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Extract from the Register of European Patents

EP About this file: EP0999277

EP0999277 - Method for expression of recombinant proteins through the use of the effects of stress and stress-response mechanisms in the presence of compatible solutes [Right-click to bookmark this link]
StatusThe application is deemed to be withdrawn
Status updated on  20.07.2001
Database last updated on 07.10.2024
Most recent event   Tooltip19.03.2010Change - representativepublished on 21.04.2010  [2010/16]
Applicant(s)For all designated states
Barth, Stefan, Dr. rer. nat.
c/o Klinik I für innere Medizin der Universität zu Köln Joseph-Stelzmann-Strasse 9
50931 Köln / DE
[N/P]
Former [2000/19]For all designated states
Barth, Stefan, Dr. rer. nat.
c/o Klinik I für innere Medizin der Universität zu Köln, Joseph-Stelzmann-Strasse 9
50931 Köln / DE
Inventor(s)01 / Barth, Stefan, Dr.Dr.
Zülpicher Wall 40
50674 Köln / DE
02 / Engert, Andreas, Dr.
Spichernstrasse 18
50672 Köln / DE
03 / Louis, Petra
Thusneldastrasse 24
53117 Bonn / DE
04 / Galinski, Erwin A., Prof.Dr.
Sperberweg 27
48308 Senden / DE
05 / Huhn, Michael, Dr.
Opladener Strasse 106a
51375 Leverkusen / DE
[2000/41]
Former [2000/19]01 / Barth, Stefan, Dr.Dr.
Zülpicher Wall 40
50674 Köln / DE
02 / Engert, Andreas, Dr.
Spichernstrasse 18
50672 Köln / DE
03 / Louis, Petra
Thusneldastrasse 24
53117 Bonn / DE
04 / Galinski, Erwin, Prof.Dr.
Melbweg 36a
53127 Bonn / DE
05 / Huhn, Michael, Dr.
Opladener Strasse 106a
51375 Leverkusen / DE
Representative(s)dompatent von Kreisler Selting Werner - Partnerschaft von Patent- und Rechtsanwälten mbB
Deichmannhaus am Dom
Bahnhofsvorplatz 1
50667 Köln / DE
[N/P]
Former [2010/15]von Kreisler Selting Werner
Deichmannhaus am Dom Bahnhofsvorplatz 1
50667 Köln / DE
Former [2000/19]Meyers, Hans-Wilhelm, Dr.Dipl.-Chem., et al
Patentanwälte von Kreisler-Selting-Werner Postfach 10 22 41
50462 Köln / DE
Application number, filing date98121243.407.11.1998
[2000/19]
Filing languageDE
Procedural languageDE
PublicationType: A1 Application with search report 
No.:EP0999277
Date:10.05.2000
Language:DE
[2000/19]
Search report(s)(Supplementary) European search report - dispatched on:EP27.04.1999
ClassificationIPC:C12N15/70, C12N1/38, C12N15/62, C12P21/02
[2000/19]
CPC:
C12N15/52 (EP); C07K16/2866 (EP); C07K16/2878 (EP);
C12N1/38 (EP); C12N15/625 (EP); C12P21/02 (EP);
C07K2319/21 (EP); C07K2319/55 (EP) (-)
Designated contracting states(deleted) [2001/03]
Former [2000/19]AT,  BE,  CH,  CY,  DE,  DK,  ES,  FI,  FR,  GB,  GR,  IE,  IT,  LI,  LU,  MC,  NL,  PT,  SE 
TitleGerman:Verfahren zur Expression rekombinanter Proteine unter Ausnutzung der Wirkung von Stress und Stressantwortmechanismen in Gegenwart kompatibler Solute[2000/19]
English:Method for expression of recombinant proteins through the use of the effects of stress and stress-response mechanisms in the presence of compatible solutes[2000/19]
French:Procédé pour l'expression de protéines recombinantes par exploitation des effets de stress et des mécanismes de réponse au stress en présence de solutés compatibles[2000/19]
Examination procedure11.11.2000Application deemed to be withdrawn, date of legal effect  [2001/36]
23.02.2001Despatch of communication that the application is deemed to be withdrawn, reason: examination fee not paid in time  [2001/36]
Fees paidPenalty fee
Penalty fee Rule 85a EPC 1973
18.12.2000AT   M01   Not yet paid
18.12.2000BE   M01   Not yet paid
18.12.2000CH   M01   Not yet paid
18.12.2000CY   M01   Not yet paid
18.12.2000DE   M01   Not yet paid
18.12.2000DK   M01   Not yet paid
18.12.2000ES   M01   Not yet paid
18.12.2000FI   M01   Not yet paid
18.12.2000FR   M01   Not yet paid
18.12.2000GB   M01   Not yet paid
18.12.2000GR   M01   Not yet paid
18.12.2000IE   M01   Not yet paid
18.12.2000IT   M01   Not yet paid
18.12.2000LU   M01   Not yet paid
18.12.2000MC   M01   Not yet paid
18.12.2000NL   M01   Not yet paid
18.12.2000PT   M01   Not yet paid
18.12.2000SE   M01   Not yet paid
Penalty fee Rule 85b EPC 1973
18.12.2000M01   Not yet paid
Additional fee for renewal fee
30.11.200003   M06   Not yet paid
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Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court.
Documents cited:Search[A]DE19610972  (GALINSKI ERWIN A DR ;LOUIS PETRA (DE)) [A] 4,6,7 * abstract *;
 [XY]  - HUHN, M. ET AL., "Highly efficient periplasmic expression of functional Pseudomonas Exotoxin-based immunotoxins.", ANNALS OF HEMATOLOGY, (19981025), vol. 77, no. Suppl. II, pages S84 - Abstr.331, XP002099619 [X] 1-11,15,16 * the whole document * [Y] 12-14
 [YA]  - HERBST B ET AL, "pOSEX: vectors for osmotically controlled and finely tuned gene expression in Escherichia coli", GENE, (19941230), vol. 151, no. 1, page 137-142, XP004042625 [Y] 12 * abstract * * page 138, column L, paragraph 1 * [A] 13

DOI:   http://dx.doi.org/10.1016/0378-1119(94)90644-0
 [YA]  - MATTHEY, B. ET AL., "pMB1.1, a new pET-based vector for high efficiency expression of ETA' fusion toxins.", EUROPEAN JOURNAL OF CANCER, (199706), vol. 33, no. Suppl.5, page 129, XP002099620 [Y] 13 * the whole document * [A] 9,10

DOI:   http://dx.doi.org/10.1016/S0959-8049(96)00342-5
 [Y]  - SCHEIN C ET AL, "Formation of soluble recombinant proteins in Escherichia coli is favored by lower growth temperature", BIO/TECHNOLOGY, (198803), vol. 6, pages 291 - 294, XP002092249 [Y] 14 * abstract *

DOI:   http://dx.doi.org/10.1038/nbt0388-291
 [A]  - BLACKWELL, J.R. ET AL., "A novel strategy for production of a highly expressed recombinant protein in an active form", FEBS LETTERS, (199112), vol. 295, no. 1-3, pages 10 - 12, XP002099621 [A] * abstract * * page 11, column R, line 1 - line 21 *

DOI:   http://dx.doi.org/10.1016/0014-5793(91)81372-F
 [A]  - FRINGS E ET AL, "Production of hydroxyectoine: high cell-density cultivation and osmotic downshock of Marinococcus strain M52", JOURNAL OF BIOTECHNOLOGY, (19951121), vol. 43, no. 1, page 53-61, XP004036886 [A] 1-5 * das ganze Dokument, ins besondere Seite 54, linke Spalte, Absatz 3, Seite 56-57, unter "Results" - absatz 1, und der erste Absatz der Diskussion (Seite 59-60) *

DOI:   http://dx.doi.org/10.1016/0168-1656(95)00119-2
 [A]  - DA COSTA, M.S. ET AL., "An overview of the role and diversity of compatible solutes in bacteria and archaea.", ADVANCES IN BIOCHEMICAL ENGENEERING/BIOTECHNOLOGY, (19980101), vol. 61, XP002099622 [A] 1-3 * paragraph [03.2]; figures 8A,9A *
 [A]  - MAY, G. ET AL., "Binding protein dependent transport of glycine betaine and its osmotic regulation in Escherichia coli K12", MOLECULAR AND GENERAL GENETICS, (198611), vol. 205, no. 2, pages 225 - 233, XP002099623 [A] * the whole document *

DOI:   http://dx.doi.org/10.1007/BF00430432
 [A]  - SCHEIN C H, "PRODUCTION OF SOLUBLE RECOMBINANT PROTEINS IN BACTERIA", BIO/TECHNOLOGY, (19891101), vol. 7, no. 11, pages 1141 - 1149, XP000068927 [A] * abstract * * page 1144, column R, paragraph 5 * * page 1146, column L, line 2 *
 [T]  - MATTHEY, B. ET AL., "A new series of pET-derived vectors for high efficiency expression of Pseudomonas exotoxin-based fusion proteins", GENE, (1999), vol. 229, pages 145 - 53, XP002099624 [T] * the whole document *

DOI:   http://dx.doi.org/10.1016/S0378-1119(99)00038-4
The EPO accepts no responsibility for the accuracy of data originating from other authorities; in particular, it does not guarantee that it is complete, up to date or fit for specific purposes.