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Extract from the Register of European Patents

EP About this file: EP4191234

EP4191234 - METHOD OF ANALYSING A MIXED FLUORESCENCE RESPONSE OF A PLURALITY OF FLUOROPHORES, FLUORESCENCE ANALYSER, FLUORESCENCE MICROSCOPE AND COMPUTER PROGRAM [Right-click to bookmark this link]
StatusRequest for examination was made
Status updated on  08.12.2023
Database last updated on 06.07.2024
FormerThe application has been published
Status updated on  05.05.2023
Most recent event   Tooltip12.04.2024Change - representative 
Applicant(s)For all designated states
Leica Microsystems CMS GmbH
Ernst-Leitz-Strasse 17-37
35578 Wetzlar / DE
[2023/23]
Inventor(s)01 / DEISSLER, Benjamin
35510 Butzbach / DE
02 / SCHUMANN, Christian
35423 Lich / DE
03 / RITSCHEL, Kai
35452 Heuchelheim / DE
04 / BELJAN, Mate
61440 Oberursel / DE
05 / FOUQUET, Wernher
68167 Mannheim / DE
06 / COJOC, Gheorghe
01277 Dresden / DE
 [2023/23]
Representative(s)Schaumburg und Partner Patentanwälte mbB
Mauerkircherstraße 31
81679 München / DE
[N/P]
Former [2023/23]DehnsGermany Partnerschaft von Patentanwälten
Theresienstraße 6-8
80333 München / DE
Application number, filing date21212550.406.12.2021
[2023/23]
Filing languageEN
Procedural languageEN
PublicationType: A1 Application with search report 
No.:EP4191234
Date:07.06.2023
Language:EN
[2023/23]
Search report(s)(Supplementary) European search report - dispatched on:EP13.05.2022
ClassificationIPC:G01N21/64, G01J3/44, G02B21/16
[2023/23]
CPC:
G01N21/6458 (EP); G01N21/64 (CN,US); G01J3/4406 (EP);
G01N21/6428 (EP); G01N2021/6441 (EP); G01N2201/127 (US);
G02B21/16 (EP); G02B21/367 (EP) (-)
Designated contracting statesAL,   AT,   BE,   BG,   CH,   CY,   CZ,   DE,   DK,   EE,   ES,   FI,   FR,   GB,   GR,   HR,   HU,   IE,   IS,   IT,   LI,   LT,   LU,   LV,   MC,   MK,   MT,   NL,   NO,   PL,   PT,   RO,   RS,   SE,   SI,   SK,   SM,   TR [2024/02]
Former [2023/23]AL,  AT,  BE,  BG,  CH,  CY,  CZ,  DE,  DK,  EE,  ES,  FI,  FR,  GB,  GR,  HR,  HU,  IE,  IS,  IT,  LI,  LT,  LU,  LV,  MC,  MK,  MT,  NL,  NO,  PL,  PT,  RO,  RS,  SE,  SI,  SK,  SM,  TR 
Extension statesBANot yet paid
MENot yet paid
Validation statesKHNot yet paid
MANot yet paid
MDNot yet paid
TNNot yet paid
TitleGerman:VERFAHREN ZUR ANALYSE EINER GEMISCHTEN FLUORESZENZANTWORT MEHRERER FLUOROPHORE, FLUORESZENZANALYSATOR, FLUORESZENZMIKROSKOP UND COMPUTERPROGRAMM[2023/23]
English:METHOD OF ANALYSING A MIXED FLUORESCENCE RESPONSE OF A PLURALITY OF FLUOROPHORES, FLUORESCENCE ANALYSER, FLUORESCENCE MICROSCOPE AND COMPUTER PROGRAM[2023/23]
French:PROCÉDÉ D'ANALYSE D'UNE RÉPONSE DE FLUORESCENCE MIXTE D'UNE PLURALITÉ DE FLUOROPHORES, ANALYSEUR DE FLUORESCENCE, MICROSCOPE À FLUORESCENCE ET PROGRAMME INFORMATIQUE[2023/23]
Examination procedure15.11.2023Observations by third parties
06.12.2023Amendment by applicant (claims and/or description)
06.12.2023Examination requested  [2024/02]
06.12.2023Date on which the examining division has become responsible
Fees paidRenewal fee
26.12.2023Renewal fee patent year 03
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See the Register of the Unified Patent Court for opt-out data
Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Documents cited:Search[A]US6687000  (WHITE JOHN G [US]) [A] 1-16 * figures 1-2 *;
 [X]US2005243313  (NEHER ERWIN [DE], et al) [X] 1,3,4,6-10,12-16 * [0001]-[0002], [0010], [0013], [0015], [0022], [0026]-[0027], [0048], [0051], [0054]-[0055];; figure 1 *;
 [A]US2021208076  (CHANG JAE-BYUM [KR], et al) [A] 1-16 * [0047]-[0048], [0062] *;
 [X]  - Mcrae Tristan D. ET AL, "Robust blind spectral unmixing for fluorescence microscopy using unsupervised learning", bioRxiv, doi:10.1101/797993, (20191009), URL: https://www.biorxiv.org/content/10.1101/797993v1.full.pdf, (20220117), XP055879880 [X] 1-9,11-16 * figures 2-6 *

DOI:   http://dx.doi.org/10.1101/797993
 [A]  - HUANG SHAOSEN ET AL, "Nonnegative matrix factorization using target-to-background contrast for fluorescence unmixing", 2013 IEEE INTERNATIONAL CONFERENCE ON MEDICAL IMAGING PHYSICS AND ENGINEERING, IEEE, (20131019), doi:10.1109/ICMIPE.2013.6864553, pages 287 - 292, XP032625511 [A] 1-16 * Section 3 *

DOI:   http://dx.doi.org/10.1109/ICMIPE.2013.6864553
 [A]  - MURAD MEGJHANI ET AL, "Morphologically constrained spectral unmixing by dictionary learning for multiplex fluorescence microscopy", BIOINFORMATICS, GB, (20170715), vol. 33, no. 14, doi:10.1093/bioinformatics/btx108, ISSN 1367-4803, pages 2182 - 2190, XP055589656 [A] 1-16 * Sections 2-3;; figures 1-3 *

DOI:   http://dx.doi.org/10.1093/bioinformatics/btx108
 [A]  - PU YANG ET AL, "Evaluation of algorithm methods for fluorescence spectra of cancerous and normal human tissues", PROGRESS IN BIOMEDICAL OPTICS AND IMAGING, SPIE - INTERNATIONAL SOCIETY FOR OPTICAL ENGINEERING, BELLINGHAM, WA, US, (20160307), vol. 9703, doi:10.1117/12.2205628, ISSN 1605-7422, ISBN 978-1-5106-0027-0, pages 97031M - 97031M, XP060065021 [A] 1-16 * Section 2.2 *

DOI:   http://dx.doi.org/10.1117/12.2205628
 [A]  - JERILYN A. TIMLIN, "Imaging multiple endogenous and exogenous fluorescent species in cells and tissues", PROCEEDINGS OF SPIE, VISUAL COMMUNICATIONS AND IMAGE PROCESSING 2005, Visual Communications and Image Processing 2005, 2005, Beijing, China, (20060101), vol. 6088, doi:10.1117/12.647228, ISSN 0277-786X, page 608805, XP055013152 [A] 1-16 * figure 1 *

DOI:   http://dx.doi.org/10.1117/12.647228
 [A]  - JAMES R. MANSFIELD ET AL, "Autofluorescence removal, multiplexing, and automated analysis methods for in-vivo fluorescence imaging", JOURNAL OF BIOMEDICAL OPTICS, 1000 20th St. Bellingham WA 98225-6705 USA, (20050101), vol. 10, no. 4, doi:10.1117/1.2032458, ISSN 1083-3668, page 041207, XP055269855 [A] 1-16 * Section 3.1 *

DOI:   http://dx.doi.org/10.1117/1.2032458
by applicantEP3721279
 WO2021185557
    - T. ZIMMERMANN, "Spectral Imaging and Linear Unmixing in Light Microscopy", Adv. Biochem. Eng. Biotechnol., (20050000), vol. 95, doi:10.1007/b102216, pages 245 - 265, XP055589696

DOI:   http://dx.doi.org/10.1007/b102216
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