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EP4291647
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EP About this file: EP4291647
| EP4291647 - SUBTILISIN VARIANTS AND THEIR USE [Right-click to bookmark this link] | Status | The patent has been granted Status updated on 16.05.2025 Database last updated on 07.04.2026 | |
| Former | Grant of patent is intended Status updated on 21.01.2025 | ||
| Former | Request for examination was made Status updated on 17.11.2023 | ||
| Former | The international publication has been made Status updated on 19.08.2022 | ||
| Former | unknown Status updated on 28.02.2022 | Most recent event Tooltip | 30.01.2026 | Lapse of the patent in a contracting state New state(s): PL, SK | published on 04.03.2026 [2026/10] | Applicant(s) | For all designated states Fresenius Kabi iPSUM S.r.l. Via Roma, 108 20051 Cassina De Pecchi - Milano / IT | [2025/36] |
| Former [2023/51] | For all designated states Fresenius Kabi iPSUM S.r.l. Via Roma, 108 20060 Cassina de' Pecchi - Milano / IT | Inventor(s) | 01 /
NUIJENS, Timo 6167 RD Geleen / NL | 02 /
MATEMAN, Jurgen 6167 RD Geleen / NL | 03 /
DE VISSER, Rowin 6167 RD Geleen / NL | 04 /
CABRI, Walter 20060 Cassina de' Pecchi - MILANO / IT | 05 /
WALLRAVEN, Kerstin 6167 RD Geleen / NL | 06 /
WIJKER, Elvira 6167 RD Geleen / NL | 07 /
RICCI, Antonio 20060 Cassina de' Pecchi - MILANO / IT | 08 /
TOPLAK, Ana 6167 RD Geleen / NL | [2023/51] | Representative(s) | Fresenius Kabi Deutschland GmbH Patent Department Borkenberg 14 61440 Oberursel / DE | [2023/51] | Application number, filing date | 22704910.3 | 09.02.2022 | [2023/51] | WO2022EP53121 | Priority number, date | EP20210156989 | 12.02.2021 Original published format: EP 21156989 | EP20210176039 | 26.05.2021 Original published format: EP 21176039 | [2023/51] | Filing language | EN | Procedural language | EN | Publication | Type: | A1 Application with search report | No.: | WO2022171667 | Date: | 18.08.2022 | Language: | EN | [2022/33] | Type: | A1 Application with search report | No.: | EP4291647 | Date: | 20.12.2023 | Language: | EN | The application published by WIPO in one of the EPO official languages on 18.08.2022 takes the place of the publication of the European patent application. | [2023/51] | Type: | B1 Patent specification | No.: | EP4291647 | Date: | 18.06.2025 | Language: | EN | [2025/25] | Search report(s) | International search report - published on: | EP | 18.08.2022 | Classification | IPC: | C12N9/54 | [2025/04] | CPC: |
C12N9/54 (EP,KR,US);
C12P21/02 (US);
C07K1/10 (KR);
C12Y304/21062 (EP,KR,US)
|
| Former IPC [2023/51] | C12N9/54, C07K14/605, C07K14/585 | Designated contracting states | AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LI, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR [2023/51]
| Extension states | BA | Not yet paid | ME | Not yet paid | Validation states | KH | Not yet paid | MA | Not yet paid | MD | Not yet paid | TN | Not yet paid | Title | German: | SUBTILISINVARIANTEN UND IHRE VERWENDUNG | [2025/04] | English: | SUBTILISIN VARIANTS AND THEIR USE | [2023/51] | French: | VARIANTES DE SUBTILISINE ET LEURS UTILISATIONS | [2025/04] |
| Former [2023/51] | SUBTILISINVARIANTEN UND DEREN VERWENDUNG | ||
| Former [2023/51] | VARIANTS DE SUBTILISINE ET LEUR UTILISATION | Entry into regional phase | 26.07.2023 | National basic fee paid | 26.07.2023 | Designation fee(s) paid | 26.07.2023 | Examination fee paid | Examination procedure | 26.07.2023 | Examination requested [2023/51] | 26.07.2023 | Date on which the examining division has become responsible | 18.03.2024 | Amendment by applicant (claims and/or description) | 22.01.2025 | Communication of intention to grant the patent | 13.05.2025 | Fee for grant paid | 13.05.2025 | Fee for publishing/printing paid | 13.05.2025 | Receipt of the translation of the claim(s) | Fees paid | Renewal fee | 15.02.2024 | Renewal fee patent year 03 | 25.02.2025 | Renewal fee patent year 04 |
| Opt-out from the exclusive Tooltip competence of the Unified Patent Court | See the Register of the Unified Patent Court for opt-out data | ||
| Responsibility for the accuracy, completeness or quality of the data displayed under the link provided lies entirely with the Unified Patent Court. | Lapses during opposition Tooltip | HR | 18.06.2025 | PL | 18.06.2025 | SK | 18.06.2025 | SM | 18.06.2025 | NO | 18.09.2025 | RS | 18.09.2025 | GR | 19.09.2025 | IS | 18.10.2025 | [2026/10] |
| Former [2026/08] | HR | 18.06.2025 | |
| SM | 18.06.2025 | ||
| NO | 18.09.2025 | ||
| RS | 18.09.2025 | ||
| GR | 19.09.2025 | ||
| IS | 18.10.2025 | ||
| Former [2026/07] | HR | 18.06.2025 | |
| NO | 18.09.2025 | ||
| RS | 18.09.2025 | ||
| GR | 19.09.2025 | ||
| IS | 18.10.2025 | ||
| Former [2025/48] | HR | 18.06.2025 | |
| NO | 18.09.2025 | ||
| RS | 18.09.2025 | ||
| GR | 19.09.2025 | ||
| Former [2025/47] | NO | 18.09.2025 | |
| GR | 19.09.2025 | Cited in | International search | [AD] WO2018212658 (ENZYPEP B V et al.) | [AD] WO2019170918 (ENZYPEP B V et al.) | [A] WO2010056640 (DANISCO US INC et al.) | [A] WO0226956 (UNIV MARYLAND BIOTECH INST et al.) | [A] WO9949057 (PROCTER & GAMBLE et al.) | [A] NUIJENS TIMO ET AL: "Natural Occurring and Engineered Enzymes for Peptide Ligation and Cyclization", vol. 7, 29 November 2019 (2019-11-29), XP055818355, Retrieved from the Internet DOI: http://dx.doi.org/10.3389/fchem.2019.00829 | by applicant | US5403737 | WO2016056913 | WO2018212658 | WO2019170895 | WO2019170918 | WO2016006913 | WELLS ET AL.: "introduced five additional mutations to subtiligase, i.e. M50F, N76D, N109S, K213R and N218S, to increase the enzyme's stability", PROC. NATL. ACAD. SCI. USA, vol. 91, 1994, pages 12544 | SILOTO ET AL.: "Site saturation mutagenesis: Methods and applications in protein engineering", BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, vol. 1, 2012, pages 181 - 189, XP055412897, DOI: 10.1016/j.bcab.2012.03.010 DOI: http://dx.doi.org/10.1016/j.bcab.2012.03.010 | NEEDLEMAN, S. B. AND WUNSCH, C. D., J. MOL. BIOL., vol. 48, no. 3, 1970, pages 443 - 453 | RICE, P.LONGDEN, I.BLEASBY, A.: "EMBOSS: The European Molecular Biology Open Software Suite", TRENDS IN GENETICS, vol. 16, no. 6, 2000, pages 276 - 277, XP004200114, DOI: 10.1016/S0168-9525(00)02024-2 DOI: http://dx.doi.org/10.1016/S0168-9525(00)02024-2 | SMITHJOHNSON, GENE, vol. 67, 1988, pages 31 - 40 | GOOD, N.E. ET AL.: "Hydrogen Ion Buffers for Biological Research", BIOCHEMISTRY, vol. 5, no. 2, 1966, pages 467 - 477, XP000881106, DOI: 10.1021/bi00866a011 DOI: http://dx.doi.org/10.1021/bi00866a011 | E.K. LEE, JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, vol. 9, 2010, pages 11 - 18 |