| EP2734538 - METHOD OF REVERSIBLY STAINING A TARGET CELL [Right-click to bookmark this link] | Status | Patent maintained as amended Status updated on 31.05.2024 Database last updated on 21.03.2026 | |
| Former | The patent has been granted Status updated on 30.03.2018 | ||
| Former | Grant of patent is intended Status updated on 07.03.2018 | ||
| Former | Examination is in progress Status updated on 02.11.2016 | Most recent event Tooltip | 31.05.2024 | Patent maintained (B2 publication) | published on 03.07.2024 [2024/27] | Applicant(s) | For all designated states IBA Lifesciences GmbH Rudolf-Wissell-Str. 28 37079 Göttingen / DE | [2021/45] |
| Former [2014/22] | For all designated states IBA GmbH Rudolf-Wissell-Strasse 28 37079 Göttingen / DE | Inventor(s) | 01 /
SCHMIDT, Thomas Tabakswinkel 8 37139 Adelebsen / DE | 02 /
STEMBERGER, Christian Franz-von-Defregger-Str. 17 83607 Holzkirchen / DE | 03 /
BUSCH, Dirk, H. Havelstr. 6a 81677 Muenchen / DE | 04 /
GERMEROTH, Lothar Am Menzelberg 16a 37077 Goettingen / DE | [2014/22] | Representative(s) | Schiweck Weinzierl Koch Patentanwälte Partnerschaft mbB Ganghoferstraße 68 B 80339 München / DE | [2024/27] |
| Former [2018/18] | Schiweck, Weinzierl & Koch Patentanwälte Partnerschaft mbB Landsberger Straße 98 80339 München / DE | ||
| Former [2014/22] | Schiweck, Weinzierl & Koch European Patent Attorneys Landsberger Straße 98 80339 München / DE | Application number, filing date | 12747985.5 | 17.07.2012 | [2018/18] | WO2012EP63969 | Priority number, date | US201161508943P | 18.07.2011 Original published format: US 201161508943 P | [2014/22] | Filing language | EN | Procedural language | EN | Publication | Type: | A2 Application without search report | No.: | WO2013011011 | Date: | 24.01.2013 | Language: | EN | [2013/04] | Type: | A2 Application without search report | No.: | EP2734538 | Date: | 28.05.2014 | Language: | EN | The application published by WIPO in one of the EPO official languages on 24.01.2013 takes the place of the publication of the European patent application. | [2014/22] | Type: | B1 Patent specification | No.: | EP2734538 | Date: | 02.05.2018 | Language: | EN | [2018/18] | Type: | B2 New European patent specification | No.: | EP2734538 | Date: | 03.07.2024 | Language: | EN | [2024/27] | Search report(s) | International search report - published on: | EP | 02.05.2013 | Classification | IPC: | C07K14/36, G01N33/50 | [2014/22] | CPC: |
G01N33/56972 (EP,CN,US);
G01N1/30 (EP,CN,US);
A61K35/14 (EP,US);
A61K35/17 (EP,CN,US);
A61P1/00 (EP);
A61P3/10 (EP);
A61P31/04 (EP);
A61P31/12 (EP);
A61P35/00 (EP);
A61P35/02 (EP);
A61P37/06 (EP);
A61P37/08 (EP);
G01N33/557 (EP,CN,US);
G01N33/566 (US);
C07K2319/00 (EP,CN,US);
C07K2319/22 (EP,CN,US);
C07K2319/74 (EP,CN,US);
G01N2333/705 (US);
G01N2458/00 (EP,US)
(-)
| Designated contracting states | AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LI, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR [2014/22] | Title | German: | VERFAHREN ZUR UMKEHRBAREN FÄRBUNG EINER ZIELZELLE | [2014/22] | English: | METHOD OF REVERSIBLY STAINING A TARGET CELL | [2014/22] | French: | PROCÉDÉ POUR LA COLORATION RÉVERSIBLE D'UNE CELLULE CIBLE | [2014/22] | Entry into regional phase | 10.02.2014 | National basic fee paid | 10.02.2014 | Designation fee(s) paid | 10.02.2014 | Examination fee paid | Examination procedure | 10.02.2014 | Amendment by applicant (claims and/or description) | 10.02.2014 | Examination requested [2014/22] | 02.12.2014 | Despatch of a communication from the examining division (Time limit: M06) | 09.06.2015 | Reply to a communication from the examining division | 25.06.2015 | Despatch of a communication from the examining division (Time limit: M06) | 09.11.2015 | Reply to a communication from the examining division | 15.12.2015 | Despatch of a communication from the examining division (Time limit: M04) | 22.04.2016 | Reply to a communication from the examining division | 21.06.2016 | Despatch of a communication from the examining division (Time limit: M06) | 14.12.2016 | Observations by third parties | 22.12.2016 | Reply to a communication from the examining division | 30.01.2017 | Despatch of a communication from the examining division (Time limit: M06) | 03.08.2017 | Reply to a communication from the examining division | 11.10.2017 | Despatch of a communication from the examining division (Time limit: M02) | 13.11.2017 | Reply to a communication from the examining division | 08.03.2018 | Communication of intention to grant the patent | 21.03.2018 | Fee for grant paid | 21.03.2018 | Fee for publishing/printing paid | 21.03.2018 | Receipt of the translation of the claim(s) | Divisional application(s) | EP18168713.8 / EP3418295 | The date of the Examining Division's first communication in respect of the earliest application for which a communication has been issued is 02.12.2014 | Opposition(s) | Opponent(s) | 01
01.02.2019
06.02.2019
ADMISSIBLE Miltenyi Biotech B.V. & Co. KG Friedrich-Ebert Straße 68 51429 Bergisch Gladbach / DE Opponent's representative Kisters, Michael Marcus Miltenyi Biotec B.V. & Co. KG Friedrich-Ebert Strasse 68 51429 Bergisch Gladbach / DE | [2020/42] |
| Former [2019/11] | |||
| Opponent(s) | 01
01.02.2019
06.02.2019
ADMISSIBLE Miltenyi Biotec GmbH Friedrich-Ebert-Str 68 51429 Bergisch-Gladbach / DE | 13.02.2019 | Invitation to proprietor to file observations on the notice of opposition | 16.09.2020 | Cancellation of oral proceeding that was planned for 16.11.2020 | 16.11.2020 | Date of oral proceedings | 13.01.2022 | Legal effect of interlocutory decision in opposition | 13.01.2022 | Date of oral proceedings | 25.01.2022 | Despatch of interlocutory decision in opposition | 25.01.2022 | Despatch of minutes of oral proceedings | 01.03.2024 | Despatch of communication that the patent will be maintained as amended | Appeal following opposition | 04.04.2022 | Appeal received No. T0879/22 | 04.04.2022 | Payment of appeal fee | 03.06.2022 | Statement of grounds filed | 13.02.2024 | Result of appeal procedure: appeal of the proprietor withdrawn | Fees paid | Renewal fee | 23.07.2014 | Renewal fee patent year 03 | 23.07.2015 | Renewal fee patent year 04 | 22.07.2016 | Renewal fee patent year 05 | 24.07.2017 | Renewal fee patent year 06 |
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| Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court. | Lapses during opposition Tooltip | HU | 17.07.2012 | AL | 02.05.2018 | CY | 02.05.2018 | DK | 02.05.2018 | EE | 02.05.2018 | FI | 02.05.2018 | HR | 02.05.2018 | LT | 02.05.2018 | LV | 02.05.2018 | MC | 02.05.2018 | MK | 02.05.2018 | RO | 02.05.2018 | RS | 02.05.2018 | SM | 02.05.2018 | LU | 17.07.2018 | MT | 17.07.2018 | BG | 02.08.2018 | IS | 02.09.2018 | [2020/33] |
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| LT | 02.05.2018 | Cited in | International search | [XY] US5985658 (COLINAS ROBERT JAMES et al.) [Y] 12-14,16-21,23-25,33-36 | [X] WO02054065 (IBA GMBH et al.) [X] 1-8,12,26-32 * the whole document * | [X] TIM SCHROEDER: "Nach Zellen angeln", FASZINATION FORSCHUNG, 30 June 2010 (2010-06-30), pages 28 - 37, XP002687294, Retrieved from the Internet | [XY] ANITA SCHMITT ET AL: "Adoptive transfer and selective reconstitution of streptamer-selected cytomegalovirus-specific CD8+ T?cells leads to virus clearance in patients after allogeneic peripheral blood stem cell transplantation", TRANSFUSION, vol. 51, no. 3, 6 December 2010 (2010-12-06), pages 591 - 599, XP055043927, ISSN: 0041-1132, DOI: 10.1111/j.1537-2995.2010.02940.x DOI: http://dx.doi.org/10.1111/j.1537-2995.2010.02940.x | [X] KNABEL MICHAEL ET AL: "Reversible MHC multimer staining for functional isolation of T-cell populations and effective adoptive transfer", NATURE MEDICINE, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 8, no. 6, 1 June 2002 (2002-06-01), pages 631 - 637, XP002460640, ISSN: 1078-8956, DOI: 10.1038/NM0602-631 [X] 1-11,15-18,22-24,26-36 * the whole document * DOI: http://dx.doi.org/10.1038/nm0602-631 | [XY] MATSUI K ET AL: "KINETICS OF T-CELL RECEPTOR BINDING TO PEPTIDE/I-EK COMPLEXES CORRELATION OF THE DISSOCIATION RATE WITH T-CELL RESPONSIVENESS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, US, vol. 91, 1 December 1994 (1994-12-01), pages 12862 - 12866, XP002008363, ISSN: 0027-8424, DOI: 10.1073/PNAS.91.26.12862 [X] 1-11,15,22,26-32 * the whole document * [Y] 12-14,16-21,23-25,33-36 DOI: http://dx.doi.org/10.1073/pnas.91.26.12862 | [XY] ODENDAHL M K ET AL: "GMP COMPLIANT ISOLATION OF UNTOUCHED CMV-SPECIFIC DONOR DERIVED CD3+CD8+T LYMPHOCYTES USING STREPTAMER-TECHNOLOGY", VOX SANGUINIS, S. KARGER AG, BASEL, CH, vol. 99, no. Suppl.1, 1 June 2010 (2010-06-01), pages 490 - 491, XP009164629, ISSN: 0042-9007, [retrieved on 20100625] [X] 1-11,15,22,26-32 * the whole document * * figure 3b *[Y] 12-14,16-21,23-25,33-36 | [Y] ROSAELY CASALEGNO-GARDU +-O ET AL: "Multimer technologies for detection and adoptive transfer of antigen-specific T cells", CANCER IMMUNOLOGY, IMMUNOTHERAPY, SPRINGER, BERLIN, DE, vol. 59, no. 2, 22 October 2009 (2009-10-22), pages 195 - 202, XP019757721, ISSN: 1432-0851 [Y] 1-36 * abstract * | [XY] NEUDORFER JULIA ET AL: "Reversible HLA multimers (Streptamers) for the isolation of human cytotoxic T lymphocytes functionally active against tumor- and virus-derived antigens", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 320, no. 1-2, 30 March 2007 (2007-03-30), pages 119 - 131, XP002518434, ISSN: 0022-1759, DOI: 10.1016/J.JIM.2007.01.001 [X] 1-11,15,22,26-32 * the whole document *[Y] 12-14,16-21,23-25,33-36 DOI: http://dx.doi.org/10.1016/J.JIM.2007.01.001 | [XY] SCHMIDT THOMAS G M ET AL: "The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins", NATURE PROTOCOLS, NATURE PUBLISHING GROUP, GB, vol. 2, no. 6, 1 January 2007 (2007-01-01), pages 1528 - 1535, XP001536704, ISSN: 1750-2799, DOI: 10.1038/NPROT.2007.209 [X] 1-11,15,22,26-32 * the whole document * * page 197; figure 2a *[Y] 12-14,16-21,23-25,33-36 DOI: http://dx.doi.org/10.1038/nprot.2007.209 | [Y] R CR GIS BOUQUI CR ET AL: "A fast and efficient HLA multimer-based sorting procedure that induces little apoptosis to isolate clinical grade human tumor specific T lymphocytes", CANCER IMMUNOLOGY, IMMUNOTHERAPY, SPRINGER, BERLIN, DE, vol. 58, no. 4, 27 August 2008 (2008-08-27), pages 553 - 566, XP019706296, ISSN: 1432-0851 [Y] 1-36 * the whole document * | [X] CHRISTIAN STEMBERGER ET AL: "Novel Serial Positive Enrichment Technology Enables Clinical Multiparameter Cell Sorting", PLOS ONE, vol. 7, no. 4, 24 April 2012 (2012-04-24), pages e35798, XP055043969, DOI: 10.1371/journal.pone.0035798 [X] 1-8,17,22,26-32 * the whole document * DOI: http://dx.doi.org/10.1371/journal.pone.0035798 | Examination | M. NAUERTH ET AL: "TCR-Ligand koff Rate Correlates with the Protective Capacity of Antigen-Specific CD8+ T Cells for Adoptive Transfer", SCIENCE TRANSLATIONAL MEDICINE, vol. 5, no. 192, 3 July 2013 (2013-07-03), pages 192ra87 - 192ra87, XP055211659, ISSN: 1946-6234, DOI: 10.1126/scitranslmed.3005958 [P] DOI: http://dx.doi.org/10.1126/scitranslmed.3005958 | ANONYMOUS: "Dissociation constant", Retrieved from the Internet | by applicant | US7776562 | WO02054065 | US7482000 | WO2010017103 | US6103493 | US5506121 | WO02077018 | US5985658 | US4851341 | KOLB, H.J. ET AL.: "Donor leukocyte transfusions for treatment of recurrent chronic myelogenous leukemia in marrow transplant patients", BLOOD, vol. 76, no. 12, 1990, pages 2462 - 2465, XP002475033 [Y] 1-36 * the whole document * | RIDDELL, S.R. ET AL.: "Restoration of viral immunity in immunodeficient humans by the adoptive transfer of T cell clones", SCIENCE, vol. 257, no. 5067, 1992, pages 238 - 241, XP001179181, DOI: doi:10.1126/science.1352912 DOI: http://dx.doi.org/10.1126/science.1352912 | ROONEY, C.M. ET AL.: "Use of gene-modified virus-specific T lymphocytes to control Epstein-Barr-virus-related lymphoproliferation", LANCET, vol. 345, no. 8941, 1995, pages 9 - 13, XP000608280, DOI: doi:10.1016/S0140-6736(95)91150-2 [X] 1-8,17,22,26-32 * the whole document * DOI: http://dx.doi.org/10.1016/S0140-6736(95)91150-2 | RILEY, J.L.; JUNE, C.H.; BLAZAR, B.R.: "Human T regulatory cell therapy: take a billion or so and call me in the morning", IMMUNITY, vol. 30, no. 5, 2009, pages 656 - 665, XP055159519, DOI: doi:10.1016/j.immuni.2009.04.006 [I] 12-14,16-21,23-25,33-36 DOI: http://dx.doi.org/10.1016/j.immuni.2009.04.006 | RANDOLPH, D.A.; FATHMAN, C.G.: "Cd4+Cd25+ regulatory T cells and their therapeutic potential", ANNU REV MED, vol. 57, 2006, pages 381 - 402, XP055029165, DOI: doi:10.1146/annurev.med.57.121304.131337 [T] DOI: http://dx.doi.org/10.1146/annurev.med.57.121304.131337 | MIYARA, M. ET AL.: "Functional delineation and differentiation dynamics of human CD4+ T cells expressing the FoxP3 transcription factor", IMMUNITY, vol. 30, no. 6, 2009, pages 899 - 911 | HOFFMANN, P. ET AL.: "Only the CD45RA+ subpopulation of CD4+CD25high T cells gives rise to homogeneous regulatory T-cell lines upon in vitro expansion", BLOOD, vol. 108, no. 13, 2006, pages 4260 - 4267, XP002555048, DOI: doi:10.1182/blood-2006-06-027409 DOI: http://dx.doi.org/10.1182/blood-2006-06-027409 | JONSSON, U. ET AL., BIOTECHNIQUES, vol. 11, 1991, pages 620 - 627 | BES, C. ET AL., JBIOL CHEM, vol. 278, 2003, pages 14265 - 14273 | SCHLAPSCHY, M. ET AL.: "Functional humanization of an anti-CD30 Fab fragment for the immunotherapy of Hodgkin's lymphoma using an in vitro evolution approach", PROTEIN ENG DES SEL, vol. 17, 2004, pages 847 - 860, XP055428135, DOI: doi:10.1093/protein/gzh098 DOI: http://dx.doi.org/10.1093/protein/gzh098 | LEE ET AL., MOL. BIOL., vol. 340, 2004, pages 1073 - 1093 | KWONG ET AL., J. MOL. BIOL., vol. 384, no. 5, 31 December 2008 (2008-12-31), pages 1143 - 1156 | NOGUCHI, A.; TAKAHASHI, T.; YAMAGUCHI, T.; KITAMURA, K.; TAKAKURA, Y.; HASHIDA, M.; SEZAKI, H.: "Preparation and properties of the immunoconjugate composed of anti-human colon cancer monoclonal antibody and mitomycin C dextran conjugate", BIOCONJUGATE CHEMISTRY, vol. 3, 1992, pages 132 - 137, XP000262170, DOI: doi:10.1021/bc00014a007 DOI: http://dx.doi.org/10.1021/bc00014a007 | ILL. ET AL.: "Design and construction of a hybrid immunoglobulin domain with properties of both heavy and light chain variable regions", PROTEIN ENG, vol. 10, 1997, pages 949 - 57, XP002110047, DOI: doi:10.1093/protein/10.8.949 DOI: http://dx.doi.org/10.1093/protein/10.8.949 | MARTIN ET AL.: "The affinity-selection of a minibody polypeptide inhibitor of human interleukin-6", EMBO J, vol. 13, 1994, pages 5303 - 9, XP002076260 | TRAUNECKER ET AL.: "Bispecific single chain molecules (Janusins) target cytotoxic lymphocytes on HIV infected cells", EMBO J, vol. 10, 1991, pages 3655 - 3659, XP000232579 | TRAUNECKER ET AL.: "Janusin: new molecular design for bispecific reagents", INT J CANCER, vol. 7, 1992, pages 51 - 52, XP009171574 | SILVERMAN, LU Q; BAKKER A; TO W; DUGUAY A; ALBA BM; SMITH R; RIVAS A; LI P; LE H; WHITEHORN E, NAT BIOTECH., vol. 23, no. 12, 20 November 2005 (2005-11-20), pages 1556 - 61 | SILVERMAN J; LU Q; BAKKER A; TO W; DUGUAY A; ALBA BM; SMITH R; RIVAS A; LI P; LE H, NAT. BIOTECHNOLOGY, vol. 23, no. 12, 20 November 2005 (2005-11-20), pages 1556 - 61 | LATA ET AL., J. AM. CHEM. SOC., vol. 127, 2005, pages 10205 - 10215 | HUANG ET AL., BIOCONJUGATE CHEM., vol. 17, 2006, pages 1592 - 1600 | SCHMITT ET AL., TRANSFUSION, vol. 51, 2011, pages 591 - 599 | BES, C. ET AL., J BIOL CHEM, vol. 278, 2003, pages 14265 - 14273 | PLCKTHUN, A.; SKERRA, A.: "Expression of functional antibody Fv and Fab fragments in Escherichia coli", METHODS ENZYMOL, vol. 178, 1989, pages 497 - 515, XP002024991, DOI: doi:10.1016/0076-6879(89)78036-8 DOI: http://dx.doi.org/10.1016/0076-6879(89)78036-8 | SCHMIDT, T.G.; SKERRA, A.: "The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins", NAT PROTOC, vol. 2, 2007, pages 1528 - 1535, XP001536704, DOI: doi:10.1038/nprot.2007.209 DOI: http://dx.doi.org/10.1038/nprot.2007.209 | other | WO02054065 | "Dissociation constant", 16 August 2707 (2707-08-16), XP055288111, Retrieved from the Internet |