Extract from the Register of European Patents

EP About this file: EP1100885

EP1100885 - RAPID SUBCLONING USING SITE-SPECIFIC RECOMBINATION [Right-click to bookmark this link]
StatusThe application has been refused
Status updated on  27.04.2007
Database last updated on 11.04.2026
Most recent event   Tooltip18.07.2008Change - representativepublished on 20.08.2008  [2008/34]
Applicant(s)For all designated states
BAYLOR COLLEGE OF MEDICINE
One Baylor Plaza
Houston, TX 77030 / US
[2001/21]
Inventor(s)01 / ELLEDGE, Stephen, J., Dr.
BCMT-T303, Baylor College of Medicine
Houston, TX 77030 / US
02 / LIU, Qinghua
Apt. 26-2G, 2900 Cambridge
Houston, TX 77054 / US
 [2001/31]
Former [2001/21]01 / ELLEDGE, Stephen, J.
2505 Wordsworth Avenue
Houston, TX 77030 / US
02 / LIU, Qinghua
Apt. 26-2G, 2900 Cambridge
Houston, TX 77054 / US
Representative(s)Uexküll & Stolberg
Partnerschaft von
Patent- und Rechtsanwälten mbB
Beselerstrasse 4
22607 Hamburg / DE
[N/P]
Former [2008/34]UEXKÜLL & STOLBERG
Patentanwälte Beselerstrasse 4
22607 Hamburg / DE
Former [2001/21]UEXKÜLL & STOLBERG
Patentanwälte Beselerstrasse 4
22607 Hamburg / DE
Application number, filing date98937141.427.07.1998
[2001/21]
WO1998US15481
Priority number, dateUS1998012238424.07.1998         Original published format: US 122384
[2001/21]
Filing languageEN
Procedural languageEN
PublicationType: A1 Application with search report
No.:WO0005355
Date:03.02.2000
Language:EN
[2000/05]
Type: A1 Application with search report 
No.:EP1100885
Date:23.05.2001
Language:EN
The application published by WIPO in one of the EPO official languages on 03.02.2000 takes the place of the publication of the European patent application.
[2001/21]
Search report(s)International search report - published on:US03.02.2000
(Supplementary) European search report - dispatched on:EP31.10.2001
ClassificationIPC:C12N15/00, C12N15/09, C12N15/63, C12P21/06
[2001/21]
CPC:
C12N15/905 (EP); C12N15/66 (EP)
Designated contracting statesAT,   BE,   CH,   CY,   DE,   DK,   ES,   FI,   FR,   GB,   GR,   IE,   IT,   LI,   LU,   MC,   NL,   PT,   SE [2001/21]
TitleGerman:SCHNELLE SUBKLONIERUNG MITTELS ORTSSPEZIFISCHER REKOMBINATION[2001/21]
English:RAPID SUBCLONING USING SITE-SPECIFIC RECOMBINATION[2001/21]
French:SOUS-CLONAGE RAPIDE FAISANT APPEL A UNE RECOMBINAISON SPECIFIQUE A UN SITE[2001/21]
Entry into regional phase14.02.2001National basic fee paid 
14.02.2001Search fee paid 
14.02.2001Designation fee(s) paid 
14.02.2001Examination fee paid 
Examination procedure09.02.2000Request for preliminary examination filed
International Preliminary Examining Authority: US
14.02.2001Examination requested  [2001/21]
20.04.2001Amendment by applicant (claims and/or description)
30.07.2004Despatch of a communication from the examining division (Time limit: M06)
08.02.2005Reply to a communication from the examining division
03.05.2006Cancellation of oral proceeding that was planned for 10.05.2006
03.05.2006Minutes of oral proceedings despatched
10.05.2006Date of oral proceedings (cancelled)
18.05.2006Despatch of communication that the application is refused, reason: substantive examination [2007/22]
13.04.2007Application refused, date of legal effect [2007/22]
Appeal following examination18.07.2006Appeal received No.  T1780/06
20.04.2007Result of appeal procedure: appeal of the applicant inadmissible
Request for further processing for:08.02.2005Request for further processing filed
08.02.2005Full payment received (date of receipt of payment)
Request granted
24.02.2005Decision despatched
Fees paidRenewal fee
14.02.2001Renewal fee patent year 03
12.07.2001Renewal fee patent year 04
12.07.2002Renewal fee patent year 05
14.07.2003Renewal fee patent year 06
14.07.2004Renewal fee patent year 07
13.07.2005Renewal fee patent year 08
12.07.2006Renewal fee patent year 09
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Documents cited:Search[X]   SAUER B ET AL: "CONSTRUCTION OF ISOGENIC CELL LINES EXPRESSING HUMAN AND RAT ANGIOTENSIN II AT1 RECEPTORS BY CRE-MEDIATED SITE-SPECIFIC RECOMBINATION", METHODS: A COMPANION TO METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC., NEW YORK, NY, US, vol. 4, no. 2, 1992, pages 143 - 149, XP000613888, ISSN: 1046-2023

DOI:   http://dx.doi.org/10.1016/1046-2023(92)90046-B
 [X]   AKAGI K ET AL: "CRE-MEDIATED SOMATIC SITE-SPECIFIC RECOMBINATION IN MICE", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 25, no. 9, 1997, pages 1766 - 1773, XP001004602, ISSN: 0305-1048

DOI:   http://dx.doi.org/10.1093/nar/25.9.1766
 [X]   QIN M ET AL: "CRE RECOMBINASE-MEDIATED SITE-SPECIFIC RECOMBINATION BETWEEN PLANT CHROMOSOMES", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 91, no. 5, 1 March 1994 (1994-03-01), pages 1706 - 1710, XP000615549, ISSN: 0027-8424

DOI:   http://dx.doi.org/10.1073/pnas.91.5.1706
 [X]   ODELL J T ET AL: "SEED-SPECIFIC GENE ACTIVATION MEDIATED BY THE CRE/LOX SITE-SPECIFIC RECOMBINATION SYSTEM", PLANT PHYSIOLOGY, AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD, US, vol. 106, no. 2, 1 October 1994 (1994-10-01), pages 447 - 458, XP002912196, ISSN: 0032-0889

DOI:   http://dx.doi.org/10.1104/pp.106.2.447
 [T]   LIU QINGHUA ET AL: "The univector plasmid-fusion system, a method for rapid construction of recombinant DNA without restriction enzymes.", CURRENT BIOLOGY, vol. 8, no. 24, 3 December 1998 (1998-12-03), pages 1300 - 1309, XP002155212, ISSN: 0960-9822

DOI:   http://dx.doi.org/10.1016/S0960-9822(07)00560-X
 [Y]   HALL RUTH M ET AL: "Mobile gene cassettes and integrons: Capture and spread of genes by site-specific recombination.", MOLECULAR MICROBIOLOGY, vol. 15, no. 4, 1995, pages 593 - 600, XP001028961, ISSN: 0950-382X [Y] 1-31 * the whole document *

DOI:   http://dx.doi.org/10.1111/j.1365-2958.1995.tb02368.x
 [Y]   ELLEDGE S J ET AL: "LAMBDAYES A MULTIFUNCTIONAL COMPLEMENTARY DNA EXPRESSION VECTOR FOR THE ISOLATION OF GENES BY COMPLEMENTATION OF YEAST AND ESCHERICHIA-COLI MUTATIONS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 88, no. 5, 1991, 1991, pages 1731 - 1735, XP002916290, ISSN: 0027-8424

DOI:   http://dx.doi.org/10.1073/pnas.88.5.1731
 [Y]   KILBY N J ET AL: "SITE-SPECIFIC RECOMBINASES: TOOLS FOR GENOME ENGINEERING", TRENDS IN GENETICS, ELSEVIER SCIENCE PUBLISHERS B.V. AMSTERDAM, NL, vol. 9, no. 12, 1993, pages 413 - 421, XP002916291, ISSN: 0168-9525

DOI:   http://dx.doi.org/10.1016/0168-9525(93)90104-P
 [A]   BOUHASSIRA ERIC E ET AL: "Transcriptional behavior of LCR enhancer elements integrated at the same chromosomal locus by recombinase-mediated cassette exchange.", BLOOD, vol. 90, no. 9, 1997, pages 3332 - 3344, XP002143981, ISSN: 0006-4971
 [A]   SCHLAKE THOMAS ET AL: "Use of Mutated FLP Recognition Target (FRT) Sites for the Exchange of Expression Cassettes at Defined Chromosomal Loci.", BIOCHEMISTRY, vol. 33, no. 43, 1994, pages 12746 - 12751, XP000616165, ISSN: 0006-2960 [A] 1-31 * the whole document *

DOI:   http://dx.doi.org/10.1021/bi00209a003
 [Y]   ALADJEM MIRIT I ET AL: "Positive of FLP-mediated unequal sister chromatid exchange products in mammalian cells.", MOLECULAR AND CELLULAR BIOLOGY, vol. 17, no. 2, 1997, pages 857 - 861, XP002180200, ISSN: 0270-7306
 [X]   ANGRAND PIERRE-OLIVIER ET AL: "Inducible expression based on regulated recombination: A single vector strategy for stable expression in cultured cells.", NUCLEIC ACIDS RESEARCH, vol. 26, no. 13, 1 July 1998 (1998-07-01), pages 3263 - 3269, XP001021788, ISSN: 0305-1048 [X] 1-31 * The whole document, see specially Figure 1. *

DOI:   http://dx.doi.org/10.1093/nar/26.13.3263
Examination  AUBRECHT ET AL, GENE, vol. 172, 1996, pages 227 - 231, XP000404274
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