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Extract from the Register of European Patents

EP About this file: EP1604203

EP1604203 - METHODS FOR DIRECT DETECTION OF INDIVIDUAL METHOTREXATE METABOLITES [Right-click to bookmark this link]
StatusNo opposition filed within time limit
Status updated on  03.09.2010
Database last updated on 17.09.2024
Most recent event   Tooltip19.10.2012Lapse of the patent in a contracting state
New state(s): TR		published on 21.11.2012  [2012/47]
Applicant(s)For all designated states
Prometheus Laboratories, Inc.
9410 Carroll Park Drive
San Diego, CA 92121 / US
[N/P]
Former [2009/51]For all designated states
Prometheus Laboratories, Inc.
9410 Caroll Park Drive
San Diego, CA 92121 / US
Former [2005/50]For all designated states
Prometheus Laboratories, Inc.
5739 Pacific Center Boulevard
San Diego, CA 92121-4203 / US
Inventor(s)01 / DERVIEUX, Thierry
4905 Enfield Avenue
Encino, CA 91316 / US
02 / RICHERSON, Russell
5050 East Gleneagles Drive
Tucson, AZ 85718 / US
 [2009/52]
Former [2009/51]01 / DERVIEUX, Thierry
4905 Enfield Avenue
Encino, CA 92121 / US
02 / RICHERSON, Russell
5050 East Gleneagles Drive
Tucson, AZ 85718 / US
Former [2005/50]01 / DERVIEUX, Thierry
3922 Florida Street
San Diego, CA 92104 / US
02 / RICHERSON, Russell
5050 East Gleneagles Drive
Tucson, AZ 85718 / US
Representative(s)Uexküll & Stolberg
Partnerschaft von
Patent- und Rechtsanwälten mbB
Beselerstraße 4
22607 Hamburg / DE
[N/P]
Former [2008/34]UEXKÜLL & STOLBERG
Patentanwälte Beselerstrasse 4
22607 Hamburg / DE
Former [2005/50]UEXKÜLL & STOLBERG
Patentanwälte Beselerstrasse 4
22607 Hamburg / DE
Application number, filing date04716493.402.03.2004
[2005/50]
WO2004US06312
Priority number, dateUS2003038406607.03.2003         Original published format: US 384066
[2005/50]
Filing languageEN
Procedural languageEN
PublicationType: A2 Application without search report
No.:WO2004081521
Date:23.09.2004
Language:EN
[2004/39]
Type: A2 Application without search report 
No.:EP1604203
Date:14.12.2005
Language:EN
The application published by WIPO in one of the EPO official languages on 23.09.2004 takes the place of the publication of the European patent application.
[2005/50]
Type: B1 Patent specification 
No.:EP1604203
Date:28.10.2009
Language:EN
[2009/44]
Search report(s)International search report - published on:US02.12.2004
(Supplementary) European search report - dispatched on:EP13.06.2007
ClassificationIPC:G01N33/48, G01N33/00, G01N30/02, G01N21/75, G01N21/62, A01N1/02, C12Q1/00, C12Q1/02
[2005/50]
CPC:
G01N33/94 (EP,US); A61P17/06 (EP); A61P19/02 (EP);
A61P29/00 (EP); A61P35/00 (EP); A61P37/02 (EP);
G01N2800/52 (EP,US); Y10T436/17 (EP,US); Y10T436/24 (EP,US) (-)
Designated contracting statesAT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HU,   IE,   IT,   LI,   LU,   MC,   NL,   PL,   PT,   RO,   SE,   SI,   SK,   TR [2005/50]
Extension statesALNot yet paid
LTNot yet paid
LVNot yet paid
MKNot yet paid
TitleGerman:VERFAHREN ZUM DIREKTEN NACHWEIS EINZELNER METHOTREXATMETABOLITE[2005/50]
English:METHODS FOR DIRECT DETECTION OF INDIVIDUAL METHOTREXATE METABOLITES[2005/50]
French:METHODES DE DETECTION DIRECTE DE METABOLITES DE METHOTREXATE INDIVIDUELS[2005/50]
Entry into regional phase26.09.2005National basic fee paid 
26.09.2005Search fee paid 
26.09.2005Designation fee(s) paid 
26.09.2005Examination fee paid 
Examination procedure07.10.2004Request for preliminary examination filed
International Preliminary Examining Authority: US
26.09.2005Amendment by applicant (claims and/or description)
26.09.2005Examination requested  [2005/50]
06.11.2007Despatch of a communication from the examining division (Time limit: M04)
03.03.2008Reply to a communication from the examining division
25.03.2008Despatch of a communication from the examining division (Time limit: M04)
21.07.2008Reply to a communication from the examining division
13.01.2009Date of oral proceedings
06.02.2009Minutes of oral proceedings despatched
20.04.2009Communication of intention to grant the patent
20.08.2009Fee for grant paid
20.08.2009Fee for publishing/printing paid
Divisional application(s)EP09013484.2  / EP2148199
Opposition(s)29.07.2010No opposition filed within time limit [2010/40]
Fees paidRenewal fee
29.03.2006Renewal fee patent year 03
28.03.2007Renewal fee patent year 04
27.03.2008Renewal fee patent year 05
13.03.2009Renewal fee patent year 06
Opt-out from the exclusive  Tooltip
competence of the Unified
Patent Court		 See the Register of the Unified Patent Court for opt-out data
Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Lapses during opposition  TooltipAT28.10.2009
CY28.10.2009
CZ28.10.2009
EE28.10.2009
PL28.10.2009
RO28.10.2009
SI28.10.2009
SK28.10.2009
TR28.10.2009
BG28.01.2010
GR29.01.2010
PT01.03.2010
IE02.03.2010
MC31.03.2010
HU29.04.2010
[2012/47]
Former [2012/42]AT28.10.2009
CY28.10.2009
CZ28.10.2009
EE28.10.2009
PL28.10.2009
RO28.10.2009
SI28.10.2009
SK28.10.2009
BG28.01.2010
GR29.01.2010
PT01.03.2010
IE02.03.2010
MC31.03.2010
HU29.04.2010
Former [2011/07]AT28.10.2009
CY28.10.2009
CZ28.10.2009
EE28.10.2009
PL28.10.2009
RO28.10.2009
SI28.10.2009
SK28.10.2009
BG28.01.2010
GR29.01.2010
PT01.03.2010
IE02.03.2010
MC31.03.2010
Former [2010/46]AT28.10.2009
CY28.10.2009
CZ28.10.2009
EE28.10.2009
PL28.10.2009
RO28.10.2009
SI28.10.2009
SK28.10.2009
BG28.01.2010
GR29.01.2010
PT01.03.2010
MC31.03.2010
Former [2010/39]AT28.10.2009
CY28.10.2009
CZ28.10.2009
EE28.10.2009
PL28.10.2009
RO28.10.2009
SI28.10.2009
SK28.10.2009
BG28.01.2010
PT01.03.2010
Former [2010/36]AT28.10.2009
CY28.10.2009
CZ28.10.2009
EE28.10.2009
PL28.10.2009
RO28.10.2009
SI28.10.2009
BG28.01.2010
PT01.03.2010
Former [2010/35]AT28.10.2009
CY28.10.2009
EE28.10.2009
PL28.10.2009
RO28.10.2009
SI28.10.2009
BG28.01.2010
PT01.03.2010
Former [2010/30]AT28.10.2009
CY28.10.2009
PL28.10.2009
SI28.10.2009
PT01.03.2010
Former [2010/25]CY28.10.2009
PL28.10.2009
SI28.10.2009
PT01.03.2010
Former [2010/23]PL28.10.2009
SI28.10.2009
PT01.03.2010
Former [2010/22]PT01.03.2010
Documents cited:Search[E]WO2004020622  (PROMETHEUS LAB INC [US], et al) [E] 1-44 * example 1 *;
 [X]  - BROUWER CONNIE ET AL, "Analysis of methotrexate (MTX) and MTX-polyglutamates using affinity/HPLC with post-column derivatization", FASEB JOURNAL, & ANNUAL MEETING OF PROFESSIONAL RESEARCH SCIENTISTS: EXPERIMENTAL BIOLOGY 2000; SAN DIEGO, CALIFORNIA, USA; APRIL 15-18, 2000, (20000315), vol. 14, no. 4, ISSN 0892-6638, page A720, XP008078811 [X] 1-44 * abstract *
International search[X]  - MASSON E. ET AL, "Accumulation of Methotrexate Polyglutamates in Lymphoblasts Is a Determinant of Antileukemic Effects In Vivo . A Rationale for High-Dose Methotrexate", JOURNAL OF CLINICAL INVESTIGATION, (199601), vol. 97, no. 1, pages 73 - 80, XP002982097

DOI:   http://dx.doi.org/10.1172/JCI118409
 [X]  - GALPIN A.J. ET AL, "Differences in folylpolyglutamate synthetase and dihydrofolate reductase expression in human B-lineage versus T-lineage leukemic lymphoblasts: mechanismsfor lineage differences in methotrexate polyglutamylation and cytotoxicity.", MOLECULAR PHARMACOLOGY, (1997), vol. 52, pages 155 - 163, XP002982098
 [X]  - KRAKOWER G.R. AND NYLEN P.A. AND KAMEN B.A., "Separation and identification of subpicomole amounts of methotrexate polyglutamates in animal and human biopsy material", ANALYTICAL BIOCHEMISTRY, (1982), vol. 122, no. 2, pages 412 - 416, XP002903650

DOI:   http://dx.doi.org/10.1016/0003-2697(82)90303-7
 [Y]  - RUBINO F.M., "Separation methods for methotrexate, its structural analogues and metabolites", JOURNAL OF CHROMATOGRAPHY, vol. 764, pages 217 - 254, XP004322155

DOI:   http://dx.doi.org/10.1016/S0378-4347(01)00402-9
 [Y]  - SALAMOUN J. AND FRANTISEK J., "Determination of methotrexate and its metabolites 7-hydroxymethotrexate and 2,4-diamino-N10-methylpteroic acid in biological fluids by liquid chromatography with fluorimetric detection.", JOURNAL OF CHROMATOGRAPHY, (1986), vol. 378, no. 1, pages 173 - 181, XP002903614
 [Y]  - SALAMOUN J. ET AL, "Column liquid chromatography of methotrexate and its metabolites using a post-column photochemical reactor and fluorescence detection", JOURNAL OF CHROMATOGRAPHY, (1987), vol. 419, pages 2137 - 223, XP002971635

DOI:   http://dx.doi.org/10.1016/0378-4347(87)80279-7
 [A]  - DERVIEUX T. ET AL, "Effect of methotrexate polyglutamates on thioguanine nucleotide concentrations during continuation therapy of acute lymphoblastic leukemia with mercaptopurine", LEUKEMIA, (2002), vol. 16, pages 209 - 212, XP002982099

DOI:   http://dx.doi.org/10.1038/sj.leu.2402373
 [A]  - SYNOLD T.W. ET AL, "Blast cell methotrexate-polyglutamate accumulation in vivo differs by lineage, ploidy, and methotrexate dose in acute lymphoblastic leukemia.", JOURNAL OF CLINICAL INVESTIGATION, (199411), vol. 94, pages 1996 - 2001, XP002982100

DOI:   http://dx.doi.org/10.1172/JCI117552
 [A]  - DERVIEUX T. ET AL, "HPLC Determination of Erythrocyte Methotrexate Polyglutamates after Low-Dose Methotrexate Therapy in Patients with Rheumatoid Arthritis", CLINICAL CHEMISTRY, (2003), vol. 10, pages 1632 - 1641, XP002903601

DOI:   http://dx.doi.org/10.1373/49.10.1632
Examination   - FREIDOUN ALBERTIONI ET AL, "Simultaneous quantitation of methotrexate and its two main metabolites in biological fluids by a novel solid-phase extraction procedure using high-performance liquid chromatography", JOURNAL OF CHROMATOGRAPHY B, (1995), vol. 665, pages 163 - 170, XP004043641

DOI:   http://dx.doi.org/10.1016/0378-4347(94)00507-2
The EPO accepts no responsibility for the accuracy of data originating from other authorities; in particular, it does not guarantee that it is complete, up to date or fit for specific purposes.