blank Quick help
blank Maintenance news

Scheduled maintenance

Regular maintenance outages:
between 05.00 and 05.15 hrs CET (Monday to Sunday).

Other outages
Availability

2022.02.11

More...
blank News flashes

Italy joins the Federated Register

Click here for more information on the Federated Register.

2025-06-12

More...
blank Related links

Release notes
European Patent Register Assistant
European Patent Register User Manual
European Patent Register – pocket guide
FAQ - European Patent Register
Federated Register (Flyer)
Global Dossier (Flyer)
Register events
Deep link coverage
Federated Register content
The Patent Information Tour
Fair use charter

Extract from the Register of European Patents

EP About this file: EP3521454

EP3521454 - DETECTION OF DNA THAT ORIGINATES FROM A SPECIFIC CELL-TYPE AND RELATED METHODS [Right-click to bookmark this link]
StatusThe application is deemed to be withdrawn
Status updated on  12.03.2021
Database last updated on 28.03.2026
FormerExamination is in progress
Status updated on  01.05.2020
FormerRequest for examination was made
Status updated on  14.02.2020
FormerThe application has been published
Status updated on  05.07.2019
Most recent event   Tooltip12.03.2021Application deemed to be withdrawnpublished on 14.04.2021  [2021/15]
Applicant(s)For all designated states
Eurofins LifeCodexx GmbH
Line-Eid-Strasse 3
78467 Konstanz / DE
[2020/15]
Former [2019/32]For all designated states
LifeCodexx AG
Line-Eid-Strasse 3
78467 Konstanz / DE
Inventor(s)01 / GRÖMMINGER, Sebastian
c/o Eurofins LifeCodexx GmbH
Line-Eid-Str. 3
78467 Konstanz / DE
02 / HOFMANN, Wera
c/o Eurofins LifeCodexx GmbH
Line-Eid-Str. 3
78467 Konstanz / DE
03 / SAID, Hamed
c/o Eurofins LifeCodexx GmbH
Line-Eid-Str. 3
78467 Konstanz / DE
04 / SACHSE, Matthias
c/o Eurofins LifeCodexx GmbH
Line-Eid-Str. 3
78467 Konstanz / DE
 [2020/13]
Former [2019/32]01 / GRÖMMINGER, Sebastian
c/o LifeCodexx AG
Line-Eid-Str. 3
78467 Konstanz / DE
02 / HOFMANN, Wera
c/o LifeCodexx AG
Line-Eid-Str. 3
78467 Konstanz / DE
03 / SAID, Hamed
c/o LifeCodexx AG
Line-Eid-Str. 3
78467 Konstanz / DE
04 / SACHSE, Matthias
c/o LifeCodexx AG
Line-Eid-Str. 3
78467 Konstanz / DE
Representative(s)Dörries, Hans Ulrich
df-mp Patentanwälte Rechtsanwälte PartG mbB
Theatinerstraße 16
80333 München / DE
[N/P]
Former [2019/32]Dörries, Hans Ulrich
df-mp Dörries Frank-Molnia & Pohlman
Patentanwälte Rechtsanwälte PartG mbB
Theatinerstrasse 16
80333 München / DE
Application number, filing date18214042.608.05.2015
[2019/32]
Priority number, dateEP2014016776909.05.2014         Original published format: EP 14167769
EP2014016777509.05.2014         Original published format: EP 14167775
[2019/32]
Filing languageEN
Procedural languageEN
PublicationType: A1 Application with search report 
No.:EP3521454
Date:07.08.2019
Language:EN
[2019/32]
Search report(s)(Supplementary) European search report - dispatched on:EP02.05.2019
ClassificationIPC:C12Q1/6827, C12Q1/6886, C12Q1/6883
[2019/32]
CPC:
C12Q1/6883 (EP,CN,US); C12Q1/6827 (EP,CN,US); C12Q1/6886 (EP,CN,US);
G16B30/00 (US); C12Q2600/154 (EP,CN,US)
C-Set:
C12Q1/6827, C12Q2523/125 (CN,EP,US)
Designated contracting statesAL,   AT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   LV,   MC,   MK,   MT,   NL,   NO,   PL,   PT,   RO,   RS,   SE,   SI,   SK,   SM,   TR [2020/12]
Former [2019/32]AL,  AT,  BE,  BG,  CH,  CY,  CZ,  DE,  DK,  EE,  ES,  FI,  FR,  GB,  GR,  HR,  HU,  IE,  IS,  IT,  LI,  LT,  LU,  LV,  MC,  MK,  MT,  NL,  NO,  PL,  PT,  RO,  RS,  SE,  SI,  SK,  SM,  TR 
Extension statesBANot yet paid
MENot yet paid
Validation statesMANot yet paid
TitleGerman:NACHWEIS VON AUS EINEM SPEZIFISCHEN ZELLTYP STAMMENDER DNA UND ZUGEHÖRIGE VERFAHREN[2019/32]
English:DETECTION OF DNA THAT ORIGINATES FROM A SPECIFIC CELL-TYPE AND RELATED METHODS[2019/32]
French:DÉTECTION D'ADN PROVENANT D'UN TYPE CELLULAIRE SPÉCIFIQUE ET PROCÉDÉS ASSOCIÉS[2019/32]
Examination procedure07.02.2020Amendment by applicant (claims and/or description)
07.02.2020Examination requested  [2020/12]
07.02.2020Date on which the examining division has become responsible
07.05.2020Despatch of a communication from the examining division (Time limit: M06)
18.11.2020Application deemed to be withdrawn, date of legal effect  [2021/15]
08.12.2020Despatch of communication that the application is deemed to be withdrawn, reason: reply to the communication from the examining division not received in time  [2021/15]
Parent application(s)   TooltipEP15723189.5  / EP3140421
Fees paidRenewal fee
16.04.2019Renewal fee patent year 03
16.04.2019Renewal fee patent year 04
16.04.2019Renewal fee patent year 05
19.05.2020Renewal fee patent year 06
Opt-out from the exclusive  Tooltip
competence of the Unified
Patent Court		 See the Register of the Unified Patent Court for opt-out data
Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Documents cited:Search[XYI] WO2008128198  (USC STEVENS UNIVERSITY OF SOUT et al.)
 [YD] WO2010033639  (SEQUENOM INC et al.)
 [YD] WO2011034631  (SEQUENOM INC et al.)
 [YD] WO2011092592  (NIPD GENETICS LTD et al.)
 [YD] WO2005118852  (UNIV HONG KONG CHINESE et al.)
 [XYI]   WEISENBERGER DANIEL J ET AL: "DNA methylation analysis by digital bisulfite genomic sequencing and digital MethyLight", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, GB, vol. 36, no. 14, 1 August 2008 (2008-08-01), pages 4689 - 4698, XP002585338, ISSN: 1362-4962, [retrieved on 20080715], DOI: 10.1093/NAR/GKN455

DOI:   http://dx.doi.org/10.1093/NAR/GKN455
 [Y]   HE Q ET AL: "Development of a multiplex MethyLight assay for the detection of multigene methylation in human colorectal cancer", CANCER GENETICS AND CYTOGENETICS, ELSEVIER SCIENCE PUBLISHING, NEW YORK, NY, US, vol. 202, no. 1, 1 October 2010 (2010-10-01), pages 1 - 10, XP027246507, ISSN: 0165-4608, [retrieved on 20100827]
 [Y]   CHAN K C ALLEN ET AL: "Hypermethylated RASSF1A in maternal plasma: A universal fetal DNA marker that improves the reliability of noninvasive prenatal diagnosis", CLINICAL CHEMISTRY, AMERICAN ASSOCIATION FOR CLINICAL CHEMISTRY, WASHINGTON, DC, vol. 52, no. 12, 1 December 2006 (2006-12-01), pages 2211 - 2218, XP009089108, ISSN: 0009-9147, DOI: 10.1373/CLINCHEM.2006.074997

DOI:   http://dx.doi.org/10.1373/clinchem.2006.074997
 [Y]   CAMPAN MIHAELA ET AL: "MethyLight.", METHODS IN MOLECULAR BIOLOGY (CLIFTON, N.J.) 2009, vol. 507, 2009, pages 325 - 337, XP002730440, ISSN: 1064-3745

DOI:   http://dx.doi.org/10.1007/978-1-59745-522-0_23
 [Y]   WEISENBERGER D J ET AL: "Analysis of repetitive element DNA methylation by MethyLight", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, GB, vol. 33, no. 21, 1 January 2005 (2005-01-01), pages 6823 - 6836, XP002422120, ISSN: 0305-1048, DOI: 10.1093/NAR/GKI987

DOI:   http://dx.doi.org/10.1093/nar/gki987
 [Y]   EKATERINA OLKHOV-MITSEL ET AL: "Novel Multiplex MethyLight Protocol for Detection of DNA Methylation in Patient Tissues and Bodily Fluids", SCIENTIFIC REPORTS, vol. 4, 21 March 2014 (2014-03-21), XP055143311, DOI: 10.1038/srep04432

DOI:   http://dx.doi.org/10.1038/srep04432
 [Y]   SUZANNE SNELLENBERG ET AL: "Development of a multiplex methylation-specific PCR as candidate triage test for women with an HPV-positive cervical scrape", BMC CANCER, BIOMED CENTRAL, LONDON, GB, vol. 12, no. 1, 23 November 2012 (2012-11-23), pages 551, XP021128495, ISSN: 1471-2407, DOI: 10.1186/1471-2407-12-551

DOI:   http://dx.doi.org/10.1186/1471-2407-12-551
 [Y]   A. O. H. NYGREN ET AL: "Quantification of Fetal DNA by Use of Methylation-Based DNA Discrimination", CLINICAL CHEMISTRY, vol. 56, no. 10, 20 August 2010 (2010-08-20), pages 1627 - 1635, XP055089811, ISSN: 0009-9147, DOI: 10.1373/clinchem.2010.146290

DOI:   http://dx.doi.org/10.1373/clinchem.2010.146290
 [Y]   THERESA SWIFT-SCANLAN ET AL: "Two-color quantitative multiplex methylation-specific PCR", BIOTECHNIQUES, vol. 40, no. 2, 1 February 2006 (2006-02-01), pages 210 - 219, XP055143300, ISSN: 0736-6205, DOI: 10.2144/000112097

DOI:   http://dx.doi.org/10.2144/000112097
by applicantWO2010033639
 WO2011034631
 WO2012092592
 WO2007132166
 WO2007132167
 US5804375
 EP0543942
 US6258569
 EP0792374
 US6727356
 EP1235938
 US6929907
 EP0706649
 US5994056
 EP0512334
 WO2011092592
 EP1751307
 US6200756
 EP0954608
 US6331393
 EP1185695
   MANDEL; METAIS, CR ACAD SCI PARIS, vol. 142, 1948, pages 241
   SORENSON ET AL., CANCER EPIDEMIOL BIOMARKERS PREV, vol. 3, 1994, pages 67
   VASSIOUKHIN ET AL., BR J HAEMATOL, vol. 86, 1994, pages 774
   LO ET AL., LANCET, vol. 350, 1997, pages 485
   MULLER; WIDSCHWENDTER, EXPERT REV MOL DIAGN, vol. 3, 2003, pages 443
   LO ET AL., CLIN CHEM, vol. 46, 2000, pages 1301
   SMID ET AL., PRENAT DIAGN, vol. 26, 2006, pages 785
   LO ET AL., AM J HUM GENET, vol. 64, 1999, pages 218
   KAWAI ET AL., NUCLEIC ACIDS RES, vol. 21, 1993, pages 5604
   MASUZAKI ET AL., J MED GENET, vol. 41, 2004, pages 289
   FIORI ET AL., HUM REPROD, vol. 19, 2004, pages 723
   CHIM ET AL., PROC NATL ACAD SCI USA, vol. 102, 2005, pages 14753
   CHIU ET AL., AM J PATHOL, vol. 170, 2007, pages 941
   OLD ET AL., REPROD BIOMED ONLINE, vol. 15, 2007, pages 22
   CHIM ET AL., CLIN CHEM, vol. 54, 2008, pages 500
   NYGREN ET AL., CLIN CHEM, vol. 65, 2010, pages 10
   LO, N ENG J MED, vol. 339, 1998, pages 1734
   GO ET AL., HUMAN REPROD UPDATE, vol. 17, 2011, pages 372
   LO ET AL., AM J HUM GENET, vol. 62, 1998, pages 768
   LO ET AL., CLIN CHEM, vol. 45, 1999, pages 184
   YU ET AL., INT J MOL SCI, vol. 14, 2013, pages 7571
   HAHN ET AL., PLACENTA, vol. 32, 2011, pages S17
   LI ET AL., EPIGENETICS, vol. 6, 2011, pages 307
   HA ET AL., PLOS ONE, vol. 6, no. 10, 2011, pages e25969
   OUTINEN ET AL., PLOS ONE, vol. 7, no. 2, 2012, pages e31455
   FORSBLOM ET AL., PLOS ONE, vol. 9, no. 2, 2014, pages e87741
   CHAN ET AL., CLIN CHEM, vol. 50, 2004, pages 88
   KIMURA ET AL., NAGOYA J MED SCI, vol. 73, 2011, pages 129
   LO ET AL., SCI TRANSL MED, vol. 2, 2010, pages 61
   ELSHIMALI ET AL., INT J MOL SCI, vol. 14, 2013, pages 18925
   ZEERLEDER, CRIT CARE, vol. 10, 2006, pages 142
   KIRSCH ET AL., ANN NY ACAD SCI, vol. 1137, 2008, pages 135
   RUSSELL: "iGenetics", 2010, PEARSON BENJAMIN CUMMINGS, pages: 24 - 27
   GAUTHIER ET AL., J IMMUNOL, vol. 156, 1996, pages 1151
   LO ET AL., CANCER RES, vol. 59, 1999, pages 3899
   BIRCH ET AL., CLIN CHEM, vol. 51, 2005, pages 2
   CHAN ET AL., CLIN CHEM, vol. 52, 2006, pages 12
   NYGREN ET AL., CLIN CHEM, vol. 56, 2010, pages 10
   PAPAGEORGIOU ET AL., NAT MED, vol. 4, 2011, pages 510
   TONG ET AL., NAT MED, vol. 18, 2012, pages 1327
   HINDSON ET AL., ANAL CHEM, vol. 83, 2011, pages 8604
   WHITE ET AL., PLOS ONE, vol. 7, no. 9, 2012, pages e45073
   OLD ET AL., RBMONLINE, vol. 15, 2007, pages 227
   TONG ET AL., CLINCHEM, vol. 52, 2006, pages 2194
   CHIM ET AL., CLINCHEM, vol. 54, 2008, pages 500
   PAPAGEORGIO ET AL., AMJPATH, vol. 174, 2009, pages 1609
   LIM ET AL., BMC MEDICAL GENOMICS, vol. 7, 2014, pages 1
   YEGNASUBRAMANIAN ET AL., NUC ACID RES, vol. 34, 2006, pages e19
   PAPANTONIOU ET AL., PRENAT DIAG, vol. 33, 2013, pages 682
   ZEYBEK ET AL., J OBSTET GYNAECOL RES, vol. 39, 2013, pages 632
   JAKOBSEN ET AL., TRANSFUSION, vol. 53, 2013, pages 1956
   CHEN, AM J MED GENET A, vol. 161A, 2013, pages 1817
   STUMM ET AL., PRENAT DIAGN, vol. 34, 2014, pages 185
   NYGREN ET AL., CLIN CHEM, vol. 56, 2010, pages 1627
   CHAN ET AL., CLIN CHEM, vol. 42, 2006, pages 2211
   STUMM ET AL., PRENAT DIAG, vol. 34, 2014, pages 185
   LEUNG ET AL., PRENAT DIAG, vol. 33, 2013, pages 675
   QU ET AL., AM J PATHOL, vol. 170, 2007, pages 941
   STRUBLE ET AL., FETAL DIAGN THER DEC, vol. 7, 2013
   CHEN ET AL., AM J MED GENET A, vol. 161A, 2013, pages 1817
   SPERLING ET AL., ACTA OBSTET GYNECOL SCAND, vol. 80, 2001, pages 287
The EPO accepts no responsibility for the accuracy of data originating from other authorities; in particular, it does not guarantee that it is complete, up to date or fit for specific purposes.