| EP2142654 - HIGH EFFICIENCY ELECTROPORATION BUFFER [Right-click to bookmark this link] | Status | The application is deemed to be withdrawn Status updated on 23.03.2012 Database last updated on 18.03.2026 | Most recent event Tooltip | 23.03.2012 | Application deemed to be withdrawn | published on 25.04.2012 [2012/17] | Applicant(s) | For all designated states Bio-Rad Laboratories, Inc. 1000 Alfred Nobel Drive Hercules, CA 94547 / US | [2010/02] | Inventor(s) | 01 /
RUBIO, Teresa 707 Pomona Avenue El Cerrito, California 94530 / US | 02 /
TEREFE, Joseph 7301 Elverta Rail Way Rio Linda, California 95673 / US | [2010/02] | Representative(s) | dompatent Partnerschaft von Patentanwälten und Rechtsanwälten mbB Deichmannhaus am Dom Bahnhofsvorplatz 1 50667 Köln / DE | [N/P] |
| Former [2010/15] | von Kreisler Selting Werner Deichmannhaus am Dom Bahnhofsvorplatz 1 50667 Köln / DE | ||
| Former [2010/02] | Helbing, Jörg Patentanwälte von Kreisler Selting Werner Bahnhofsvorplatz 1 Deichmannhaus am Dom 50667 Köln / DE | Application number, filing date | 08733134.4 | 04.04.2008 | [2010/02] | WO2008US59457 | Priority number, date | US20070742245 | 30.04.2007 Original published format: US 742245 | [2010/02] | Filing language | EN | Procedural language | EN | Publication | Type: | A1 Application with search report | No.: | WO2008134200 | Date: | 06.11.2008 | Language: | EN | [2008/45] | Type: | A1 Application with search report | No.: | EP2142654 | Date: | 13.01.2010 | Language: | EN | The application published by WIPO in one of the EPO official languages on 06.11.2008 takes the place of the publication of the European patent application. | [2010/02] | Search report(s) | International search report - published on: | US | 06.11.2008 | (Supplementary) European search report - dispatched on: | EP | 30.03.2010 | Classification | IPC: | C12N15/87, C12N13/00, C12N5/00 | [2010/02] | CPC: |
C12N15/87 (EP,US)
| Designated contracting states | AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LI, LT, LU, LV, MC, MT, NL, NO, PL, PT, RO, SE, SI, SK, TR [2010/02] | Title | German: | HOCHWIRKSAMER ELEKTROPORATIONSPUFFER | [2010/02] | English: | HIGH EFFICIENCY ELECTROPORATION BUFFER | [2010/02] | French: | TAMPON D'ÉLECTROPORATION À HAUTE EFFICACITÉ | [2010/02] | Entry into regional phase | 27.10.2009 | National basic fee paid | 27.10.2009 | Search fee paid | 27.10.2009 | Designation fee(s) paid | 27.10.2009 | Examination fee paid | Examination procedure | 27.10.2009 | Examination requested [2010/02] | 25.06.2010 | Amendment by applicant (claims and/or description) | 01.11.2011 | Application deemed to be withdrawn, date of legal effect [2012/17] | 09.12.2011 | Despatch of communication that the application is deemed to be withdrawn, reason: renewal fee not paid in time [2012/17] | Fees paid | Renewal fee | 26.04.2010 | Renewal fee patent year 03 | Penalty fee | Additional fee for renewal fee | 30.04.2011 | 04   M06   Not yet paid |
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| Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court. | Documents cited: | Search | [YD] MELKONYAN H ET AL: "Electroporation efficiency in mammalian cells is increased by dimethyl sulfoxide (DMSO).", NUCLEIC ACIDS RESEARCH 1 NOV 1996, vol. 24, no. 21, 1 November 1996 (1996-11-01), pages 4356 - 4357, XP002573287, ISSN: 0305-1048 [YD] 1-12 * the whole document * DOI: http://dx.doi.org/10.1093/nar/24.21.4356 | [Y] BRIGHT GARY R ET AL: "Delivery of macromolecules into adherent cells via electroporation for use in fluorescence spectroscopic imaging and metabolic studies", CYTOMETRY, vol. 24, no. 3, 1996, pages 226 - 233, XP002573288, ISSN: 0196-4763 [Y] 1-12 * the whole document * | [YD] GOLZIO MURIEL ET AL: "Control by osmotic pressure of voltage-induced permeabilization and gene transfer in mammalian cells", BIOPHYSICAL JOURNAL, vol. 74, no. 6, June 1998 (1998-06-01), pages 3015 - 3022, XP002573289, ISSN: 0006-3495 [YD] 1-12 * the whole document * * page 3016, column 1, paragraph 5 * DOI: http://dx.doi.org/10.1016/S0006-3495(98)78009-9 | [Y] DUENAS SANTIAGO ET AL: "Electroporation of Neisseria meningitidis with plasmid DNA", BIOTECNOLOGIA APLICADA, LA HABANA, CU, vol. 15, no. 4, 1 October 1998 (1998-10-01), pages 247 - 249, XP009130908, ISSN: 1027-2852 [Y] 1-12 * the whole document * | [Y] BHOWMIK T ET AL: "DEVELOPMENT OF AN ELECTROPORATION PROCEDURE FOR GENE DISRUPTION IN LACTOBACILLUS HELVETICUS CNRZ 32", JOURNAL OF GENERAL MICROBIOLOGY, SOCIETY FOR MICROBIOLOGY, READING, GB, vol. 139, no. 7, 1 January 1993 (1993-01-01), pages 1433 - 1439, XP001205842, ISSN: 0022-1287 [Y] 1-12 * the whole document * | [YD] MUSSAUER HEIKO ET AL: "Trehalose improves survival of electrotransfected mammalian cells", CYTOMETRY, vol. 45, no. 3, 1 November 2001 (2001-11-01), pages 161 - 169, XP002573290, ISSN: 0196-4763 [YD] 1-12 * the whole document * | [AD] VAN DEN HOFF M J ET AL: "Electroporation in 'intracellular' buffer increases cell survival", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 20, no. 11, 11 June 1992 (1992-06-11), pages 2902, XP002120723, ISSN: 0305-1048 [AD] 1-12 * the whole document * | International search | [Y] BHANDAL I.S. ET AL.: "Trehalose as Cryoprotectant for the Freeze Preservation of Carrot and Tobacco Cells", PLANT PHYSIOL., vol. 78, no. 2, June 1985 (1985-06-01), pages 430 - 432, XP001156094 [Y] |