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Extract from the Register of European Patents

EP About this file: EP2854982

EP2854982 - CHROMATOGRAPHIC PURIFICATION OF POLYNUCLEOTIDES WITH NEGATIVELY CHARGED PARTICLES [Right-click to bookmark this link]
Former [2015/15]Chromatographic Purification Of Polynucleotides With Negatively Charged Particles
[2016/22]
StatusNo opposition filed within time limit
Status updated on  07.09.2018
Database last updated on 18.11.2024
FormerThe patent has been granted
Status updated on  29.09.2017
FormerGrant of patent is intended
Status updated on  11.05.2017
Most recent event   Tooltip17.07.2020Lapse of the patent in a contracting state
New state(s): AL		published on 19.08.2020  [2020/34]
Applicant(s)For all designated states
Agency for Science, Technology and Research
1 Fusionopolis Way 20-10 Connexis
Singapore 138632 / SG
[2015/15]
Inventor(s)01 / GAGNON, Peter
20 Biopolis Way
06-01 Centros
Singapore 138668 / SG
 [2015/15]
Representative(s)Zimmermann, Tankred Klaus, et al
Schoppe, Zimmermann, Stöckeler
Zinkler, Schenk & Partner mbB
Patentanwälte
Radlkoferstrasse 2
81373 München / DE
[2015/15]
Application number, filing date13797414.330.05.2013
[2017/44]
WO2013SG00217
Priority number, dateUS201261653694P31.05.2012         Original published format: US 201261653694 P
US201361773666P06.03.2013         Original published format: US 201361773666 P
[2015/15]
Filing languageEN
Procedural languageEN
PublicationType: A1 Application with search report
No.:WO2013180654
Date:05.12.2013
Language:EN
[2013/49]
Type: A1 Application with search report 
No.:EP2854982
Date:08.04.2015
Language:EN
The application published by WIPO in one of the EPO official languages on 05.12.2013 takes the place of the publication of the European patent application.
[2015/15]
Type: B1 Patent specification 
No.:EP2854982
Date:01.11.2017
Language:EN
[2017/44]
Search report(s)International search report - published on:AU05.12.2013
(Supplementary) European search report - dispatched on:EP27.11.2015
ClassificationIPC:B01D15/14, C12N15/10, B01D15/36
[2015/15]
CPC:
C12N15/1006 (EP,KR,US); C12N15/101 (KR,US); B01D15/362 (EP,KR,US);
C07H21/04 (EP,KR,US); C12N15/1017 (EP,KR,US)
Designated contracting statesAL,   AT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   LV,   MC,   MK,   MT,   NL,   NO,   PL,   PT,   RO,   RS,   SE,   SI,   SK,   SM,   TR [2015/15]
Extension statesBANot yet paid
MENot yet paid
TitleGerman:CHROMATOGRAFISCHE REINIGUNG VON POLYNUKLEOTIDEN MIT NEGATIV GELADENEN PARTIKELN[2015/15]
English:CHROMATOGRAPHIC PURIFICATION OF POLYNUCLEOTIDES WITH NEGATIVELY CHARGED PARTICLES[2016/22]
French:PURIFICATION CHROMATOGRAPHIQUE DE POLYNUCLÉOTIDES COMPORTANT DES PARTICULES CHARGÉES NÉGATIVEMENT[2015/15]
Former [2015/15]Chromatographic Purification Of Polynucleotides With Negatively Charged Particles
Entry into regional phase16.12.2014National basic fee paid 
16.12.2014Search fee paid 
16.12.2014Designation fee(s) paid 
16.12.2014Examination fee paid 
Examination procedure16.12.2014Examination requested  [2015/15]
25.04.2016Amendment by applicant (claims and/or description)
12.05.2017Communication of intention to grant the patent
19.09.2017Fee for grant paid
19.09.2017Fee for publishing/printing paid
19.09.2017Receipt of the translation of the claim(s)
Divisional application(s)The date of the Examining Division's first communication in respect of the earliest application for which a communication has been issued is  12.05.2017
Opposition(s)02.08.2018No opposition filed within time limit [2018/41]
Fees paidRenewal fee
30.04.2015Renewal fee patent year 03
14.03.2016Renewal fee patent year 04
21.04.2017Renewal fee patent year 05
Opt-out from the exclusive  Tooltip
competence of the Unified
Patent Court		 See the Register of the Unified Patent Court for opt-out data
Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Lapses during opposition  TooltipHU30.05.2013
AL01.11.2017
AT01.11.2017
CY01.11.2017
CZ01.11.2017
DK01.11.2017
EE01.11.2017
ES01.11.2017
FI01.11.2017
HR01.11.2017
IT01.11.2017
LT01.11.2017
LV01.11.2017
MC01.11.2017
MK01.11.2017
NL01.11.2017
PL01.11.2017
PT01.11.2017
RO01.11.2017
RS01.11.2017
SI01.11.2017
SK01.11.2017
SM01.11.2017
TR01.11.2017
BG01.02.2018
NO01.02.2018
GR02.02.2018
IS01.03.2018
IE30.05.2018
LU30.05.2018
MT30.05.2018
BE31.05.2018
[2020/34]
Former [2020/31]HU30.05.2013
AT01.11.2017
CY01.11.2017
CZ01.11.2017
DK01.11.2017
EE01.11.2017
ES01.11.2017
FI01.11.2017
HR01.11.2017
IT01.11.2017
LT01.11.2017
LV01.11.2017
MC01.11.2017
MK01.11.2017
NL01.11.2017
PL01.11.2017
PT01.11.2017
RO01.11.2017
RS01.11.2017
SI01.11.2017
SK01.11.2017
SM01.11.2017
TR01.11.2017
BG01.02.2018
NO01.02.2018
GR02.02.2018
IS01.03.2018
IE30.05.2018
LU30.05.2018
MT30.05.2018
BE31.05.2018
Former [2020/30]HU30.05.2013
AT01.11.2017
CY01.11.2017
CZ01.11.2017
DK01.11.2017
EE01.11.2017
ES01.11.2017
FI01.11.2017
HR01.11.2017
IT01.11.2017
LT01.11.2017
LV01.11.2017
MC01.11.2017
NL01.11.2017
PL01.11.2017
PT01.11.2017
RO01.11.2017
RS01.11.2017
SI01.11.2017
SK01.11.2017
SM01.11.2017
TR01.11.2017
BG01.02.2018
NO01.02.2018
GR02.02.2018
IS01.03.2018
IE30.05.2018
LU30.05.2018
MT30.05.2018
BE31.05.2018
Former [2020/28]AT01.11.2017
CY01.11.2017
CZ01.11.2017
DK01.11.2017
EE01.11.2017
ES01.11.2017
FI01.11.2017
HR01.11.2017
IT01.11.2017
LT01.11.2017
LV01.11.2017
MC01.11.2017
NL01.11.2017
PL01.11.2017
PT01.11.2017
RO01.11.2017
RS01.11.2017
SI01.11.2017
SK01.11.2017
SM01.11.2017
TR01.11.2017
BG01.02.2018
NO01.02.2018
GR02.02.2018
IS01.03.2018
IE30.05.2018
LU30.05.2018
MT30.05.2018
BE31.05.2018
Former [2020/16]AT01.11.2017
CY01.11.2017
CZ01.11.2017
DK01.11.2017
EE01.11.2017
ES01.11.2017
FI01.11.2017
HR01.11.2017
IT01.11.2017
LT01.11.2017
LV01.11.2017
MC01.11.2017
NL01.11.2017
PL01.11.2017
RO01.11.2017
RS01.11.2017
SI01.11.2017
SK01.11.2017
SM01.11.2017
TR01.11.2017
BG01.02.2018
NO01.02.2018
GR02.02.2018
IS01.03.2018
IE30.05.2018
LU30.05.2018
MT30.05.2018
BE31.05.2018
Former [2020/08]AT01.11.2017
CY01.11.2017
CZ01.11.2017
DK01.11.2017
EE01.11.2017
ES01.11.2017
FI01.11.2017
HR01.11.2017
IT01.11.2017
LT01.11.2017
LV01.11.2017
MC01.11.2017
NL01.11.2017
PL01.11.2017
RO01.11.2017
RS01.11.2017
SI01.11.2017
SK01.11.2017
SM01.11.2017
BG01.02.2018
NO01.02.2018
GR02.02.2018
IS01.03.2018
IE30.05.2018
LU30.05.2018
MT30.05.2018
BE31.05.2018
Former [2019/24]AT01.11.2017
CY01.11.2017
CZ01.11.2017
DK01.11.2017
EE01.11.2017
ES01.11.2017
FI01.11.2017
HR01.11.2017
IT01.11.2017
LT01.11.2017
LV01.11.2017
MC01.11.2017
NL01.11.2017
PL01.11.2017
RO01.11.2017
RS01.11.2017
SI01.11.2017
SK01.11.2017
SM01.11.2017
BG01.02.2018
NO01.02.2018
GR02.02.2018
IS01.03.2018
IE30.05.2018
LU30.05.2018
BE31.05.2018
Former [2019/19]AT01.11.2017
CY01.11.2017
CZ01.11.2017
DK01.11.2017
EE01.11.2017
ES01.11.2017
FI01.11.2017
HR01.11.2017
IT01.11.2017
LT01.11.2017
LV01.11.2017
MC01.11.2017
NL01.11.2017
PL01.11.2017
RO01.11.2017
RS01.11.2017
SI01.11.2017
SK01.11.2017
SM01.11.2017
BG01.02.2018
NO01.02.2018
GR02.02.2018
IS01.03.2018
IE30.05.2018
LU30.05.2018
Former [2019/17]AT01.11.2017
CY01.11.2017
CZ01.11.2017
DK01.11.2017
EE01.11.2017
ES01.11.2017
FI01.11.2017
HR01.11.2017
IT01.11.2017
LT01.11.2017
LV01.11.2017
MC01.11.2017
NL01.11.2017
PL01.11.2017
RO01.11.2017
RS01.11.2017
SI01.11.2017
SK01.11.2017
SM01.11.2017
BG01.02.2018
NO01.02.2018
GR02.02.2018
IS01.03.2018
LU30.05.2018
Former [2019/06]AT01.11.2017
CY01.11.2017
CZ01.11.2017
DK01.11.2017
EE01.11.2017
ES01.11.2017
FI01.11.2017
HR01.11.2017
IT01.11.2017
LT01.11.2017
LV01.11.2017
MC01.11.2017
NL01.11.2017
PL01.11.2017
RO01.11.2017
RS01.11.2017
SI01.11.2017
SK01.11.2017
SM01.11.2017
BG01.02.2018
NO01.02.2018
GR02.02.2018
IS01.03.2018
Former [2018/52]AT01.11.2017
CY01.11.2017
CZ01.11.2017
DK01.11.2017
EE01.11.2017
ES01.11.2017
FI01.11.2017
HR01.11.2017
IT01.11.2017
LT01.11.2017
LV01.11.2017
NL01.11.2017
PL01.11.2017
RO01.11.2017
RS01.11.2017
SI01.11.2017
SK01.11.2017
SM01.11.2017
BG01.02.2018
NO01.02.2018
GR02.02.2018
IS01.03.2018
Former [2018/40]AT01.11.2017
CY01.11.2017
CZ01.11.2017
DK01.11.2017
EE01.11.2017
ES01.11.2017
FI01.11.2017
HR01.11.2017
IT01.11.2017
LT01.11.2017
LV01.11.2017
NL01.11.2017
PL01.11.2017
RO01.11.2017
RS01.11.2017
SK01.11.2017
SM01.11.2017
BG01.02.2018
NO01.02.2018
GR02.02.2018
IS01.03.2018
Former [2018/39]AT01.11.2017
CY01.11.2017
CZ01.11.2017
DK01.11.2017
EE01.11.2017
ES01.11.2017
FI01.11.2017
HR01.11.2017
IT01.11.2017
LT01.11.2017
LV01.11.2017
NL01.11.2017
PL01.11.2017
RS01.11.2017
SK01.11.2017
SM01.11.2017
BG01.02.2018
NO01.02.2018
GR02.02.2018
IS01.03.2018
Former [2018/37]AT01.11.2017
CY01.11.2017
CZ01.11.2017
DK01.11.2017
EE01.11.2017
ES01.11.2017
FI01.11.2017
HR01.11.2017
LT01.11.2017
LV01.11.2017
NL01.11.2017
PL01.11.2017
RS01.11.2017
SK01.11.2017
SM01.11.2017
BG01.02.2018
NO01.02.2018
GR02.02.2018
IS01.03.2018
Former [2018/35]AT01.11.2017
CY01.11.2017
CZ01.11.2017
DK01.11.2017
EE01.11.2017
ES01.11.2017
FI01.11.2017
HR01.11.2017
LT01.11.2017
LV01.11.2017
NL01.11.2017
RS01.11.2017
SK01.11.2017
BG01.02.2018
NO01.02.2018
GR02.02.2018
IS01.03.2018
Former [2018/24]AT01.11.2017
ES01.11.2017
FI01.11.2017
HR01.11.2017
LT01.11.2017
LV01.11.2017
NL01.11.2017
RS01.11.2017
BG01.02.2018
NO01.02.2018
GR02.02.2018
IS01.03.2018
Former [2018/22]ES01.11.2017
FI01.11.2017
LT01.11.2017
NL01.11.2017
NO01.02.2018
Documents cited:Search[X]  - J. URTHALER ET AL, "Industrial Scale cGMP Purification of Pharmaceutical Grade Plasmid-DNA", CHEMICAL ENGINEERING & TECHNOLOGY, (20051101), vol. 28, no. 11, doi:10.1002/ceat.200500126, ISSN 0930-7516, pages 1408 - 1420, XP055135523 [X] 15 * the whole document *

DOI:   http://dx.doi.org/10.1002/ceat.200500126
 [A]  - GUSTAVSSON P-E ET AL, "Purification of plasmid DNA with a new type of anion-exchange beads having a non-charged surface", JOURNAL OF CHROMATOGRAPHY, ELSEVIER SCIENCE PUBLISHERS B.V, NL, (20040604), vol. 1038, no. 1-2, doi:10.1016/J.CHROMA.2004.03.050, ISSN 0021-9673, pages 131 - 140, XP004508891 [A] 1-15 * the whole document *

DOI:   http://dx.doi.org/10.1016/j.chroma.2004.03.050
 [A]  - KEPKA ET AL, "Integrated process for purification of plasmid DNA using aqueous two-phase systems combined with membrane filtration and lid bead chromatography", JOURNAL OF CHROMATOGRAPHY, ELSEVIER SCIENCE PUBLISHERS B.V, NL, (20041119), vol. 1057, no. 1-2, doi:10.1016/J.CHROMA.2004.09.060, ISSN 0021-9673, pages 115 - 124, XP005003879 [A] 1-15 * the whole document *

DOI:   http://dx.doi.org/10.1016/j.chroma.2004.09.060
 [A]  - G. N. M. FERREIRA, "Chromatographic Approaches in the Purification of Plasmid DNA for Therapy and Vaccination", CHEMICAL ENGINEERING AND TECHNOLOGY, DE, (20051101), vol. 28, no. 11, doi:10.1002/ceat.200500158, ISSN 0930-7516, pages 1285 - 1294, XP055228392 [A] 1-14 * the whole document *

DOI:   http://dx.doi.org/10.1002/ceat.200500158
 [AD]  - HUNTER A K ET AL, "Protein adsorption on novel acrylamido-based polymeric ion-exchangers - I. Morphology and equilibrium adsorption", JOURNAL OF CHROMATOGRAPHY, ELSEVIER SCIENCE PUBLISHERS B.V, NL, (20001103), vol. 897, no. 1-2, doi:10.1016/S0021-9673(00)00864-5, ISSN 0021-9673, pages 65 - 80, XP004218944 [AD] 1-15 * the whole document *

DOI:   http://dx.doi.org/10.1016/S0021-9673(00)00864-5
 [AD]  - HUNTER A K ET AL, "Protein adsorption on novel acrylamido-based polymeric ion exchangers - II. Adsorption rates and column behavior", JOURNAL OF CHROMATOGRAPHY, ELSEVIER SCIENCE PUBLISHERS B.V, NL, (20001103), vol. 897, no. 1-2, doi:10.1016/S0021-9673(00)00865-7, ISSN 0021-9673, pages 81 - 97, XP004218945 [AD] 1-15 * the whole document *

DOI:   http://dx.doi.org/10.1016/S0021-9673(00)00865-7
 [AD]  - HUNTER A K ET AL, "Protein adsorption on novel acrylamido-based polymeric ion-exchangers - III. Salt concentration effects and elution behavior", JOURNAL OF CHROMATOGRAPHY, ELSEVIER SCIENCE PUBLISHERS B.V, NL, (20010928), vol. 930, no. 1-2, doi:10.1016/S0021-9673(01)01162-1, ISSN 0021-9673, pages 79 - 93, XP004306376 [AD] 1-15 * the whole document *

DOI:   http://dx.doi.org/10.1016/S0021-9673(01)01162-1
 [AD]  - HUNTER A K ET AL, "Protein adsorption on novel acrylamido-based polymeric ion-exchangers - IV. Effects of protein size on adsorption capacity and rate", JOURNAL OF CHROMATOGRAPHY, ELSEVIER SCIENCE PUBLISHERS B.V, NL, (20020920), vol. 971, no. 1-2, doi:10.1016/S0021-9673(02)01027-0, ISSN 0021-9673, pages 105 - 116, XP004380032 [AD] 1-15 * the whole document *

DOI:   http://dx.doi.org/10.1016/S0021-9673(02)01027-0
International search[A]WO2010027696  (GE HEALTHCARE BIO SCIENCES [US], et al);
 [T]WO2012149200  (BIO RAD LABORATORIES [US], et al);
 [X]  - SCHMIDT B ET AL., "Investigation of particle-based and monolithic columns for cation exchange protein displacement chromatography using poly(diallyl-dimethylammonium chloride) as displacer.", J CHROMATOGR A., (2003), vol. 1018, no. 2, pages 155 - 67, XP004463120

DOI:   http://dx.doi.org/10.1016/S0021-9673(03)01326-8
 [A]  - GAGNON, P., "The emerging generation of chromatography tools for virus purification.", BIOPROCESS INTERNATIONAL. 2008, (200810), vol. 6, pages 24 - 30, XP055178342
 [T]  - NIAN R ET AL., "Void exclusion of antibodies by grafted-ligand porous particle anion exchangers.", J CHROMATOGR A., (2013), vol. 1282, pages 127 - 132, XP055098385

DOI:   http://dx.doi.org/10.1016/j.chroma.2013.01.065
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