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Extract from the Register of European Patents

EP About this file: EP2751268

EP2751268 - METHOD FOR THE ISOLATION OF GENOMIC DNA, RNA, PROTEINS AND METABOLITES FROM A SINGLE BIOLOGICAL SAMPLE [Right-click to bookmark this link]
Former [2014/28]METHOD AND KIT FOR THE ISOLATION OF GENOMIC DNA, RNA, PROTEINS AND METABOLITES FROM A SINGLE BIOLOGICAL SAMPLE
[2015/34]
StatusNo opposition filed within time limit
Status updated on  10.02.2017
Database last updated on 25.09.2024
Most recent event   Tooltip12.10.2018Lapse of the patent in a contracting state
New state(s): AL		published on 14.11.2018  [2018/46]
Applicant(s)For all designated states
Université du Luxembourg
162 A, Avenue de la Faïencerie
1511 Luxembourg / LU
For all designated states
Luxembourg Institute of Science and Technology (LIST)
5, avenue des Hauts-Fourneaux
4362 Esch-sur-Alzette / LU
[2016/08]
Former [2014/28]For all designated states
Université du Luxembourg
162 A, Avenue de la Faïencerie
1511 Luxembourg / LU
For all designated states
Centre de Recherche Public - Gabriel Lippmann
41, rue du Brill
4422 Belvaux / LU
Inventor(s)01 / WILMES, Paul
24 rue du Stade J.F. Kennedy
3502 Dudelange / LU
02 / ROUME, Hugo
154 route d'Esch
1471 Luxembourg / LU
03 / HILLER, Karsten
Birkenweg 6
66663 Besseringen / DE
04 / CORDES, Thekla
30 rue Glesener
1630 Luxembourg / LU
 [2016/14]
Former [2014/28]01 / WILMES, Paul
24 rue du Stade J.F. Kennedy
L-3502 Dudelange / LU
02 / ROUME, Hugo
154 route d'Esch
L-1471 Luxembourg / LU
03 / HILLER, Karsten
Birkenweg 6
66663 Besseringen / DE
04 / CORDES, Thekla
30 rue Glesener
L-1630 Luxembourg / LU
Representative(s)Lecomte & Partners
76-78, rue de Merl
2146 Luxembourg / LU
[N/P]
Former [2014/28]Lecomte & Partners
P.O. Box 1623
1016 Luxembourg / LU
Application number, filing date12745471.802.08.2012
[2016/14]
WO2012EP65178
Priority number, dateLU2011009186402.09.2011         Original published format: LU 91864
[2014/28]
Filing languageEN
Procedural languageEN
PublicationType: A1 Application with search report
No.:WO2013029919
Date:07.03.2013
Language:EN
[2013/10]
Type: A1 Application with search report 
No.:EP2751268
Date:09.07.2014
Language:EN
The application published by WIPO in one of the EPO official languages on 07.03.2013 takes the place of the publication of the European patent application.
[2014/28]
Type: B1 Patent specification 
No.:EP2751268
Date:06.04.2016
Language:EN
[2016/14]
Search report(s)International search report - published on:EP07.03.2013
ClassificationIPC:C12N15/10, C12Q1/68, G01N33/68, C12N1/06
[2015/34]
CPC:
C12N1/066 (EP,US); C12Q1/6806 (US); C12N15/1003 (EP,US);
C12N15/101 (EP,US); G01N33/6803 (US)
Former IPC [2014/28]C12N15/10
Designated contracting statesAL,   AT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   LV,   MC,   MK,   MT,   NL,   NO,   PL,   PT,   RO,   RS,   SE,   SI,   SK,   SM,   TR [2014/28]
Extension statesBANot yet paid
MENot yet paid
TitleGerman:VERFAHREN ZUR ISOLIERUNG VON GENOMISCHER DNA, RNA, PROTEINEN UND METABOLITEN AUS EINER BIOLOGISCHEN PROBE[2015/34]
English:METHOD FOR THE ISOLATION OF GENOMIC DNA, RNA, PROTEINS AND METABOLITES FROM A SINGLE BIOLOGICAL SAMPLE[2015/34]
French:MÉTHODE POUR ISOLER L'ADN, L'ARN, LES PROTÉINES GÉNOMIQUES ET LES MÉTABOLITES D'UN SEUL ÉCHANTILLON BIOLOGIQUE[2015/34]
Former [2014/28]VERFAHREN UND KIT ZUR ISOLIERUNG VON GENOMISCHER DNA, RNA, PROTEINEN UND METABOLITEN AUS EINER BIOLOGISCHEN PROBE
Former [2014/28]METHOD AND KIT FOR THE ISOLATION OF GENOMIC DNA, RNA, PROTEINS AND METABOLITES FROM A SINGLE BIOLOGICAL SAMPLE
Former [2014/28]MÉTHODE ET KIT POUR ISOLER L'ADN, L'ARN, LES PROTÉINES GÉNOMIQUES ET LES MÉTABOLITES D'UN SEUL ÉCHANTILLON BIOLOGIQUE
Entry into regional phase11.03.2014National basic fee paid 
11.03.2014Designation fee(s) paid 
11.03.2014Examination fee paid 
Examination procedure11.03.2014Examination requested  [2014/28]
13.11.2014Amendment by applicant (claims and/or description)
25.08.2015Communication of intention to grant the patent
28.12.2015Fee for grant paid
28.12.2015Fee for publishing/printing paid
28.12.2015Receipt of the translation of the claim(s)
Divisional application(s)The date of the Examining Division's first communication in respect of the earliest application for which a communication has been issued is  25.08.2015
Opposition(s)10.01.2017No opposition filed within time limit [2017/11]
Fees paidRenewal fee
28.08.2014Renewal fee patent year 03
19.08.2015Renewal fee patent year 04
Opt-out from the exclusive  Tooltip
competence of the Unified
Patent Court		 See the Register of the Unified Patent Court for opt-out data
Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Lapses during opposition  TooltipHU02.08.2012
AL06.04.2016
AT06.04.2016
BE06.04.2016
BG06.04.2016
CY06.04.2016
CZ06.04.2016
DK06.04.2016
EE06.04.2016
ES06.04.2016
FI06.04.2016
HR06.04.2016
IT06.04.2016
LT06.04.2016
LV06.04.2016
MC06.04.2016
MK06.04.2016
NL06.04.2016
PL06.04.2016
RO06.04.2016
RS06.04.2016
SE06.04.2016
SI06.04.2016
SK06.04.2016
SM06.04.2016
TR06.04.2016
NO06.07.2016
GR07.07.2016
GB02.08.2016
IE02.08.2016
LU02.08.2016
IS06.08.2016
PT08.08.2016
CH31.08.2016
FR31.08.2016
LI31.08.2016
MT31.08.2016
[2018/46]
Former [2018/45]HU02.08.2012
AT06.04.2016
BE06.04.2016
BG06.04.2016
CY06.04.2016
CZ06.04.2016
DK06.04.2016
EE06.04.2016
ES06.04.2016
FI06.04.2016
HR06.04.2016
IT06.04.2016
LT06.04.2016
LV06.04.2016
MC06.04.2016
MK06.04.2016
NL06.04.2016
PL06.04.2016
RO06.04.2016
RS06.04.2016
SE06.04.2016
SI06.04.2016
SK06.04.2016
SM06.04.2016
TR06.04.2016
NO06.07.2016
GR07.07.2016
GB02.08.2016
IE02.08.2016
LU02.08.2016
IS06.08.2016
PT08.08.2016
CH31.08.2016
FR31.08.2016
LI31.08.2016
MT31.08.2016
Former [2018/38]HU02.08.2012
AT06.04.2016
BE06.04.2016
BG06.04.2016
CY06.04.2016
CZ06.04.2016
DK06.04.2016
EE06.04.2016
ES06.04.2016
FI06.04.2016
HR06.04.2016
IT06.04.2016
LT06.04.2016
LV06.04.2016
MC06.04.2016
MK06.04.2016
NL06.04.2016
PL06.04.2016
RO06.04.2016
RS06.04.2016
SE06.04.2016
SI06.04.2016
SK06.04.2016
SM06.04.2016
NO06.07.2016
GR07.07.2016
GB02.08.2016
IE02.08.2016
LU02.08.2016
IS06.08.2016
PT08.08.2016
CH31.08.2016
FR31.08.2016
LI31.08.2016
MT31.08.2016
Former [2018/31]HU02.08.2012
AT06.04.2016
BE06.04.2016
CY06.04.2016
CZ06.04.2016
DK06.04.2016
EE06.04.2016
ES06.04.2016
FI06.04.2016
HR06.04.2016
IT06.04.2016
LT06.04.2016
LV06.04.2016
MC06.04.2016
MK06.04.2016
NL06.04.2016
PL06.04.2016
RO06.04.2016
RS06.04.2016
SE06.04.2016
SI06.04.2016
SK06.04.2016
SM06.04.2016
NO06.07.2016
GR07.07.2016
GB02.08.2016
IE02.08.2016
LU02.08.2016
IS06.08.2016
PT08.08.2016
CH31.08.2016
FR31.08.2016
LI31.08.2016
MT31.08.2016
Former [2018/28]HU02.08.2012
AT06.04.2016
BE06.04.2016
CY06.04.2016
CZ06.04.2016
DK06.04.2016
EE06.04.2016
ES06.04.2016
FI06.04.2016
HR06.04.2016
IT06.04.2016
LT06.04.2016
LV06.04.2016
MC06.04.2016
MK06.04.2016
NL06.04.2016
PL06.04.2016
RO06.04.2016
RS06.04.2016
SE06.04.2016
SI06.04.2016
SK06.04.2016
SM06.04.2016
NO06.07.2016
GR07.07.2016
GB02.08.2016
IE02.08.2016
LU02.08.2016
IS06.08.2016
PT08.08.2016
CH31.08.2016
FR31.08.2016
LI31.08.2016
Former [2017/37]AT06.04.2016
BE06.04.2016
CZ06.04.2016
DK06.04.2016
EE06.04.2016
ES06.04.2016
FI06.04.2016
HR06.04.2016
IT06.04.2016
LT06.04.2016
LV06.04.2016
MC06.04.2016
NL06.04.2016
PL06.04.2016
RO06.04.2016
RS06.04.2016
SE06.04.2016
SI06.04.2016
SK06.04.2016
SM06.04.2016
NO06.07.2016
GR07.07.2016
GB02.08.2016
IE02.08.2016
LU02.08.2016
IS06.08.2016
PT08.08.2016
CH31.08.2016
FR31.08.2016
LI31.08.2016
Former [2017/23]AT06.04.2016
BE06.04.2016
CZ06.04.2016
DK06.04.2016
EE06.04.2016
ES06.04.2016
FI06.04.2016
HR06.04.2016
IT06.04.2016
LT06.04.2016
LV06.04.2016
MC06.04.2016
NL06.04.2016
PL06.04.2016
RO06.04.2016
RS06.04.2016
SE06.04.2016
SI06.04.2016
SK06.04.2016
SM06.04.2016
NO06.07.2016
GR07.07.2016
IS06.08.2016
PT08.08.2016
CH31.08.2016
LI31.08.2016
Former [2017/11]AT06.04.2016
BE06.04.2016
CZ06.04.2016
DK06.04.2016
EE06.04.2016
ES06.04.2016
FI06.04.2016
HR06.04.2016
IT06.04.2016
LT06.04.2016
LV06.04.2016
NL06.04.2016
PL06.04.2016
RO06.04.2016
RS06.04.2016
SE06.04.2016
SK06.04.2016
SM06.04.2016
NO06.07.2016
GR07.07.2016
IS06.08.2016
PT08.08.2016
Former [2017/10]AT06.04.2016
BE06.04.2016
CZ06.04.2016
DK06.04.2016
EE06.04.2016
ES06.04.2016
FI06.04.2016
HR06.04.2016
IT06.04.2016
LT06.04.2016
LV06.04.2016
NL06.04.2016
PL06.04.2016
RO06.04.2016
RS06.04.2016
SE06.04.2016
SK06.04.2016
NO06.07.2016
GR07.07.2016
IS06.08.2016
PT08.08.2016
Former [2017/09]AT06.04.2016
BE06.04.2016
CZ06.04.2016
DK06.04.2016
EE06.04.2016
ES06.04.2016
FI06.04.2016
HR06.04.2016
IT06.04.2016
LT06.04.2016
LV06.04.2016
NL06.04.2016
PL06.04.2016
RO06.04.2016
RS06.04.2016
SE06.04.2016
NO06.07.2016
GR07.07.2016
IS06.08.2016
PT08.08.2016
Former [2017/07]AT06.04.2016
BE06.04.2016
DK06.04.2016
ES06.04.2016
FI06.04.2016
HR06.04.2016
IT06.04.2016
LT06.04.2016
LV06.04.2016
NL06.04.2016
PL06.04.2016
RS06.04.2016
SE06.04.2016
NO06.07.2016
GR07.07.2016
IS06.08.2016
PT08.08.2016
Former [2017/03]AT06.04.2016
BE06.04.2016
ES06.04.2016
FI06.04.2016
HR06.04.2016
IT06.04.2016
LT06.04.2016
LV06.04.2016
NL06.04.2016
PL06.04.2016
RS06.04.2016
SE06.04.2016
NO06.07.2016
GR07.07.2016
IS06.08.2016
PT08.08.2016
Former [2016/52]AT06.04.2016
ES06.04.2016
FI06.04.2016
HR06.04.2016
LT06.04.2016
LV06.04.2016
NL06.04.2016
PL06.04.2016
RS06.04.2016
SE06.04.2016
NO06.07.2016
GR07.07.2016
IS06.08.2016
PT08.08.2016
Former [2016/51]AT06.04.2016
ES06.04.2016
FI06.04.2016
HR06.04.2016
LT06.04.2016
LV06.04.2016
NL06.04.2016
PL06.04.2016
RS06.04.2016
NO06.07.2016
GR07.07.2016
IS06.08.2016
PT08.08.2016
Former [2016/50]AT06.04.2016
ES06.04.2016
FI06.04.2016
HR06.04.2016
LT06.04.2016
LV06.04.2016
NL06.04.2016
PL06.04.2016
RS06.04.2016
NO06.07.2016
IS06.08.2016
Former [2016/49]FI06.04.2016
LT06.04.2016
NL06.04.2016
PL06.04.2016
NO06.07.2016
IS06.08.2016
Former [2016/48]LT06.04.2016
NL06.04.2016
NO06.07.2016
IS06.08.2016
Former [2016/46]LT06.04.2016
NL06.04.2016
NO06.07.2016
Former [2016/44]NL06.04.2016
Cited inInternational search[A]US2004224344  (HAN XIAOLIANG [US], et al) [A] 1-15 * the whole document *;
 [YDA]  - WOLFRAM WECKWERTH ET AL, "Process for the integrated extraction, identification and quantification of metabolites, proteins and RNA to reveal their co-regulation in biochemical networks", PROTEOMICS, (20040101), vol. 4, no. 1, doi:10.1002/pmic.200200500, ISSN 1615-9853, pages 78 - 83, XP055013545 [YD] 15 * the whole document * [A] 1-14

DOI:   http://dx.doi.org/10.1002/pmic.200200500
 [YA]  - Norgen Biotek Corp., "All-in-One Purification Kit Product #24200", (2008), pages 1 - 26, URL: http://www.norgenbiotek.com/product_resources/allinone_purification_kit_total_rna_microrna_total_proteins_and_genomic_dna_allinone_purification_kit__protocol_24200_110.pdf, (20111201), XP002664869 [Y] 15 * the whole document * [A] 1-14
 [YDA]  - GROSS, "Tissue fractionation by hydrostatic pressure cycling technology: the unified sample preparation technique for systems biology studies", JOURNAL OF BIOMOLECULAR TECHNIQUES, (20080101), vol. 19, no. 3, ISSN 1524-0215, page 189, XP055013562 [YD] 15 * the whole document * [A] 1-14
 [A]  - SOROTH CHEY ET AL, "Improved method for simultaneous isolation of proteins and nucleic acids", ANALYTICAL BIOCHEMISTRY, (20110401), vol. 411, no. 1, doi:10.1016/j.ab.2010.11.020, ISSN 0003-2697, pages 164 - 166, XP055013548 [A] 1-15 * the whole document *

DOI:   http://dx.doi.org/10.1016/j.ab.2010.11.020
 [A]  - SHIBKO S ET AL, "A method for sequential quantitative separation and determination of protein, RNA, DNA, lipid, and glycogen from a single rat liver homogenate or from a subcellular fraction", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS INC, NEW YORK, vol. 19, no. 3, doi:10.1016/0003-2697(67)90242-4, ISSN 0003-2697, (19670601), pages 514 - 528, (19670601), XP024827657 [A] 1-15 * the whole document *

DOI:   http://dx.doi.org/10.1016/0003-2697(67)90242-4
by applicantUS2005106604
 US7488579
 WO2009070558
    - HUMMON AB; LIM SR; DIFILIPPANTONIO MJ; RIED T., "Isolation and solubilization of proteins after TRlzol extraction of RNA and DNA from patient material following prolonged storage", BIOTECHNIQUES, (2007), vol. 4, pages 467 - 70,472
    - CHEY S; CLAUS C; LIEBERT UG, "Improved method for simultaneous isolation of proteins and nucleic acids", ANALYTICAL BIOCHEMISTRY, (2011), vol. 1, pages 164 - 166
    - XIONG J; YANG Q; KANG J; SUN Y; ZHANG T; MARGARET G ET AL., "Simultaneous isolation of DNA, RNA, and protein from Medicago truncatula L.", ELECTROPHORESIS, (2011), vol. 2, pages 321 - 330
    - WECKWERTH W; WENZEL K; FIEHN O., "Process for the integrated extraction, identification and quantification of metabolites, proteins and RNA to reveal their co-regulation in biochemical networks", PROTEOMICS, (2004), vol. 1, pages 78 - 83
    - MORSE SM; SHAW G; LARNER SF., "Concurrent mRNA and protein extraction from the same experimental sample using a commercially available column-based RNA preparation kit", BIOTECHNIQUES, (2006), vol. 1, page 54,56,58
    - TOLOSA JM; SCHJENKEN JE; CIVITI TD; CLIFTON VL; SMITH R, "Column-based method to simultaneously extract DNA, RNA, and proteins from the same sample", BIOTECHNIQUES, (2007), vol. 6, pages 799 - 804
    - GROSS V; CARLSON G; KWAN AT; SMEJKAL G; FREEMAN E; IVANOV AR ET AL., "Tissue fractionation by hydrostatic pressure cycling technology: the unified sample preparation technique for systems biology studies", J BIOMOL TECH, (2008), vol. 3, pages 189 - 199
    - CHOMCZYNSKI, P, "A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples", BIOTECHNIQUES, (1993), vol. 15, pages 532 - 36,236
    - WILMES P.; BOND PL., "The application of two-dimensional polyacrylamide gel electrophoresis and downstream analyses to a mixed community of prokaryotic microorganisms", ENVIRONMENTAL MICROBIOLOGY, (2004), vol. 9, pages 911 - 920
The EPO accepts no responsibility for the accuracy of data originating from other authorities; in particular, it does not guarantee that it is complete, up to date or fit for specific purposes.