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Extract from the Register of European Patents

EP About this file: EP0679190

EP0679190 - METHOD FOR GENERATING SINGLE-STRANDED DNA MOLECULES [Right-click to bookmark this link]
StatusNo opposition filed within time limit
Status updated on  05.03.2004
Database last updated on 07.10.2024
Most recent event   Tooltip08.11.2013Lapse of the patent in a contracting statepublished on 11.12.2013  [2013/50]
Applicant(s)For all designated states
Beckman Coulter, Inc.
4300 Harbor Boulevard
Fullerton, CA 92834-3100 / US
[2003/19]
Former [2000/28]For all designated states
Orchid BioSciences, Inc.
303 College Road East
Princeton, NJ 08540 / US
Former [2000/26]For all designated states
Orchid BioSciences, Inc.
101 College Road East
Princeton, NJ 08540 / US
Former [2000/21]For all designated states
Orchid Biocomputer, Inc.
303 College Road East
Princeton, NJ 08540 / US
Former [1999/48]For all designated states
Orchid Biocomputer, Inc.
201 Washington Road
Princeton, NJ 08543 / US
Former [1995/44]For all designated states
MOLECULAR TOOL, INC.
Alpha Center, Hopkins Bayview Research Campus, 5210 Eastern Avenue
Baltimore, MD 21224 / US
Inventor(s)01 / NIKIFOROV, Theo
8 Charles Plaza, Apartment 605 NT
Baltimore, MD 21201 / US
02 / KNAPP, Michael, R.
2630 North Calvert
Baltimore, MD 21218 / US
[1995/44]
Representative(s)Bentham, Andrew, et al
J A Kemp
14 South Square
Gray's Inn
London WC1R 5JJ / GB
[N/P]
Former [2003/10]Bentham, Andrew, et al
J.A. Kemp & Co. 14 South Square Gray's Inn
London WC1R 5JJ / GB
Former [1999/48]Goldin, Douglas Michael, et al
J.A. KEMP & CO. 14 South Square Gray's Inn
London WC1R 5LX / GB
Former [1995/44]Goldin, Douglas Michael
J.A. KEMP & CO. 14, South Square Gray's Inn
London WC1R 5LX / GB
Application number, filing date94907855.418.01.1994
[1995/44]
WO1994US00771
Priority number, dateUS1993000506115.01.1993         Original published format: US 5061
US1993015574623.11.1993         Original published format: US 155746
[1995/44]
Filing languageEN
Procedural languageEN
PublicationType: A1 Application with search report
No.:WO9416090
Date:21.07.1994
Language:EN
[1994/17]
Type: A1 Application with search report 
No.:EP0679190
Date:02.11.1995
Language:EN
The application published by WIPO in one of the EPO official languages on 21.07.1994 takes the place of the publication of the European patent application.
[1995/44]
Type: B1 Patent specification 
No.:EP0679190
Date:02.05.2003
Language:EN
[2003/18]
Search report(s)International search report - published on:US21.07.1994
(Supplementary) European search report - dispatched on:EP19.05.1998
ClassificationIPC:C12P19/34
[1995/44]
CPC:
C12Q1/686 (EP,US); C12N15/10 (EP,US); C12Q1/6848 (EP,US);
C12Q1/6853 (EP,US); C12Q1/6858 (EP,US); Y10S435/81 (EP)
C-Set:
C12Q1/6848, C12Q2521/301 (EP,US)
Designated contracting statesAT,   BE,   CH,   DE,   DK,   ES,   FR,   GB,   GR,   IE,   IT,   LI,   LU,   MC,   NL,   PT,   SE [1995/44]
TitleGerman:METHODE ZUM GENERIEREN VON EINZELSTRÄNGIGEN DNA MOLEKÜLEN[1995/44]
English:METHOD FOR GENERATING SINGLE-STRANDED DNA MOLECULES[1995/44]
French:PROCEDE POUR PRODUIRE DES MOLECULES D'ACIDES NUCLEIQUES MONOCATENAIRES[1995/44]
Entry into regional phase17.07.1995National basic fee paid 
17.07.1995Search fee paid 
17.07.1995Designation fee(s) paid 
17.07.1995Examination fee paid 
Examination procedure09.08.1994Request for preliminary examination filed
International Preliminary Examining Authority: US
17.07.1995Examination requested  [1995/44]
17.08.1998Despatch of a communication from the examining division (Time limit: M04)
18.12.1998Reply to a communication from the examining division
01.04.1999Despatch of a communication from the examining division (Time limit: M08)
14.01.2000Despatch of communication that the application is deemed to be withdrawn, reason: reply to the communication from the examining division not received in time
17.03.2000Reply to a communication from the examining division
15.05.2001Despatch of a communication from the examining division (Time limit: M04)
21.09.2001Reply to a communication from the examining division
14.02.2002Despatch of communication of intention to grant (Approval: No)
21.06.2002Despatch of communication of intention to grant (Approval: later approval)
18.11.2002Communication of intention to grant the patent
19.02.2003Fee for grant paid
19.02.2003Fee for publishing/printing paid
Opposition(s)03.02.2004No opposition filed within time limit [2004/17]
Request for further processing for:17.03.2000Request for further processing filed
17.03.2000Full payment received (date of receipt of payment)
Request granted
04.04.2000Decision despatched
Fees paidRenewal fee
26.02.1996Renewal fee patent year 03
26.02.1997Renewal fee patent year 04
08.01.1998Renewal fee patent year 05
11.01.1999Renewal fee patent year 06
05.01.2000Renewal fee patent year 07
04.01.2001Renewal fee patent year 08
25.02.2002Renewal fee patent year 09
20.01.2003Renewal fee patent year 10
Penalty fee
Additional fee for renewal fee
31.01.199603   M06   Fee paid on   26.02.1996
31.01.199704   M06   Fee paid on   26.02.1997
31.01.200209   M06   Fee paid on   25.02.2002
Opt-out from the exclusive  Tooltip
competence of the Unified
Patent Court		 See the Register of the Unified Patent Court for opt-out data
Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Lapses during opposition  TooltipAT02.05.2003
BE02.05.2003
IT02.05.2003
NL02.05.2003
DK02.08.2003
GR02.08.2003
SE02.08.2003
PT04.08.2003
ES13.08.2003
LU18.01.2004
IE19.01.2004
MC31.01.2004
[2013/50]
Former [2008/05]AT02.05.2003
BE02.05.2003
IT02.05.2003
NL02.05.2003
DK02.08.2003
GR02.08.2003
SE02.08.2003
PT04.08.2003
ES13.08.2003
LU18.01.2004
IE19.01.2004
MC31.01.2004
Former [2005/23]AT02.05.2003
BE02.05.2003
NL02.05.2003
DK02.08.2003
GR02.08.2003
SE02.08.2003
PT04.08.2003
ES13.08.2003
LU18.01.2004
IE19.01.2004
MC31.01.2004
Former [2005/03]AT02.05.2003
BE02.05.2003
NL02.05.2003
DK02.08.2003
GR02.08.2003
SE02.08.2003
PT04.08.2003
ES13.08.2003
IE19.01.2004
MC31.01.2004
Former [2005/02]AT02.05.2003
BE02.05.2003
NL02.05.2003
DK02.08.2003
GR02.08.2003
SE02.08.2003
PT04.08.2003
ES13.08.2003
MC31.01.2004
Former [2004/28]AT02.05.2003
BE02.05.2003
NL02.05.2003
DK02.08.2003
GR02.08.2003
SE02.08.2003
PT04.08.2003
ES13.08.2003
Former [2004/23]AT02.05.2003
NL02.05.2003
DK02.08.2003
GR02.08.2003
SE02.08.2003
PT04.08.2003
ES13.08.2003
Former [2004/03]AT02.05.2003
NL02.05.2003
GR02.08.2003
SE02.08.2003
PT04.08.2003
ES13.08.2003
Former [2004/02]NL02.05.2003
GR02.08.2003
SE02.08.2003
PT04.08.2003
Former [2003/50]SE02.08.2003
Documents cited:Search[Y]US4521509  (BENKOVIC STEPHEN J [US], et al) [Y] 1-19 * the whole document *;
 [Y]US4656127  (MUNDY CHRISTOPHER R [GB]) [Y] 12-14 * the whole document *;
 [Y]WO8911548  (CETUS CORP [US]) [Y] 15 * the whole document *;
 [Y]EP0412883  (BERTIN & CIE [FR]) [Y] 12-14 * the whole document *;
 [Y]  - HIGUCHI R G ET AL, "PRODUCTION OF SINGLE-STRANDED DNA TEMPLATES BY EXONUCLEASE DIGESTION FOLLOWING THE POLYMERASE CHAIN REACTION", NUCLEIC ACIDS RESEARCH, (19891011), vol. 17, no. 19, page 5865, XP000226748 [Y] 1-19 * the whole document *
 [PY]  - NEWTON ET AL., "THE PRODUCTION OF PCR PRODUCTS WITH 5' SINGLE-STRANDED TAILS USING PRIMERS THAT INCORPORATE NOVEL PHOSPHORAMIDITE INTERMEDIATES", NUCL. ACIDS RES., (199304), vol. 21, no. 5, pages 1155 - 1162, XP002060902 [PY] 1-19 * the whole document *
 [T]  - NIKIFOROV ET AL., "THE USE OF PHOSPHOROTHIOATE PRIMERS AND EXONUCLEASE HYDROLYSIS FOR THE PREPARATION OF SINGLE-STRANDED PCR PRODUCTS AND THEIR DETECTION BY SOLID-PHASE HYBRIDIZATION", PCR METHODS AND APPLICATIONS, (1994), vol. 3, no. 5, pages 285 - 291, XP002060903 [T] 1-19 * the whole document *
 [T]  - NIKIFOROV T T ET AL, "GENETIC BIT ANALYSIS: A SOLID PHASE METHOD FOR TYPING SINGLE NUCLEOTIDE POLYMORPHISMS", NUCLEIC ACIDS RESEARCH, (199410), vol. 22, no. 20, pages 4167 - 4175, XP002015765 [T] 12-19 * the whole document *
International search[Y]US4683194  (SAIKI RANDALL K [US], et al);
 [Y]US4683195  (MULLIS KARY B [US], et al)
 [Y]  - Nucleic Acids Research, Symposium Series No. 18, issued 1987, GISH and ECKSTEIN, "DNA and RNA Sequencing Utilizing Phosphorothioate Chemistry", pages 253-256, see entire document.
 [Y]  - DNA, Volume 5, No. 2, issued 1986, LABEIT et al., "Laboratory Methods: A New Method of DNA Sequencing Using Deoxynucleoside Alpha-Thiotriphosphates", pages 173-177, see entire document.
 [A]  - Bioconjugate Chemistry, Volume 1, Number 3, issued May/June 1990, GOODCHILD, "Conjugates of Oligonucleotides and Modified Oligonucleotides: A Review of their Synthesis and Properties", pages 165-187, see entire document.
 [A]  - Biochemistry, Volume 15, No. 8, issued 1976, ECKSTEIN and GOODY, "Synthesis and Properties of Diateriosomers of Adenosine 5'(O-1-Thiotriphosphate) and Adenosine 5'-(O-2-Thiotriphosophate)", pages 1685-1691, see entire document.
 [A]  - Proceedings of the National Academy of Sciences, United States of America, Volume 78, Number 12, issued 1981, PUTNEY et al., "A DNA Fragment with an Alpha-Phosphorothioate Nucleotide at One End is Asymmetrically Blocked from Digesion by Exonuclease III and Can Be Replicated In Vivo", pages 7350-7354, see entire
 [A]  - Biochemical and Biophysical Research Communications, Volume 179, Number 3, issued 30 September 1991, KIM et al., "Phosphorothioate Analogues of Oligodeoxyribonucleotide: Synthesis and Activity as Inhibitors of Replication of Human Immunodeficiency Virus", pages 1614-1619, see entire document.
 [A]  - Journal of Organic Chemistry, Volume 54, issued 1989, LUDWIG et al., "Rapid and Efficient Synthesis of Nucleoside 5'-O-(1-Thiotriphosphates),5'-Triphosphates and 2'3'-Cyclophosphorotioates Using 2-Chloro-4H-1,3,2-Benzodioxyphosphorin-4- one", pages 631-635, see entire document.
 [Y]  - Nucleic Acids Research, Volume 13, Number 24, issued 1985, TAYLOR et al., "The Rapid Generation of Oligonucleotide-Directed Mutations at High Frequency Using Phosphorothioate-Modified DNA", pages 8765-8785, see entire document.
 [A]  - Nucleic Acids Research, Volume 16, Number 3, issued 1988, SAYERS et al., "5'-3' Exonucleases in Phosphorothioatebased Oligonucleotide-Directed Mutagenesis", pages 791-802, see entire document.
 [Y]  - Biochemistry, Volume 26, issued 1987, OTT et al., "Protection of Oligonucleotide Primers against Degradation by DNA Polymerase I", pages 8237-8241, see pages 8237, 8239-8241.
 [A]  - Nucleic Acids Research, Volume 14, Number 24, issued 1986, NAKAMAYE et al., "Inhibition of Restriction Endonuclease Nci I Cleavage by Phosphorothioate Groups and its Application of Oligonucleotide-Directed Mutagenesis", pages 9679-9698, see entire document.
The EPO accepts no responsibility for the accuracy of data originating from other authorities; in particular, it does not guarantee that it is complete, up to date or fit for specific purposes.