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Extract from the Register of European Patents

EP About this file: EP2220232

EP2220232 - SUCCINIC ACID PRODUCTION IN A EUKARYOTIC CELL [Right-click to bookmark this link]
StatusNo opposition filed within time limit
Status updated on  10.07.2015
Database last updated on 14.09.2024
Most recent event   Tooltip15.07.2016Lapse of the patent in a contracting state
New state(s): MT		published on 17.08.2016  [2016/33]
Applicant(s)For all designated states
DSM IP Assets B.V.
Het Overloon 1
6411 TE Heerlen / NL
[2010/34]
Former [2009/38]For all designated states
DSM IP Assets B.V.
Het Overloon 1
6411 TE Heerlen / NL
Inventor(s)01 / VERWAAL, René
Marsdiep 7
NL-2631 NL Nootdorp / NL
02 / WU, Liang
Van Barenstraat 25
NL-2628 BC Delft / NL
03 / DAMVELD, Robbertus, Antonius
Slufterhof 17
NL-2651 NK Berkel en Rodenrijs / NL
04 / SAGT, Cornelis, Maria, Jacobus
Karl Millockerhof 19
NL-3543 HV Utrecht / NL
 [2010/40]
Former [2010/34]01 / VERWAAL, René
Marsdiep 7
NL-2631 NL Nootdorp / NL
02 / WU, Liang
Sweelinckstraat 36
NL-2625 VK Delft / NL
03 / DAMVELD, Robbertus, Antonius
Slufterhof 17
NL-2651 NK Berkel en Rodenrijs / NL
04 / SAGT, Cornelis, Maria, Jacobus
Karl Millockerhof 19
NL-3543 HV Utrecht / NL
Representative(s)Cazemier, Anne Engeline, et al
DSM Intellectual Property
P.O. Box 4
6100 AA Echt / NL
[2014/36]
Former [2011/11]Cazemier, Anne Engeline, et al
DSM Intellectual Property P.O. Box 130
6100 AC Echt / NL
Former [2010/34]Cazemier, Anne Engeline, et al
DSM Intellectual Property Delft Office (600-0240) P.O. Box 1
2600 MA Delft / NL
Application number, filing date08851178.714.11.2008
[2010/34]
WO2008EP65583
Priority number, dateEP2007012111720.11.2007         Original published format: EP 07121117
EP2007012112020.11.2007         Original published format: EP 07121120
EP2007012111320.11.2007         Original published format: EP 07121113
EP2008015696127.05.2008         Original published format: EP 08156961
EP2008015696027.05.2008         Original published format: EP 08156960
EP2008015695927.05.2008         Original published format: EP 08156959
[2010/34]
Filing languageEN
Procedural languageEN
PublicationType: A1 Application with search report
No.:WO2009065778
Date:28.05.2009
Language:EN
[2009/22]
Type: A1 Application with search report 
No.:EP2220232
Date:25.08.2010
Language:EN
The application published by WIPO in one of the EPO official languages on 28.05.2009 takes the place of the publication of the European patent application.
[2010/34]
Type: B1 Patent specification 
No.:EP2220232
Date:03.09.2014
Language:EN
[2014/36]
Search report(s)International search report - published on:EP28.05.2009
ClassificationIPC:C12N15/53, C12N9/02, C12P7/46, C12N1/15, C12N1/19
[2013/12]
CPC:
C12P7/46 (EP,EA,US); C12N9/001 (EP,EA,US); C12N15/81 (EA,US);
C12N15/815 (EA,US); C12N9/88 (EP,EA,US); Y02P20/52 (EA,US)
Former IPC [2010/34]C12N15/53, C12N9/02, C12P7/46
Designated contracting statesAT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   LV,   MC,   MT,   NL,   NO,   PL,   PT,   RO,   SE,   SI,   SK,   TR [2010/34]
TitleGerman:BERNSTEINSÄUREPRODUKTION IN EINER EUKARYONTISCHEN ZELLE[2010/34]
English:SUCCINIC ACID PRODUCTION IN A EUKARYOTIC CELL[2010/34]
French:PRODUCTION D'ACIDE SUCCINIQUE DANS UNE CELLULE EUCARYOTE[2010/34]
Entry into regional phase17.06.2010National basic fee paid 
17.06.2010Designation fee(s) paid 
17.06.2010Examination fee paid 
Examination procedure07.09.2009Request for preliminary examination filed
International Preliminary Examining Authority: EP
17.06.2010Examination requested  [2010/34]
16.07.2010Amendment by applicant (claims and/or description)
20.01.2011Despatch of a communication from the examining division (Time limit: M04)
05.05.2011Reply to a communication from the examining division
25.05.2011Despatch of a communication from the examining division (Time limit: M04)
28.06.2011Reply to a communication from the examining division
01.10.2012Observations by third parties
08.01.2014Communication of intention to grant the patent
19.05.2014Disapproval of the communication of intention to grant the patent by the applicant or resumption of examination proceedings by the EPO
06.06.2014Communication of intention to grant the patent
24.07.2014Fee for grant paid
24.07.2014Fee for publishing/printing paid
24.07.2014Receipt of the translation of the claim(s)
Divisional application(s)EP13170415.7  / EP2687602
The date of the Examining Division's first communication in respect of the earliest application for which a communication has been issued is  20.01.2011
Opposition(s)04.06.2015No opposition filed within time limit [2015/33]
Fees paidRenewal fee
24.11.2010Renewal fee patent year 03
23.11.2011Renewal fee patent year 04
13.11.2012Renewal fee patent year 05
18.11.2013Renewal fee patent year 06
Opt-out from the exclusive  Tooltip
competence of the Unified
Patent Court		 See the Register of the Unified Patent Court for opt-out data
Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Lapses during opposition  TooltipHU14.11.2008
BE03.09.2014
BG03.09.2014
CY03.09.2014
CZ03.09.2014
DK03.09.2014
EE03.09.2014
FI03.09.2014
HR03.09.2014
LT03.09.2014
LV03.09.2014
MC03.09.2014
MT03.09.2014
PL03.09.2014
RO03.09.2014
SE03.09.2014
SI03.09.2014
SK03.09.2014
TR03.09.2014
IE14.11.2014
LU14.11.2014
CH30.11.2014
LI30.11.2014
NO03.12.2014
GR04.12.2014
IS03.01.2015
PT05.01.2015
[2016/33]
Former [2016/32]HU14.11.2008
BE03.09.2014
BG03.09.2014
CY03.09.2014
CZ03.09.2014
DK03.09.2014
EE03.09.2014
FI03.09.2014
HR03.09.2014
LT03.09.2014
LV03.09.2014
MC03.09.2014
PL03.09.2014
RO03.09.2014
SE03.09.2014
SI03.09.2014
SK03.09.2014
TR03.09.2014
IE14.11.2014
LU14.11.2014
CH30.11.2014
LI30.11.2014
NO03.12.2014
GR04.12.2014
IS03.01.2015
PT05.01.2015
Former [2016/25]BG03.09.2014
CY03.09.2014
CZ03.09.2014
DK03.09.2014
EE03.09.2014
FI03.09.2014
HR03.09.2014
LT03.09.2014
LV03.09.2014
MC03.09.2014
PL03.09.2014
RO03.09.2014
SE03.09.2014
SI03.09.2014
SK03.09.2014
IE14.11.2014
LU14.11.2014
BE30.11.2014
CH30.11.2014
LI30.11.2014
NO03.12.2014
GR04.12.2014
IS03.01.2015
PT05.01.2015
Former [2015/50]CY03.09.2014
CZ03.09.2014
DK03.09.2014
EE03.09.2014
FI03.09.2014
HR03.09.2014
LT03.09.2014
LV03.09.2014
MC03.09.2014
PL03.09.2014
RO03.09.2014
SE03.09.2014
SI03.09.2014
SK03.09.2014
IE14.11.2014
LU14.11.2014
BE30.11.2014
CH30.11.2014
LI30.11.2014
NO03.12.2014
GR04.12.2014
IS03.01.2015
PT05.01.2015
Former [2015/46]CY03.09.2014
CZ03.09.2014
DK03.09.2014
EE03.09.2014
FI03.09.2014
HR03.09.2014
LT03.09.2014
LV03.09.2014
MC03.09.2014
PL03.09.2014
RO03.09.2014
SE03.09.2014
SK03.09.2014
IE14.11.2014
LU14.11.2014
BE30.11.2014
CH30.11.2014
LI30.11.2014
NO03.12.2014
GR04.12.2014
IS03.01.2015
PT05.01.2015
Former [2015/34]CY03.09.2014
CZ03.09.2014
DK03.09.2014
EE03.09.2014
FI03.09.2014
HR03.09.2014
LT03.09.2014
LV03.09.2014
MC03.09.2014
PL03.09.2014
RO03.09.2014
SE03.09.2014
SK03.09.2014
LU14.11.2014
BE30.11.2014
CH30.11.2014
LI30.11.2014
NO03.12.2014
GR04.12.2014
IS03.01.2015
PT05.01.2015
Former [2015/33]CY03.09.2014
CZ03.09.2014
EE03.09.2014
FI03.09.2014
HR03.09.2014
LT03.09.2014
LV03.09.2014
MC03.09.2014
PL03.09.2014
RO03.09.2014
SE03.09.2014
SK03.09.2014
LU14.11.2014
BE30.11.2014
CH30.11.2014
LI30.11.2014
NO03.12.2014
GR04.12.2014
IS03.01.2015
PT05.01.2015
Former [2015/32]CY03.09.2014
CZ03.09.2014
EE03.09.2014
FI03.09.2014
HR03.09.2014
LT03.09.2014
LV03.09.2014
MC03.09.2014
PL03.09.2014
RO03.09.2014
SE03.09.2014
SK03.09.2014
LU14.11.2014
BE30.11.2014
NO03.12.2014
GR04.12.2014
IS03.01.2015
PT05.01.2015
Former [2015/30]CY03.09.2014
CZ03.09.2014
EE03.09.2014
FI03.09.2014
HR03.09.2014
LT03.09.2014
LV03.09.2014
PL03.09.2014
RO03.09.2014
SE03.09.2014
SK03.09.2014
LU14.11.2014
BE30.11.2014
NO03.12.2014
GR04.12.2014
IS03.01.2015
PT05.01.2015
Former [2015/24]CY03.09.2014
CZ03.09.2014
EE03.09.2014
FI03.09.2014
HR03.09.2014
LT03.09.2014
LV03.09.2014
PL03.09.2014
RO03.09.2014
SE03.09.2014
SK03.09.2014
NO03.12.2014
GR04.12.2014
IS03.01.2015
PT05.01.2015
Former [2015/23]CY03.09.2014
CZ03.09.2014
EE03.09.2014
FI03.09.2014
HR03.09.2014
LT03.09.2014
LV03.09.2014
RO03.09.2014
SE03.09.2014
SK03.09.2014
NO03.12.2014
GR04.12.2014
IS03.01.2015
PT05.01.2015
Former [2015/22]CY03.09.2014
FI03.09.2014
HR03.09.2014
LT03.09.2014
LV03.09.2014
RO03.09.2014
SE03.09.2014
NO03.12.2014
GR04.12.2014
PT05.01.2015
Former [2015/12]CY03.09.2014
FI03.09.2014
HR03.09.2014
LT03.09.2014
LV03.09.2014
SE03.09.2014
NO03.12.2014
GR04.12.2014
Former [2015/11]CY03.09.2014
FI03.09.2014
LT03.09.2014
SE03.09.2014
NO03.12.2014
GR04.12.2014
Former [2015/10]FI03.09.2014
LT03.09.2014
SE03.09.2014
NO03.12.2014
Former [2015/09]FI03.09.2014
LT03.09.2014
SE03.09.2014
Former [2015/08]LT03.09.2014
Cited inInternational search[A]EP1672077  (MITSUBISHI CHEM CORP [JP], et al) [A] 1,15,17 * examples 5,6 *;
 [A]WO2007030830  (GENOMATICA INC [US], et al) [A] 10,12 * page 1, line 31 - page 2, line 3 * * example 1 * * claims 1,5,7-9,13,15,27,28 *;
 [DX]WO2007061590  (TATE & LYLE INGREDIENTS [US], et al) [DX] 15-17 * the whole document * * example 1 *;
 [PX]EP1867727  (UNIV DANMARKS TEKNISKE [DK]) [PX] 1,4,7,8,13,15-17 * paragraph [0040] * * paragraphs [0043] - [0052] * * examples 1,2 ** claims 1-12 *;
 [XY]  - DE JONGH W A ET AL, "Enhanced citrate production through gene insertion in Aspergillus niger", METABOLIC ENGINEERING, (20071117), vol. 10, no. 2, ISSN 1096-7176, pages 87 - 96, XP022510142 [X] 1,4,7,8,13,15-17 * the whole document * * page 91, column R, lines 1-8 * * page 92, column R, line 8 - page 93, column L, line 9 * * paragraphs 4.2-5 * * figure 6 * [Y] 2
 [XY]  - DE JONGH W A, "Organic acid production by Aspergillus niger", PHD THESIS, BioCentrum-DTU Technical University of Denmark, (200605), pages I - 109, XP002445685 [X] 1,4,7,8,13,15-17 * the whole document * * chapter 6 (6.2 and 6.3) * * chapter 7 * [Y] 2
 [Y]  - "Fumarate reductase (NADH) (EC 1.3.1.6) (NADH-dependent fumarate reductase) (FAD-dependent oxidoreductase FRDS).", UniProt, (19931001), Database accession no. P32614, URL: EBI, XP002477929 [Y] 2 * the whole document *
 [Y]  - ENOMOTO K ET AL, "Physiological role of soluble fumarate reductase in redox balancing during anaerobiosis in Saccharomyces cerevisiae", FEMS MICROBIOLOGY LETTERS, (20020924), vol. 215, no. 1, ISSN 0378-1097, pages 103 - 108, XP002477926 [Y] 2 * the whole document *

DOI:   http://dx.doi.org/10.1111/j.1574-6968.2002.tb11377.x
 [A]  - LIN H ET AL, "Metabolic engineering of aerobic succinate production systems in Escherichia coli to improve process productivity and achieve the maximum theoretical succinate yield", METABOLIC ENGINEERING, (200503), vol. 7, no. 2, ISSN 1096-7176, pages 116 - 127, XP004801711 [A] 12 * the whole document *

DOI:   http://dx.doi.org/10.1016/j.ymben.2004.10.003
 [A]  - KUBO Y ET AL, "Effect of gene disruption of succinate dehydrogenase on succinate production in a sake yeast strain", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, (2000), vol. 90, no. 6, ISSN 1389-1723, pages 619 - 624, XP003009625 [A] 12 * abstract *

DOI:   http://dx.doi.org/10.1263/jbb.90.619
 [A]  - "Mitochondrial NADH-dependent fumarate reductase (EC 1.3.1.6).", UniProt, (20050412), Database accession no. Q5BU14, URL: EBI, XP002477927 [A] 1-3 * the whole document *
 [A]  - COUSTOU V ET AL, "A mitochondrial NADH-dependent fumarate reductase involved in the production of succinate excreted by procyclic Trypanosoma brucei", JOURNAL OF BIOLOGICAL CHEMISTRY, (200504), vol. 280, no. 17, ISSN 0021-9258, pages 16559 - 16570, XP002477924 [A] 1-3 * the whole document * * figure 1 * * page 16561, right-hand column, paragraph 'Expresion of EGFP-tagged FRDm1 in Trypanosomes' *

DOI:   http://dx.doi.org/10.1074/jbc.M500343200
 [A]  - "NADH-dependent fumarate reductase.", UniProt, (20030301), Database accession no. Q8ITL2, URL: EBI, XP002477928 [A] 1-3 * the whole document *
 [A]  - BESTEIRO S ET AL, "Succinate secreted by Trypanosoma brucei is produced by a novel and unique glycosomal enzyme, NADH-dependent fumarate reductase.", JOURNAL OF BIOLOGICAL CHEMISTRY, (20021011), vol. 277, no. 41, ISSN 0021-9258, pages 38001 - 38012, XP002477925 [A] 1-3 * the whole document * * figure 10 *

DOI:   http://dx.doi.org/10.1074/jbc.M201759200
 [A]  - SONG ET AL, "Production of succinic acid by bacterial fermentation", ENZYME AND MICROBIAL TECHNOLOGY, (20060703), vol. 39, no. 3, ISSN 0141-0229, pages 352 - 361, XP005459365 [A] 15 * the whole document *

DOI:   http://dx.doi.org/10.1016/j.enzmictec.2005.11.043
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DOI:   http://dx.doi.org/10.1002/jctb.1590
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DOI:   http://dx.doi.org/10.1002/YEA.1467
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DOI:   http://dx.doi.org/10.1038/nbt1519
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DOI:   http://dx.doi.org/10.1002/(SICI)1097-0134(20000101)38:1<95::AID-PROT10>3.0.CO;2-A
    - MAY G.S. ET AL, "The Importance of Fungi to Man.", GENOME RESEARCH, (1997), vol. 7, pages 1041 - 1044, XP003031420
    - FLORES C.L. ET AL, "Carbohydrate and energy-yielding metabolism in non-conventional yeasts.", FEMS, (2000), vol. 24, pages 507 - 529, XP003031421
    - WAKAI Y. ET AL, "Formation of succinate fermentation of sake mash and grape must.", STRUCTURE OF SUCCINATE FORMATION, (1960), no. 5, pages 1 - 10, XP003031422
    - KARNIELY S. ET AL, "Single translation-dual destination: mechanisms of dual protein targetting in eukaryotes.", EMBO REPORTS, (2005), vol. 6, no. 6, pages 420 - 425, XP003031423

DOI:   http://dx.doi.org/10.1038/sj.embor.7400394
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