blank Quick help
blank Maintenance news

Scheduled maintenance

Regular maintenance outages:
between 05.00 and 05.15 hrs CET (Monday to Sunday).

Other outages
Availability

2022.02.11

More...
blank News flashes

News Flashes

New version of the European Patent Register – SPC proceedings information in the Unitary Patent Register.

2024-07-24

More...
blank Related links

Release notes
European Patent Register Assistant
European Patent Register Resource Book
European Patent Register – pocket guide
FAQ - European Patent Register
Federated Register (Flyer)
Global Dossier (Flyer)
Register events
Deep link coverage
Federated Register content
The Patent Information Tour
Fair use charter

Extract from the Register of European Patents

EP About this file: EP3354737

EP3354737 - CELL-FREE PROTEIN SYNTHESIS SYSTEM [Right-click to bookmark this link]
StatusNo opposition filed within time limit
Status updated on  25.06.2021
Database last updated on 05.10.2024
FormerThe patent has been granted
Status updated on  17.07.2020
FormerGrant of patent is intended
Status updated on  05.04.2020
FormerExamination is in progress
Status updated on  29.07.2019
FormerRequest for examination was made
Status updated on  08.02.2019
FormerThe application has been published
Status updated on  29.06.2018
Most recent event   Tooltip13.09.2024Lapse of the patent in a contracting state
New state(s): MT		published on 16.10.2024 [2024/42]
Applicant(s)For all designated states
Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.
Hansastraße 27c
80686 München / DE
[2018/31]
Inventor(s)01 / Buntru, Matthias
Ludwigsallee 131
52062 Aachen / DE
02 / Vogel, Simon
Kronenberg 90
52074 Aachen / DE
03 / Schillberg, Stefan
Dreiländerweg 107
52074 Aachen / DE
 [2018/31]
Representative(s)Roth, Andy Stefan
Dr. Roth Patentanwaltskanzlei
Kaistrasse 5
40221 Düsseldorf / DE
[2018/31]
Application number, filing date17153005.825.01.2017
[2018/31]
Filing languageEN
Procedural languageEN
PublicationType: A1 Application with search report 
No.:EP3354737
Date:01.08.2018
Language:EN
[2018/31]
Type: B1 Patent specification 
No.:EP3354737
Date:19.08.2020
Language:EN
[2020/34]
Search report(s)(Supplementary) European search report - dispatched on:EP03.04.2017
ClassificationIPC:C12N15/82, C12P21/00, C12P21/02
[2018/31]
CPC:
C12N15/8257 (EP,US); A01N3/00 (US); C12P21/02 (EP,US)
Designated contracting statesAL,   AT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   LV,   MC,   MK,   MT,   NL,   NO,   PL,   PT,   RO,   RS,   SE,   SI,   SK,   SM,   TR [2019/11]
Former [2018/31]AL,  AT,  BE,  BG,  CH,  CY,  CZ,  DE,  DK,  EE,  ES,  FI,  FR,  GB,  GR,  HR,  HU,  IE,  IS,  IT,  LI,  LT,  LU,  LV,  MC,  MK,  MT,  NL,  NO,  PL,  PT,  RO,  RS,  SE,  SI,  SK,  SM,  TR 
TitleGerman:SYSTEM ZUR ZELLFREIEN PROTEINSYNTHESE[2018/31]
English:CELL-FREE PROTEIN SYNTHESIS SYSTEM[2018/31]
French:SYSTÈME DE SYNTHÈSE DE PROTÉINE ACELLULAIRE[2018/31]
Examination procedure31.01.2019Amendment by applicant (claims and/or description)
31.01.2019Examination requested  [2019/11]
31.01.2019Date on which the examining division has become responsible
01.08.2019Despatch of a communication from the examining division (Time limit: M04)
11.11.2019Reply to a communication from the examining division
06.04.2020Communication of intention to grant the patent
14.07.2020Fee for grant paid
14.07.2020Fee for publishing/printing paid
14.07.2020Receipt of the translation of the claim(s)
Opposition(s)20.05.2021No opposition filed within time limit [2021/30]
Fees paidRenewal fee
30.01.2019Renewal fee patent year 03
23.01.2020Renewal fee patent year 04
Opt-out from the exclusive  Tooltip
competence of the Unified
Patent Court		 See the Register of the Unified Patent Court for opt-out data
Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Lapses during opposition  TooltipHU25.01.2017
AL19.08.2020
CY19.08.2020
CZ19.08.2020
EE19.08.2020
ES19.08.2020
HR19.08.2020
IT19.08.2020
LT19.08.2020
LV19.08.2020
MC19.08.2020
MK19.08.2020
MT19.08.2020
PL19.08.2020
RO19.08.2020
RS19.08.2020
SI19.08.2020
SK19.08.2020
SM19.08.2020
TR19.08.2020
BG19.11.2020
GR20.11.2020
PT21.12.2020
IE25.01.2021
[2024/42]
Former [2024/29]HU25.01.2017
AL19.08.2020
CY19.08.2020
CZ19.08.2020
EE19.08.2020
ES19.08.2020
HR19.08.2020
IT19.08.2020
LT19.08.2020
LV19.08.2020
MC19.08.2020
MK19.08.2020
PL19.08.2020
RO19.08.2020
RS19.08.2020
SI19.08.2020
SK19.08.2020
SM19.08.2020
TR19.08.2020
BG19.11.2020
GR20.11.2020
PT21.12.2020
IE25.01.2021
Former [2024/22]HU25.01.2017
AL19.08.2020
CY19.08.2020
CZ19.08.2020
EE19.08.2020
ES19.08.2020
HR19.08.2020
IT19.08.2020
LT19.08.2020
LV19.08.2020
MC19.08.2020
MK19.08.2020
PL19.08.2020
RO19.08.2020
RS19.08.2020
SI19.08.2020
SK19.08.2020
SM19.08.2020
BG19.11.2020
GR20.11.2020
PT21.12.2020
IE25.01.2021
Former [2023/33]HU25.01.2017
AL19.08.2020
CY19.08.2020
CZ19.08.2020
EE19.08.2020
ES19.08.2020
HR19.08.2020
IT19.08.2020
LT19.08.2020
LV19.08.2020
MC19.08.2020
PL19.08.2020
RO19.08.2020
RS19.08.2020
SI19.08.2020
SK19.08.2020
SM19.08.2020
BG19.11.2020
GR20.11.2020
PT21.12.2020
IE25.01.2021
Former [2023/30]AL19.08.2020
CY19.08.2020
CZ19.08.2020
EE19.08.2020
ES19.08.2020
HR19.08.2020
IT19.08.2020
LT19.08.2020
LV19.08.2020
MC19.08.2020
PL19.08.2020
RO19.08.2020
RS19.08.2020
SI19.08.2020
SK19.08.2020
SM19.08.2020
BG19.11.2020
GR20.11.2020
PT21.12.2020
IE25.01.2021
Former [2022/07]AL19.08.2020
CZ19.08.2020
EE19.08.2020
ES19.08.2020
HR19.08.2020
IT19.08.2020
LT19.08.2020
LV19.08.2020
MC19.08.2020
PL19.08.2020
RO19.08.2020
RS19.08.2020
SI19.08.2020
SK19.08.2020
SM19.08.2020
BG19.11.2020
GR20.11.2020
PT21.12.2020
IE25.01.2021
Former [2021/40]AL19.08.2020
CZ19.08.2020
EE19.08.2020
ES19.08.2020
HR19.08.2020
IT19.08.2020
LT19.08.2020
LV19.08.2020
MC19.08.2020
PL19.08.2020
RO19.08.2020
RS19.08.2020
SI19.08.2020
SK19.08.2020
SM19.08.2020
BG19.11.2020
GR20.11.2020
PT21.12.2020
Former [2021/36]AL19.08.2020
CZ19.08.2020
EE19.08.2020
ES19.08.2020
HR19.08.2020
IT19.08.2020
LT19.08.2020
LV19.08.2020
PL19.08.2020
RO19.08.2020
RS19.08.2020
SI19.08.2020
SK19.08.2020
SM19.08.2020
BG19.11.2020
GR20.11.2020
PT21.12.2020
Former [2021/34]AL19.08.2020
CZ19.08.2020
EE19.08.2020
ES19.08.2020
HR19.08.2020
IT19.08.2020
LT19.08.2020
LV19.08.2020
PL19.08.2020
RO19.08.2020
RS19.08.2020
SK19.08.2020
SM19.08.2020
BG19.11.2020
GR20.11.2020
PT21.12.2020
Former [2021/28]AL19.08.2020
CZ19.08.2020
EE19.08.2020
ES19.08.2020
HR19.08.2020
LT19.08.2020
LV19.08.2020
PL19.08.2020
RO19.08.2020
RS19.08.2020
SK19.08.2020
SM19.08.2020
BG19.11.2020
GR20.11.2020
PT21.12.2020
Former [2021/25]AL19.08.2020
CZ19.08.2020
EE19.08.2020
ES19.08.2020
HR19.08.2020
LT19.08.2020
LV19.08.2020
PL19.08.2020
RO19.08.2020
RS19.08.2020
SM19.08.2020
BG19.11.2020
GR20.11.2020
PT21.12.2020
Former [2021/22]CZ19.08.2020
EE19.08.2020
HR19.08.2020
LT19.08.2020
LV19.08.2020
PL19.08.2020
RO19.08.2020
RS19.08.2020
SM19.08.2020
BG19.11.2020
GR20.11.2020
PT21.12.2020
Former [2021/10]HR19.08.2020
LT19.08.2020
LV19.08.2020
PL19.08.2020
RS19.08.2020
BG19.11.2020
GR20.11.2020
PT21.12.2020
Former [2021/09]LT19.08.2020
BG19.11.2020
GR20.11.2020
PT21.12.2020
Former [2021/07]LT19.08.2020
Documents cited:Search[XY]US2002039771  (PETERS LARS-ERIK [DE], et al) [X] 1,2,4,6-15 * abstract * * paragraph [0013] * * paragraph [0020] * * paragraph [0025] * * paragraph [0036] - paragraph [0038] * * paragraph [0041] * * paragraph [0048] * * paragraph [0051] * [Y] 3;
 [XY]US2003199076  (KUROITA TOSHIHIRO [JP], et al) [X] 1,2,4,6-12,14,15 * abstract * * paragraph [0007] - paragraph [0010] * * paragraph [0013] * * paragraph [0018] - paragraph [0021] * * paragraph [0088] * [Y] 3;
 [XY]WO2016112258  (SUTRO BIOPHARMA INC [US]) [X] 1,2,5-15 * abstract * * paragraph [0007] * * paragraph [0012] * * paragraph [0014] * * paragraph [0019] - paragraph [0021] * * paragraph [0023] * [Y] 3;
 [X]  - SIDESO ODAFE F P ET AL, "The characteristics and stabilization of a caffeine demethylase enzyme complex", INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, BLACKWELL SCIENTIFIC PUBLICATIONS, OXFORD, GB, vol. 36, no. 6, doi:10.1046/J.1365-2621.2001.00496.X, ISSN 0950-5423, (20010801), pages 693 - 698, (20011220), XP008183826 [X] 1,2,4,6-15 * abstract * * Preparation of cell-free extracts; page 694, column r * * Freeze drying; page 694, column r *

DOI:   http://dx.doi.org/10.1046/j.1365-2621.2001.00496.x
 [Y]  - BUNTRU M ET AL, "A versatile coupled cell-free transcription-translation system based on tobacco BY-2 cell lysates", BIOTECHNOLOGY AND BIOENGINEERING 20150501 JOHN WILEY AND SONS INC. USA, (20150501), vol. 112, no. 5, doi:10.1002/BIT.25502, pages 867 - 878, XP002768481 [Y] 3 * abstract *

DOI:   http://dx.doi.org/10.1002/BIT.25502
 [Y]  - MATTHIAS BUNTRU ET AL, "Tobacco BY-2 cell-free lysate: an alternative and highly-productive plant-based in vitro translation system", BMC BIOTECHNOLOGY, BIOMED CENTRAL LTD. LONDON, GB, (20140503), vol. 14, no. 1, doi:10.1186/1472-6750-14-37, ISSN 1472-6750, page 37, XP021184658 [Y] 3 * abstract *

DOI:   http://dx.doi.org/10.1186/1472-6750-14-37
by applicantUS6168931
 US6337191
    - BUNTRU ET AL., BMC BIOTECHNOLOGY, (2014), vol. 14, page 37
    - BUNTRU ET AL., BIOTECHNOLOGY AND BIOENGINEERING, (2015), vol. 112, no. 5, pages 867 - 878
    - GOERKE, A. R. ET AL., "Development of cell-free protein synthesis platforms for disulfide bonded proteins", BIOTECHNOL. BIOENG., (2008), vol. 99, pages 351 - 367
    - KANTER, G. ET AL., "Cell-free production of scFv fusion proteins: An efficient approach for personalized lymphoma vaccines", BLOOD, (2007), vol. 109, doi:doi:10.1182/blood-2006-07-030593, pages 3393 - 3399, XP055235253

DOI:   http://dx.doi.org/10.1182/blood-2006-07-030593
    - STECH, M. ET AL., "Production of functional antibody fragments in a vesicle-based eukaryotic cell-free translation system", J. BIOTECHNOL., (2012), vol. 164, doi:doi:10.1016/j.jbiotec.2012.08.020, pages 220 - 231, XP028990879

DOI:   http://dx.doi.org/10.1016/j.jbiotec.2012.08.020
    - YANG, J. ET AL., "Rapid expression of vaccine proteins for B-cell lymphoma in a cell-free system", BIOTECHNOL. BIOENG., (2005), vol. 89, doi:doi:10.1002/bit.20283, pages 503 - 511, XP002555162

DOI:   http://dx.doi.org/10.1002/bit.20283
    - YIN, G. ET AL., "Aglycosylated antibodies and antibody fragments produced in a scalable in vitro transcription-translation system", MABS, (2012), vol. 4, doi:doi:10.4161/MABS.4.2.19202, pages 217 - 225, XP002711741

DOI:   http://dx.doi.org/10.4161/MABS.4.2.19202
    - ZAWADA, J. F. ET AL., "Microscale to manufacturing scale-up of cell-free cytokine production- a new approach for shortening protein production development timelines", BIOTECHNOL. BIOENG, (2011), vol. 108, doi:doi:10.1002/bit.23103, pages 1570 - 1578, XP055076263

DOI:   http://dx.doi.org/10.1002/bit.23103
    - MADIN, K. ET AL., "A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: Plants apparently contain a suicide system directed at ribosomes", PROC. NATL. ACAD. SCI. U.S.A., (2000), vol. 97, doi:doi:10.1073/pnas.97.2.559, pages 559 - 564, XP002948543

DOI:   http://dx.doi.org/10.1073/pnas.97.2.559
    - TAKAI, K ET AL., "Practical cell-free protein synthesis system using purified wheat embryos", NAT. PROTOC., (2010), vol. 5, pages 227 - 238
    - CARLSON, E. D. ET AL., "Cell-free protein synthesis: Applications come of age", BIOTECHNOL. ADV., (2012), vol. 30, doi:doi:10.1016/j.biotechadv.2011.09.016, pages 1185 - 1194, XP055072004

DOI:   http://dx.doi.org/10.1016/j.biotechadv.2011.09.016
    - ZUBAY, G., ANN. REV. GENET, (1973), vol. 7, page 267
    - GOSHIMA, N. ET AL., "Human protein factory for converting the transcriptome into an in vitro-expressed proteome", NAT. METHODS, (2008), vol. 5, pages 1011 - 1017
    - HOFFMANN, M. ET AL., Biotechnol Annu Rev, ELSEVIER, (2004), vol. 10, pages 1 - 30
    - JACKSON, R. J. ET AL., Methods Enzymol, ACADEMIC PRESS, (1983), vol. 96, pages 50 - 74
    - EZURE, T ET AL., "A cell- free protein synthesis system from insect cells", METHODS MOL. BIOL., (2010), vol. 607, pages 31 - 42, XP009164449
    - KUBICK, S ET AL., Current Topics in Membranes, ACADEMIC PRESS, (2009), vol. 63, pages 25 - 49
    - TARUI, H. ET AL., "Establishment and characterization of cell-free translation/glycosylation in insect cell (Spodoptera frugiperda 21) extract prepared with high pressure treatment", APPL. MICROBIOL. BIOTECHNOL., (2001), vol. 55, doi:doi:10.1007/s002530000534, pages 446 - 453, XP002272560

DOI:   http://dx.doi.org/10.1007/s002530000534
    - KOVTUN, 0. ET AL., "Towards the construction of expressed proteomes using a Leishmania tarentolae based cell-free expression system", PLOS ONE, (2010), vol. 5, page EL4388
    - MUREEV, S. ET AL., "Species-independent translational leaders facilitate cell-free expression", NAT. BIOTECHNOL, (2009), vol. 27, pages 747 - 752
    - "Cell-Free Protein Production", MIKAMI, S. ET AL., Methods in Molecular Biology, HUMANA PRESS, (2010), vol. 607, pages 43 - 52
    - BRADFORD MM., "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.", ANAL BIOCHEM, (1976), vol. 72, doi:doi:10.1016/0003-2697(76)90527-3, pages 248 - 54, XP025650297

DOI:   http://dx.doi.org/10.1016/0003-2697(76)90527-3
    - BUNTRU M; VOGEL S; SPIEGEL H; SCHILLBERG S., "Tobacco BY-2 cell-free lysate: an alternative and highly-productive plant-based in vitro translation system.", BMC BIOTECHNOL., (2014), vol. 14, doi:doi:10.1186/1472-6750-14-37, page 37, XP021184658

DOI:   http://dx.doi.org/10.1186/1472-6750-14-37
    - BUNTRU M; VOGEL S; STOFF K; SPIEGEL H; SCHILLBERG S., "A Versatile Coupled Cell-Free Transcription-Translation System Based on Tobacco BY-2 Cell Lysate", BIOTECHNOL. BIOENG., (2015), vol. 112, no. 5, doi:doi:10.1002/BIT.25502, pages 867 - 78, XP002768481

DOI:   http://dx.doi.org/10.1002/BIT.25502
    - GURSINSKY T; SCHULZ B; BEHRENS SE., "Replication of Tomato bushy stunt virus RNA in a plant in vitro system", VIROLOGY, (2009), vol. 390, no. 2, doi:doi:10.1016/j.virol.2009.05.009, pages 250 - 60, XP026284545

DOI:   http://dx.doi.org/10.1016/j.virol.2009.05.009
    - HODGMAN CE; JEWETT MC, "Optimized extract preparation methods and reaction conditions for improved yeast cell-free protein synthesis", BIOTECHNOL BIOENG, (2013), vol. 110, no. 10, pages 2643 - 54
    - KOMODA K; NAITO S; ISHIKAWA M., "Replication of plant RNA virus genomes in a cell-free extract of evacuolated plant protoplasts.", PROC NATL ACAD SCI USA, (2004), vol. 101, no. 7, pages 1863 - 1867
The EPO accepts no responsibility for the accuracy of data originating from other authorities; in particular, it does not guarantee that it is complete, up to date or fit for specific purposes.