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Extract from the Register of European Patents

EP About this file: EP3450570

EP3450570 - METHOD FOR EVALUATING, IN VIVO, ACTIVITY OF RNA-GUIDED NUCLEASE IN HIGH-THROUGHPUT MANNER [Right-click to bookmark this link]
StatusNo opposition filed within time limit
Status updated on  14.01.2022
Database last updated on 02.11.2024
FormerThe patent has been granted
Status updated on  05.02.2021
FormerGrant of patent is intended
Status updated on  28.09.2020
FormerRequest for examination was made
Status updated on  01.02.2019
FormerThe international publication has been made
Status updated on  04.11.2017
Most recent event   Tooltip13.09.2024Lapse of the patent in a contracting state
New state(s): MT		published on 16.10.2024  [2024/42]
Applicant(s)For all designated states
Industry-Academic Cooperation Foundation, Yonsei University
50, Yonsei-ro
Seodaemun-gu
Seoul 03722 / KR
[2019/49]
Former [2019/10]For all designated states
Industry-Academic Cooperation Foundation Yonsei University
50 Yonsei-ro
Seodaemun-gu
Seoul 03772 / KR
Inventor(s)01 / KIM, Hyong Bum
106-703 280 Wangsimni-ro
Seongdong-gu
Seoul 04751 / KR
02 / KIM, Hui Kwon
64-6 Eunjin-ro
Munbaek-myeon
Jincheon-gun Chungcheongbuk-do 27867 / KR
03 / SONG, Myung Jae
203-401 111 Dobong-ro 136-gil
Dobong-gu
Seoul 01409 / KR
 [2019/10]
Representative(s)dompatent von Kreisler Selting Werner - Partnerschaft von Patent- und Rechtsanwälten mbB
Deichmannhaus am Dom
Bahnhofsvorplatz 1
50667 Köln / DE
[2019/10]
Application number, filing date17789981.228.04.2017
[2019/10]
WO2017KR04610
Priority number, dateKR2016005236528.04.2016         Original published format: KR 20160052365
[2019/10]
Filing languageKO
Procedural languageEN
PublicationType: A1 Application with search report
No.:WO2017188797
Date:02.11.2017
Language:KO
[2017/44]
Type: A1 Application with search report 
No.:EP3450570
Date:06.03.2019
Language:EN
[2019/10]
Type: B1 Patent specification 
No.:EP3450570
Date:10.03.2021
Language:EN
[2021/10]
Search report(s)International search report - published on:KR02.11.2017
(Supplementary) European search report - dispatched on:EP21.10.2019
ClassificationIPC:C12Q1/42, C12N15/63, C12N9/22, C12N15/10, C40B40/06, C12N15/11, G16B30/00
[2019/47]
CPC:
C12N9/22 (EP,KR,US); C12Q1/68 (KR); C12N15/1093 (EP,US);
C12N15/63 (KR,US); C12Q1/42 (EP,KR,US); C12Y301/00 (EP,KR,US);
C40B40/06 (US); C40B40/08 (KR,US); C40B50/06 (KR,US);
G16B30/00 (EP,US); G16B30/20 (EP,KR,US); C12N15/111 (EP,US);
C12N2310/20 (EP,US); C12N2320/11 (EP,US); C12N2330/31 (EP,US);
Y02A50/30 (US) (-)
Former IPC [2019/10]C12Q1/68, C12Q1/42, C12N15/63, C12N9/22, G06F19/22
Designated contracting statesAL,   AT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   LV,   MC,   MK,   MT,   NL,   NO,   PL,   PT,   RO,   RS,   SE,   SI,   SK,   SM,   TR [2019/10]
Extension statesBANot yet paid
MENot yet paid
Validation statesMANot yet paid
MDNot yet paid
TitleGerman:VERFAHREN ZUR IN-VIVO-BEURTEILUNG DER AKTIVITÄT VON RNA-GESTEUERTER NUKLEASE AUF HOCHDURCHSATZWEISE[2019/10]
English:METHOD FOR EVALUATING, IN VIVO, ACTIVITY OF RNA-GUIDED NUCLEASE IN HIGH-THROUGHPUT MANNER[2019/10]
French:PROCÉDÉ D'ÉVALUATION, IN VIVO, DE L'ACTIVITÉ D'UNE NUCLÉASE GUIDÉE PAR ARN DE MANIÈRE TRÈS EFFICACE[2019/10]
Entry into regional phase28.11.2018Translation filed 
28.11.2018National basic fee paid 
28.11.2018Search fee paid 
28.11.2018Designation fee(s) paid 
28.11.2018Examination fee paid 
Examination proceduredeletedDate on which the examining division has become responsible
28.11.2018Examination requested  [2019/10]
18.05.2020Amendment by applicant (claims and/or description)
28.09.2020Communication of intention to grant the patent
02.02.2021Fee for grant paid
02.02.2021Fee for publishing/printing paid
02.02.2021Receipt of the translation of the claim(s)
Opposition(s)13.12.2021No opposition filed within time limit [2022/07]
Fees paidRenewal fee
26.04.2019Renewal fee patent year 03
25.03.2020Renewal fee patent year 04
Opt-out from the exclusive  Tooltip
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Patent Court		 See the Register of the Unified Patent Court for opt-out data
Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Lapses during opposition  TooltipHU28.04.2017
AL10.03.2021
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BG10.06.2021
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GR11.06.2021
IS10.07.2021
PT12.07.2021
[2024/42]
Former [2024/29]HU28.04.2017
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Documents cited:Search[Y]WO2014099744  (HARVARD COLLEGE [US]) [Y] 1-15 * the whole document * * Examples X, XIII, Fig. 13A-1 *;
 [Y]  - PATRICK D HSU ET AL, "DNA targeting specificity of RNA-guided Cas9 nucleases", HHS PUBLIC ACCESS AUTHOR MANUSCRIPT, (20130721), vol. 31, no. 9, doi:10.1038/nbt.2647, pages 1 - 17, XP055513966 [Y] 1-15 * the whole document * * p. 2, third para.; Fig. 1-2 *

DOI:   http://dx.doi.org/10.1038/nbt.2647
 [T]  - Soobin Jung, "Genome-wide prediction and profiling of Off-target cleavage by CRISPR-Cas9", (20180601), pages 1 - 57, URL: https://ir.ymlib.yonsei.ac.kr/bitstream/22282913/166400/1/T014876.pdf, (20191010), XP055630777 [T] 1-15 * the whole document * * p. 19-21 *
 [T]  - DAQI WANG ET AL, "Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning", NATURE COMMUNICATIONS, (20190919), vol. 10, no. 1, doi:10.1038/s41467-019-12281-8, XP055631062 [T] 1-15 * the whole document * * Fig. 2 *

DOI:   http://dx.doi.org/10.1038/s41467-019-12281-8
International search[X]EP3009511  (BROAD INST INC [US], et al) [X] 24-28 * See paragraphs [0353] and [0425]; claims 1 and 8-10. *;
 [XA]  - AGROTIS et al., "A New Age in Functional Genomics Using CRISPR/Cas9 in Arrayed Library Screening", Frontiers in Genetics, (20150900), vol. 6, no. 300, pages 1 - 15, XP055434268 [X] 15-23 * See abstract; pages 5 and 7; figure 1; table 3. * [A] 1-14, 24-28

DOI:   http://dx.doi.org/10.3389/fgene.2015.00300
 [X]  - YAMANO et al., "Crystal Structure of Cpfl in Complex with Guide RNA and Target DNA", Cell, (20160421), vol. 1, no. 65, pages 949 - 962, XP029530759 [X] 20 * See abstract; figure 2(A). *

DOI:   http://dx.doi.org/10.1016/j.cell.2016.04.003
 [X]  - NISHIMASU et al., "Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA", Cell, (20140227), vol. 156, pages 935 - 949, XP055423859 [X] 20 * See abstract; figure 4(A). *

DOI:   http://dx.doi.org/10.1016/j.cell.2014.02.001
 [A]  - PENG et al., "High-throughput Screens in Mammalian Cells Using the CRISPR-Cas9 System", The FEBS Journal, (20150316), vol. 282, pages 2089 - 2096, XP055434292 [A] 1-28 * See abstract; page 2091; figures 1 and 2. *

DOI:   http://dx.doi.org/10.1111/febs.13251
 [A]  - O'GEEN et al., "A Genome-wide Analysis of Cas9 Binding Specificity Using ChIP-seq and Targeted Sequence Capture", Nucleic Acids Research, (20150000), vol. 43, no. 6, pages 3389 - 3404, XP055434273 [A] 1-28 * See abstract; pages 3390-3392; figure 7. *

DOI:   http://dx.doi.org/10.1093/nar/gkv137
 [A]  - VIDIGAL et al., "Rapid and Efficient One-step Generation of Paired gRNA CRISPR-Cas9 Libraries", Nature Communications, (20150000), vol. 6, no. 8083, pages 1 - 7, XP055340818 [A] 1-28 * See abstract; page 2; figures 1, 2 and 4. *

DOI:   http://dx.doi.org/10.1038/ncomms9083
 [PX]  - KIM et al., "In vivo High-throughput Profiling of CRISPR-Cpfl Activity", Nature Methods, (20170200), vol. 14, no. 2, pages 153 - 159, XP055434289 [PX] 1-23 * See abstract. *

DOI:   http://dx.doi.org/10.1038/nmeth.4104
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