 (2021-10-19), pages 1 - 29, XP093044445, Retrieved from the Internet DOI: http://dx.doi.org/10.1101/2021.10.18.464782 | [A] ESSER TIM K ET AL: "SUPPLEMENTARY INFORMATION Mass-selective and ice-free cryo-EM protein sample preparation via native electrospray ion-beam deposition", 19 October 2021 (2021-10-19), pages 1 - 9, XP093044434, Retrieved from the Internet | [DA] YIP KA MAN ET AL: "Atomic-resolution protein structure determination by cryo-EM", NATURE, NATURE PUBLISHING GROUP UK, LONDON, vol. 587, no. 7832, 21 October 2020 (2020-10-21), pages 157 - 161, XP037295110, ISSN: 0028-0836, [retrieved on 20201021], DOI: 10.1038/S41586-020-2833-4 DOI: http://dx.doi.org/10.1038/s41586-020-2833-4 | [DA] ROHOU ALEXIS ET AL: "CTFFIND4: Fast and accurate defocus estimation from electron micrographs", JOURNAL OF STRUCTURAL BIOLOGY, ACADEMIC PRESS, UNITED STATES, vol. 192, no. 2, 13 August 2015 (2015-08-13), pages 216 - 221, XP029293557, ISSN: 1047-8477, DOI: 10.1016/J.JSB.2015.08.008 DOI: http://dx.doi.org/10.1016/j.jsb.2015.08.008 | [DA] GUNTUPALLI SAI ROHIT ET AL: "Cryo-Electron Microscopy Structures of Yeast Alcohol Dehydrogenase", BIOCHEMISTRY, vol. 60, no. 9, 23 February 2021 (2021-02-23), pages 663 - 677, XP093044459, ISSN: 0006-2960, DOI: 10.1021/acs.biochem.0c00921 DOI: http://dx.doi.org/10.1021/acs.biochem.0c00921 | [DA] ESSER TIM K ET AL: "Mass-selective and ice-free electron cryomicroscopy protein sample preparation via native electrospray ion-beam deposition", vol. 1, no. 4, 6 August 2022 (2022-08-06), XP093044313, Retrieved from the Internet DOI: http://dx.doi.org/10.1093/pnasnexus/pgac153 | [DA] JEAN-NICOLAS LONGCHAMP ET AL: "Imaging proteins at the truly single molecule level", 1 May 2016 (2016-05-01), XP055303108, Retrieved from the Internet | by applicant | US2020411282 | MIKHAILOV, V. A.MIZE, T. H.BENESCH, J. L. P.ROBINSON, C. V.: "Mass-Selective Soft-Landing of Protein Assemblies with Controlled Landing Energies", ANAL. CHEM., vol. 86, 2014, pages 8321 - 8328, XP093044291, DOI: 10.1021/ac5018327 DOI: http://dx.doi.org/10.1021/ac5018327 | TIM K. ESSER ET AL., MASS-SELECTIVE AND ICE-FREE CRYO-EM PROTEIN SAMPLE PREPARATION VIA NATIVE ELECTROSPRAY ION-BEAM DEPOSITION, Retrieved from the Internet | TIM K. ESSERJAN BHNINGPAUL FREMDLINGMARK T. AGASIDADAM COSTINKYLE FORTALBERT KONIJNENBERGALAN BAHMALEXANDER MAKAROVCAROL V. ROBIN, BIORXIV 2021.10.18.464782, 19 October 2021 (2021-10-19), Retrieved from the Internet | YIP, K. M.FISCHER, N.PAKNIA, E.CHARI, A.STARK, H.: "Atomic-Resolution Protein Structure Determination by Cryo-EM", NATURE, vol. 587, 2020, pages 157 - 161, XP037982520, DOI: 10.1038/s41586-020-2833-4 DOI: http://dx.doi.org/10.1038/s41586-020-2833-4 | ROHOU, A.GRIGORIEFF, N.: "CTFFIND4: Fast and Accurate Defocus Estimation from Electron Micrographs", JOURNAL OF STRUCTURAL BIOLOGY, vol. 192, 2015, pages 216 - 221, XP029293557, DOI: 10.1016/j.jsb.2015.08.008 DOI: http://dx.doi.org/10.1016/j.jsb.2015.08.008 | GUNTUPALLI, S. R.LI, Z.CHANG, L.PLAPP, B. V.SUBRAMANIAN, R.: "Cryo-Electron Microscopy Structures of Yeast Alcohol Dehydrogenase", BIOCHEMISTRY, vol. 60, 2021, pages 663 - 677, XP093044459, DOI: 10.1021/acs.biochem.0c00921 DOI: http://dx.doi.org/10.1021/acs.biochem.0c00921 |