EP3041937 - SWITCHABLE CAS9 NUCLEASES AND USES THEREOF [Right-click to bookmark this link] | Status | No opposition filed within time limit Status updated on 30.10.2020 Database last updated on 18.11.2024 | |
Former | The patent has been granted Status updated on 22.11.2019 | ||
Former | Grant of patent is intended Status updated on 18.07.2019 | ||
Former | Examination is in progress Status updated on 28.04.2017 | Most recent event Tooltip | 08.07.2022 | Lapse of the patent in a contracting state New state(s): MK | published on 10.08.2022 [2022/32] | Applicant(s) | For all designated states President and Fellows of Harvard College 17 Quincy Street Cambridge, MA 02138 / US | [2016/28] | Inventor(s) | 01 /
LIU, David, R. 3 Whitman Circle Lexington, MA 02420 / US | 02 /
HU, Johnny, Hao 553 Cabot Mail Center 60 Linnaean Street Cambridge, MA 02138 / US | [2016/28] | Representative(s) | Lee, Nicholas John, et al Kilburn & Strode LLP Lacon London 84 Theobalds Road London WC1X 8NL / GB | [2019/52] |
Former [2016/28] | Crooks, Elizabeth Caroline, et al Kilburn & Strode LLP 20 Red Lion Street London WC1R 4PJ / GB | Application number, filing date | 14783690.2 | 05.09.2014 | [2016/28] | WO2014US54252 | Priority number, date | US201361874682P | 06.09.2013 Original published format: US 201361874682 P | US201414326329 | 08.07.2014 Original published format: US201414326329 | US201414326340 | 08.07.2014 Original published format: US201414326340 | US201414326361 | 08.07.2014 Original published format: US201414326361 | [2016/28] | Filing language | EN | Procedural language | EN | Publication | Type: | A2 Application without search report | No.: | WO2015035139 | Date: | 12.03.2015 | Language: | EN | [2015/10] | Type: | A2 Application without search report | No.: | EP3041937 | Date: | 13.07.2016 | Language: | EN | The application published by WIPO in one of the EPO official languages on 12.03.2015 takes the place of the publication of the European patent application. | [2016/28] | Type: | B1 Patent specification | No.: | EP3041937 | Date: | 25.12.2019 | Language: | EN | [2019/52] | Search report(s) | International search report - published on: | EP | 30.04.2015 | Classification | IPC: | C12N15/113, C12N15/63, C12N15/10, C12N15/90 | [2019/31] | CPC: |
A61P43/00 (EP);
C12N15/102 (EP,KR,US);
C12N15/907 (US);
C12N15/01 (EP,US);
C12N15/113 (EP,KR,US);
C12N15/115 (KR,US);
C12N15/63 (EP,KR,US);
C12N9/22 (EP,KR,US);
C12N9/96 (EP,US);
A61K48/00 (US);
C12N2310/10 (US);
C12N2310/12 (EP,US);
C12N2310/16 (EP,KR,US);
C12N2310/20 (KR);
C12N2310/3519 (EP,KR,US);
|
Former IPC [2016/28] | C12N15/113 | Designated contracting states | AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LI, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR [2016/28] | Extension states | BA | Not yet paid | ME | Not yet paid | Title | German: | SCHALTBARE CAS9 NUKLEASEN UND DEREN VERWENDUNG | [2019/31] | English: | SWITCHABLE CAS9 NUCLEASES AND USES THEREOF | [2016/28] | French: | NUCLÉASES CAS9 COMMUTABLES ET LEURS UTILISATIONS | [2016/28] |
Former [2016/28] | UMSCHALTBARE CAS9-NUKLEASEN UND VERWENDUNGEN DAVON | Entry into regional phase | 23.03.2016 | National basic fee paid | 23.03.2016 | Designation fee(s) paid | 23.03.2016 | Examination fee paid | Examination procedure | 23.03.2016 | Examination requested [2016/28] | 18.10.2016 | Amendment by applicant (claims and/or description) | 28.04.2017 | Despatch of a communication from the examining division (Time limit: M06) | 06.11.2017 | Reply to a communication from the examining division | 15.02.2018 | Despatch of a communication from the examining division (Time limit: M04) | 25.06.2018 | Reply to a communication from the examining division | 16.08.2018 | Despatch of a communication from the examining division (Time limit: M06) | 22.02.2019 | Reply to a communication from the examining division | 19.07.2019 | Communication of intention to grant the patent | 15.11.2019 | Fee for grant paid | 15.11.2019 | Fee for publishing/printing paid | 15.11.2019 | Receipt of the translation of the claim(s) | Divisional application(s) | EP19218912.4 / EP3693461 | Opposition(s) | 28.09.2020 | No opposition filed within time limit [2020/49] | Fees paid | Renewal fee | 27.09.2016 | Renewal fee patent year 03 | 27.09.2017 | Renewal fee patent year 04 | 27.09.2018 | Renewal fee patent year 05 | 27.09.2019 | Renewal fee patent year 06 |
Opt-out from the exclusive Tooltip competence of the Unified Patent Court | See the Register of the Unified Patent Court for opt-out data | ||
Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court. | Lapses during opposition Tooltip | HU | 05.09.2014 | AL | 25.12.2019 | AT | 25.12.2019 | CY | 25.12.2019 | CZ | 25.12.2019 | DK | 25.12.2019 | EE | 25.12.2019 | ES | 25.12.2019 | FI | 25.12.2019 | HR | 25.12.2019 | IT | 25.12.2019 | LT | 25.12.2019 | LV | 25.12.2019 | MC | 25.12.2019 | MK | 25.12.2019 | MT | 25.12.2019 | NL | 25.12.2019 | PL | 25.12.2019 | RO | 25.12.2019 | RS | 25.12.2019 | SE | 25.12.2019 | SI | 25.12.2019 | SK | 25.12.2019 | SM | 25.12.2019 | TR | 25.12.2019 | BG | 25.03.2020 | NO | 25.03.2020 | GR | 26.03.2020 | IS | 25.04.2020 | PT | 20.05.2020 | IE | 05.09.2020 | LU | 05.09.2020 | [2022/32] |
Former [2022/27] | HU | 05.09.2014 | |
AL | 25.12.2019 | ||
AT | 25.12.2019 | ||
CY | 25.12.2019 | ||
CZ | 25.12.2019 | ||
DK | 25.12.2019 | ||
EE | 25.12.2019 | ||
ES | 25.12.2019 | ||
FI | 25.12.2019 | ||
HR | 25.12.2019 | ||
IT | 25.12.2019 | ||
LT | 25.12.2019 | ||
LV | 25.12.2019 | ||
MC | 25.12.2019 | ||
MT | 25.12.2019 | ||
NL | 25.12.2019 | ||
PL | 25.12.2019 | ||
RO | 25.12.2019 | ||
RS | 25.12.2019 | ||
SE | 25.12.2019 | ||
SI | 25.12.2019 | ||
SK | 25.12.2019 | ||
SM | 25.12.2019 | ||
TR | 25.12.2019 | ||
BG | 25.03.2020 | ||
NO | 25.03.2020 | ||
GR | 26.03.2020 | ||
IS | 25.04.2020 | ||
PT | 20.05.2020 | ||
IE | 05.09.2020 | ||
LU | 05.09.2020 | ||
Former [2021/37] | AL | 25.12.2019 | |
AT | 25.12.2019 | ||
CZ | 25.12.2019 | ||
DK | 25.12.2019 | ||
EE | 25.12.2019 | ||
ES | 25.12.2019 | ||
FI | 25.12.2019 | ||
HR | 25.12.2019 | ||
IT | 25.12.2019 | ||
LT | 25.12.2019 | ||
LV | 25.12.2019 | ||
MC | 25.12.2019 | ||
NL | 25.12.2019 | ||
PL | 25.12.2019 | ||
RO | 25.12.2019 | ||
RS | 25.12.2019 | ||
SE | 25.12.2019 | ||
SI | 25.12.2019 | ||
SK | 25.12.2019 | ||
SM | 25.12.2019 | ||
BG | 25.03.2020 | ||
NO | 25.03.2020 | ||
GR | 26.03.2020 | ||
IS | 25.04.2020 | ||
PT | 20.05.2020 | ||
IE | 05.09.2020 | ||
LU | 05.09.2020 | ||
Former [2021/27] | AL | 25.12.2019 | |
AT | 25.12.2019 | ||
CZ | 25.12.2019 | ||
DK | 25.12.2019 | ||
EE | 25.12.2019 | ||
ES | 25.12.2019 | ||
FI | 25.12.2019 | ||
HR | 25.12.2019 | ||
IT | 25.12.2019 | ||
LT | 25.12.2019 | ||
LV | 25.12.2019 | ||
MC | 25.12.2019 | ||
NL | 25.12.2019 | ||
PL | 25.12.2019 | ||
RO | 25.12.2019 | ||
RS | 25.12.2019 | ||
SE | 25.12.2019 | ||
SI | 25.12.2019 | ||
SK | 25.12.2019 | ||
SM | 25.12.2019 | ||
BG | 25.03.2020 | ||
NO | 25.03.2020 | ||
GR | 26.03.2020 | ||
IS | 25.04.2020 | ||
PT | 20.05.2020 | ||
LU | 05.09.2020 | ||
Former [2021/23] | AL | 25.12.2019 | |
AT | 25.12.2019 | ||
CZ | 25.12.2019 | ||
DK | 25.12.2019 | ||
EE | 25.12.2019 | ||
ES | 25.12.2019 | ||
FI | 25.12.2019 | ||
HR | 25.12.2019 | ||
IT | 25.12.2019 | ||
LT | 25.12.2019 | ||
LV | 25.12.2019 | ||
MC | 25.12.2019 | ||
NL | 25.12.2019 | ||
PL | 25.12.2019 | ||
RO | 25.12.2019 | ||
RS | 25.12.2019 | ||
SE | 25.12.2019 | ||
SI | 25.12.2019 | ||
SK | 25.12.2019 | ||
SM | 25.12.2019 | ||
BG | 25.03.2020 | ||
NO | 25.03.2020 | ||
GR | 26.03.2020 | ||
IS | 25.04.2020 | ||
PT | 20.05.2020 | ||
Former [2021/10] | AL | 25.12.2019 | |
AT | 25.12.2019 | ||
CZ | 25.12.2019 | ||
DK | 25.12.2019 | ||
EE | 25.12.2019 | ||
ES | 25.12.2019 | ||
FI | 25.12.2019 | ||
HR | 25.12.2019 | ||
IT | 25.12.2019 | ||
LT | 25.12.2019 | ||
LV | 25.12.2019 | ||
NL | 25.12.2019 | ||
PL | 25.12.2019 | ||
RO | 25.12.2019 | ||
RS | 25.12.2019 | ||
SE | 25.12.2019 | ||
SI | 25.12.2019 | ||
SK | 25.12.2019 | ||
SM | 25.12.2019 | ||
BG | 25.03.2020 | ||
NO | 25.03.2020 | ||
GR | 26.03.2020 | ||
IS | 25.04.2020 | ||
PT | 20.05.2020 | ||
Former [2020/51] | AL | 25.12.2019 | |
CZ | 25.12.2019 | ||
DK | 25.12.2019 | ||
EE | 25.12.2019 | ||
ES | 25.12.2019 | ||
FI | 25.12.2019 | ||
HR | 25.12.2019 | ||
LT | 25.12.2019 | ||
LV | 25.12.2019 | ||
NL | 25.12.2019 | ||
RO | 25.12.2019 | ||
RS | 25.12.2019 | ||
SE | 25.12.2019 | ||
SI | 25.12.2019 | ||
SK | 25.12.2019 | ||
SM | 25.12.2019 | ||
BG | 25.03.2020 | ||
NO | 25.03.2020 | ||
GR | 26.03.2020 | ||
IS | 25.04.2020 | ||
PT | 20.05.2020 | ||
Former [2020/47] | AL | 25.12.2019 | |
CZ | 25.12.2019 | ||
DK | 25.12.2019 | ||
EE | 25.12.2019 | ||
ES | 25.12.2019 | ||
FI | 25.12.2019 | ||
HR | 25.12.2019 | ||
LT | 25.12.2019 | ||
LV | 25.12.2019 | ||
NL | 25.12.2019 | ||
RO | 25.12.2019 | ||
RS | 25.12.2019 | ||
SE | 25.12.2019 | ||
SK | 25.12.2019 | ||
SM | 25.12.2019 | ||
BG | 25.03.2020 | ||
NO | 25.03.2020 | ||
GR | 26.03.2020 | ||
IS | 25.04.2020 | ||
PT | 20.05.2020 | ||
Former [2020/40] | AL | 25.12.2019 | |
CZ | 25.12.2019 | ||
EE | 25.12.2019 | ||
FI | 25.12.2019 | ||
HR | 25.12.2019 | ||
LT | 25.12.2019 | ||
LV | 25.12.2019 | ||
NL | 25.12.2019 | ||
RO | 25.12.2019 | ||
RS | 25.12.2019 | ||
SE | 25.12.2019 | ||
SK | 25.12.2019 | ||
SM | 25.12.2019 | ||
BG | 25.03.2020 | ||
NO | 25.03.2020 | ||
GR | 26.03.2020 | ||
IS | 25.04.2020 | ||
PT | 20.05.2020 | ||
Former [2020/37] | AL | 25.12.2019 | |
CZ | 25.12.2019 | ||
EE | 25.12.2019 | ||
FI | 25.12.2019 | ||
HR | 25.12.2019 | ||
LT | 25.12.2019 | ||
LV | 25.12.2019 | ||
NL | 25.12.2019 | ||
RO | 25.12.2019 | ||
RS | 25.12.2019 | ||
SE | 25.12.2019 | ||
SK | 25.12.2019 | ||
SM | 25.12.2019 | ||
BG | 25.03.2020 | ||
NO | 25.03.2020 | ||
GR | 26.03.2020 | ||
PT | 20.05.2020 | ||
Former [2020/36] | AL | 25.12.2019 | |
CZ | 25.12.2019 | ||
EE | 25.12.2019 | ||
FI | 25.12.2019 | ||
HR | 25.12.2019 | ||
LT | 25.12.2019 | ||
LV | 25.12.2019 | ||
NL | 25.12.2019 | ||
RO | 25.12.2019 | ||
RS | 25.12.2019 | ||
SE | 25.12.2019 | ||
BG | 25.03.2020 | ||
NO | 25.03.2020 | ||
GR | 26.03.2020 | ||
PT | 20.05.2020 | ||
Former [2020/35] | AL | 25.12.2019 | |
CZ | 25.12.2019 | ||
FI | 25.12.2019 | ||
HR | 25.12.2019 | ||
LT | 25.12.2019 | ||
LV | 25.12.2019 | ||
NL | 25.12.2019 | ||
RO | 25.12.2019 | ||
RS | 25.12.2019 | ||
SE | 25.12.2019 | ||
BG | 25.03.2020 | ||
NO | 25.03.2020 | ||
GR | 26.03.2020 | ||
Former [2020/32] | AL | 25.12.2019 | |
FI | 25.12.2019 | ||
HR | 25.12.2019 | ||
LT | 25.12.2019 | ||
LV | 25.12.2019 | ||
NL | 25.12.2019 | ||
RS | 25.12.2019 | ||
SE | 25.12.2019 | ||
BG | 25.03.2020 | ||
NO | 25.03.2020 | ||
GR | 26.03.2020 | ||
Former [2020/25] | FI | 25.12.2019 | |
HR | 25.12.2019 | ||
LT | 25.12.2019 | ||
LV | 25.12.2019 | ||
RS | 25.12.2019 | ||
SE | 25.12.2019 | ||
BG | 25.03.2020 | ||
NO | 25.03.2020 | ||
GR | 26.03.2020 | ||
Former [2020/23] | FI | 25.12.2019 | |
LT | 25.12.2019 | ||
LV | 25.12.2019 | ||
SE | 25.12.2019 | ||
BG | 25.03.2020 | ||
NO | 25.03.2020 | ||
GR | 26.03.2020 | ||
Former [2020/22] | FI | 25.12.2019 | |
LT | 25.12.2019 | ||
SE | 25.12.2019 | ||
BG | 25.03.2020 | ||
NO | 25.03.2020 | ||
Former [2020/21] | NO | 25.03.2020 | Cited in | International search | [Y]US6057153 (GEORGE SHAJI T [US], et al) [Y] 1-39 * the whole document *; | [Y]WO2006002547 (UNIV SHERBROOKE [CA], et al) [Y] 40-113 * the whole document *; | [Y]WO2006042112 (CALIFORNIA INST OF TECHN [US], et al) [Y] 1-39 * the whole document *; | [Y]US2009234109 (HAN SI-PING [US], et al) [Y] 1-113 * the whole document *; | [AP]WO2013176772 (UNIV CALIFORNIA [US], et al) [AP] 1-39* paragraphs [0395] , [ 426] *; | [IY] - PRASHANT MALI ET AL, "CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering", NATURE BIOTECHNOLOGY, (20130801), vol. 31, no. 9, doi:10.1038/nbt.2675, ISSN 1087-0156, pages 833 - 838, XP055149275 [I] 1-113 * the whole document * [Y] 1-113 DOI: http://dx.doi.org/10.1038/nbt.2675 | [Y] - L. QI ET AL, "Engineering naturally occurring trans-acting non-coding RNAs to sense molecular signals", NUCLEIC ACIDS RESEARCH, (20120301), vol. 40, no. 12, doi:10.1093/nar/gks168, ISSN 0305-1048, pages 5775 - 5786, XP055171547 [Y] 1-39 * the whole document * DOI: http://dx.doi.org/10.1093/nar/gks168 | [Y] - AN CHUNG-IL ET AL, "Artificial control of gene expression in mammalian cells by modulating RNA interference through aptamer-small molecule interaction", RNA, COLD SPRING HARBOR LABORATORY PRESS, US, (20060410), vol. 12, no. 5, doi:10.1261/RNA.2299306, ISSN 1355-8382, pages 710 - 716, XP002514466 [Y] 1-39 * the whole document * DOI: http://dx.doi.org/10.1261/RNA.2299306 | [Y] - YOKO NOMURA ET AL, "Synthetic mammalian riboswitches based on guanine aptazyme", CHEMICAL COMMUNICATIONS, (20120530), vol. 48, no. 57, doi:10.1039/c2cc33140c, ISSN 1359-7345, page 7215, XP055131142 [Y] 1-39 * the whole document * DOI: http://dx.doi.org/10.1039/c2cc33140c | [Y] - B. KLAUSER ET AL, "An engineered small RNA-mediated genetic switch based on a ribozyme expression platform", NUCLEIC ACIDS RESEARCH, (20130412), vol. 41, no. 10, doi:10.1093/nar/gkt253, ISSN 0305-1048, pages 5542 - 5552, XP055171545 [Y] 40-113 * the whole document * DOI: http://dx.doi.org/10.1093/nar/gkt253 | [A] - M. JINEK ET AL, "A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity", SCIENCE, (20120817), vol. 337, no. 6096, doi:10.1126/science.1225829, ISSN 0036-8075, pages 816 - 821, XP055067740 [A] 1-113 * the whole document * DOI: http://dx.doi.org/10.1126/science.1225829 | [A] - LEI S. QI ET AL, "Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression", CELL, (20130228), vol. 152, no. 5, doi:10.1016/j.cell.2013.02.022, ISSN 0092-8674, pages 1173 - 1183, XP055068548 [A] 1-113 * the whole document * DOI: http://dx.doi.org/10.1016/j.cell.2013.02.022 | by applicant | US5580737 | US6453242 | US6503717 | US6534261 | US6599692 | US6607882 | US6689558 | US6824978 | US6933113 | US6979539 | US7013219 | US7163824 | WO2011053982 | US8492082 | - ELLINGTON; SZOSTAK, "In vitro selection of RNA molecules that bind specific ligands", NATURE, (1990), vol. 346, doi:doi:10.1038/346818a0, pages 818 - 822, XP002547962 DOI: http://dx.doi.org/10.1038/346818a0 | - TUERK; GOLD, "Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase", SCIENCE, (1990), vol. 249, pages 05 - 10 | - BURKE; GOLD, "RNA aptamers to the adenosine moiety of S-adenosyl methionine: structural inferences from variations on a theme and the reproducibility of SELEX", NUCLEIC ACIDS RES., (1997), vol. 25, no. 10, doi:doi:10.1093/nar/25.10.2020, pages 2020 - 4, XP002952167 DOI: http://dx.doi.org/10.1093/nar/25.10.2020 | - ULRICH ET AL., "DNA and RNA aptamers: from tools for basic research towards therapeutic applications", COMB CHEM HIGH THROUGHPUT SCREEN, (2006), vol. 9, no. 8, doi:doi:10.2174/138620706778249695, pages 619 - 32, XP008100029 DOI: http://dx.doi.org/10.2174/138620706778249695 | - SVOBODOVA ET AL., "Comparison of different methods for generation of single-stranded DNA for SELEX processes", ANAL BIOANAL CHEM., (2012), vol. 404, doi:doi:10.1007/s00216-012-6183-4, pages 835 - 842, XP035091549 DOI: http://dx.doi.org/10.1007/s00216-012-6183-4 | - DIXON ET AL., "Reengineering orthogonally selective riboswitches", PNAS, (2010), vol. 107, no. 7, doi:doi:10.1073/pnas.0911209107, pages 2830 - 2835, XP055034292 DOI: http://dx.doi.org/10.1073/pnas.0911209107 | - NAHVI ET AL., "Coenzyme B12 riboswitches are widespread genetic control elements in prokaryotes", NUCLEIC ACIDS RES., (2004), vol. 32, pages 143 - 150 | - VITRESCHAK ET AL., "Regulation of the vitamin B12 metabolism and transport in bacteria by a conserved RNA structural element", RNA, (2003), vol. 9, pages 1084 - 1097 | - SUDARSAN ET AL., "Riboswitches in eubacteria sense the second messenger cyclic di-GMP", SCIENCE, (2008), vol. 321, no. 5887, pages 411 - 3 | - LEE ET AL., "An allosteric self-splicing ribozyme triggered by a bacterial second messenger", SCIENCE, (2010), vol. 329, no. 5993, doi:doi:10.1126/science.1190713, pages 845 - 8, XP055010727 DOI: http://dx.doi.org/10.1126/science.1190713 | - WINKLER ET AL., "An mRNA structure that controls gene expression by binding FMN", PROC NATL ACAD SCI USA., (2002), vol. 99, no. 25, doi:doi:10.1073/pnas.212628899, pages 15908 - 15913, XP002374934 DOI: http://dx.doi.org/10.1073/pnas.212628899 | - SERGANOV ET AL., "Coenzyme recognition and gene regulation by a flavin mononucleotide riboswitch", NATURE, (2009), vol. 458, no. 7235, pages 233 - 7 | - WINKLER ET AL., "Control of gene expression by a natural metabolite-responsive ribozyme", NATURE, (2004), vol. 428, doi:doi:10.1038/nature02362, pages 281 - 286, XP002410184 DOI: http://dx.doi.org/10.1038/nature02362 | - JANSEN ET AL., "Backbone and nucleobase contacts to glucosamine-6-phosphate in the glmS ribozyme", NAT STRUCT MOL BIOL., (2006), vol. 13, pages 517 - 523 | - HAMPEL; TINSLEY, "Evidence for preorganization of the glmS ribozyme ligand binding pocket", BIOCHEMISTRY, (2006), vol. 45, pages 7861 - 7871 | - MANDAL ET AL., "A glycine-dependent riboswitch that uses cooperative binding to control gene expression", SCIENCE, (2004), vol. 306, no. 5694, doi:doi:10.1126/science.1100829, pages 275 - 279, XP003000209 DOI: http://dx.doi.org/10.1126/science.1100829 | - KWON; STROBEL, "Chemical basis of glycine riboswitch cooperativity", RNA, (2008), vol. 14, no. 1, pages 25 - 34 | - SUDARSAN ET AL., "An mRNA structure in bacteria that controls gene expression by binding lysine", GENES DEV., (2003), vol. 17, doi:doi:10.1101/GAD.1140003, pages 2688 - 2697, XP008104338 DOI: http://dx.doi.org/10.1101/GAD.1140003 | - GRUNDY ET AL., "The L box regulon: Lysine sensing by leader RNAs of bacterial lysine biosynthesis genes", PROC. NATL. ACAD. SCI. USA., (2003), vol. 100, pages 12057 - 12062 | - ROTH ET AL., "A riboswitch selective for the queuosine precursor preQl contains an unusually small aptamer domain", NAT STRUCT MOL BIOL., (2007), vol. 14, no. 4, pages 308 - 317 | - KLEIN ET AL., "Cocrystal structure of a class I preQl riboswitch reveals a pseudoknot recognizing an essential hypermodified nucleobase", NAT. STRUCT. MOL. BIOL., (2009), vol. 16, no. 3, pages 343 - 344 | - KANG ET AL., "Structural Insights into riboswitch control of the biosynthesis of queuosine, a modified nucleotide found in the anticodon of tRNA", MOL. CELL, (2009), vol. 33, no. 6, pages 784 - 90 | - MEYE ET AL., "Confirmation of a second natural preQl aptamer class in Streptococcaceae bacteria", RNA, (2008), vol. 14, no. 4, doi:doi:10.1261/rna.937308, page 685, XP007911582 DOI: http://dx.doi.org/10.1261/rna.937308 | - SERGANOV ET AL., "Structural basis for discriminative regulation of gene expression by adenine- and guanine-sensing mRNAs", CHEM BIOL., (2004), vol. 11, no. 12, doi:doi:10.1016/j.chembiol.2004.11.018, pages 1729 - 41, XP004689697 DOI: http://dx.doi.org/10.1016/j.chembiol.2004.11.018 | - BATEY ET AL., "Structure of a natural guanine-responsive riboswitch complexed with the metabolite hypoxanthine", NATURE, (2004), vol. 432, no. 7015, pages 411 - 415, XP002577595 | - MANDAL; BREAKER, "Adenine riboswitches and gene activation by disruption of a transcription terminator", NAT STRUCT MOL BIOL., (2004), vol. 11, no. 1, doi:doi:10.1038/NSMB710, pages 29 - 35, XP002577594 DOI: http://dx.doi.org/10.1038/NSMB710 | - WANG ET AL., "Riboswitches that Sense S-adenosylhomocysteine and Activate Genes Involved in Coenzyme Recycling", MOL. CELL, (2008), vol. 29, no. 6, doi:doi:10.1016/J.MOLCEL.2008.01.012, pages 691 - 702, XP002667732 DOI: http://dx.doi.org/10.1016/J.MOLCEL.2008.01.012 | - EDWARDS ET AL., "Structural basis for recognition of S-adenosylhomocysteine by riboswitches", RNA, (2010), vol. 16, no. 11, pages 2144 - 2155 | - MONTANGE ET AL., "Structure of the S-adenosyl methionine riboswitch regulatory mRNA element", NATURE, (2006), vol. 441, pages 1172 - 1175 | - WINKLER ET AL., "An mRNA structure that controls gene expression by binding Sadenosylmethionine", NAT STRUCT BIOL., (2003), vol. 10, pages 701 - 707 | - ZASHA ET AL., "The aptamer core of SAM-IV riboswitches mimics the ligand-binding site of SAM-I riboswitches", RNA, (2008), vol. 14, no. 5, doi:doi:10.1261/rna.988608, pages 822 - 828, XP009134087 DOI: http://dx.doi.org/10.1261/rna.988608 | - AMES ET AL., "A eubacterial riboswitch class that senses the coenzyme tetrahydrofolate", CHEM. BIOL., (2010), vol. 17, no. 7, pages 681 - 5 | - HUANG ET AL., "Long-range pseudoknot interactions dictate the regulatory response in the tetrahydrofolate riboswitch", PROC. NATL. ACAD. SCI. U.S.A., (2011), vol. 108, no. 36, pages 14801 - 6 | - TRAUSCH ET AL., "The structure of a tetrahydrofolate-sensing riboswitch reveals two ligand binding sites in a single aptamer", STRUCTURE, (2011), vol. 19, no. 10, doi:doi:10.1016/j.str.2011.06.019, pages 1413 - 23, XP028320369 DOI: http://dx.doi.org/10.1016/j.str.2011.06.019 | - JENISON ET AL., "High-resolution molecular discrimination by RNA", SCIENCE, (1994), vol. 263, doi:doi:10.1126/science.7510417, pages 1425 - 1429, XP000567866 DOI: http://dx.doi.org/10.1126/science.7510417 | - ZIMMERMAN ET AL., "Molecular interactions and metal binding in the theophylline-binding core of an RNA aptamer", RNA, (2000), vol. 6, no. 5, doi:doi:10.1017/S1355838200000169, pages 659 - 67, XP008004582 DOI: http://dx.doi.org/10.1017/S1355838200000169 | - SUESS ET AL., "A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo", NUCLEIC ACIDS RES., (2004), vol. 32, no. 4, doi:doi:10.1093/NAR/GKH321, pages 1610 - 1614, XP002514467 DOI: http://dx.doi.org/10.1093/NAR/GKH321 | - EDWARDS ET AL., "Crystal structures of the thi-box riboswitch bound to thiamine pyrophosphate analogs reveal adaptive RNA-small molecule recognition", STRUCTURE, (2006), vol. 14, no. 9, doi:doi:10.1016/j.str.2006.07.008, pages 1459 - 68, XP025133992 DOI: http://dx.doi.org/10.1016/j.str.2006.07.008 | - WINKLER ET AL., "Thiamine derivatives bind messenger RNAs directly to regulate bacterial gene expression", NATURE, (2002), vol. 419, no. 6910, doi:doi:10.1038/nature01145, pages 952 - 956, XP002441989 DOI: http://dx.doi.org/10.1038/nature01145 | - SERGANOV ET AL., "Structural basis for gene regulation by a thiamine pyrophosphate-sensing riboswitch", NATURE, (2006), vol. 441, no. 7097, doi:doi:10.1038/nature04740, pages 1167 - 1171, XP002557458 DOI: http://dx.doi.org/10.1038/nature04740 | - JINEK M.; CHYLINSKI K.; FONFARA I.; HAUER M.; DOUDNA J.A.; CHARPENTIER E., SCIENCE, (2012), vol. 337, pages 816 - 821 | - FERRETTI J.J.; MCSHAN W.M.; AJDIC D.J.; SAVIC D.J.; SAVIC G.; LYON K.; PRIMEAUX C.; SEZATE S.; SUVOROV A.N.; KENTON S., "Complete genome sequence of an M1 strain of Streptococcus pyogenes", PROC. NATL. ACAD. SCI. U.S.A., (2001), vol. 98, doi:doi:10.1073/pnas.071559398, pages 4658 - 4663, XP002344854 DOI: http://dx.doi.org/10.1073/pnas.071559398 | - DELTCHEVA E.; CHYLINSKI K.; SHARMA C.M.; GONZALES K.; CHAO Y; PIRZADA Z.A.; ECKERT M.R.; VOGEL J.; CHARPENTIER E., "CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III", NATURE, (2011), vol. 471, doi:doi:10.1038/nature09886, pages 602 - 607, XP055308803 DOI: http://dx.doi.org/10.1038/nature09886 | - JINEK M.; CHYLINSKI K.; FONFARA I.; HAUER M.; DOUDNA J.A.; CHARPENTIER E., "A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity", SCIENCE, (2012), vol. 337, doi:doi:10.1126/science.1225829, pages 816 - 821, XP055299674 DOI: http://dx.doi.org/10.1126/science.1225829 | - CHYLINSKI; RHUN; CHARPENTIER, "The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems", RNA BIOLOGY, (2013), vol. 10, no. 5, doi:doi:10.4161/rna.24321, pages 726 - 737, XP055116068 DOI: http://dx.doi.org/10.4161/rna.24321 | - JINEK ET AL., SCIENCE, (2012), vol. 337, pages 816 - 821 | - FERRETTI J.J.; MCSHAN W.M.; AJDIC D.J.; SAVIC D.J.; SAVIC G.; LYON K.; PRIMEAUX C.; SEZATE S.; SUVOROV A.N.; KENTON S., "Complete genome sequence of an M strain of Streptococcus pyogenes", PROC. NATL. ACAD. SCI. U.S.A., (2001), vol. 98, doi:doi:10.1073/pnas.071559398, pages 4658 - 4663, XP002344854 DOI: http://dx.doi.org/10.1073/pnas.071559398 | - DELTCHEVA E.; CHYLINSKI K.; SHARMA C.M.; GONZALES K.; CHAO Y.; PIRZADA Z.A.; ECKERT M.R.; VOGEL J.; CHARPENTIER E., "CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III", NATURE, (2011), vol. 471, doi:doi:10.1038/nature09886, pages 602 - 607, XP055308803 DOI: http://dx.doi.org/10.1038/nature09886 | - CONG, L. ET AL., "Multiplex genome engineering using CRISPR/Cas systems", SCIENCE, (2013), vol. 339, doi:doi:10.1126/science.1231143, pages 819 - 823, XP055469277 DOI: http://dx.doi.org/10.1126/science.1232033 | - MALI, P. ET AL., "RNA-guided human genome engineering via Cas9", SCIENCE, (2013), vol. 339, pages 823 - 826 | - HWANG, W.Y. ET AL., "Efficient genome editing in zebrafish using a CRISPR-Cas system", NATURE BIOTECHNOLOGY, (2013), vol. 31, doi:doi:10.1038/nbt.2501, pages 227 - 229, XP055086625 DOI: http://dx.doi.org/10.1038/nbt.2501 | - JINEK, M. ET AL., "RNA-programmed genome editing in human cells", ELIFE, (2013), vol. 2, doi:doi:10.7554/eLife.00471, page E00471, XP055167481 DOI: http://dx.doi.org/10.7554/eLife.00471 | - JIANG, W. ET AL., "RNA-guided editing of bacterial genomes using CRISPR-Cas systems", NATURE BIOTECHNOLOGY, (2013), vol. 31, doi:doi:10.1038/nbt.2508, pages 233 - 239, XP055249123 DOI: http://dx.doi.org/10.1038/nbt.2508 | - ESVELT; WANG, MOLECULAR SYSTEMS BIOLOGY, (2013), vol. 9, page 641 | - PEREZ, E.E. ET AL., "Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases", NATURE BIOTECHNOLOGY, (2008), vol. 26, doi:doi:10.1038/nbt1410, pages 808 - 816, XP055024363 DOI: http://dx.doi.org/10.1038/nbt1410 | - JINEK, M. ET AL., "RNA-programmed genome editing in human cells. eLife 2, e00471 (2013)) and organisms (Hwang, W.Y. et al. Efficient genome editing in zebrafish using a CRISPR-Cas system", NATURE BIOTECHNOLOGY, (2013), vol. 31, pages 227 - 229 | - HWANG, W.Y. ET AL., NATURE BIOTECHNOLOGY, (2013), vol. 31, pages 227 - 229 |