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Extract from the Register of European Patents

EP About this file: EP2971282

EP2971282 - MICROVESICLE HISTONE H2AX AS A BIOMARKER FOR GENOTOXIC STRESS [Right-click to bookmark this link]
StatusNo opposition filed within time limit
Status updated on  26.07.2019
Database last updated on 14.09.2024
FormerThe patent has been granted
Status updated on  17.08.2018
FormerGrant of patent is intended
Status updated on  11.04.2018
FormerExamination is in progress
Status updated on  25.08.2017
Most recent event   Tooltip01.07.2022Lapse of the patent in a contracting state
New state(s): MK		published on 03.08.2022  [2022/31]
Applicant(s)For all designated states
Pioma Inc.
1827 South Bayshore Lane
Miami, FL 33133 / US
[2016/03]
Inventor(s)01 / EZRIN, Alan, M.
1827 South Bayshore Lane
Miami, FL 33133 / US
 [2016/03]
Representative(s)Zacco Sweden AB
P.O. Box 5581
Löjtnantsgatan 21
114 85 Stockholm / SE
[N/P]
Former [2016/03]Zacco Sweden AB
P.O. Box 5581
114 85 Stockholm / SE
Application number, filing date14767965.814.03.2014
[2016/03]
WO2014US28079
Priority number, dateUS201361785552P14.03.2013         Original published format: US 201361785552 P
[2016/03]
Filing languageEN
Procedural languageEN
PublicationType: A1 Application with search report
No.:WO2014152873
Date:25.09.2014
Language:EN
[2014/39]
Type: A1 Application with search report 
No.:EP2971282
Date:20.01.2016
Language:EN
The application published by WIPO in one of the EPO official languages on 25.09.2014 takes the place of the publication of the European patent application.
[2016/03]
Type: B1 Patent specification 
No.:EP2971282
Date:19.09.2018
Language:EN
[2018/38]
Search report(s)International search report - published on:US25.09.2014
(Supplementary) European search report - dispatched on:EP16.12.2016
ClassificationIPC:C12Q1/6883, C12Q1/6886
[2018/17]
CPC:
G01N33/6875 (EP,US); G01N33/57496 (US); C12Q1/6883 (EP,US);
C12Q1/6886 (EP,US); C12Q2600/142 (EP,US); C12Q2600/158 (EP,US);
G01N2333/4703 (US); G01N2440/14 (US); G01N2800/7004 (EP,US) (-)
Former IPC [2016/03]C40B30/04, C12Q1/68, G01N33/566
Designated contracting statesAL,   AT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   LV,   MC,   MK,   MT,   NL,   NO,   PL,   PT,   RO,   RS,   SE,   SI,   SK,   SM,   TR [2016/03]
Extension statesBANot yet paid
MENot yet paid
TitleGerman:MIKROVESIKELHISTON H2AX ALS BIOMARKER FÜR GENOTOXISCHEN STRESS[2016/03]
English:MICROVESICLE HISTONE H2AX AS A BIOMARKER FOR GENOTOXIC STRESS[2016/03]
French:HISTONE H2AX MICROVÉSICULAIRE EN TANT QUE BIOMARQUEUR POUR LE STRESS GÉNOTOXIQUE[2016/03]
Entry into regional phase15.09.2015National basic fee paid 
15.09.2015Search fee paid 
15.09.2015Designation fee(s) paid 
15.09.2015Examination fee paid 
Examination procedure15.09.2015Examination requested  [2016/03]
06.07.2017Amendment by applicant (claims and/or description)
28.08.2017Despatch of a communication from the examining division (Time limit: M04)
19.12.2017Reply to a communication from the examining division
12.04.2018Communication of intention to grant the patent
08.08.2018Fee for grant paid
08.08.2018Fee for publishing/printing paid
08.08.2018Receipt of the translation of the claim(s)
Divisional application(s)The date of the Examining Division's first communication in respect of the earliest application for which a communication has been issued is  28.08.2017
Opposition(s)20.06.2019No opposition filed within time limit [2019/35]
Fees paidRenewal fee
26.09.2016Renewal fee patent year 03
10.03.2017Renewal fee patent year 04
13.03.2018Renewal fee patent year 05
Penalty fee
Additional fee for renewal fee
31.03.201603   M06   Fee paid on   26.09.2016
Opt-out from the exclusive  Tooltip
competence of the Unified
Patent Court		 See the Register of the Unified Patent Court for opt-out data
Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Lapses during opposition  TooltipHU14.03.2014
AL19.09.2018
AT19.09.2018
CY19.09.2018
CZ19.09.2018
DK19.09.2018
EE19.09.2018
ES19.09.2018
FI19.09.2018
HR19.09.2018
IT19.09.2018
LT19.09.2018
LV19.09.2018
MC19.09.2018
MK19.09.2018
NL19.09.2018
PL19.09.2018
RO19.09.2018
RS19.09.2018
SE19.09.2018
SI19.09.2018
SK19.09.2018
SM19.09.2018
TR19.09.2018
BG19.12.2018
NO19.12.2018
GR20.12.2018
IS19.01.2019
PT19.01.2019
LU14.03.2019
MT14.03.2019
BE31.03.2019
CH31.03.2019
LI31.03.2019
[2022/31]
Former [2021/32]HU14.03.2014
AL19.09.2018
AT19.09.2018
CY19.09.2018
CZ19.09.2018
DK19.09.2018
EE19.09.2018
ES19.09.2018
FI19.09.2018
HR19.09.2018
IT19.09.2018
LT19.09.2018
LV19.09.2018
MC19.09.2018
NL19.09.2018
PL19.09.2018
RO19.09.2018
RS19.09.2018
SE19.09.2018
SI19.09.2018
SK19.09.2018
SM19.09.2018
TR19.09.2018
BG19.12.2018
NO19.12.2018
GR20.12.2018
IS19.01.2019
PT19.01.2019
LU14.03.2019
MT14.03.2019
BE31.03.2019
CH31.03.2019
LI31.03.2019
Former [2021/26]AL19.09.2018
AT19.09.2018
CY19.09.2018
CZ19.09.2018
DK19.09.2018
EE19.09.2018
ES19.09.2018
FI19.09.2018
HR19.09.2018
IT19.09.2018
LT19.09.2018
LV19.09.2018
MC19.09.2018
NL19.09.2018
PL19.09.2018
RO19.09.2018
RS19.09.2018
SE19.09.2018
SI19.09.2018
SK19.09.2018
SM19.09.2018
TR19.09.2018
BG19.12.2018
NO19.12.2018
GR20.12.2018
IS19.01.2019
PT19.01.2019
LU14.03.2019
MT14.03.2019
BE31.03.2019
CH31.03.2019
LI31.03.2019
Former [2020/29]AL19.09.2018
AT19.09.2018
CZ19.09.2018
DK19.09.2018
EE19.09.2018
ES19.09.2018
FI19.09.2018
HR19.09.2018
IT19.09.2018
LT19.09.2018
LV19.09.2018
MC19.09.2018
NL19.09.2018
PL19.09.2018
RO19.09.2018
RS19.09.2018
SE19.09.2018
SI19.09.2018
SK19.09.2018
SM19.09.2018
TR19.09.2018
BG19.12.2018
NO19.12.2018
GR20.12.2018
IS19.01.2019
PT19.01.2019
LU14.03.2019
MT14.03.2019
BE31.03.2019
CH31.03.2019
LI31.03.2019
Former [2020/17]AL19.09.2018
AT19.09.2018
CZ19.09.2018
DK19.09.2018
EE19.09.2018
ES19.09.2018
FI19.09.2018
HR19.09.2018
IT19.09.2018
LT19.09.2018
LV19.09.2018
MC19.09.2018
NL19.09.2018
PL19.09.2018
RO19.09.2018
RS19.09.2018
SE19.09.2018
SI19.09.2018
SK19.09.2018
SM19.09.2018
TR19.09.2018
BG19.12.2018
NO19.12.2018
GR20.12.2018
IS19.01.2019
PT19.01.2019
LU14.03.2019
BE31.03.2019
CH31.03.2019
LI31.03.2019
Former [2020/11]AL19.09.2018
AT19.09.2018
CZ19.09.2018
DK19.09.2018
EE19.09.2018
ES19.09.2018
FI19.09.2018
HR19.09.2018
IT19.09.2018
LT19.09.2018
LV19.09.2018
MC19.09.2018
NL19.09.2018
PL19.09.2018
RO19.09.2018
RS19.09.2018
SE19.09.2018
SI19.09.2018
SK19.09.2018
SM19.09.2018
BG19.12.2018
NO19.12.2018
GR20.12.2018
IS19.01.2019
PT19.01.2019
LU14.03.2019
BE31.03.2019
CH31.03.2019
LI31.03.2019
Former [2020/01]AL19.09.2018
AT19.09.2018
CZ19.09.2018
DK19.09.2018
EE19.09.2018
ES19.09.2018
FI19.09.2018
HR19.09.2018
IT19.09.2018
LT19.09.2018
LV19.09.2018
MC19.09.2018
NL19.09.2018
PL19.09.2018
RO19.09.2018
RS19.09.2018
SE19.09.2018
SI19.09.2018
SK19.09.2018
SM19.09.2018
BG19.12.2018
NO19.12.2018
GR20.12.2018
IS19.01.2019
PT19.01.2019
LU14.03.2019
Former [2019/50]AL19.09.2018
AT19.09.2018
CZ19.09.2018
DK19.09.2018
EE19.09.2018
ES19.09.2018
FI19.09.2018
HR19.09.2018
IT19.09.2018
LT19.09.2018
LV19.09.2018
MC19.09.2018
NL19.09.2018
PL19.09.2018
RO19.09.2018
RS19.09.2018
SE19.09.2018
SI19.09.2018
SK19.09.2018
SM19.09.2018
BG19.12.2018
NO19.12.2018
GR20.12.2018
IS19.01.2019
PT19.01.2019
Former [2019/46]AL19.09.2018
AT19.09.2018
CZ19.09.2018
DK19.09.2018
EE19.09.2018
ES19.09.2018
FI19.09.2018
HR19.09.2018
IT19.09.2018
LT19.09.2018
LV19.09.2018
MC19.09.2018
NL19.09.2018
PL19.09.2018
RO19.09.2018
RS19.09.2018
SE19.09.2018
SK19.09.2018
SM19.09.2018
BG19.12.2018
NO19.12.2018
GR20.12.2018
IS19.01.2019
PT19.01.2019
Former [2019/34]AL19.09.2018
AT19.09.2018
CZ19.09.2018
DK19.09.2018
EE19.09.2018
ES19.09.2018
FI19.09.2018
HR19.09.2018
IT19.09.2018
LT19.09.2018
LV19.09.2018
NL19.09.2018
PL19.09.2018
RO19.09.2018
RS19.09.2018
SE19.09.2018
SK19.09.2018
SM19.09.2018
BG19.12.2018
NO19.12.2018
GR20.12.2018
IS19.01.2019
PT19.01.2019
Former [2019/26]AL19.09.2018
AT19.09.2018
CZ19.09.2018
EE19.09.2018
ES19.09.2018
FI19.09.2018
HR19.09.2018
IT19.09.2018
LT19.09.2018
LV19.09.2018
NL19.09.2018
PL19.09.2018
RO19.09.2018
RS19.09.2018
SE19.09.2018
SK19.09.2018
SM19.09.2018
BG19.12.2018
NO19.12.2018
GR20.12.2018
IS19.01.2019
PT19.01.2019
Former [2019/23]AL19.09.2018
AT19.09.2018
CZ19.09.2018
EE19.09.2018
ES19.09.2018
FI19.09.2018
HR19.09.2018
IT19.09.2018
LT19.09.2018
LV19.09.2018
NL19.09.2018
PL19.09.2018
RO19.09.2018
RS19.09.2018
SE19.09.2018
SK19.09.2018
BG19.12.2018
NO19.12.2018
GR20.12.2018
IS19.01.2019
Former [2019/22]AL19.09.2018
CZ19.09.2018
FI19.09.2018
HR19.09.2018
IT19.09.2018
LT19.09.2018
LV19.09.2018
NL19.09.2018
PL19.09.2018
RO19.09.2018
RS19.09.2018
SE19.09.2018
BG19.12.2018
NO19.12.2018
GR20.12.2018
IS19.01.2019
Former [2019/21]AL19.09.2018
FI19.09.2018
HR19.09.2018
IT19.09.2018
LT19.09.2018
LV19.09.2018
NL19.09.2018
RS19.09.2018
SE19.09.2018
BG19.12.2018
NO19.12.2018
GR20.12.2018
IS19.01.2019
Former [2019/20]AL19.09.2018
FI19.09.2018
HR19.09.2018
LT19.09.2018
LV19.09.2018
NL19.09.2018
RS19.09.2018
SE19.09.2018
BG19.12.2018
NO19.12.2018
GR20.12.2018
Former [2019/12]AL19.09.2018
FI19.09.2018
HR19.09.2018
LT19.09.2018
LV19.09.2018
RS19.09.2018
SE19.09.2018
BG19.12.2018
NO19.12.2018
GR20.12.2018
Former [2019/11]FI19.09.2018
HR19.09.2018
LT19.09.2018
RS19.09.2018
SE19.09.2018
BG19.12.2018
NO19.12.2018
GR20.12.2018
Former [2019/10]FI19.09.2018
LT19.09.2018
RS19.09.2018
SE19.09.2018
BG19.12.2018
NO19.12.2018
GR20.12.2018
Former [2019/08]FI19.09.2018
LT19.09.2018
NO19.12.2018
Former [2019/07]LT19.09.2018
Documents cited:Search[Y]EP2202522  (UNIV LEIDEN [NL]) [Y] 18* examples; claims *;
 [Y]WO2012168184  (MEDIPAN GMBH [DE], et al) [Y] 1-19 * examples; claims *;
 [Y]  - JENNIFER S DICKEY ET AL, "H2AX: functional roles and potential applications", CHROMOSOMA ; BIOLOGY OF THE NUCLEUS, SPRINGER, BERLIN, DE, (20090826), vol. 118, no. 6, doi:10.1007/S00412-009-0234-4, ISSN 1432-0886, pages 683 - 692, XP019760098 [Y] 1-19 * page 689; "Conclusion" *

DOI:   http://dx.doi.org/10.1007/s00412-009-0234-4
 [Y]  - J. PALMA ET AL, "MicroRNAs are exported from malignant cells in customized particles", NUCLEIC ACIDS RESEARCH, GB, (20120101), vol. 40, no. 18, doi:10.1093/nar/gks656, ISSN 0305-1048, pages 9125 - 9138, XP055292912 [Y] 1-19 * page 9132, column 2 *

DOI:   http://dx.doi.org/10.1093/nar/gks656
 [Y]  - CLINGEN P H ET AL, "Histone H2AX phosphorylation as a molecular pharmacological marker for DNA interstrand crosslink cancer chemotherapy", BIOCHEMICAL PHARMACOLOGY, ELSEVIER, US, vol. 76, no. 1, doi:10.1016/J.BCP.2008.03.025, ISSN 0006-2952, (20080701), pages 19 - 27, (20080416), XP022757456 [Y] 1-19 * abstract; page 26, last sentence *

DOI:   http://dx.doi.org/10.1016/j.bcp.2008.03.025
 [Y]  - L-J MAH ET AL, "[gamma]H2AX: a sensitive molecular marker of DNA damage and repair", LEUKEMIA., US, (20100204), vol. 24, no. 4, doi:10.1038/leu.2010.6, ISSN 0887-6924, pages 679 - 686, XP055326988 [Y] 1-19 * page 682-683 *

DOI:   http://dx.doi.org/10.1038/leu.2010.6
International search[Y]  - DICKEY ET AL., "H2AX: functional roles and potential applications.", CHROMOSOMA, (200912), vol. 118, no. 6, doi:10.1007/s00412-009-0234-4, pages 683 - 692, XP019760098

DOI:   http://dx.doi.org/10.1007/s00412-009-0234-4
 [Y]  - PALMA ET AL., "MicroRNAs are exported from malignant cells in customized particles.", NUCLEIC ACIDS RES., (20120101), vol. 40, no. 18, doi:10.1093/nar/gks656, pages 9125 - 9138, XP055292912

DOI:   http://dx.doi.org/10.1093/nar/gks656
 [Y]  - ZHANG ET AL., "Temozolomide: Mechanisms of action, repair and resistance", CURRENT MOLECULAR PHARMACOLOGY, (20120101), vol. 5, no. 1, doi:10.2174/1874467211205010102, pages 102 - 114, XP055292915

DOI:   http://dx.doi.org/10.2174/1874467211205010102
 [Y]  - POMMIER ET AL., "The indenoisoquinolines noncamptothecin topoisomerase I inhibitors: Update and perspectives", MOLECULAR CANCER THERAPEUTICS, (20090501), vol. 8, no. 5, doi:10.1158/1535-7163.MCT-08-0706, pages 1008 - 1014, XP055292917

DOI:   http://dx.doi.org/10.1158/1535-7163.MCT-08-0706
by applicantUS6812023
 US6899863
    - HARTMANN et al., Lancet, (20100000), vol. 11, pages 383 - 390
    - ANDERSON; ANDERSON, Mol Cell Proteomics, (20020000), vol. 1.11, pages 845 - 867
    - SANCHEZ-CARBAYO, Tumor Biol, (20100000), vol. 31, pages 103 - 112
    - BROWN C., "Antigen retrieval methods for immunohistochemistry", Toxicol Pathol, (19980000), vol. 26, no. 6, pages 830 - 1
    - ONORATO et al., "Immunohistochemical and ELISA assays for biomarkers of oxidative stress in aging and disease", Ann NY Acad Sci, (19980000), vol. 20, no. 854, pages 277 - 90
    - LAEMMELI UK, "Cleavage of structural proteins during the assembly of the head of a bacteriophage T4", Nature, (19700000), vol. 227, pages 680 - 685
    - EGGER; BIENZ, "Protein (western) blotting", Mol Biotechnol, (19940000), vol. 1, no. 3, doi:doi:10.1007/BF02921696, pages 289 - 305, XP001023417

DOI:   http://dx.doi.org/10.1007/BF02921696
    - HUANG, "Detection of multiple proteins in an antibody-based protein microarray system", Immunol Methods, (20010000), vol. 255, no. 1-2, doi:doi:10.1016/S0022-1759(01)00394-5, pages 1 - 13, XP004274816

DOI:   http://dx.doi.org/10.1016/S0022-1759(01)00394-5
    - Methods in Cell Biology: Antibodies in Cell Biology, (19930000), vol. 37
    - COCUCCI et al., Traffic, (20070000), vol. 8, pages 742 - 757
    - SIMPSON et al., Proteomics, (20080000), vol. 8, pages 4083 - 4099
    - RAPOSO et al., J Exp Med, (19960000), vol. 183, pages 1161 - 72
    - TAYLOR; GERCEL-TAYLOR, Gynecol Oncol, (20080000), vol. 110, pages 13 - 21
    - CHERUVANKY et al., Am J Physiol Renal Physiol, (20070000), vol. 292, pages 1657 - 61
    - NAGRATH et al., Nature, (20070000), vol. 450, pages 1235 - 9
    - CABY et al., Inter Immunol, (20050000), vol. 17, no. 7, pages 879 - 887
    - BARD, MP et al., Am JRespir Cell Mol Biol, (20040000), vol. 31, no. 1, pages 114 - 21
    - HEGMANS, JP et al., Am J Pathol, (20040000), vol. 164, no. 5, pages 1807 - 15
    - MEARS, R et al., Proteomics, (20040000), vol. 4, no. 12, pages 4019 - 31
    - CHOI, DS et al., J Proteome Res, (20070000), vol. 6, no. 12, pages 4646 - 55
    - LATERZA et al., Clin Chem, (20060000), vol. 52, no. 9, pages 1713 - 21
    - WUBBOLTS R; LECKIE RS; VEENHUIZEN PT; SCHWARZMANN G; MOBIUS W; HOERNSCHEMEYER J; SLOT JW; GEUZE HJ; STOORVOGEL W, JBiol Chem, (20030328), vol. 278, no. 13, pages 10963 - 72
    - TAYLOR, C.G.; ATAY, S.; TULLIS, R.H.; KESIMER, M; TAYLOR, D.D., Anal. Biochem., (20120000), vol. 28, no. 1, pages 44 - 53
    - BOTELHO et al., J. Proteome Res., (20100000), vol. 9, pages 2863 - 2870
    - YATES et al., Anal. Chem., (20040000), vol. 76, pages 4193 - 4201
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