Extract from the Register of European Patents

EP About this file: EP2142654

EP2142654 - HIGH EFFICIENCY ELECTROPORATION BUFFER [Right-click to bookmark this link]
StatusThe application is deemed to be withdrawn
Status updated on  23.03.2012
Database last updated on 18.03.2026
Most recent event   Tooltip23.03.2012Application deemed to be withdrawnpublished on 25.04.2012  [2012/17]
Applicant(s)For all designated states
Bio-Rad Laboratories, Inc.
1000 Alfred Nobel Drive
Hercules, CA 94547 / US
[2010/02]
Inventor(s)01 / RUBIO, Teresa
707 Pomona Avenue
El Cerrito, California 94530 / US
02 / TEREFE, Joseph
7301 Elverta Rail Way
Rio Linda, California 95673 / US
 [2010/02]
Representative(s)dompatent
Partnerschaft von
Patentanwälten und Rechtsanwälten mbB
Deichmannhaus am Dom
Bahnhofsvorplatz 1
50667 Köln / DE
[N/P]
Former [2010/15]von Kreisler Selting Werner
Deichmannhaus am Dom Bahnhofsvorplatz 1
50667 Köln / DE
Former [2010/02]Helbing, Jörg
Patentanwälte von Kreisler Selting Werner Bahnhofsvorplatz 1 Deichmannhaus am Dom
50667 Köln / DE
Application number, filing date08733134.404.04.2008
[2010/02]
WO2008US59457
Priority number, dateUS2007074224530.04.2007         Original published format: US 742245
[2010/02]
Filing languageEN
Procedural languageEN
PublicationType: A1 Application with search report
No.:WO2008134200
Date:06.11.2008
Language:EN
[2008/45]
Type: A1 Application with search report 
No.:EP2142654
Date:13.01.2010
Language:EN
The application published by WIPO in one of the EPO official languages on 06.11.2008 takes the place of the publication of the European patent application.
[2010/02]
Search report(s)International search report - published on:US06.11.2008
(Supplementary) European search report - dispatched on:EP30.03.2010
ClassificationIPC:C12N15/87, C12N13/00, C12N5/00
[2010/02]
CPC:
C12N15/87 (EP,US)
Designated contracting statesAT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   LV,   MC,   MT,   NL,   NO,   PL,   PT,   RO,   SE,   SI,   SK,   TR [2010/02]
TitleGerman:HOCHWIRKSAMER ELEKTROPORATIONSPUFFER[2010/02]
English:HIGH EFFICIENCY ELECTROPORATION BUFFER[2010/02]
French:TAMPON D'ÉLECTROPORATION À HAUTE EFFICACITÉ[2010/02]
Entry into regional phase27.10.2009National basic fee paid 
27.10.2009Search fee paid 
27.10.2009Designation fee(s) paid 
27.10.2009Examination fee paid 
Examination procedure27.10.2009Examination requested  [2010/02]
25.06.2010Amendment by applicant (claims and/or description)
01.11.2011Application deemed to be withdrawn, date of legal effect  [2012/17]
09.12.2011Despatch of communication that the application is deemed to be withdrawn, reason: renewal fee not paid in time  [2012/17]
Fees paidRenewal fee
26.04.2010Renewal fee patent year 03
Penalty fee
Additional fee for renewal fee
30.04.201104   M06   Not yet paid
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Documents cited:Search[YD]   MELKONYAN H ET AL: "Electroporation efficiency in mammalian cells is increased by dimethyl sulfoxide (DMSO).", NUCLEIC ACIDS RESEARCH 1 NOV 1996, vol. 24, no. 21, 1 November 1996 (1996-11-01), pages 4356 - 4357, XP002573287, ISSN: 0305-1048 [YD] 1-12 * the whole document *

DOI:   http://dx.doi.org/10.1093/nar/24.21.4356
 [Y]   BRIGHT GARY R ET AL: "Delivery of macromolecules into adherent cells via electroporation for use in fluorescence spectroscopic imaging and metabolic studies", CYTOMETRY, vol. 24, no. 3, 1996, pages 226 - 233, XP002573288, ISSN: 0196-4763 [Y] 1-12 * the whole document *
 [YD]   GOLZIO MURIEL ET AL: "Control by osmotic pressure of voltage-induced permeabilization and gene transfer in mammalian cells", BIOPHYSICAL JOURNAL, vol. 74, no. 6, June 1998 (1998-06-01), pages 3015 - 3022, XP002573289, ISSN: 0006-3495 [YD] 1-12 * the whole document * * page 3016, column 1, paragraph 5 *

DOI:   http://dx.doi.org/10.1016/S0006-3495(98)78009-9
 [Y]   DUENAS SANTIAGO ET AL: "Electroporation of Neisseria meningitidis with plasmid DNA", BIOTECNOLOGIA APLICADA, LA HABANA, CU, vol. 15, no. 4, 1 October 1998 (1998-10-01), pages 247 - 249, XP009130908, ISSN: 1027-2852 [Y] 1-12 * the whole document *
 [Y]   BHOWMIK T ET AL: "DEVELOPMENT OF AN ELECTROPORATION PROCEDURE FOR GENE DISRUPTION IN LACTOBACILLUS HELVETICUS CNRZ 32", JOURNAL OF GENERAL MICROBIOLOGY, SOCIETY FOR MICROBIOLOGY, READING, GB, vol. 139, no. 7, 1 January 1993 (1993-01-01), pages 1433 - 1439, XP001205842, ISSN: 0022-1287 [Y] 1-12 * the whole document *
 [YD]   MUSSAUER HEIKO ET AL: "Trehalose improves survival of electrotransfected mammalian cells", CYTOMETRY, vol. 45, no. 3, 1 November 2001 (2001-11-01), pages 161 - 169, XP002573290, ISSN: 0196-4763 [YD] 1-12 * the whole document *
 [AD]   VAN DEN HOFF M J ET AL: "Electroporation in 'intracellular' buffer increases cell survival", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 20, no. 11, 11 June 1992 (1992-06-11), pages 2902, XP002120723, ISSN: 0305-1048 [AD] 1-12 * the whole document *
International search[Y]   BHANDAL I.S. ET AL.: "Trehalose as Cryoprotectant for the Freeze Preservation of Carrot and Tobacco Cells", PLANT PHYSIOL., vol. 78, no. 2, June 1985 (1985-06-01), pages 430 - 432, XP001156094 [Y]
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