blank Quick help
blank Maintenance news

Scheduled maintenance

Regular maintenance outages:
between 05.00 and 05.15 hrs CET (Monday to Sunday).

Other outages
Availability

2022.02.11

More...
blank News flashes

News Flashes

New version of the European Patent Register – SPC proceedings information in the Unitary Patent Register.

2024-07-24

More...
blank Related links

Release notes
European Patent Register Assistant
European Patent Register Resource Book
European Patent Register – pocket guide
FAQ - European Patent Register
Federated Register (Flyer)
Global Dossier (Flyer)
Register events
Deep link coverage
Federated Register content
The Patent Information Tour
Fair use charter

Extract from the Register of European Patents

EP About this file: EP2138590

EP2138590 - Improved method for the prevention of carryover contamination in nucleic acid amplification technologies [Right-click to bookmark this link]
StatusNo opposition filed within time limit
Status updated on  05.04.2013
Database last updated on 19.10.2024
Most recent event   Tooltip18.07.2014Lapse of the patent in a contracting state
New state(s): HU		published on 20.08.2014  [2014/34]
Applicant(s)For:DE 
Roche Diagnostics GmbH
Sandhofer Strasse 116
68308 Mannheim / DE
For:AT  BE  BG  CH  CY  CZ  DK  EE  ES  FI  FR  GB  GR  HR  HU  IE  IS  IT  LI  LT  LU  LV  MC  MK  MT  NL  NO  PL  PT  RO  SE  SI  SK  TR 
F. Hoffmann-La Roche AG
Grenzacherstrasse 124
4070 Basel / CH
[N/P]
Former [2010/01]For:DE 
Roche Diagnostics GmbH
Sandhofer Strasse 116
68308 Mannheim / DE
For:AT  BE  BG  CH  CY  CZ  DK  EE  ES  FI  FR  GB  GR  HR  HU  IE  IS  IT  LI  LT  LU  LV  MC  MK  MT  NL  NO  PL  PT  RO  SE  SI  SK  TR 
F. Hoffmann-La Roche AG
Grenzacherstrasse 124
4070 Basel / CH
Inventor(s)01 / Bohenzky, Roy
870 E. El Camino Real
Apt. No. 115
Mountain View California 94040 / US
02 / Gupta, Amar
115 Garner Place
Danville CA 94526 / US
03 / Montiel, Janine
37202 Yolo Terrace
Freemont California 94536 / US
04 / Will, Stephen Gordon
1832 Woodhaven Way
Oakland California 94611 / US
 [2012/22]
Former [2010/01]01 / Bohenzky, Roy
870 E. El Camino Real Apt. No. 115
Mountain View California 94040 / US
02 / Gupta, Amar
115 Garner Place
Danville CA 94526 / US
03 / Montiel, Janine
37202 Yolo Terrace
Freemont California 94536 / US
04 / Will, Stephen Gordon
1832 Woodhaven Way
Oakland California 94611 / US
Representative(s)(deleted)
[2012/22]
Application number, filing date09008229.824.06.2009
[2010/01]
Priority number, dateUS20080076111P26.06.2008         Original published format: US 76111 P
[2010/01]
Filing languageEN
Procedural languageEN
PublicationType: A1 Application with search report 
No.:EP2138590
Date:30.12.2009
Language:EN
[2009/53]
Type: B1 Patent specification 
No.:EP2138590
Date:30.05.2012
Language:EN
[2012/22]
Search report(s)(Supplementary) European search report - dispatched on:EP22.10.2009
ClassificationIPC:C12Q1/68, // C12N9/24
[2010/01]
CPC:
C12Q1/6848 (EP,US)
C-Set:
C12Q1/6848, C12Q2521/531, C12Q2525/119, C12Q2527/125 (EP,US)
Designated contracting statesAT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   LV,   MC,   MK,   MT,   NL,   NO,   PL,   PT,   RO,   SE,   SI,   SK,   TR [2010/01]
TitleGerman:Verbessertes Verfahren zur Prävention der Verschleppungskontamination in Nukleinsäureverstärkungstechnologien[2010/01]
English:Improved method for the prevention of carryover contamination in nucleic acid amplification technologies[2010/01]
French:Procédé amélioré pour la prévention d'une contamination par report dans des technologies d'amplification d'acide nucléique[2010/01]
Examination procedure25.06.2010Amendment by applicant (claims and/or description)
30.06.2010Examination requested  [2010/32]
27.08.2010Despatch of a communication from the examining division (Time limit: M06)
22.02.2011Reply to a communication from the examining division
05.04.2011Despatch of a communication from the examining division (Time limit: M04)
21.07.2011Reply to a communication from the examining division
28.12.2011Communication of intention to grant the patent
02.04.2012Fee for grant paid
02.04.2012Fee for publishing/printing paid
Divisional application(s)The date of the Examining Division's first communication in respect of the earliest application for which a communication has been issued is  27.08.2010
Opposition(s)01.03.2013No opposition filed within time limit [2013/19]
Fees paidRenewal fee
07.06.2011Renewal fee patent year 03
Opt-out from the exclusive  Tooltip
competence of the Unified
Patent Court		 See the Register of the Unified Patent Court for opt-out data
Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Lapses during opposition  TooltipHU24.06.2009
AT30.05.2012
BE30.05.2012
CY30.05.2012
CZ30.05.2012
DK30.05.2012
EE30.05.2012
FI30.05.2012
HR30.05.2012
LT30.05.2012
LV30.05.2012
MK30.05.2012
MT30.05.2012
NL30.05.2012
PL30.05.2012
RO30.05.2012
SE30.05.2012
SI30.05.2012
SK30.05.2012
TR30.05.2012
IE24.06.2012
LU24.06.2012
MC30.06.2012
BG30.08.2012
NO30.08.2012
GR31.08.2012
IS30.09.2012
PT01.10.2012
[2014/34]
Former [2014/27]AT30.05.2012
BE30.05.2012
CY30.05.2012
CZ30.05.2012
DK30.05.2012
EE30.05.2012
FI30.05.2012
HR30.05.2012
LT30.05.2012
LV30.05.2012
MK30.05.2012
MT30.05.2012
NL30.05.2012
PL30.05.2012
RO30.05.2012
SE30.05.2012
SI30.05.2012
SK30.05.2012
TR30.05.2012
IE24.06.2012
LU24.06.2012
MC30.06.2012
BG30.08.2012
NO30.08.2012
GR31.08.2012
IS30.09.2012
PT01.10.2012
Former [2014/21]AT30.05.2012
BE30.05.2012
CY30.05.2012
CZ30.05.2012
DK30.05.2012
EE30.05.2012
FI30.05.2012
HR30.05.2012
LT30.05.2012
LV30.05.2012
MK30.05.2012
MT30.05.2012
NL30.05.2012
PL30.05.2012
RO30.05.2012
SE30.05.2012
SI30.05.2012
SK30.05.2012
TR30.05.2012
IE24.06.2012
MC30.06.2012
BG30.08.2012
NO30.08.2012
GR31.08.2012
IS30.09.2012
PT01.10.2012
Former [2013/34]AT30.05.2012
BE30.05.2012
CY30.05.2012
CZ30.05.2012
DK30.05.2012
EE30.05.2012
FI30.05.2012
HR30.05.2012
LT30.05.2012
LV30.05.2012
MK30.05.2012
MT30.05.2012
NL30.05.2012
PL30.05.2012
RO30.05.2012
SE30.05.2012
SI30.05.2012
SK30.05.2012
IE24.06.2012
MC30.06.2012
BG30.08.2012
NO30.08.2012
GR31.08.2012
IS30.09.2012
PT01.10.2012
Former [2013/20]AT30.05.2012
BE30.05.2012
CY30.05.2012
CZ30.05.2012
DK30.05.2012
EE30.05.2012
FI30.05.2012
HR30.05.2012
LT30.05.2012
LV30.05.2012
MK30.05.2012
NL30.05.2012
PL30.05.2012
RO30.05.2012
SE30.05.2012
SI30.05.2012
SK30.05.2012
IE24.06.2012
MC30.06.2012
NO30.08.2012
GR31.08.2012
IS30.09.2012
PT01.10.2012
Former [2013/13]AT30.05.2012
BE30.05.2012
CY30.05.2012
CZ30.05.2012
DK30.05.2012
EE30.05.2012
FI30.05.2012
HR30.05.2012
LT30.05.2012
LV30.05.2012
MK30.05.2012
NL30.05.2012
PL30.05.2012
RO30.05.2012
SE30.05.2012
SI30.05.2012
SK30.05.2012
MC30.06.2012
NO30.08.2012
GR31.08.2012
IS30.09.2012
PT01.10.2012
Former [2013/12]AT30.05.2012
BE30.05.2012
CY30.05.2012
CZ30.05.2012
DK30.05.2012
EE30.05.2012
FI30.05.2012
HR30.05.2012
LT30.05.2012
LV30.05.2012
MK30.05.2012
NL30.05.2012
PL30.05.2012
RO30.05.2012
SE30.05.2012
SI30.05.2012
SK30.05.2012
MC30.06.2012
NO30.08.2012
GR31.08.2012
IS30.09.2012
Former [2013/11]AT30.05.2012
BE30.05.2012
CY30.05.2012
CZ30.05.2012
DK30.05.2012
EE30.05.2012
FI30.05.2012
HR30.05.2012
LT30.05.2012
LV30.05.2012
NL30.05.2012
PL30.05.2012
RO30.05.2012
SE30.05.2012
SI30.05.2012
SK30.05.2012
MC30.06.2012
NO30.08.2012
GR31.08.2012
IS30.09.2012
Former [2013/10]AT30.05.2012
BE30.05.2012
CY30.05.2012
CZ30.05.2012
DK30.05.2012
EE30.05.2012
FI30.05.2012
HR30.05.2012
LT30.05.2012
LV30.05.2012
NL30.05.2012
RO30.05.2012
SE30.05.2012
SI30.05.2012
SK30.05.2012
MC30.06.2012
NO30.08.2012
GR31.08.2012
IS30.09.2012
Former [2013/09]AT30.05.2012
BE30.05.2012
CY30.05.2012
DK30.05.2012
EE30.05.2012
FI30.05.2012
HR30.05.2012
LT30.05.2012
LV30.05.2012
NL30.05.2012
SE30.05.2012
SI30.05.2012
MC30.06.2012
NO30.08.2012
GR31.08.2012
IS30.09.2012
Former [2013/08]AT30.05.2012
BE30.05.2012
CY30.05.2012
DK30.05.2012
FI30.05.2012
HR30.05.2012
LT30.05.2012
LV30.05.2012
NL30.05.2012
SE30.05.2012
SI30.05.2012
MC30.06.2012
NO30.08.2012
GR31.08.2012
IS30.09.2012
Former [2013/07]AT30.05.2012
BE30.05.2012
CY30.05.2012
FI30.05.2012
HR30.05.2012
LT30.05.2012
LV30.05.2012
SE30.05.2012
SI30.05.2012
NO30.08.2012
GR31.08.2012
IS30.09.2012
Former [2013/04]BE30.05.2012
CY30.05.2012
FI30.05.2012
HR30.05.2012
LT30.05.2012
LV30.05.2012
SE30.05.2012
SI30.05.2012
NO30.08.2012
GR31.08.2012
IS30.09.2012
Former [2012/51]CY30.05.2012
FI30.05.2012
HR30.05.2012
LT30.05.2012
LV30.05.2012
SE30.05.2012
SI30.05.2012
NO30.08.2012
GR31.08.2012
IS30.09.2012
Former [2012/50]CY30.05.2012
FI30.05.2012
HR30.05.2012
LT30.05.2012
LV30.05.2012
SE30.05.2012
NO30.08.2012
GR31.08.2012
IS30.09.2012
Former [2012/49]CY30.05.2012
FI30.05.2012
LT30.05.2012
SE30.05.2012
NO30.08.2012
IS30.09.2012
Former [2012/48]FI30.05.2012
LT30.05.2012
SE30.05.2012
NO30.08.2012
Former [2012/47]LT30.05.2012
NO30.08.2012
Documents cited:Search[X]WO9712061  (EPICENTRE TECHNOLOGIES CORP [US]) [X] 8-11,13-15 * figures 1-5; claims 1-25 *;
 [X]EP1038974  (MIRA DIAGNOSTICA GMBH [DE]) [X] 1-4,7-11,13-15 * figures 2-4; claims 1-12 *;
 [X]EP1041159  (MIRA DIAGNOSTIKA GMBH [DE]) [X] 8-11,13-15 * figures 1-4; claims 1-14 *;
 [DXY]US6287823  (HARTLEY JAMES L [US]) [DX] 1-4,7-11,13-15 * column 1, paragraph 3 - paragraph 4 * * column 2, paragraph 2 - paragraph 3 * * column 19, paragraph 2; claims 1-51 * [Y] 5,6,12;
 [DXY]US2002072095  (HARTLEY JAMES L [US], et al) [DX] 1-4,7-11,13-15 * paragraph [0004] - paragraph [0005] * * paragraph [0010] - paragraph [0011] * * paragraph [0156]; claims 1-46 * [Y] 5,6,12;
 [DA]US6413747  (KATO SHINGO [JP], et al) [DA] 1-15 * the whole document *;
 [X]WO02090536  (NOVOZYMES AS [DK], et al) [X] 8-11,13-15 * page 20, line 12 - line 21 * * page 22, line 1 - line 30 * * page 23, line 17 - line 34 * * claims 1-16 *;
 [DY]  - STEULLET V ET AL, "Cleavage of abasic sites in DNA by intercalator-amines.", BIOORGANIC & MEDICINAL CHEMISTRY NOV 1999, (199911), vol. 7, no. 11, ISSN 0968-0896, pages 2531 - 2540, XP002549953 [DY] 5,6,12 * the whole document *

DOI:   http://dx.doi.org/10.1016/S0968-0896(99)00193-5
 [Y]  - MCHUGH P J AND KNOWLAND J, "Novel reagents for chemical cleavage at abasic sites and UV photoproducts in DNA", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, (19950101), vol. 23, no. 10, ISSN 0305-1048, pages 1664 - 1670, XP002086180 [Y] 5,6,12 * the whole document *
 [Y]  - BAILLY V ET AL, "The multiple activities of Escherichia coli endonuclease IV and the extreme lability of 5'-terminal base-free deoxyribose 5-phosphates.", THE BIOCHEMICAL JOURNAL 1 MAY 1989, (19890501), vol. 259, no. 3, ISSN 0264-6021, pages 761 - 768, XP002549954 [Y] 5,6,12 * the whole document *
 [Y]  - WILLIAMS M V ET AL, "A mollicute (mycoplasma) DNA repair enzyme: purification and characterization of uracil-DNA glycosylase.", JOURNAL OF BACTERIOLOGY JUN 1990, (199006), vol. 172, no. 6, ISSN 0021-9193, pages 2979 - 2985, XP002549955 [Y] 5,6,12 * figure 3 *
 [DA]  - HITOMI ET AL, "The intricate structural chemistry of base excision repair machinery: Implications for DNA damage recognition, removal, and repair", DNA REPAIR, ELSEVIER, AMSTERDAM, NL, (20070308), vol. 6, no. 4, ISSN 1568-7864, pages 410 - 428, XP005918191 [DA] 1-15 * the whole document *

DOI:   http://dx.doi.org/10.1016/j.dnarep.2006.10.004
 [DA]  - KLEIBOEKER STEVEN B, "Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR", VIROLOGY JOURNAL, BIOMED CENTRAL, LONDON, GB, (20050411), vol. 2, no. 1, ISSN 1743-422X, page 29, XP021010871 [DA] 1-15 * the whole document *

DOI:   http://dx.doi.org/10.1186/1743-422X-2-29
 [A]  - THORNTON C G ET AL, "Utilizing uracil DNA glycosylase to control carryover contamination in PCR: Characterizationof residual UDG activity following thermal cycling", BIOTECHNIQUES, INFORMA LIFE SCIENCES PUBLISHING, WESTBOROUGH, MA, US, (19930101), vol. 13, no. 2, ISSN 0736-6205, page 180,182,184, XP002113111 [A] 1-15 * the whole document *
 [DA]  - LONGO M C ET AL, "USE OF URACIL DNA GLYCOSYLASE TO CONTROL CARRY-OVER CONTAMINATION IN POLYMERASE CHAIN REACTIONS", GENE, ELSEVIER, AMSTERDAM, NL, (19900101), vol. 93, no. 1, ISSN 0378-1119, pages 125 - 128, XP000371626 [DA] 1-15 * the whole document *

DOI:   http://dx.doi.org/10.1016/0378-1119(90)90145-H
by applicantJPH11113573B
 JPH06277061B
 JPH089997B
 JP2001008680
 US6187575
 US6287823
 US6413747
 US6518026
 US2003077637
 US6713294
    - KROKAN H.E. ET AL., "Uracil in DNA - occurrence, consequences and repair", ONCOGENE, (2002), vol. 21, pages 8935 - 9232
    - BRANSTEITTER R. ET AL., "First AID (Activation-induced cytidine deaminase) is needed to produce high affinity isotype-switched antibodies", J. BIO. CHEM., (2007), vol. 281, pages 16833 - 16836
    - LONGO ET AL., "Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reaction", GENE, (1990), vol. 93, pages 125 - 128
    - SIKORSKY, J.A. ET AL., "DNA damage reduces Taq polymerase fidelity and PCR amplification efficiency", BIOCHEM. BIOPHYS. RES. COMMUN., (2007), vol. 355, pages 431 - 437
    - KOBAYASHI, A. ET AL., "Novel PCR-mediated mutagenesis employing DNA containing a natural abasic site as a template and translesional Taq DNA polymerase", J. BIOTECH., (2005), vol. 116, pages 227 - 232
    - HITOMI ET AL., DNA REPAIR, (2007), vol. 6, pages 410 - 428
    - KLEIBOEKER, "Quantitative detection of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription PCR", VIROLOGY JOURNAL, (2005), vol. 2, page 29
    - STEULLET ET AL., "Cleavage of abasic sites in DNA by intercalator amines", BIOORG. MEDIC. CHEM., (1999), vol. 7, pages 2531 - 2540
    - AHOKAS H.; M. J. ERKKILA, "Interference of PCR amplification by the polyamines, spermine and spermidine", PCR METHODS APPL., (1993), vol. 3, pages 65 - 68
The EPO accepts no responsibility for the accuracy of data originating from other authorities; in particular, it does not guarantee that it is complete, up to date or fit for specific purposes.