blank Quick help
blank Maintenance news

Scheduled maintenance

Regular maintenance outages:
between 05.00 and 05.15 hrs CET (Monday to Sunday).

Other outages
Availability

2022.02.11

More...
blank News flashes

News Flashes

New version of the European Patent Register – SPC proceedings information in the Unitary Patent Register.

2024-07-24

More...
blank Related links

Release notes
European Patent Register Assistant
European Patent Register Resource Book
European Patent Register – pocket guide
FAQ - European Patent Register
Federated Register (Flyer)
Global Dossier (Flyer)
Register events
Deep link coverage
Federated Register content
The Patent Information Tour
Fair use charter

Extract from the Register of European Patents

EP About this file: EP3555284

EP3555284 - COMPOSITIONS AND METHODS USING CAS9 WITH ENHANCED SPACER ACQUISITION FUNCTION [Right-click to bookmark this link]
StatusNo opposition filed within time limit
Status updated on  07.10.2022
Database last updated on 03.10.2024
FormerThe patent has been granted
Status updated on  29.10.2021
FormerGrant of patent is intended
Status updated on  13.07.2021
FormerRequest for examination was made
Status updated on  20.09.2019
FormerThe international publication has been made
Status updated on  23.06.2018
Most recent event   Tooltip13.09.2024Lapse of the patent in a contracting state
New state(s): MT		published on 16.10.2024 [2024/42]
Applicant(s)For all designated states
The Rockefeller University
1230 York Avenue
New York, NY 10021 / US
[2019/43]
Inventor(s)01 / MARRAFFINI, Luciano
501 Hicks Street 201
Brooklyn, NY 11231 / US
02 / HELLER, Robert
1230 York Avenue
Box 120
New York, NY 10065 / US
03 / BIKARD, David
10 Rue Joseph Liouville
75015 Paris / FR
 [2019/43]
Representative(s)FRKelly
Waterways House
Grand Canal Quay
Dublin D02 PD39 / IE
[N/P]
Former [2021/48]FRKelly
27 Clyde Road
Dublin D04 F838 / IE
Former [2019/43]FRKelly
27 Clyde Road
Dublin D04 F838 / IE
Application number, filing date17880660.018.12.2017
[2019/43]
WO2017US66937
Priority number, dateUS201662435406P16.12.2016         Original published format: US 201662435406 P
[2019/43]
Filing languageEN
Procedural languageEN
PublicationType: A1 Application with search report
No.:WO2018112451
Date:21.06.2018
Language:EN
[2018/25]
Type: A1 Application with search report 
No.:EP3555284
Date:23.10.2019
Language:EN
The application published by WIPO in one of the EPO official languages on 21.06.2018 takes the place of the publication of the European patent application.
[2019/43]
Type: B1 Patent specification 
No.:EP3555284
Date:01.12.2021
Language:EN
[2021/48]
Search report(s)International search report - published on:US21.06.2018
(Supplementary) European search report - dispatched on:EP26.06.2020
ClassificationIPC:C12N15/00, C12N15/74, C12P21/06, C12C11/00, C12G1/00, C07H21/04
[2019/43]
CPC:
C12N9/22 (EP,US); A23C9/123 (US); C12C11/00 (EP);
C12G1/00 (EP,US); C12N15/74 (US); C12N2310/20 (US);
C12N2795/00011 (US); C12N2820/55 (US) (-)
Designated contracting statesAL,   AT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   LV,   MC,   MK,   MT,   NL,   NO,   PL,   PT,   RO,   RS,   SE,   SI,   SK,   SM,   TR [2019/43]
Extension statesBANot yet paid
MENot yet paid
Validation statesMANot yet paid
MDNot yet paid
TNNot yet paid
TitleGerman:ZUSAMMENSETZUNGEN UND VERFAHREN UNTER VERWENDUNG VON CAS9 MIT VERBESSERTER ABSTANDSERFASSUNGSFUNKTION[2019/43]
English:COMPOSITIONS AND METHODS USING CAS9 WITH ENHANCED SPACER ACQUISITION FUNCTION[2019/43]
French:COMPOSITIONS ET PROCÉDÉS UTILISANT CAS9 AVEC UNE FONCTION D'ACQUISITION D'ESPACEUR AMÉLIORÉE[2019/43]
Entry into regional phase09.07.2019National basic fee paid 
09.07.2019Search fee paid 
09.07.2019Designation fee(s) paid 
09.07.2019Examination fee paid 
Examination procedure09.07.2019Examination requested  [2019/43]
14.01.2021Amendment by applicant (claims and/or description)
14.07.2021Communication of intention to grant the patent
22.10.2021Fee for grant paid
22.10.2021Fee for publishing/printing paid
22.10.2021Receipt of the translation of the claim(s)
Opposition(s)02.09.2022No opposition filed within time limit [2022/45]
Fees paidRenewal fee
27.11.2019Renewal fee patent year 03
22.12.2020Renewal fee patent year 04
Opt-out from the exclusive  Tooltip
competence of the Unified
Patent Court		 See the Register of the Unified Patent Court for opt-out data
Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Lapses during opposition  TooltipHU18.12.2017
AL01.12.2021
AT01.12.2021
CY01.12.2021
CZ01.12.2021
DK01.12.2021
EE01.12.2021
ES01.12.2021
FI01.12.2021
HR01.12.2021
IT01.12.2021
LT01.12.2021
LV01.12.2021
MC01.12.2021
MK01.12.2021
MT01.12.2021
NL01.12.2021
PL01.12.2021
RO01.12.2021
RS01.12.2021
SE01.12.2021
SI01.12.2021
SK01.12.2021
SM01.12.2021
IE18.12.2021
LU18.12.2021
BE31.12.2021
BG01.03.2022
NO01.03.2022
GR02.03.2022
IS01.04.2022
PT01.04.2022
[2024/42]
Former [2024/23]HU18.12.2017
AL01.12.2021
AT01.12.2021
CY01.12.2021
CZ01.12.2021
DK01.12.2021
EE01.12.2021
ES01.12.2021
FI01.12.2021
HR01.12.2021
IT01.12.2021
LT01.12.2021
LV01.12.2021
MC01.12.2021
MK01.12.2021
NL01.12.2021
PL01.12.2021
RO01.12.2021
RS01.12.2021
SE01.12.2021
SI01.12.2021
SK01.12.2021
SM01.12.2021
IE18.12.2021
LU18.12.2021
BE31.12.2021
BG01.03.2022
NO01.03.2022
GR02.03.2022
IS01.04.2022
PT01.04.2022
Former [2023/33]HU18.12.2017
AL01.12.2021
AT01.12.2021
CY01.12.2021
CZ01.12.2021
DK01.12.2021
EE01.12.2021
ES01.12.2021
FI01.12.2021
HR01.12.2021
IT01.12.2021
LT01.12.2021
LV01.12.2021
MC01.12.2021
NL01.12.2021
PL01.12.2021
RO01.12.2021
RS01.12.2021
SE01.12.2021
SI01.12.2021
SK01.12.2021
SM01.12.2021
IE18.12.2021
LU18.12.2021
BE31.12.2021
BG01.03.2022
NO01.03.2022
GR02.03.2022
IS01.04.2022
PT01.04.2022
Former [2023/30]AL01.12.2021
AT01.12.2021
CY01.12.2021
CZ01.12.2021
DK01.12.2021
EE01.12.2021
ES01.12.2021
FI01.12.2021
HR01.12.2021
IT01.12.2021
LT01.12.2021
LV01.12.2021
MC01.12.2021
NL01.12.2021
PL01.12.2021
RO01.12.2021
RS01.12.2021
SE01.12.2021
SI01.12.2021
SK01.12.2021
SM01.12.2021
IE18.12.2021
LU18.12.2021
BE31.12.2021
BG01.03.2022
NO01.03.2022
GR02.03.2022
IS01.04.2022
PT01.04.2022
Former [2023/27]AL01.12.2021
AT01.12.2021
CZ01.12.2021
DK01.12.2021
EE01.12.2021
ES01.12.2021
FI01.12.2021
HR01.12.2021
IT01.12.2021
LT01.12.2021
LV01.12.2021
MC01.12.2021
NL01.12.2021
PL01.12.2021
RO01.12.2021
RS01.12.2021
SE01.12.2021
SI01.12.2021
SK01.12.2021
SM01.12.2021
IE18.12.2021
LU18.12.2021
BE31.12.2021
BG01.03.2022
NO01.03.2022
GR02.03.2022
IS01.04.2022
PT01.04.2022
Former [2023/01]AL01.12.2021
AT01.12.2021
CZ01.12.2021
DK01.12.2021
EE01.12.2021
ES01.12.2021
FI01.12.2021
HR01.12.2021
LT01.12.2021
LV01.12.2021
MC01.12.2021
NL01.12.2021
PL01.12.2021
RO01.12.2021
RS01.12.2021
SE01.12.2021
SI01.12.2021
SK01.12.2021
SM01.12.2021
IE18.12.2021
LU18.12.2021
BE31.12.2021
BG01.03.2022
NO01.03.2022
GR02.03.2022
IS01.04.2022
PT01.04.2022
Former [2022/49]AL01.12.2021
AT01.12.2021
CZ01.12.2021
DK01.12.2021
EE01.12.2021
ES01.12.2021
FI01.12.2021
HR01.12.2021
LT01.12.2021
LV01.12.2021
MC01.12.2021
NL01.12.2021
PL01.12.2021
RO01.12.2021
RS01.12.2021
SE01.12.2021
SK01.12.2021
SM01.12.2021
IE18.12.2021
LU18.12.2021
BG01.03.2022
NO01.03.2022
GR02.03.2022
IS01.04.2022
PT01.04.2022
Former [2022/48]AT01.12.2021
CZ01.12.2021
DK01.12.2021
EE01.12.2021
ES01.12.2021
FI01.12.2021
HR01.12.2021
LT01.12.2021
LV01.12.2021
MC01.12.2021
NL01.12.2021
PL01.12.2021
RO01.12.2021
RS01.12.2021
SE01.12.2021
SK01.12.2021
SM01.12.2021
IE18.12.2021
LU18.12.2021
BG01.03.2022
NO01.03.2022
GR02.03.2022
IS01.04.2022
PT01.04.2022
Former [2022/46]AT01.12.2021
CZ01.12.2021
DK01.12.2021
EE01.12.2021
ES01.12.2021
FI01.12.2021
HR01.12.2021
LT01.12.2021
LV01.12.2021
MC01.12.2021
NL01.12.2021
PL01.12.2021
RO01.12.2021
RS01.12.2021
SE01.12.2021
SK01.12.2021
SM01.12.2021
LU18.12.2021
BG01.03.2022
NO01.03.2022
GR02.03.2022
IS01.04.2022
PT01.04.2022
Former [2022/42]AT01.12.2021
CZ01.12.2021
EE01.12.2021
ES01.12.2021
FI01.12.2021
HR01.12.2021
LT01.12.2021
LV01.12.2021
MC01.12.2021
NL01.12.2021
PL01.12.2021
RO01.12.2021
RS01.12.2021
SE01.12.2021
SK01.12.2021
SM01.12.2021
BG01.03.2022
NO01.03.2022
GR02.03.2022
IS01.04.2022
PT01.04.2022
Former [2022/39]AT01.12.2021
CZ01.12.2021
EE01.12.2021
ES01.12.2021
FI01.12.2021
HR01.12.2021
LT01.12.2021
LV01.12.2021
MC01.12.2021
NL01.12.2021
PL01.12.2021
RO01.12.2021
RS01.12.2021
SE01.12.2021
SK01.12.2021
SM01.12.2021
BG01.03.2022
NO01.03.2022
GR02.03.2022
PT01.04.2022
Former [2022/35]AT01.12.2021
CZ01.12.2021
EE01.12.2021
ES01.12.2021
FI01.12.2021
HR01.12.2021
LT01.12.2021
LV01.12.2021
NL01.12.2021
PL01.12.2021
RO01.12.2021
RS01.12.2021
SE01.12.2021
SK01.12.2021
SM01.12.2021
BG01.03.2022
NO01.03.2022
GR02.03.2022
PT01.04.2022
Former [2022/34]AT01.12.2021
EE01.12.2021
ES01.12.2021
FI01.12.2021
HR01.12.2021
LT01.12.2021
LV01.12.2021
NL01.12.2021
PL01.12.2021
RO01.12.2021
RS01.12.2021
SE01.12.2021
SM01.12.2021
BG01.03.2022
NO01.03.2022
GR02.03.2022
PT01.04.2022
Former [2022/33]AT01.12.2021
ES01.12.2021
FI01.12.2021
HR01.12.2021
LT01.12.2021
LV01.12.2021
NL01.12.2021
PL01.12.2021
RS01.12.2021
SE01.12.2021
SM01.12.2021
BG01.03.2022
NO01.03.2022
GR02.03.2022
Former [2022/30]AT01.12.2021
ES01.12.2021
FI01.12.2021
HR01.12.2021
LT01.12.2021
LV01.12.2021
NL01.12.2021
PL01.12.2021
RS01.12.2021
SE01.12.2021
BG01.03.2022
NO01.03.2022
GR02.03.2022
Former [2022/25]AT01.12.2021
ES01.12.2021
FI01.12.2021
HR01.12.2021
LT01.12.2021
LV01.12.2021
PL01.12.2021
RS01.12.2021
SE01.12.2021
BG01.03.2022
NO01.03.2022
GR02.03.2022
Former [2022/24]AT01.12.2021
ES01.12.2021
FI01.12.2021
HR01.12.2021
LT01.12.2021
LV01.12.2021
PL01.12.2021
RS01.12.2021
SE01.12.2021
BG01.03.2022
GR02.03.2022
Former [2022/23]AT01.12.2021
ES01.12.2021
FI01.12.2021
HR01.12.2021
LT01.12.2021
LV01.12.2021
PL01.12.2021
RS01.12.2021
BG01.03.2022
Former [2022/22]AT01.12.2021
FI01.12.2021
LT01.12.2021
RS01.12.2021
BG01.03.2022
Former [2022/21]AT01.12.2021
RS01.12.2021
BG01.03.2022
Former [2022/19]BG01.03.2022
Documents cited:Search[E]WO2018149888  (UNIV DEGLI STUDI DI TRENTO [IT]) [E] 1-5 * page 4, lines 15, 26 ** claims 4, 6, 8, 10 *;
 [AD]  - YUNZHOU WEI ET AL, "Cas9 function and host genome sampling in Type II-A CRISPR-Cas adaptation", GENES AND DEVELOPMENT., US, (20150215), vol. 29, no. 4, doi:10.1101/gad.257550.114, ISSN 0890-9369, pages 356 - 361, XP055703542 [AD] 1-15 * page 358, column l, paragraph 2 - page 360, column l, paragraph 1 *

DOI:   http://dx.doi.org/10.1101/gad.257550.114
 [AD]  - ROBERT HELER ET AL, "Cas9 specifies functional viral targets during CRISPR-Cas adaptation", NATURE, London, (20150301), vol. 519, no. 7542, doi:10.1038/nature14245, ISSN 0028-0836, pages 199 - 202, XP055703544 [AD] 1-15 * the whole document *

DOI:   http://dx.doi.org/10.1038/nature14245
 [AD]  - CAROLIN ANDERS ET AL, "Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease", NATURE, London, (20140101), vol. 513, no. 7519, doi:10.1038/nature13579, ISSN 0028-0836, pages 569 - 573, XP055240929 [AD] 1-15 * figure 2 *

DOI:   http://dx.doi.org/10.1038/nature13579
 [AD]  - M. JINEK ET AL, "Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation", SCIENCE, (20140314), vol. 343, no. 6176, doi:10.1126/science.1247997, ISSN 0036-8075, pages 1247997 - 1247997, XP055149157 [AD] 1-15 * figure 1 *

DOI:   http://dx.doi.org/10.1126/science.1247997
International search[Y]US2016319261  (JOUNG J KEITH [US], et al) [Y] 1-7 * , para [0003]-[0007], [0009], [0010], [0023], [0025], [0028], [0034], [0059], [0062], [0089], [0129], [0132], [0166], and SEQ ID NO: 1 *;
 [Y]  - NISHIMASU et al., "Crystal structure of Cas9 in complex with guide RNA and target DNA", Cell, (20140227), vol. 156, no. 5, pages 935 - 949, XP055423859 [Y] 1-7 * . Abstract; pg 936, Fig 1; pg 937, col 1, last para , and col 2, para 2 and last para; pg 938, col 1, col 2, up para, and Fig 2B; and pg 943, col 2, para 1, and Fig 5 *

DOI:   http://dx.doi.org/10.1016/j.cell.2014.02.001
 [Y]  - STEFFENS et al., "Efficient Site-Directed Saturation Mutagenesis Using Degenerate Oligonucleotides", J Biomol Tech, (20070701), vol. 18, no. 3, pages 147 - 9, XP055494185 [Y] 1-7 * . Abstract; and pg 147, col 1, para 1, and col 2, up para *
 [A]  - ESVELT et al., "Orthogonal Cas9 proteins for RNA-guided gene regulation and editing", Nat Methods, vol. 10, no. 11, (20130929), pages 1116 - 21, URL: doi:10.1038/nmeth.2681, XP055128928 [A] 1-7 * . PDF File: pg 1-19. Entire documentation, especially pg 2, para 3, and last para; pg 17, Fig 5; and pg 19, Table 1 *

DOI:   http://dx.doi.org/10.1038/nmeth.2681
 [A]  - LIA-BALDINI et al., "A molecular approach to dominance in hypophosphatasia", Hum Genet, vol. 109, no. 1, (20010703), pages 99 - 108, URL: DOI10.1007/s004390100546, XP055494188 [A] 1-7 * . Abstract *

DOI:   http://dx.doi.org/10.1007/s004390100546
 [A]  - ZHANG et al., "Comparison of non-canonical PAMs for CRISPR/Cas9-mediated DNA cleavage in human cells", Sci Kep, vol. 4, (20140423), URL: https://doi.org/10.1038/srep05405, XP055494195 [A] 1-7 * . PDF File: pg 1-5. Entire documentation especially Abstract; and pg 1, para 1 and para 2 *
 [PX]  - HELER et al., "Mutations in Cas9 enhance the rate of acquisition of viral spacer sequences during the CRISPR-Cas immune response", Mol Cell, vol. 65, no. 1, (20170105), pages 168 - 175, URL: doi:10.1016/j.molcel.2016.11.031, XP029876898 [PX] 1-7 * . Epub 201G Dec 22. PDF File: pg 1-17. Entire documentation, especially Abstract; pg 2, last para; pg 3; pg 4, up para; pg 7, up para; pg 9, para 3; pg 13, Fig 1; and pg 16, Fig 3 *

DOI:   http://dx.doi.org/10.1016/j.molcel.2016.11.031
The EPO accepts no responsibility for the accuracy of data originating from other authorities; in particular, it does not guarantee that it is complete, up to date or fit for specific purposes.