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EP3628740
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| EP3628740 - EXPRESSION OF RECOMBINANT PROTEINS IN TRICHOPLUSIA NI PUPAE [Right-click to bookmark this link] | Status | The application is deemed to be withdrawn Status updated on 04.06.2021 Database last updated on 21.03.2026 | |
| Former | The application has been published Status updated on 28.02.2020 | Most recent event Tooltip | 04.06.2021 | Application deemed to be withdrawn | published on 07.07.2021 [2021/27] | Applicant(s) | For all designated states Alternative Gene Expression, S.L. Centro empresarial Parque Cientifico y Technológico Universidad Politécnica de Madrid Campus de Montegancedo 28223 Pozuelo de Alarcón - Madrid / ES | [2020/22] |
| Former [2020/14] | For all designated states Alternative Gene Expressions, S.L. Centro empresarial Parque Cientifico y Technológico Universidad Politécnica de Madrid Campus de Montegancedo 28223 Pozuelo de Alarcón - Madrid / ES | Inventor(s) | 01 /
MARTÍNEZ ESCRIBANO, José Ángel Centro empresarial Parque Cientifico y Tecnológico Universidad Politécnica de Madrid Campus de Montegancedo 28223 Pozuelo de Alarcón - Madrid / ES | 02 /
ALVARADO FRADUA, Carmen Centro empresarial Parque Cientifico y Tecnológico Universidad Politécnica de Madrid Campus de Montegancedo 28223 Pozuelo de Alarcón - Madrid / ES | 03 /
REYTOR SAAVEDRA, Edel Centro empresarial Parque Cientifico y Tecnológico Universidad Politécnica de Madrid Campus de Montegancedo 28223 Pozuelo de Alarcón - Madrid / ES | 04 /
CID FERNANDEZ, Miguel Centro empresarial Parque Cientifico y Tecnológico Universidad Politécnica de Madrid Campus de Montegancedo 28223 Pozuelo de Alarcón - Madrid / ES | [2020/14] | Representative(s) | Hoffmann Eitle Patent- und Rechtsanwälte PartmbB Arabellastraße 30 81925 München / DE | [2020/14] | Application number, filing date | 19206848.4 | 19.09.2016 | [2020/14] | Priority number, date | EP20150382451 | 17.09.2015 Original published format: EP 15382451 | [2020/14] | Filing language | EN | Procedural language | EN | Publication | Type: | A2 Application without search report | No.: | EP3628740 | Date: | 01.04.2020 | Language: | EN | [2020/14] | Type: | A3 Search report | No.: | EP3628740 | Date: | 29.07.2020 | Language: | EN | [2020/31] | Search report(s) | (Supplementary) European search report - dispatched on: | EP | 26.06.2020 | Classification | IPC: | C12N15/866, A01K67/033, C12N15/85, A61M5/20, B01L99/00 | [2020/14] | CPC: |
C12N15/86 (EP,IL,KR,US);
C12N15/8509 (EP,IL,KR,US);
A01K67/68 (EP,IL,KR,US);
A61M5/20 (EP,IL,KR,US);
A01K2207/05 (IL);
A01K2227/706 (EP,IL,KR,US);
| Designated contracting states | AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LI, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR [2020/14] | Title | German: | EXPRESSION VON REKOMBINANTEN PROTEINEN IN DER TRICHOPLUSIA-NI-PUPPE | [2020/14] | English: | EXPRESSION OF RECOMBINANT PROTEINS IN TRICHOPLUSIA NI PUPAE | [2020/14] | French: | EXPRESSION DE PROTÉINES DE RECOMBINAISON DANS DES PUPES DE TRICHOPLUSIA NI | [2020/14] | Examination procedure | 30.01.2021 | Application deemed to be withdrawn, date of legal effect [2021/27] | 17.02.2021 | Despatch of communication that the application is deemed to be withdrawn, reason: examination fee not paid in time [2021/27] | Parent application(s) Tooltip | EP16766970.4 / EP3350334 | Fees paid | Renewal fee | 04.11.2019 | Renewal fee patent year 03 | 04.11.2019 | Renewal fee patent year 04 | 14.09.2020 | Renewal fee patent year 05 |
| Opt-out from the exclusive Tooltip competence of the Unified Patent Court | See the Register of the Unified Patent Court for opt-out data | ||
| Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court. | Documents cited: | Search | [A] WLTJE MICHAEL ET AL: "Transgenic protein production in silkworm silk glands requires cathepsin and chitinase of Autographa californica multicapsid nucleopolyhedrovirus.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY MAY 2014, vol. 98, no. 10, May 2014 (2014-05-01), pages 4571 - 4580, XP002756203, ISSN: 1432-0614 DOI: http://dx.doi.org/10.1007/s00253-014-5543-8 | [A] MOTOHASHI T ET AL: "Efficient large-scale protein production of larvae and pupae of silkworm by Bombyx mori nuclear polyhedrosis virus bacmid system", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 326, no. 3, 21 January 2005 (2005-01-21), pages 564 - 569, XP004679934, ISSN: 0006-291X, DOI: 10.1016/J.BBRC.2004.11.060 DOI: http://dx.doi.org/10.1016/j.bbrc.2004.11.060 | [IY] HELLERS M ET AL: "Expression of post-translational processing of preprocecropin A using a baculovirus vector.", EUROPEAN JOURNAL OF BIOCHEMISTRY / FEBS 15 JUL 1991, vol. 199, no. 2, 15 July 1991 (1991-07-15), pages 435 - 439, XP002756204, ISSN: 0014-2956 DOI: http://dx.doi.org/10.1111/j.1432-1033.1991.tb16141.x | [A] DATABASE Taxonomy [online] "Autographa californica nucleopolyhedrovirus", XP002756205, retrieved from NCBI Database accession no. 46015 | [A] DATABASE Taxonomy [online] "Autographa californica nuclear polyhedrosis virus (AcMNPV)", XP002756206, retrieved from UniProt Database accession no. 46015 | [Y] GMEZ-SEBASTIN SILVIA ET AL: "Significant productivity improvement of the baculovirus expression vector system by engineering a novel expression cassette.", PLOS ONE 2014, vol. 9, no. 5, E96562, 2014, pages 1 - 10, XP002756207, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0096562 DOI: http://dx.doi.org/10.1371/journal.pone.0096562 | [Y] JINN TZYY-RONG ET AL: "Aerosol infectivity of a Baculovirus to Trichoplusia ni larvae: An alternative larval inoculation strategy for recombinant protein production.", BIOTECHNOLOGY PROGRESS 2009 MAR-APR, vol. 25, no. 2, March 2009 (2009-03-01), pages 384 - 389, XP002756208, ISSN: 1520-6033 DOI: http://dx.doi.org/10.1002/btpr.148 | [A] ANONYMOUS: "Bac-to-Bac TOPO Expression System. An efficient cloning and site-specific transposition system to generate recombinant baculovirus for high-level protein expression Catalog nos. A11101, A11100", 15 December 2008 (2008-12-15), pages i - 51, XP002756209, Retrieved from the Internet | by applicant | WO2012168493 | WO2012168492 | WO2014086981 | NETTLESHIP, J.E.ASSENBERG, R.DIPROSE, J.M.RAHMAN-HUQ, N.OWENS, RJ: "Recent advances in the production of proteins in insect and mammalian cells for structural biology", J. STRUCT. BIOL., vol. 172, 2010, pages 55 - 65, XP002665914, DOI: 10.1016/j.jsb.2010.02.006 DOI: http://dx.doi.org/10.1016/j.jsb.2010.02.006 | GOMEZ-CASADO EGOMEZ-SEBASTIAN SNUNEZ MCLASA-COVARRUBIAS RMARTMEZ-PULGARM SESCRIBANO JM: "Insect larvae biofactories as a platform for influenza vaccine production", PROTEIN EXPR PURIF., vol. 79, 2011, pages 35 - 43, XP002677580, DOI: 10.1016/j.pep.2011.03.007 DOI: http://dx.doi.org/10.1016/j.pep.2011.03.007 | SMITH, G.E.M.D. SUMMERSM.J. FRASER: "Production of human beta interferon in insect cells infected with a baculovirus expression vector", MOL. CELL. BIOL., vol. 3, 1983, pages 2156 - 21 65 | WANG, DNLIU, JWYANG, GZZHANG, WJWU, XF: "Cloning of anti-LPS factor cDNA from Tachypleus tridentatus, expression in Bombyx mori larvae and its biological activity in vitro", MOLECULAR BIOTECHNOLOGY, vol. 21, no. 1, 2002, pages 1 - 7 | WU, XFKAMEI, KSATO, HSATO, STAKANO, RICHIDA, MMORI, HHARA, S.: "High-level expression of human acidic fibroblast growth factor and basic fibroblast growth factor in silkworm (Bombyx mori L.) using recombinant baculovirus", PROTEIN EXPRESSION AND PURIFICATION, vol. 21, no. 1, 2001, pages 192 - 200 | KULAKOSKY, PCHUGHES, PRWOOD, HA: "N-linked glycosylation of a baculovirus-expressed recombinant glycoprotein in insect larvae and tissue culture cells", GLYCOBIOLOGY, vol. 8, no. 7, 1998, pages 741 - 745, XP002601403 | SUZUKI, TKANAYA, TOKAZAKI, HOGAWA, KUSAMI, AWATANABE, HKADONOOKUDA, KYAMAKAWA, MSATO, HMORI, H: "Efficient protein production using a Bombyx mori nuclear polyhedrosis virus lacking the cysteine proteinase gene", JOURNAL OF GENERAL VIROLOGY, vol. 78, 1997, pages 3073 - 3080, XP002588420 | SUMATHY, SPALHAN, VBGOPINATHAN, KP: "Expression of human growth hormone in silkworm larvae through recombinant Bombyx mori nuclear polyhedrosis virus", PROTEIN EXPRESSION AND PURIFICATION, vol. 7, no. 3, 1996, pages 262 - 268 | U. REISB. BLUMB.U. VON SPECHTH. DOMDEYJ. COLLINS: "Antibody production in silkworm cells and silkworm larvae infected with a dual recombinant Bombyx mori nuclear polyhedrosis virus", BIOTECHNOLOGY, vol. 10, 1992, pages 910 - 912, XP001525639 | PEREZ-MARTIN, E.GOMEZ-SEBASTIAN, S.ARGILAGUET, J.M.SIBILA, M.FORT, M.NOFRARIAS, M.KURTZ, S.ESCRIBANO, J.M.SEGALES, J.RODRIGUEZ, F.: "Immunity conferred by an experimental vaccine based on the recombinant PCV2 Cap protein expressed in Trichoplusia ni-larvae", VACCINE, vol. 28, no. 11, 2010, pages 2340 - 2349, XP026924744, DOI: 10.1016/j.vaccine.2009.12.061 DOI: http://dx.doi.org/10.1016/j.vaccine.2009.12.061 | GOMEZ-CASADO EGOMEZ-SEBASTIAN SNUNEZ MCLASA-COVARRUBIAS RMARTMEZ-PULGARIN SESCRIBANO JM: "Insect larvae biofactories as a platform for influenza vaccine production", PROTEIN EXPR. PURIF, vol. 79, 2011, pages 35 - 43, XP002677580, DOI: 10.1016/j.pep.2011.03.007 DOI: http://dx.doi.org/10.1016/j.pep.2011.03.007 | MEDIN, JAHUNT, LGATHY, KEVANS, RKCOLEMAN, MS: "Efficient, low-cost protein factories - expression of human adenosine-deaminase in baculovirus-infected insect larvae", PROCEEDINGS QF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 87, no. 7, 1990, pages 2760 - 2764, XP001121346, DOI: 10.1073/pnas.87.7.2760 DOI: http://dx.doi.org/10.1073/pnas.87.7.2760 | SHAFER, ALKATZ, JBEEMISSE, KA: "Development and validation of a competitive enzyme-linked immunosorbent assay for detection of type A influenza antibodies in avian sera", AVIAN DISEASES, vol. 42, no. 1, 1998, pages 28 - 34, XP009073994, DOI: 10.2307/1592573 DOI: http://dx.doi.org/10.2307/1592573 | CHA, HJPHAM, MQRAO, GBENTLEY, WE: "Expression of green fluorescent protein in insect larvae and its application for heterologous protein production", BIOTECHNOLOGY AND BIOENGINEERING, vol. 56, no. 3, 1997, pages 239 - 247, XP002337510, DOI: 10.1002/(SICI)1097-0290(19971105)56:3<239::AID-BIT1>3.0.CO;2-K DOI: http://dx.doi.org/10.1002/(SICI)1097-0290(19971105)56:3<239::AID-BIT1>3.0.CO;2-K | BURDEN, JPHAILS, RSWINDASS, JDSUNER, MMCORY, JS: "Infectivity, speed of kill, and productivity of a baculovirus expressing the itch mite toxin txp-1 in second and fourth instar larvae of Trichoplusia ni", JOURNAL QF INVERTEBRATE PATHOLOGY, vol. 75, no. 3, 2000, pages 226 - 236 | PEREZ-FILGUEIRA, D. M.RESINO-TALAVAN, P.CUBILLOS, C.ANGULO, I.BARDERAS, M. G.BARCENA, J.ESCRIBANO, J. M.: "Development of a low-cost, insect larvae-derived recombinant subunit vaccine against RHDV", VIROLOGY, vol. 364, no. 2, 2007, pages 422 - 430, XP022106648, DOI: 10.1016/j.virol.2007.03.016 DOI: http://dx.doi.org/10.1016/j.virol.2007.03.016 | PEREZ-FILGUEIRA, D. A.GONZALEZ-CAMACHO, F.GALLARDO, C.RESINO-TALAVAN, P.BLANCO, E.GOMEZ-CASADO, E.ALONSO, C.ESCRIBANO, J. M.: "Optimization and validation of recombinant serological tests for African swine fever diagnosis based on detection of the p30 protein produced in Trichoplusia ni larvae", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 44, no. 9, 2006, pages 3114 - 3121 | HELLERS, MGUNNE, HSTEINER, H.: "Expression and posttranslational processing of preprocecropin-a using a baculovirus vector", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 199, no. 2, 1991, pages 435 - 439, XP002756204, DOI: 10.1111/j.1432-1033.1991.tb16141.x DOI: http://dx.doi.org/10.1111/j.1432-1033.1991.tb16141.x | AKIHIRO USAMISEIJI ISHIYAMACHIAKI ENOMOTOHIRONOBU OKAZAKIKEIKO HIGUCHIMASHAHIRO IKEDATAKESHI YAMAMOTOMUTSUMI SUGAIYUKIKO ISHIKAWAY: "Comparison of recombinant protein expression in a baculovirus system in insect cells (Sf9) and silkwonn", J. BIOCHEM., vol. 149, no. 2, 2011, pages 219 - 227 | CHAZARRA SAZNAR-CERVANTES SSANCHEZ-DEL-CAMPO LCABEZAS-HERRERA JXIAOFENG WCENIS JLRODRIGUEZ-LPEZ JN: "Purification and kinetic properties of human recombinant dihydrofolate reductase produced in Bombyx mori chrysalides", APPL BIOCHEM BIOTECHNOL., vol. 162, no. 7, November 2010 (2010-11-01), pages 1834 - 46 | JOURNAL OF GENERAL VIROLOGY, vol. 73, 1992, pages 3195 - 320 | HELLERS, M.STEINER, H., INSECT BIOCHEM. MOLEC. BIOL., vol. 22, 1992, pages 35 - 39 | "GenBank", Database accession no. AJ 277616.1 | "NCBI", Database accession no. NC _016896.1 |