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Extract from the Register of European Patents

EP About this file: EP3325627

EP3325627 - RECOVERIN AS A FUSION PROTEIN TAG TO IMPROVE EXPRESSION, SOLUBILITY AND PURIFICATION OF PROTEINS [Right-click to bookmark this link]
StatusThe application is deemed to be withdrawn
Status updated on  20.11.2020
Database last updated on 03.09.2024
FormerExamination is in progress
Status updated on  16.03.2020
FormerRequest for examination was made
Status updated on  27.04.2018
FormerThe international publication has been made
Status updated on  27.01.2017
Most recent event   Tooltip20.11.2020Application deemed to be withdrawnpublished on 23.12.2020  [2020/52]
Applicant(s)For all designated states
Université Laval
2320 rue des Bibliothèques Local 1434
Québec, QC G1V 0A6 / CA
[2018/22]
Inventor(s)01 / SALESSE, Christian
Département d'ophtalmologie
Université Laval CUO-Recherche
Centre de recherche du CHU de Québec
Hôpital St-Sacrement, 1050 Chemin Ste-Foy
Québec, Québec G1S 4L8 / CA
02 / CANTIN, Line
CUO-Recherche
Centre de recherche du CHU de Québec
Hôpital St-Sacrement
1050 Chemin Ste-Foy
Québec, Québec G1S 4L8 / CA
 [2018/22]
Representative(s)Novagraaf Technologies
Bâtiment O2
2, rue Sarah Bernhardt
CS90017
92665 Asnières-sur-Seine Cedex / FR
[2018/22]
Application number, filing date16826963.719.07.2016
[2018/22]
WO2016CA50849
Priority number, dateUS201562194348P20.07.2015         Original published format: US 201562194348 P
[2018/22]
Filing languageEN
Procedural languageEN
PublicationType: A1 Application with search report
No.:WO2017011909
Date:26.01.2017
Language:EN
[2017/04]
Type: A1 Application with search report 
No.:EP3325627
Date:30.05.2018
Language:EN
The application published by WIPO in one of the EPO official languages on 26.01.2017 takes the place of the publication of the European patent application.
[2018/22]
Search report(s)International search report - published on:CA26.01.2017
(Supplementary) European search report - dispatched on:EP17.04.2019
ClassificationIPC:C12N15/62, C07K1/22, C07K14/47, C07K19/00, C12N15/12, C12N15/63, C12P21/02
[2018/22]
CPC:
C07K14/4728 (EP,US); C07K1/13 (EP,US); C07K1/22 (EP,US);
C07K14/47 (EP,US); C12N9/1029 (EP,US); C12Y203/01135 (EP,US);
C07K2319/00 (EP,US); C07K2319/20 (US) (-)
Designated contracting statesAL,   AT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   LV,   MC,   MK,   MT,   NL,   NO,   PL,   PT,   RO,   RS,   SE,   SI,   SK,   SM,   TR [2018/22]
TitleGerman:RECOVERIN ALS FUSIONSPROTEINTAG ZUR VERBESSERUNG DER EXPRESSION, LÖSLICHKEIT UND REINIGUNG VON PROTEINEN[2018/22]
English:RECOVERIN AS A FUSION PROTEIN TAG TO IMPROVE EXPRESSION, SOLUBILITY AND PURIFICATION OF PROTEINS[2018/22]
French:RECOVERINE UTILISÉE COMME MARQUEUR DE PROTÉINE HYBRIDE DESTINÉ À AMÉLIORER L'EXPRESSION, LA SOLUBILITÉ ET LA PURIFICATION DE PROTÉINES[2018/22]
Entry into regional phase23.01.2018National basic fee paid 
23.01.2018Search fee paid 
23.01.2018Designation fee(s) paid 
23.01.2018Examination fee paid 
Examination procedure23.01.2018Examination requested  [2018/22]
23.01.2018Date on which the examining division has become responsible
27.06.2019Amendment by applicant (claims and/or description)
19.03.2020Despatch of a communication from the examining division (Time limit: M04)
30.07.2020Application deemed to be withdrawn, date of legal effect  [2020/52]
19.08.2020Despatch of communication that the application is deemed to be withdrawn, reason: reply to the communication from the examining division not received in time  [2020/52]
Fees paidRenewal fee
27.07.2018Renewal fee patent year 03
30.07.2019Renewal fee patent year 04
Penalty fee
Additional fee for renewal fee
31.07.202005   M06   Not yet paid
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Documents cited:Search[X]EP1238982  (RIKEN [JP]) [X] 1,2,6-8,11,15 * paragraph [0009] * * paragraph [0013] - paragraph [0016]; figure 1 * * paragraph [0044] - paragraph [0070] *;
 [X]EP2612864  (UNIV DUBLIN [IE]) [X] 1,2,6-8,11,15 * paragraph [0018] - paragraph [0019] * * paragraph [0047] - paragraph [0058] * * paragraph [0012] * * claims 7,12,13,15 *;
 [A]  - Michelle E. Kimple ET AL, "Overview of Affinity Tags for Protein Purification : Affinity Tags for Protein Purification", Current Protocols in Protein Science, Hoboken, NJ, USA, John Wiley & Sons, Inc., (20130924), pages 9.9.1 - 9.9.23, doi:10.1002/0471140864.ps0909s73, ISBN 978-0-471-14086-3, XP055509204 [A] 1-15 * the whole document *

DOI:   http://dx.doi.org/10.1002/0471140864.ps0909s73
 [T]  - BERNIER SARAH C ET AL, "Systematic analysis of the expression, solubility and purification of a passenger protein in fusion with different tags", PROTEIN EXPRESSION AND PURIFICATION, (20180720), vol. 152, doi:10.1016/J.PEP.2018.07.007, ISSN 1046-5928, pages 92 - 106, XP085472410 [T] 1-15 * abstract *

DOI:   http://dx.doi.org/10.1016/j.pep.2018.07.007
International search[XY]WO2009046520  (UNIV HEALTH NETWORK [CA], et al) [X] 1, 2, 12-15, 18-22, 24-27, 29-36, 42-44, 46-49 *Figure 1, [0004-0011], [0069], [0070] * [Y] 3-11, 16, 17, 23, 28, 37-41, 45;
 [X]US5753522  (POLANS ARTHUR S [US], et al) [X] 1-11, 16, 21, 22, 24-27, 29-31, 33-40, 42 *SEQ ID NO: 1, Example 1, claims 4 and 5 *;
 [Y]  - DESMEULES, P. et al., "Single-step purification of myristoylated and nonmyristoylated recoverin and substrate dependence of myristoylation level''.", Analytical Biochemistry, (20060201), vol. 349, no. 1, ISSN 00032697, pages 25 - 32, XP024941967 [Y] 3-11, 16, 17, 23, 28, 37-41, 45 *Materials and Methods*

DOI:   http://dx.doi.org/10.1016/j.ab.2005.11.023
 [A]  - KAWASAKI, H. et al., "Classification and evolution of EF-hand proteins''.", BioMetals, (19981200), vol. 11, no. 4, ISSN 09660844, pages 277 - 295, XP002402107 [A] 1-49 *Whole document *

DOI:   http://dx.doi.org/10.1023/A:1009282307967
The EPO accepts no responsibility for the accuracy of data originating from other authorities; in particular, it does not guarantee that it is complete, up to date or fit for specific purposes.