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Extract from the Register of European Patents

EP About this file: EP3072962

EP3072962 - A METHOD FOR PRODUCING A PHYTASE VARIANT WITH IMPROVED THERMAL STABILITY, AND A PHYTASE VARIANT AND THE USE THEREOF [Right-click to bookmark this link]
StatusNo opposition filed within time limit
Status updated on  01.11.2019
Database last updated on 28.09.2024
FormerThe patent has been granted
Status updated on  23.11.2018
FormerGrant of patent is intended
Status updated on  03.07.2018
FormerExamination is in progress
Status updated on  19.01.2018
Most recent event   Tooltip01.07.2022Lapse of the patent in a contracting state
New state(s): MK		published on 03.08.2022  [2022/31]
Applicant(s)For all designated states
Feed Research Institute Chinese Academy of Agricultural Sciences
No. 12, Zhongguancun South Street
Haidian District
Beijing 100081 / CN
[2016/39]
Inventor(s)01 / YAO, Bin
No.12 Zhongguancun Nandajie
Haidian
Beijing 100081 / CN
02 / HUANG, Huoqing
No.12 Zhongguancun Nandajie
Haidian
Beijing 100081 / CN
03 / LUO, Huiying
No.12 Zhongguancun Nandajie
Haidian
Beijing 100081 / CN
04 / SHAO, Chao
No.12 Zhongguancun Nandajie
Haidian
Beijing 100081 / CN
05 / BAI, Yingguo
No.12 Zhongguancun Nandajie
Haidian
Beijing 100081 / CN
06 / WANG, Yaru
No.12 Zhongguancun Nandajie
Haidian
Beijing 100081 / CN
07 / YANG, Peilong
No.12 Zhongguancun Nandajie
Haidian
Beijing 100081 / CN
08 / SHI, Pengjun
No.12 Zhongguancun Nandajie
Haidian
Beijing 100081 / CN
09 / MENG, Kun
No.12 Zhongguancun Nandajie
Haidian
Beijing100081 / CN
10 / ZHAO, Heng
No.12 Zhongguancun Nandajie
Haidian
Beijing 100081 / CN
11 / MA, Rui
No.12 Zhongguancun Nandajie
Haidian
Beijing 100081 / CN
 [2016/39]
Representative(s)Teall, Charlotte
Forresters IP LLP
Skygarden
Erika-Mann-Strasse 11
80636 München / DE
[2018/52]
Former [2016/39]Teall, Charlotte
Forresters
Skygarden
Erika-Mann-Strasse 11
80636 München / DE
Application number, filing date13897309.412.11.2013
[2016/39]
WO2013CN86943
Filing languageZH
Procedural languageEN
PublicationType: A1 Application with search report
No.:WO2015070372
Date:21.05.2015
Language:ZH
[2015/20]
Type: A1 Application with search report 
No.:EP3072962
Date:28.09.2016
Language:EN
[2016/39]
Type: B1 Patent specification 
No.:EP3072962
Date:26.12.2018
Language:EN
[2018/52]
Search report(s)International search report - published on:CN21.05.2015
(Supplementary) European search report - dispatched on:EP17.05.2017
ClassificationIPC:C12N15/52
[2018/25]
CPC:
C12N9/16 (EP,US); A23K20/189 (EP,US); C12N1/14 (US);
C12N1/20 (US); C12N15/52 (EP,US); C12N15/63 (US);
C12Y301/03 (EP,US) (-)
Former IPC [2016/39]C12N9/16, C12N15/55, C12N15/63, C12N1/14, C12N1/21, A23K20/168
Designated contracting statesAL,   AT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   LV,   MC,   MK,   MT,   NL,   NO,   PL,   PT,   RO,   RS,   SE,   SI,   SK,   SM,   TR [2016/39]
Extension statesBANot yet paid
MENot yet paid
TitleGerman:VERFAHREN ZUR HERSTELLUNG EINER PHYTASE-VARIANTE MIT VERBESSERTER THERMISCHER STABILITÄT, PHYTASE-VARIANTE UND VERWENDUNG DAVON[2016/39]
English:A METHOD FOR PRODUCING A PHYTASE VARIANT WITH IMPROVED THERMAL STABILITY, AND A PHYTASE VARIANT AND THE USE THEREOF[2016/39]
French:PROCÉDÉ DE PRODUCTION D'UN VARIANT DE PHYTASE PRÉSENTANT UNE STABILITÉ THERMIQUE AMÉLIORÉE ET VARIANT DE PHYTASE ET SON UTILISATION[2016/39]
Entry into regional phase21.07.2016Translation filed 
21.07.2016National basic fee paid 
21.07.2016Search fee paid 
21.07.2016Designation fee(s) paid 
21.07.2016Examination fee paid 
Examination proceduredeletedDeletion: Application deemed to be withdrawn, date of legal effect  [2016/39]
19.07.2016Despatch of communication that the application is deemed to be withdrawn, reason: filing fee / search fee not paid in time  [2016/39]
21.07.2016Examination requested  [2016/39]
30.11.2017Amendment by applicant (claims and/or description)
17.01.2018Despatch of a communication from the examining division (Time limit: M04)
10.05.2018Reply to a communication from the examining division
04.07.2018Communication of intention to grant the patent
01.11.2018Fee for grant paid
01.11.2018Fee for publishing/printing paid
01.11.2018Receipt of the translation of the claim(s)
Divisional application(s)The date of the Examining Division's first communication in respect of the earliest application for which a communication has been issued is  17.01.2018
Opposition(s)27.09.2019No opposition filed within time limit [2019/49]
Request for further processing for:The application is deemed to be withdrawn due to failure to file the translation
25.07.2016Request for further processing filed
25.07.2016Full payment received (date of receipt of payment)
Request granted
11.08.2016Decision despatched
The application is deemed to be withdrawn due to non-payment of the search fee
21.07.2016Request for further processing filed
21.07.2016Full payment received (date of receipt of payment)
Request granted
11.08.2016Decision despatched
The application is deemed to be withdrawn due to non-payment of the filing fee
21.07.2016Request for further processing filed
21.07.2016Full payment received (date of receipt of payment)
Request granted
11.08.2016Decision despatched
The application is deemed to be withdrawn due to non-payment of the examination fee
21.07.2016Request for further processing filed
21.07.2016Full payment received (date of receipt of payment)
Request granted
11.08.2016Decision despatched
21.07.2016Request for further processing filed
21.07.2016Full payment received (date of receipt of payment)
Request granted
11.08.2016Decision despatched
The application is deemed to be withdrawn due to non-payment of designation fees
TR, SM, SK, SI, SE, RS, RO, PT, PL, NO, NL, MT, MK, MC, LV, LU, LT, IT, IS, IE, HU, HR, GR, GB, FR, FI, ES, EE, DK, DE, CZ, CY, CH, BG, BE, AT, AL
21.07.2016Request for further processing filed
21.07.2016Full payment received (date of receipt of payment)
Request granted
11.08.2016Decision despatched
Fees paidRenewal fee
21.07.2016Renewal fee patent year 03
27.10.2016Renewal fee patent year 04
20.10.2017Renewal fee patent year 05
16.11.2018Renewal fee patent year 06
Penalty fee
Additional fee for renewal fee
13.06.201603   M06   Fee paid on   21.07.2016
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Lapses during opposition  TooltipHU12.11.2013
AL26.12.2018
AT26.12.2018
CY26.12.2018
CZ26.12.2018
EE26.12.2018
ES26.12.2018
FI26.12.2018
HR26.12.2018
IT26.12.2018
LT26.12.2018
LV26.12.2018
MC26.12.2018
MK26.12.2018
MT26.12.2018
PL26.12.2018
RO26.12.2018
RS26.12.2018
SE26.12.2018
SI26.12.2018
SK26.12.2018
SM26.12.2018
TR26.12.2018
BG26.03.2019
NO26.03.2019
GR27.03.2019
IS26.04.2019
PT26.04.2019
[2022/31]
Former [2021/34]HU12.11.2013
AL26.12.2018
AT26.12.2018
CY26.12.2018
CZ26.12.2018
EE26.12.2018
ES26.12.2018
FI26.12.2018
HR26.12.2018
IT26.12.2018
LT26.12.2018
LV26.12.2018
MC26.12.2018
MT26.12.2018
PL26.12.2018
RO26.12.2018
RS26.12.2018
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SI26.12.2018
SK26.12.2018
SM26.12.2018
TR26.12.2018
BG26.03.2019
NO26.03.2019
GR27.03.2019
IS26.04.2019
PT26.04.2019
Former [2021/32]HU12.11.2013
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CY26.12.2018
CZ26.12.2018
EE26.12.2018
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FI26.12.2018
HR26.12.2018
IT26.12.2018
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MC26.12.2018
PL26.12.2018
RO26.12.2018
RS26.12.2018
SE26.12.2018
SI26.12.2018
SK26.12.2018
SM26.12.2018
TR26.12.2018
BG26.03.2019
NO26.03.2019
GR27.03.2019
IS26.04.2019
PT26.04.2019
Former [2021/26]AL26.12.2018
AT26.12.2018
CY26.12.2018
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EE26.12.2018
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IT26.12.2018
LT26.12.2018
LV26.12.2018
MC26.12.2018
PL26.12.2018
RO26.12.2018
RS26.12.2018
SE26.12.2018
SI26.12.2018
SK26.12.2018
SM26.12.2018
TR26.12.2018
BG26.03.2019
NO26.03.2019
GR27.03.2019
IS26.04.2019
PT26.04.2019
Former [2020/36]AL26.12.2018
AT26.12.2018
CZ26.12.2018
EE26.12.2018
ES26.12.2018
FI26.12.2018
HR26.12.2018
IT26.12.2018
LT26.12.2018
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MC26.12.2018
PL26.12.2018
RO26.12.2018
RS26.12.2018
SE26.12.2018
SI26.12.2018
SK26.12.2018
SM26.12.2018
TR26.12.2018
BG26.03.2019
NO26.03.2019
GR27.03.2019
IS26.04.2019
PT26.04.2019
Former [2020/17]AL26.12.2018
AT26.12.2018
CZ26.12.2018
EE26.12.2018
ES26.12.2018
FI26.12.2018
HR26.12.2018
IT26.12.2018
LT26.12.2018
LV26.12.2018
PL26.12.2018
RO26.12.2018
RS26.12.2018
SE26.12.2018
SI26.12.2018
SK26.12.2018
SM26.12.2018
TR26.12.2018
BG26.03.2019
NO26.03.2019
GR27.03.2019
IS26.04.2019
PT26.04.2019
Former [2020/13]AL26.12.2018
AT26.12.2018
CZ26.12.2018
EE26.12.2018
ES26.12.2018
FI26.12.2018
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IT26.12.2018
LT26.12.2018
LV26.12.2018
PL26.12.2018
RO26.12.2018
RS26.12.2018
SE26.12.2018
SI26.12.2018
SK26.12.2018
SM26.12.2018
BG26.03.2019
NO26.03.2019
GR27.03.2019
IS26.04.2019
PT26.04.2019
Former [2019/46]AL26.12.2018
AT26.12.2018
CZ26.12.2018
EE26.12.2018
ES26.12.2018
FI26.12.2018
HR26.12.2018
IT26.12.2018
LT26.12.2018
LV26.12.2018
PL26.12.2018
RO26.12.2018
RS26.12.2018
SE26.12.2018
SK26.12.2018
SM26.12.2018
BG26.03.2019
NO26.03.2019
GR27.03.2019
IS26.04.2019
PT26.04.2019
Former [2019/39]AL26.12.2018
CZ26.12.2018
EE26.12.2018
ES26.12.2018
FI26.12.2018
HR26.12.2018
IT26.12.2018
LT26.12.2018
LV26.12.2018
PL26.12.2018
RO26.12.2018
RS26.12.2018
SE26.12.2018
SK26.12.2018
SM26.12.2018
BG26.03.2019
NO26.03.2019
GR27.03.2019
IS26.04.2019
PT26.04.2019
Former [2019/37]AL26.12.2018
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EE26.12.2018
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FI26.12.2018
HR26.12.2018
IT26.12.2018
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LV26.12.2018
PL26.12.2018
RO26.12.2018
RS26.12.2018
SE26.12.2018
SM26.12.2018
BG26.03.2019
NO26.03.2019
GR27.03.2019
PT26.04.2019
Former [2019/35]AL26.12.2018
CZ26.12.2018
ES26.12.2018
FI26.12.2018
HR26.12.2018
IT26.12.2018
LT26.12.2018
LV26.12.2018
PL26.12.2018
RS26.12.2018
SE26.12.2018
BG26.03.2019
NO26.03.2019
GR27.03.2019
PT26.04.2019
Former [2019/34]AL26.12.2018
FI26.12.2018
HR26.12.2018
IT26.12.2018
LT26.12.2018
LV26.12.2018
RS26.12.2018
SE26.12.2018
BG26.03.2019
NO26.03.2019
GR27.03.2019
PT26.04.2019
Former [2019/33]AL26.12.2018
FI26.12.2018
HR26.12.2018
LT26.12.2018
LV26.12.2018
RS26.12.2018
SE26.12.2018
BG26.03.2019
NO26.03.2019
GR27.03.2019
PT26.04.2019
Former [2019/26]AL26.12.2018
FI26.12.2018
HR26.12.2018
LT26.12.2018
LV26.12.2018
RS26.12.2018
SE26.12.2018
BG26.03.2019
NO26.03.2019
GR27.03.2019
Former [2019/25]FI26.12.2018
HR26.12.2018
LT26.12.2018
LV26.12.2018
RS26.12.2018
SE26.12.2018
BG26.03.2019
NO26.03.2019
GR27.03.2019
Former [2019/24]FI26.12.2018
HR26.12.2018
LT26.12.2018
LV26.12.2018
SE26.12.2018
BG26.03.2019
NO26.03.2019
GR27.03.2019
Former [2019/23]FI26.12.2018
HR26.12.2018
LT26.12.2018
LV26.12.2018
BG26.03.2019
NO26.03.2019
Former [2019/22]FI26.12.2018
LT26.12.2018
BG26.03.2019
NO26.03.2019
Former [2019/21]FI26.12.2018
LT26.12.2018
NO26.03.2019
Former [2019/20]LT26.12.2018
NO26.03.2019
Documents cited:Search[A]WO2008055625  (AB ENZYMES GMBH [DE], et al) [A] 1-12 * Claims *;
 [X]  - A. R. PAGANO ET AL, "Distribution of supplemental AppA2 phytase activity in digesta of various gastrointestinal segments of young pigs", JOURNAL OF ANIMAL SCIENCE, (20070101), vol. 85, no. 6, doi:10.2527/jas.2006-111, pages 1444 - 1452, XP055365848 [X] 1,3-12 * the whole document *

DOI:   http://dx.doi.org/10.2527/jas.2006-111
 [X]  - "Escherichia coli Lei appA2 phytase protein.", Geneseq, (20100722), Database accession no. AYB66043, URL: EBI, XP002769391 [X] 1,3-12 * sequence . *
 [X]  - MOON-SOO KIM ET AL, "Enhancing thermostability of Escherichia coli phytase AppA2 by error-prone PCR", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, (20080314), vol. 79, no. 1, ISSN 1432-0614, pages 69 - 75, XP019623565 [X] 1,3-12 * the whole document *
 [T]  - PRIYABRATA PANIGRAHI ET AL, "Engineering Proteins for Thermostability with iRDP Web Server", PLOS ONE, (20151005), vol. 10, no. 10, doi:10.1371/journal.pone.0139486, page e0139486, XP055365182

DOI:   http://dx.doi.org/10.1371/journal.pone.0139486
    [ ] - "Sequence 18 from Patent WO2008055625.", EPO Proteins, (20081218), Database accession no. GM037630, URL: EBI, XP002769389 [ ] * sequence . *
 [A]  - WANMING ZHANG ET AL, "Cumulative improvements of thermostability and pH-activity profile of Aspergillus niger PhyA phytase by site-directed mutagenesis", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, (20071030), vol. 77, no. 5, ISSN 1432-0614, pages 1033 - 1040, XP019560768 [A] 1-12 * First paragraph of page 1039 *
 [A]  - VOHRA A ET AL, "PHYTASES: MICROBIAL SOURCES, PRODUCTION, PURIFICATION, AND POTENTIAL BIOTECHNOLOGICAL APPLICATIONS", CRC CRITICAL REVIEWS IN BIOTECHNOLOGY, CRC PRESS, BOCA RATON, FL, US, (20030101), vol. 23, no. 1, ISSN 0738-8551, pages 29 - 60, XP008060235 [A] 1-12 * Item E. *
International search[A]CN1423694  (CORNELL RES FOUNDATION INC [US]);
 [A]CN1706941  (ANHUI GUYUAN BIOENGINEERING CO [CN]);
 [A]CN102002487  (GUANGDONG VTR BIO TECH CO LTD);
 [A]CN102209785  (FUJIAN FUDA BIOTECH CO LTD);
 [A]CN102392002  (QINGDAO BLUE BIOLOG GROUP CO LTD, et al);
 [A]  - "Accession No. 1DKLA, Chain A, Crystal Structure of Escherichia Coli Phytase At Ph 4.5 (No Ligand Bound", GENBANK, (20121010), pages 1 - 2, XP055293419
by applicantWO2008036916
 WO2009073399
    - DASSA ET AL., J.BACT., (1990), pages 5497 - 5500
    - HUANG ET AL., APPL MICROBIOL BIOTECHNOL, (2008), vol. 80, no. 3, pages 417 - 26
The EPO accepts no responsibility for the accuracy of data originating from other authorities; in particular, it does not guarantee that it is complete, up to date or fit for specific purposes.